AIM: To assess whether the polymorphisms of NOD2/ CARD15 , autophagy-related 16-like 1 (ATG16L1 ), and interleukin-23 receptor (IL23R ) genes play a more critical role in the susceptibility of childhood-onset than in ...AIM: To assess whether the polymorphisms of NOD2/ CARD15 , autophagy-related 16-like 1 (ATG16L1 ), and interleukin-23 receptor (IL23R ) genes play a more critical role in the susceptibility of childhood-onset than in adult-onset Crohn’s disease (CD). METHODS: Polymorphisms R702W, G908R, and 3020insC of NOD2/CARD15 ; rs2241880 A/G of ATG16L1 , and rs11209026 (R381Q) of IL23R gene were assessed in 110 childhood-onset CD, 364 adult-onset CD, and 539 healthy individuals. Analysis of polymorphisms R702W, G908R, and 3020insC of NOD2/CARD15 genotyping was performed by allele specific polymerase chain reaction (PCR) or by PCR-restriction fragment length polymor-phism analysis. The polymorphisms rs2241880 A/G of the ATG16L1 , and rs11209026 (R381Q) of the IL23R gene in the children’s cohort were genotyped by PCR and melting curve analysis whereas adult group genotyping was performed using the Affymetrix Genome-Wide Human SNP Array 5.0 (500K). RESULTS: The 3020insC allele in NOD2/CARD15 was significantly higher in childhood than in adult-onset CD (P = 0.0067). Association with at least 1 NOD2/CARD15 variant was specific for ileal disease (with or without co- lonic involvement). Even if the frequency of G allele of the rs2241880 ATG16L1 polymorphism was increased in both paediatric and adult CD patients compared to con- trols (P = 0.017 and P = 0.001, respectively), no difference was observed between the childhood and the adult cohort. The rare Q allele of IL23R rs11209026 polymorphism was underrepresented in both paediatric and adult CD cases (P = 0.0018 and P = 0.04, respectively) and no difference was observed between the childhood and the adult cohort. The presence of the rs2241880 ATG16L1 and rs11209026 IL23R polymorphisms did not influence disease phenotype. CONCLUSION: Polymorphism 3020insC in NOD2/ CARD15 occurs statistically significantly more often in patients with childhood-onset CD than in patients with adult-onset CD. The ATG16L1 and IL23R variants are associated with susceptibility to CD, but not earlyonse展开更多
系统性红斑狼疮(SLE)是一种以多系统损害和血清中出现自身抗体为特征的自身免疫性疾病。20多年来,其分类标准经历了不同专业协会或团体的几轮修改,每个标准具有不同的敏感性和特异性,为了更好地提高临床诊断的特异性和敏感性,欧洲抗风...系统性红斑狼疮(SLE)是一种以多系统损害和血清中出现自身抗体为特征的自身免疫性疾病。20多年来,其分类标准经历了不同专业协会或团体的几轮修改,每个标准具有不同的敏感性和特异性,为了更好地提高临床诊断的特异性和敏感性,欧洲抗风湿病联盟(European League Against Rheumatism,EULAR)联合美国风湿病学会(ACR)于2019年发布了新的SLE分类系统。文章将结合笔者的理解简要介绍该标准的应用。展开更多
We present a retrospective review of DNA immunoadsorption (DNA-IA) therapy on clinical symptoms as well as indicators in pediatric cases with systemic lupus erythematosus (SLE), and follow up the short-term curative e...We present a retrospective review of DNA immunoadsorption (DNA-IA) therapy on clinical symptoms as well as indicators in pediatric cases with systemic lupus erythematosus (SLE), and follow up the short-term curative effects. 16 SLE cases were treated by DNA-IA for 3 times every other day. We observed the changes on clinical manifestations and immunological indicators, in order to compare the alteration of these indicators including clinical manifestations, Systemic Lupus Erythematosus Disease Active Index (SLEDAI) scores, 24 hurinary protein excretion, autoantibodies, serum IgG and complement C3. 