Nasopharyngeal carcinoma （NPC） is one of the highly prevalent malignancies in Southeast Asia. Chemotherapy and radiotherapy are commonly administrated to treat nasopharyngeal carcinoma, but these therapies can not p...Nasopharyngeal carcinoma （NPC） is one of the highly prevalent malignancies in Southeast Asia. Chemotherapy and radiotherapy are commonly administrated to treat nasopharyngeal carcinoma, but these therapies can not prevent the recurrence and metastasis of tumor cells. Furthermore, these therapies have severe side effects. Therefore, it is important to find a more effective therapy, e. g., a gene therapy for treatment of nasopharyngeal carcinoma. Cecropin AD （Cad） is a hybrid peptide that exhibits higher antibacterial activity than natural cecropin. It consists of 1-11 amino acid residues in the N-end of cecropin A and 12-37 amino acid residues in the c-end of cecropin D. The role of Cad in the malignant cells remains unclear. In this study, the Cad gene was cloned and transferred into NPC CNE2 cells and its pro-apoptotic effect were investigated.展开更多
目的:研究Ku80沉默对鼻咽癌细胞系放射敏感性和凋亡的影响。方法:q RT-PCR测定鼻咽癌细胞系和正常鼻咽部细胞系中Ku80 m RNA的相对表达水平;将鼻咽癌细胞系CNE2分成对照组、阴性对照Sh RNA组及Ku80 Sh RNA组,分别不感染慢病毒、感染阴性...目的:研究Ku80沉默对鼻咽癌细胞系放射敏感性和凋亡的影响。方法:q RT-PCR测定鼻咽癌细胞系和正常鼻咽部细胞系中Ku80 m RNA的相对表达水平;将鼻咽癌细胞系CNE2分成对照组、阴性对照Sh RNA组及Ku80 Sh RNA组,分别不感染慢病毒、感染阴性Sh RNA慢病毒、感染Ku80 sh RNA慢病毒,测定3组的感染效率,将上述3组细胞分别照射0,2,4,6,8及10 Gy,测定三组细胞存活率;流式细胞术测定细胞凋亡率。结果:Ku80 m RNA在鼻咽癌细胞系CNE2,CNE1,HONE-1及C666中相对表达量分别为9.0±0.9,8.0±0.3,5.0±0.4及4.0±0.2,Ku80 m RNA在正常鼻咽上皮细胞系NP69中的相对表达量为1.0,Ku80 m RNA在鼻咽癌细胞系中的相对表达量显著高于正常鼻咽上皮细胞系NP69,差异有统计学意义(P<0.05)。慢病毒感染后,Ku80 sh RNA组Ku80 m RNA的相对表达量为0.1±0.03,显著低于阴性对照sh RNA组的0.93±0.04,差异有统计学意义(P<0.001);Ku80s h R N A组Ku 8 0蛋白相对表达量为0.1 5±0.0 5,显著低于阴性对照s h R N A组的1.0,差异有统计学意义(P<0.001)。Ku80 sh RNA组的细胞存活率显著低于对照组和阴性对照sh RNA组;照射后48 h,Ku80 Sh RNA组细胞凋亡率为20.0%±0.9%,对照组及阴性对照Sh RNA组凋亡率分别为4.3%±0.8%,5.0%±0.8%,Ku80 Sh RNA组凋亡率显著高于对照组和阴性对照Sh RNA组。结论:Ku80沉默表达可增强鼻咽癌细胞放射敏感性,并诱导细胞凋亡,沉默Ku80表达有望成为鼻咽癌放射增敏的靶点。展开更多
基金This work was supported by a grant from the National 973 Project of China (No. G1999054204).
文摘Nasopharyngeal carcinoma （NPC） is one of the highly prevalent malignancies in Southeast Asia. Chemotherapy and radiotherapy are commonly administrated to treat nasopharyngeal carcinoma, but these therapies can not prevent the recurrence and metastasis of tumor cells. Furthermore, these therapies have severe side effects. Therefore, it is important to find a more effective therapy, e. g., a gene therapy for treatment of nasopharyngeal carcinoma. Cecropin AD （Cad） is a hybrid peptide that exhibits higher antibacterial activity than natural cecropin. It consists of 1-11 amino acid residues in the N-end of cecropin A and 12-37 amino acid residues in the c-end of cecropin D. The role of Cad in the malignant cells remains unclear. In this study, the Cad gene was cloned and transferred into NPC CNE2 cells and its pro-apoptotic effect were investigated.
文摘目的:研究Ku80沉默对鼻咽癌细胞系放射敏感性和凋亡的影响。方法:q RT-PCR测定鼻咽癌细胞系和正常鼻咽部细胞系中Ku80 m RNA的相对表达水平;将鼻咽癌细胞系CNE2分成对照组、阴性对照Sh RNA组及Ku80 Sh RNA组,分别不感染慢病毒、感染阴性Sh RNA慢病毒、感染Ku80 sh RNA慢病毒,测定3组的感染效率,将上述3组细胞分别照射0,2,4,6,8及10 Gy,测定三组细胞存活率;流式细胞术测定细胞凋亡率。结果:Ku80 m RNA在鼻咽癌细胞系CNE2,CNE1,HONE-1及C666中相对表达量分别为9.0±0.9,8.0±0.3,5.0±0.4及4.0±0.2,Ku80 m RNA在正常鼻咽上皮细胞系NP69中的相对表达量为1.0,Ku80 m RNA在鼻咽癌细胞系中的相对表达量显著高于正常鼻咽上皮细胞系NP69,差异有统计学意义(P<0.05)。慢病毒感染后,Ku80 sh RNA组Ku80 m RNA的相对表达量为0.1±0.03,显著低于阴性对照sh RNA组的0.93±0.04,差异有统计学意义(P<0.001);Ku80s h R N A组Ku 8 0蛋白相对表达量为0.1 5±0.0 5,显著低于阴性对照s h R N A组的1.0,差异有统计学意义(P<0.001)。Ku80 sh RNA组的细胞存活率显著低于对照组和阴性对照sh RNA组;照射后48 h,Ku80 Sh RNA组细胞凋亡率为20.0%±0.9%,对照组及阴性对照Sh RNA组凋亡率分别为4.3%±0.8%,5.0%±0.8%,Ku80 Sh RNA组凋亡率显著高于对照组和阴性对照Sh RNA组。结论:Ku80沉默表达可增强鼻咽癌细胞放射敏感性,并诱导细胞凋亡,沉默Ku80表达有望成为鼻咽癌放射增敏的靶点。
