Light signaling precisely controls photomorphogenic development in plants.PHYTOCHROME INTERACTING FACTOR 4 and 5(PIF4 and PIF5)play critical roles in the regulation of this developmental process.In this study,we repor...Light signaling precisely controls photomorphogenic development in plants.PHYTOCHROME INTERACTING FACTOR 4 and 5(PIF4 and PIF5)play critical roles in the regulation of this developmental process.In this study,we report CONSTITUTIVELY PHOTOMORPHOGENIC 1 SUPPRESSOR 6(CSU6)functions as a key regulator of light signaling.Loss of CSU6 function largely rescues the cop1-6 constitutively photomorphogenic phenotype.CSU6 promotes hypocotyl growth in the dark,but inhibits hypocotyl elongation in the light.CSU6 not only associates with the promoter regions of PIF4 and PIF5 to inhibit their expression in the morning,but also directly interacts with both PIF4 and PIF5 to repress their transcriptional activation activity.CSU6 negatively controls a group of PIF4-and PIF5-regulated gene expressions.Mutations in PIF4 and/or PIF5 are epistatic to the loss of CSU6,suggesting that CSU6 acts upstream of PIF4 and PIF5.Taken together,CSU6 promotes light-inhibited hypocotyl elongation by negatively regulating PIF4 and PIF5 transcription and biochemical activity.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos 32100199,31900210,and 31970258)the Peking-Tsinghua Center for Life Sciences(to X.W.D)+4 种基金the Southern University of Science and Technology(to X.W.D)the Jiangsu Natural Science Foundation for Distinguished Young Scholars(Grant No.BK20211525)the Jiangsu"Innovativeand Entrepreneurial Talent"program(to D.X.)the Nanjing Agricultural University(start-up funding to D.X.)the Jiangsu Collaborative Innovation Center for Modern Crop Production(to D.X.).
文摘Light signaling precisely controls photomorphogenic development in plants.PHYTOCHROME INTERACTING FACTOR 4 and 5(PIF4 and PIF5)play critical roles in the regulation of this developmental process.In this study,we report CONSTITUTIVELY PHOTOMORPHOGENIC 1 SUPPRESSOR 6(CSU6)functions as a key regulator of light signaling.Loss of CSU6 function largely rescues the cop1-6 constitutively photomorphogenic phenotype.CSU6 promotes hypocotyl growth in the dark,but inhibits hypocotyl elongation in the light.CSU6 not only associates with the promoter regions of PIF4 and PIF5 to inhibit their expression in the morning,but also directly interacts with both PIF4 and PIF5 to repress their transcriptional activation activity.CSU6 negatively controls a group of PIF4-and PIF5-regulated gene expressions.Mutations in PIF4 and/or PIF5 are epistatic to the loss of CSU6,suggesting that CSU6 acts upstream of PIF4 and PIF5.Taken together,CSU6 promotes light-inhibited hypocotyl elongation by negatively regulating PIF4 and PIF5 transcription and biochemical activity.