13 cases were followed up regularly, within 3 months after DNA-IA therapy, 12 cases of clinical manifestations improved (92.3%). SLEDAI scores in 10 cases decreased from (16.20 ± 12.54) to less than 5 (76.9%), 8 cases of ANA, anti-DNA antibodies were negative (61.5%), 13 cases with IgG level in serum recovered to normal (10.39 ± 4.38) g/L, C3 level rose to normal (1.06 ± 0.23) g/L. 3 to 6 months after IA, clinical manifestations and laboratory examinations in all cases got maximum improved. 9 months after IA, SLEDAI score in 2 cases (15.4%) rose to more than 5, anti-DNA antibody in 2 cases (15.4%) became positive, and 1case (7.7%) with serum C3 decreased again. 2 cases died from multiple organs dysfunction within 3 to 6 months after IA. No serious complications were found during DNA-IA. We recommend that DNA immunoadsorption is a safe and effective therapy for active childhood-onset SLE, which could improve clinical symptoms, eliminate ANA and anti-DNA antibodies. Combining with corticosteroids and immunosuppressive drugs, DNA-IA could significantly reduce the activity of disease and protect vital organs function in the short term.展开更多
Background:The lack of social support for adults with epilepsy(AWEs)is receiving increased attention,as it may result in low quality of life.This study was aimed to confirm the demographic characteristics of and clini...Background:The lack of social support for adults with epilepsy(AWEs)is receiving increased attention,as it may result in low quality of life.This study was aimed to confirm the demographic characteristics of and clinical factors associated with social support for AWEs.Methods:AWEs were consecutively recruited from our hospital.The 10-term Social Support Rating Scale(SSRS)was used to measure social support.A linear regression analysis with stepwise selection was performed to analyze the independent variables associated with social support for AWEs.Results:In total,165 AWEs were consecutively included in the present study.Linear regression analysis showed that the marital status(t=-3.550,β=-0.272,P=0.001),the age at onset(t=2.545,β=0.192,P=0.012),and the QOLIE-31 score(t=3.144,β=0.221,P=0.002)were independent variables associated with social support for AWEs.Conclusions:Our findings suggest that the poor social support is associated with childhood onset of epilepsy and the unmarried status.This study also confirmed a negative influence of low social support on quality of life in AWEs.展开更多
目的评价贝利尤单抗治疗儿童系统性红斑狼疮(cSLE)的有效性和安全性,为临床用药提供循证参考。方法计算机检索中国知网、万方数据、维普网、SinoMed、PubMed、Embase、Web of Science、Cochrane图书馆,收集贝利尤单抗或贝利尤单抗联合...目的评价贝利尤单抗治疗儿童系统性红斑狼疮(cSLE)的有效性和安全性,为临床用药提供循证参考。方法计算机检索中国知网、万方数据、维普网、SinoMed、PubMed、Embase、Web of Science、Cochrane图书馆,收集贝利尤单抗或贝利尤单抗联合激素或贝利尤单抗联合激素和传统药物(试验组)对比安慰剂或激素或传统药物或传统药物联合激素等基础治疗(对照组)的随机对照试验(RCT),检索时限均为建库至2023年4月9日。筛选文献、提取资料后,采用Cochrane系统评价员手册5.1.0推荐的偏倚风险评估工具对纳入文献进行质量评价,采用RevMan 5.4软件进行Meta分析、敏感性分析。结果纳入7项RCT,共计510例患儿。Meta分析结果显示,试验组患儿的临床有效率显著优于对照组[OR=6.16,95%CI(2.23,17.00),P=0.0004],两组患儿的系统性红斑狼疮活动指数[MD=-1.73,95%CI(-3.50,0.05),P=0.06]、不良反应发生率[OR=0.72,95%CI(0.43,1.19),P=0.02]、补体C3水平[MD=0.12,95%CI(-0.06,0.30),P=0.18]、补体C4水平[MD=0.08,95%CI(-0.07,0.24),P=0.30]、系统性红斑狼疮反应指数4反应率[OR=1.52,95%CI(0.94,2.44),P=0.09]比较,差异均无统计学意义。敏感性分析结果显示,以系统性红斑狼疮活动指数、补体C3水平、补体C4水平为指标时,本研究所得结果稳健。结论贝利尤单抗治疗cSLE的疗效较好,且安全性与基础治疗相当。展开更多
