Herein,a novel dual-emission fluorescence sensor array of carbonized polymer dots(CPDs)-Cu^(2+)has been proposed,in which CPDs were prepared by one-pot hydrothermal method and Cu^(2+)acting as a quencher was combined ...Herein,a novel dual-emission fluorescence sensor array of carbonized polymer dots(CPDs)-Cu^(2+)has been proposed,in which CPDs were prepared by one-pot hydrothermal method and Cu^(2+)acting as a quencher was combined with CPDs by electrostatic interaction.Four negatively charged glycosaminoglycans(GAGs)bearing different hydrophilic groups showed variable binding affinities towards CPDs-Cu^(2+).Upon reacting with these GAGs,the different fluorescence response signals of CPDs-Cu^(2+)can be further differentiated by principal component analysis(PCA).The CPDs-Cu^(2+)sensor array,not only allows the identification of four similarly structured GAGs,but also realizes the discrimination of different concentrations of the same GAGs and their mixtures.Remarkably,the identification of GAGs in biological fluids can also be achieved using our proposed single-component sensor array,validating its application potential.This new strategy avoids multiple sensing probes,broadens the application of tongue-mimic sensor arrays and provides a viable idea for the development of single component sensing platforms.展开更多
配电信息物理系统(cyber physical distribution system,CPDS)与主动配电系统具有内在一致性,该融合系统的多维不确定性、深度耦合性使其规划问题更为复杂。文中基于配电信息物理系统在规划中的耦合特性分析,建立计及电压控制功能及通...配电信息物理系统(cyber physical distribution system,CPDS)与主动配电系统具有内在一致性,该融合系统的多维不确定性、深度耦合性使其规划问题更为复杂。文中基于配电信息物理系统在规划中的耦合特性分析,建立计及电压控制功能及通信系统随机故障的CPDS规划模型,提出考虑信息系统随机失效和控制失效的CPDS规划框架,通过优化方案形成和方案概率约束指标评价相结合的融合过程进行求解。以分布式光伏并网且具备电压控制功能的改进IEEE-33节点配电系统为例进行仿真验证,验证所提模型和算法的有效性和实用性,并探讨控制参数、光伏渗透率对配置方案和信息系统随机故障对电压合格概率指标的影响,为配电信息物理系统的规划和改进措施提出建议。展开更多
DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific mono...DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 6-4PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 6-4PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24℃ was much faster than that at 0℃ and 12℃, with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at 12℃ was very slow as compared with that at 24℃, and almost no removal of 6-4PPs was detected after 4 h exposure to white light at 0℃. There was evidence to suggest that temperature-dependent DNA damage and photorepair could have important ecological implications.展开更多
It is highly desired to accurately and selectively detect and image intracellular L-lysine and pH in biological systems because they could act as the biomarkers in certain abnormal conditions and may give us a warning...It is highly desired to accurately and selectively detect and image intracellular L-lysine and pH in biological systems because they could act as the biomarkers in certain abnormal conditions and may give us a warning of the occurrence of diseases.It has been attracted more focuses to design new ratiometric fluorescent probe for monitoring L-lysine and pH to improve detection accuracy.Carbonized polymer dots(CPDs),which possess carbon/polymer hybrid structure rather than pure carbon structure and constitute of a carbon core and large amounts of functional groups/polymer chains on the surface,rise up as a new type of fluorescent nanomaterials and especially display many advantages for bioanalysis.In this study,o-phenylenediamine(o-PD)and poly(styrene-co-maleic anhydride)(PSMA)are used as the precursors to synthesize the desired CPDs through one-step hydrothermal amide method.The prepared CPDs display two well-resolved fluorescence emission bands,i.e.,a very weak emission centered at 470 nm in blue region and a strong emission centered at 558 nm in yellow region.It is found that the two emissions are both responsive to L-lysine based on the surface passivation mechanism,whereas,only the yellow emission is responsive to pH due to the protonation/deprotonation process of the amino groups.Based on the different responsive behaviors,ratiometric detection and imaging of L-lysine and pH are achieved.The prepared ratiometric CPDs probe is successfully applied for L-lysine and pH sensing and imaging at two emission channels in live cell and zebrafish with satisfactory results.展开更多
