Object: The authors studied the influence of CO2 pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation. Method: They set up a simulation o...Object: The authors studied the influence of CO2 pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation. Method: They set up a simulation of pneumoperitoneum under different CO2 pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated. Result: When the pressure of CO2 pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5,178.0, 181.6 nmol/(g.min) and PP2a was 4158.3, 4066.9, 3984.0, 3878.5 nmol/(g.min) respectively. After 1 week, the cancer cells concentration was 2.15×105, 2.03×105, 2.20×105, 2.18×105 L-1. Conclusion: CO2 pneumoperitoneum could promote acidosis in cancer cells, inducing the activation of protein kinase C and deactivation of protein phosphatase 2a, but it could not accelerate the mitosis rate of the cancer cells.展开更多
目的探讨妇科腹腔镜手术头低脚高位和CO2气腹对循环的影响。方法选择于北京妇产医院行妇科腹腔镜手术的10例ASAⅠ级的健康女性为研究对象,以瑞芬太尼和依托咪酯静脉诱导气管插管和维持。监测创建气腹前平躺时(T1)、头低10°时(T2)...目的探讨妇科腹腔镜手术头低脚高位和CO2气腹对循环的影响。方法选择于北京妇产医院行妇科腹腔镜手术的10例ASAⅠ级的健康女性为研究对象,以瑞芬太尼和依托咪酯静脉诱导气管插管和维持。监测创建气腹前平躺时(T1)、头低10°时(T2)、头低20°时(T3)、手术开始时、创建气腹后气腹压10 mm Hg及15 mm Hg平躺时、头低10°时及头低20°时(T4-1、T4-2、T4-3和T5-1、T5-2、T5-3)的心率、收缩压(SBP)、舒张压(DBP)、平均动脉压、心排量、心指数、每搏量、每搏指数、每搏量变异和脑电双频指数(BIS)。结果腹腔充气前,仰卧位、头低脚10°和20°三个时点,各项血流动力学参数无明显变化;充气前SBP为(105.8±4.0)mm Hg、DBP为(64.1±2.2)mm Hg,充气后SBP为(121.4±4.1)mm Hg、DBP为(81.3±3.6)mm Hg,充气后血压明显高于充气前,差异有高度统计学意义(P<0.01),但是充气后体位对各项血流动力学参数并无明显影响。气腹压12 mm Hg和15 mm Hg相比,血流动力学无明显改变。结论无合并症的患者可以较好地耐受常规的头低脚高位对机体的影响,腹腔镜下CO2气腹对患者的循环影响较大,如果高气腹压持续时间较长,会对患者造成不良影响,特别是老年人、高血压及心脏病患者。展开更多
基金Project supported by Research and Development Funds of Second Affiliated Hospital, School of Medicine, Zhejiang University, China
文摘Object: The authors studied the influence of CO2 pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation. Method: They set up a simulation of pneumoperitoneum under different CO2 pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated. Result: When the pressure of CO2 pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5,178.0, 181.6 nmol/(g.min) and PP2a was 4158.3, 4066.9, 3984.0, 3878.5 nmol/(g.min) respectively. After 1 week, the cancer cells concentration was 2.15×105, 2.03×105, 2.20×105, 2.18×105 L-1. Conclusion: CO2 pneumoperitoneum could promote acidosis in cancer cells, inducing the activation of protein kinase C and deactivation of protein phosphatase 2a, but it could not accelerate the mitosis rate of the cancer cells.
文摘目的探讨妇科腹腔镜手术头低脚高位和CO2气腹对循环的影响。方法选择于北京妇产医院行妇科腹腔镜手术的10例ASAⅠ级的健康女性为研究对象,以瑞芬太尼和依托咪酯静脉诱导气管插管和维持。监测创建气腹前平躺时(T1)、头低10°时(T2)、头低20°时(T3)、手术开始时、创建气腹后气腹压10 mm Hg及15 mm Hg平躺时、头低10°时及头低20°时(T4-1、T4-2、T4-3和T5-1、T5-2、T5-3)的心率、收缩压(SBP)、舒张压(DBP)、平均动脉压、心排量、心指数、每搏量、每搏指数、每搏量变异和脑电双频指数(BIS)。结果腹腔充气前,仰卧位、头低脚10°和20°三个时点,各项血流动力学参数无明显变化;充气前SBP为(105.8±4.0)mm Hg、DBP为(64.1±2.2)mm Hg,充气后SBP为(121.4±4.1)mm Hg、DBP为(81.3±3.6)mm Hg,充气后血压明显高于充气前,差异有高度统计学意义(P<0.01),但是充气后体位对各项血流动力学参数并无明显影响。气腹压12 mm Hg和15 mm Hg相比,血流动力学无明显改变。结论无合并症的患者可以较好地耐受常规的头低脚高位对机体的影响,腹腔镜下CO2气腹对患者的循环影响较大,如果高气腹压持续时间较长,会对患者造成不良影响,特别是老年人、高血压及心脏病患者。