文摘AIM: To assess whether the polymorphisms of NOD2/ CARD15 , autophagy-related 16-like 1 (ATG16L1 ), and interleukin-23 receptor (IL23R ) genes play a more critical role in the susceptibility of childhood-onset than in adult-onset Crohn’s disease (CD). METHODS: Polymorphisms R702W, G908R, and 3020insC of NOD2/CARD15 ; rs2241880 A/G of ATG16L1 , and rs11209026 (R381Q) of IL23R gene were assessed in 110 childhood-onset CD, 364 adult-onset CD, and 539 healthy individuals. Analysis of polymorphisms R702W, G908R, and 3020insC of NOD2/CARD15 genotyping was performed by allele specific polymerase chain reaction (PCR) or by PCR-restriction fragment length polymor-phism analysis. The polymorphisms rs2241880 A/G of the ATG16L1 , and rs11209026 (R381Q) of the IL23R gene in the children’s cohort were genotyped by PCR and melting curve analysis whereas adult group genotyping was performed using the Affymetrix Genome-Wide Human SNP Array 5.0 (500K). RESULTS: The 3020insC allele in NOD2/CARD15 was significantly higher in childhood than in adult-onset CD (P = 0.0067). Association with at least 1 NOD2/CARD15 variant was specific for ileal disease (with or without co- lonic involvement). Even if the frequency of G allele of the rs2241880 ATG16L1 polymorphism was increased in both paediatric and adult CD patients compared to con- trols (P = 0.017 and P = 0.001, respectively), no difference was observed between the childhood and the adult cohort. The rare Q allele of IL23R rs11209026 polymorphism was underrepresented in both paediatric and adult CD cases (P = 0.0018 and P = 0.04, respectively) and no difference was observed between the childhood and the adult cohort. The presence of the rs2241880 ATG16L1 and rs11209026 IL23R polymorphisms did not influence disease phenotype. CONCLUSION: Polymorphism 3020insC in NOD2/ CARD15 occurs statistically significantly more often in patients with childhood-onset CD than in patients with adult-onset CD. The ATG16L1 and IL23R variants are associated with susceptibility to CD, but not earlyonse
文摘系统性红斑狼疮(SLE)是一种以多系统损害和血清中出现自身抗体为特征的自身免疫性疾病。20多年来,其分类标准经历了不同专业协会或团体的几轮修改,每个标准具有不同的敏感性和特异性,为了更好地提高临床诊断的特异性和敏感性,欧洲抗风湿病联盟(European League Against Rheumatism,EULAR)联合美国风湿病学会(ACR)于2019年发布了新的SLE分类系统。文章将结合笔者的理解简要介绍该标准的应用。
文摘We present a retrospective review of DNA immunoadsorption (DNA-IA) therapy on clinical symptoms as well as indicators in pediatric cases with systemic lupus erythematosus (SLE), and follow up the short-term curative effects. 16 SLE cases were treated by DNA-IA for 3 times every other day. We observed the changes on clinical manifestations and immunological indicators, in order to compare the alteration of these indicators including clinical manifestations, Systemic Lupus Erythematosus Disease Active Index (SLEDAI) scores, 24 hurinary protein excretion, autoantibodies, serum IgG and complement C3. 13 cases were followed up regularly, within 3 months after DNA-IA therapy, 12 cases of clinical manifestations improved (92.3%). SLEDAI scores in 10 cases decreased from (16.20 ± 12.54) to less than 5 (76.9%), 8 cases of ANA, anti-DNA antibodies were negative (61.5%), 13 cases with IgG level in serum recovered to normal (10.39 ± 4.38) g/L, C3 level rose to normal (1.06 ± 0.23) g/L. 3 to 6 months after IA, clinical manifestations and laboratory examinations in all cases got maximum improved. 9 months after IA, SLEDAI score in 2 cases (15.4%) rose to more than 5, anti-DNA antibody in 2 cases (15.4%) became positive, and 1case (7.7%) with serum C3 decreased again. 2 cases died from multiple organs dysfunction within 3 to 6 months after IA. No serious complications were found during DNA-IA. We recommend that DNA immunoadsorption is a safe and effective therapy for active childhood-onset SLE, which could improve clinical symptoms, eliminate ANA and anti-DNA antibodies. Combining with corticosteroids and immunosuppressive drugs, DNA-IA could significantly reduce the activity of disease and protect vital organs function in the short term.