基金supported by the National Natural Science Foundation of China(22274053)the Fundamental Research Funds for the Central Universities
文摘Herein,a novel dual-emission fluorescence sensor array of carbonized polymer dots(CPDs)-Cu^(2+)has been proposed,in which CPDs were prepared by one-pot hydrothermal method and Cu^(2+)acting as a quencher was combined with CPDs by electrostatic interaction.Four negatively charged glycosaminoglycans(GAGs)bearing different hydrophilic groups showed variable binding affinities towards CPDs-Cu^(2+).Upon reacting with these GAGs,the different fluorescence response signals of CPDs-Cu^(2+)can be further differentiated by principal component analysis(PCA).The CPDs-Cu^(2+)sensor array,not only allows the identification of four similarly structured GAGs,but also realizes the discrimination of different concentrations of the same GAGs and their mixtures.Remarkably,the identification of GAGs in biological fluids can also be achieved using our proposed single-component sensor array,validating its application potential.This new strategy avoids multiple sensing probes,broadens the application of tongue-mimic sensor arrays and provides a viable idea for the development of single component sensing platforms.
文摘配电信息物理系统(cyber physical distribution system,CPDS)与主动配电系统具有内在一致性,该融合系统的多维不确定性、深度耦合性使其规划问题更为复杂。文中基于配电信息物理系统在规划中的耦合特性分析,建立计及电压控制功能及通信系统随机故障的CPDS规划模型,提出考虑信息系统随机失效和控制失效的CPDS规划框架,通过优化方案形成和方案概率约束指标评价相结合的融合过程进行求解。以分布式光伏并网且具备电压控制功能的改进IEEE-33节点配电系统为例进行仿真验证,验证所提模型和算法的有效性和实用性,并探讨控制参数、光伏渗透率对配置方案和信息系统随机故障对电压合格概率指标的影响,为配电信息物理系统的规划和改进措施提出建议。
文摘DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 6-4PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 6-4PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24℃ was much faster than that at 0℃ and 12℃, with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at 12℃ was very slow as compared with that at 24℃, and almost no removal of 6-4PPs was detected after 4 h exposure to white light at 0℃. There was evidence to suggest that temperature-dependent DNA damage and photorepair could have important ecological implications.
基金financially supported by the National Natural Science Foundation of China(Nos.21874001,21575004)the Foundation for Innovation Team of Bioanalytical Chemistry of Anhui Province。
文摘It is highly desired to accurately and selectively detect and image intracellular L-lysine and pH in biological systems because they could act as the biomarkers in certain abnormal conditions and may give us a warning of the occurrence of diseases.It has been attracted more focuses to design new ratiometric fluorescent probe for monitoring L-lysine and pH to improve detection accuracy.Carbonized polymer dots(CPDs),which possess carbon/polymer hybrid structure rather than pure carbon structure and constitute of a carbon core and large amounts of functional groups/polymer chains on the surface,rise up as a new type of fluorescent nanomaterials and especially display many advantages for bioanalysis.In this study,o-phenylenediamine(o-PD)and poly(styrene-co-maleic anhydride)(PSMA)are used as the precursors to synthesize the desired CPDs through one-step hydrothermal amide method.The prepared CPDs display two well-resolved fluorescence emission bands,i.e.,a very weak emission centered at 470 nm in blue region and a strong emission centered at 558 nm in yellow region.It is found that the two emissions are both responsive to L-lysine based on the surface passivation mechanism,whereas,only the yellow emission is responsive to pH due to the protonation/deprotonation process of the amino groups.Based on the different responsive behaviors,ratiometric detection and imaging of L-lysine and pH are achieved.The prepared ratiometric CPDs probe is successfully applied for L-lysine and pH sensing and imaging at two emission channels in live cell and zebrafish with satisfactory results.
