The southern house mosquito, Culex quinquefasciatus, predominantly exists all through the tropics acts as a vector causing several parasitic diseases;it is very important mosquito in transmitting certain diseases whic...The southern house mosquito, Culex quinquefasciatus, predominantly exists all through the tropics acts as a vector causing several parasitic diseases;it is very important mosquito in transmitting certain diseases which include filariasis. The progression of this disease is due to swift urbanization. In fact, morphological identification mosquito species is very difficult task;moreover, origin of the species is unpredictable. Recently, DNA barcoding of partial mitochondrial COI gene is very popular in DNA based identification of mosquito species. Timely reporting of mosquitoes is very crucial in vector born disease control. In this respect, in the present study, larvae of Culex quinquefasciatus species in Hyderabad, India was collected and reared to adults. Total DNA including mitochondrial DNA was isolated and partial mitochondrial COI gene was amplified. Interestingly, Hyderabad species sequence is found to be novel when compared to other geographical regions of the world, therefore, the same sequence was submitted to NCBI-Nucleotide database and accession number of JX08870 was assigned;to confirm it further, multiple alignment of Culex species was accomplished. Further, phylogenetic analysis was carried out to check the evolutionary pattern of Hyderabad species. In conclusion, partial mitochondrial COI gene of Hyderabad species is found to be novel and it can be used as DNA barcode for molecular characterization. Sequence alignment and phylogenetic analysis revealed that Hyderabad species is more close to UK species.展开更多
【目的】探讨COI基因作为标准DNA条形码技术鉴定外形差异较小的地方鸡品种的可行性。【方法】以华南地区9种优质地方鸡(怀乡鸡、清远麻鸡、惠阳胡须鸡、中山沙栏鸡、阳山鸡、杏花鸡、五华三黄鸡、文昌鸡和广西三黄鸡)和国外引进品种隐...【目的】探讨COI基因作为标准DNA条形码技术鉴定外形差异较小的地方鸡品种的可行性。【方法】以华南地区9种优质地方鸡(怀乡鸡、清远麻鸡、惠阳胡须鸡、中山沙栏鸡、阳山鸡、杏花鸡、五华三黄鸡、文昌鸡和广西三黄鸡)和国外引进品种隐性白羽鸡为试验材料,测定标准的DNA条形码技术的线粒体细胞色素C氧化酶亚基I(cytochrome C oxidase subunit I,COI),同时下载已发表的31条家鸡和原鸡及绿头鸭的COI基因序列,分析品种遗传多样性与遗传距离,构建单倍型中介网络图和系统发生邻接树,界定区分品种特异的单倍型。【结果】除去PCR引物序列,获得了695 bp COI基因片段。根据标准的DNA条形码序列,截取648 bp线粒体COI基因序列进行分析。10个鸡品种203个个体共检测到110个变异位点,占分析位点的16.98%,其中90个单一位点突变,20个简约信息位点。平均核苷酸多样性为0.00394(0.00349—0.00560),平均单倍型多样性为0.832(0.763—0.905),其中五华三黄鸡最高,中山沙栏鸡次之,文昌鸡最低。定义了84种单倍型,单倍型1为9个地方鸡种所共享,出现频率为64次;单倍型9和5为家鸡和隐性白羽鸡共享,出现频率分别为29次和19次;每个鸡品种均有品种特异的单倍型。广西三黄鸡、五华三黄鸡与中山沙栏鸡的单倍型数最多,为13个,隐性白羽鸡与清远麻鸡的最少,为8个。不同品种的单倍型分布差异较大,如杏花鸡的单倍型主要分布在1,清远麻鸡主要分布在1和9,惠阳胡须鸡主要分布在1、5和9,隐性白羽鸡主要分布在9和79。10个鸡种品种间遗传距离范围为0.003—0.006,净遗传距离为0—0.003;鸡品种间的遗传距离一般大于鸡品种内的遗传距离;绿头鸭与鸡品种间的遗传距离大于0.2。中介网络图将84个单倍型分为3条进化枝,呈现出一定的品种特异性,如以单倍型9为起点的进化枝没有广西三黄鸡和文昌鸡分布,但另外�展开更多