基金The present study was funded by the Jilin University First Hospital Clinical Cultivation Fund Program(LCPYJJ2017006).
文摘Background:The lack of social support for adults with epilepsy(AWEs)is receiving increased attention,as it may result in low quality of life.This study was aimed to confirm the demographic characteristics of and clinical factors associated with social support for AWEs.Methods:AWEs were consecutively recruited from our hospital.The 10-term Social Support Rating Scale(SSRS)was used to measure social support.A linear regression analysis with stepwise selection was performed to analyze the independent variables associated with social support for AWEs.Results:In total,165 AWEs were consecutively included in the present study.Linear regression analysis showed that the marital status(t=-3.550,β=-0.272,P=0.001),the age at onset(t=2.545,β=0.192,P=0.012),and the QOLIE-31 score(t=3.144,β=0.221,P=0.002)were independent variables associated with social support for AWEs.Conclusions:Our findings suggest that the poor social support is associated with childhood onset of epilepsy and the unmarried status.This study also confirmed a negative influence of low social support on quality of life in AWEs.
文摘目的评价贝利尤单抗治疗儿童系统性红斑狼疮(cSLE)的有效性和安全性,为临床用药提供循证参考。方法计算机检索中国知网、万方数据、维普网、SinoMed、PubMed、Embase、Web of Science、Cochrane图书馆,收集贝利尤单抗或贝利尤单抗联合激素或贝利尤单抗联合激素和传统药物(试验组)对比安慰剂或激素或传统药物或传统药物联合激素等基础治疗(对照组)的随机对照试验(RCT),检索时限均为建库至2023年4月9日。筛选文献、提取资料后,采用Cochrane系统评价员手册5.1.0推荐的偏倚风险评估工具对纳入文献进行质量评价,采用RevMan 5.4软件进行Meta分析、敏感性分析。结果纳入7项RCT,共计510例患儿。Meta分析结果显示,试验组患儿的临床有效率显著优于对照组[OR=6.16,95%CI(2.23,17.00),P=0.0004],两组患儿的系统性红斑狼疮活动指数[MD=-1.73,95%CI(-3.50,0.05),P=0.06]、不良反应发生率[OR=0.72,95%CI(0.43,1.19),P=0.02]、补体C3水平[MD=0.12,95%CI(-0.06,0.30),P=0.18]、补体C4水平[MD=0.08,95%CI(-0.07,0.24),P=0.30]、系统性红斑狼疮反应指数4反应率[OR=1.52,95%CI(0.94,2.44),P=0.09]比较,差异均无统计学意义。敏感性分析结果显示,以系统性红斑狼疮活动指数、补体C3水平、补体C4水平为指标时,本研究所得结果稳健。结论贝利尤单抗治疗cSLE的疗效较好,且安全性与基础治疗相当。