文摘The southern house mosquito, Culex quinquefasciatus, predominantly exists all through the tropics acts as a vector causing several parasitic diseases;it is very important mosquito in transmitting certain diseases which include filariasis. The progression of this disease is due to swift urbanization. In fact, morphological identification mosquito species is very difficult task;moreover, origin of the species is unpredictable. Recently, DNA barcoding of partial mitochondrial COI gene is very popular in DNA based identification of mosquito species. Timely reporting of mosquitoes is very crucial in vector born disease control. In this respect, in the present study, larvae of Culex quinquefasciatus species in Hyderabad, India was collected and reared to adults. Total DNA including mitochondrial DNA was isolated and partial mitochondrial COI gene was amplified. Interestingly, Hyderabad species sequence is found to be novel when compared to other geographical regions of the world, therefore, the same sequence was submitted to NCBI-Nucleotide database and accession number of JX08870 was assigned;to confirm it further, multiple alignment of Culex species was accomplished. Further, phylogenetic analysis was carried out to check the evolutionary pattern of Hyderabad species. In conclusion, partial mitochondrial COI gene of Hyderabad species is found to be novel and it can be used as DNA barcode for molecular characterization. Sequence alignment and phylogenetic analysis revealed that Hyderabad species is more close to UK species.
文摘【目的】探讨COI基因作为标准DNA条形码技术鉴定外形差异较小的地方鸡品种的可行性。【方法】以华南地区9种优质地方鸡(怀乡鸡、清远麻鸡、惠阳胡须鸡、中山沙栏鸡、阳山鸡、杏花鸡、五华三黄鸡、文昌鸡和广西三黄鸡)和国外引进品种隐性白羽鸡为试验材料,测定标准的DNA条形码技术的线粒体细胞色素C氧化酶亚基I(cytochrome C oxidase subunit I,COI),同时下载已发表的31条家鸡和原鸡及绿头鸭的COI基因序列,分析品种遗传多样性与遗传距离,构建单倍型中介网络图和系统发生邻接树,界定区分品种特异的单倍型。【结果】除去PCR引物序列,获得了695 bp COI基因片段。根据标准的DNA条形码序列,截取648 bp线粒体COI基因序列进行分析。10个鸡品种203个个体共检测到110个变异位点,占分析位点的16.98%,其中90个单一位点突变,20个简约信息位点。平均核苷酸多样性为0.00394(0.00349—0.00560),平均单倍型多样性为0.832(0.763—0.905),其中五华三黄鸡最高,中山沙栏鸡次之,文昌鸡最低。定义了84种单倍型,单倍型1为9个地方鸡种所共享,出现频率为64次;单倍型9和5为家鸡和隐性白羽鸡共享,出现频率分别为29次和19次;每个鸡品种均有品种特异的单倍型。广西三黄鸡、五华三黄鸡与中山沙栏鸡的单倍型数最多,为13个,隐性白羽鸡与清远麻鸡的最少,为8个。不同品种的单倍型分布差异较大,如杏花鸡的单倍型主要分布在1,清远麻鸡主要分布在1和9,惠阳胡须鸡主要分布在1、5和9,隐性白羽鸡主要分布在9和79。10个鸡种品种间遗传距离范围为0.003—0.006,净遗传距离为0—0.003;鸡品种间的遗传距离一般大于鸡品种内的遗传距离;绿头鸭与鸡品种间的遗传距离大于0.2。中介网络图将84个单倍型分为3条进化枝,呈现出一定的品种特异性,如以单倍型9为起点的进化枝没有广西三黄鸡和文昌鸡分布,但另外�
