[Objective] The present study was conducted to evaluate the cold resis-tance of four cherry cultivars under different chil ing stresses. [Method] Four cultivars of Cerasus, Early-mahapphala, Red light, Lapins and Kash...[Objective] The present study was conducted to evaluate the cold resis-tance of four cherry cultivars under different chil ing stresses. [Method] Four cultivars of Cerasus, Early-mahapphala, Red light, Lapins and Kashgar Kukan were selected as the experimental materials, and the changes of the relative electric conductivity （REC）, proline （PRO） content, malondialdehyde （MDA） content, superoxide dismu-tase （SOD） activity and peroxidase （POD） activity of dormant branches of the four cherry cultivars under low temperature stress were investigated. [Result] The REC of the four cherry cultivars was Kashgar kukan 〉 Red light 〉 Early-mahapphala 〉Lapins, while the MDA content was Kashgar kukan 〉 Early-mahapphala 〉 Red light 〉Lapins, both showed a rising trend in the four cultivars with the aggravating of the low temperature stress. The PRO content, POD and SOD activities of al cultivars were precisely contrary to their REC and MDA content, as al of them exhibited a sequence as Lapins 〉 Red light 〉 Early-mahapphala 〉 Kashgar kukan in al culti-vars, and got a trend of first rising and then declining. [Conclusion] Therefore, our final evaluation of cold resistance of the four cherry cultivars was Lapins 〉 Red light 〉 Early-mahapphala 〉 Kashgar kukan.展开更多
体育素养(Physical Literacy)是当今全球体育教育研究的热点议题,其测量与评价也是近年来国内、外学者研究的重要内容。加拿大体育素养测评(The Canadian Assessment of Physical Literacy,CAPL)是目前国际应用较为广泛的测评工具,其理...体育素养(Physical Literacy)是当今全球体育教育研究的热点议题,其测量与评价也是近年来国内、外学者研究的重要内容。加拿大体育素养测评(The Canadian Assessment of Physical Literacy,CAPL)是目前国际应用较为广泛的测评工具,其理论模型、测试指标、评分体系都经过相对严密的设计和论证,在国际范围内有一定的影响力。借鉴CAPL的研制和应用,我国体育素养测评的研究在3方面需要提高:1)完善研制程序;2)系统论证测评指标;3)对体育素养总评指标的研究。研究通过介绍CAPL的发展情况,进而分析其对我国体育素养测评方面的启示。展开更多
This study aims to produce an effective subunit vaccine against infectious bursal disease virus （IBDV）. The genes of chicken interleukin-2 （ChIL-2） and IBDV viral protein 2 （VP2） were amplified and fused by spli...This study aims to produce an effective subunit vaccine against infectious bursal disease virus （IBDV）. The genes of chicken interleukin-2 （ChIL-2） and IBDV viral protein 2 （VP2） were amplified and fused by splice overlap extension-polymerase chain reaction （SOE-PCR）. The fusion gene was digested by EcoR I/Kpn I and inserted into pBacPAK8 vector, resulting in recombinant transfer plasmid pBacPakVP2-IL2. The recombinant plasmid was transfected into Sf-9 cells accompanied with hybrid nuclear polyhedrosis virus （HyNPV） genome DNA and lipofectin. Plaque-purification indicated that we had got the recombinant Hy-VP2-IL2. Fusion protein VP2-IL2 was expressed effectively both in insect cells and bombyx mori. The expression of fusion protein was confirmed by ELISA, SDS-PAGE and Western blotting assay, respectively. This efficient system allows us to meet the need for inexpensive vaccines required by the poultry industry. Cellular ＆ Molecular Immunology. 2005;2（3）：231-235.展开更多
目的评价黄芪颗粒治疗小儿病毒性心肌炎的临床效果与安全性。方法检索EMBASE、PUBMED、MEDLINE、AMED、CINAHL、Cochrane图书馆、中国知网数据库、万方数据库、重庆维普中文科技期刊数据库、中国生物医学文献数据库和中国中医药期刊文...目的评价黄芪颗粒治疗小儿病毒性心肌炎的临床效果与安全性。方法检索EMBASE、PUBMED、MEDLINE、AMED、CINAHL、Cochrane图书馆、中国知网数据库、万方数据库、重庆维普中文科技期刊数据库、中国生物医学文献数据库和中国中医药期刊文献数据库从建库至2014年8月15日黄芪颗粒治疗小儿病毒性心肌炎随机对照试验研究,筛选合格研究。应用Cochrane评价员手册进行偏倚风险评价,Jadad评分法进行质量评价。应用Rev Man 5.3软件进行Meta分析。结果符合纳入标准的文献共5篇。Meta分析结果显示,黄芪颗粒治疗小儿病毒性心肌炎治疗组与对照组比较,总有效率比值比(OR)为3.85,95%可信区间(CI)(2.24,6.63),差异有统计学意义(P<0.000 01)。治愈率OR为2.35,95%CI(1.09,5.10),差异有统计学意义(P=0.03)。治疗后心肌酶谱指标(肌酸激酶同工酶、乳酸脱氢酶、谷草转氨酶)治疗组低于对照组,差异有统计学意义。纳入研究未报道有不良反应发生。结论黄芪颗粒治疗小儿病毒性心肌炎临床效果显著,临床安全性较好。但由于本系统评价纳入研究的总体质量较低,研究的数量和样本量均较小,尚需要更多高质量、多中心、大样本、严格设计的随机对试验来进一步验证其疗效与安全性。展开更多
基金Supported by the Science and Technology Supporting Project in Xinjiang Uygur Autonomous Region(201291123)~~
文摘[Objective] The present study was conducted to evaluate the cold resis-tance of four cherry cultivars under different chil ing stresses. [Method] Four cultivars of Cerasus, Early-mahapphala, Red light, Lapins and Kashgar Kukan were selected as the experimental materials, and the changes of the relative electric conductivity （REC）, proline （PRO） content, malondialdehyde （MDA） content, superoxide dismu-tase （SOD） activity and peroxidase （POD） activity of dormant branches of the four cherry cultivars under low temperature stress were investigated. [Result] The REC of the four cherry cultivars was Kashgar kukan 〉 Red light 〉 Early-mahapphala 〉Lapins, while the MDA content was Kashgar kukan 〉 Early-mahapphala 〉 Red light 〉Lapins, both showed a rising trend in the four cultivars with the aggravating of the low temperature stress. The PRO content, POD and SOD activities of al cultivars were precisely contrary to their REC and MDA content, as al of them exhibited a sequence as Lapins 〉 Red light 〉 Early-mahapphala 〉 Kashgar kukan in al culti-vars, and got a trend of first rising and then declining. [Conclusion] Therefore, our final evaluation of cold resistance of the four cherry cultivars was Lapins 〉 Red light 〉 Early-mahapphala 〉 Kashgar kukan.
文摘体育素养(Physical Literacy)是当今全球体育教育研究的热点议题,其测量与评价也是近年来国内、外学者研究的重要内容。加拿大体育素养测评(The Canadian Assessment of Physical Literacy,CAPL)是目前国际应用较为广泛的测评工具,其理论模型、测试指标、评分体系都经过相对严密的设计和论证,在国际范围内有一定的影响力。借鉴CAPL的研制和应用,我国体育素养测评的研究在3方面需要提高:1)完善研制程序;2)系统论证测评指标;3)对体育素养总评指标的研究。研究通过介绍CAPL的发展情况,进而分析其对我国体育素养测评方面的启示。
基金This work was supported in part by the grants from the National High Technology Research and Development Program of China(No.2004BA757C)the Key Project of Zhejiang Province(NO.011102465).
文摘This study aims to produce an effective subunit vaccine against infectious bursal disease virus （IBDV）. The genes of chicken interleukin-2 （ChIL-2） and IBDV viral protein 2 （VP2） were amplified and fused by splice overlap extension-polymerase chain reaction （SOE-PCR）. The fusion gene was digested by EcoR I/Kpn I and inserted into pBacPAK8 vector, resulting in recombinant transfer plasmid pBacPakVP2-IL2. The recombinant plasmid was transfected into Sf-9 cells accompanied with hybrid nuclear polyhedrosis virus （HyNPV） genome DNA and lipofectin. Plaque-purification indicated that we had got the recombinant Hy-VP2-IL2. Fusion protein VP2-IL2 was expressed effectively both in insect cells and bombyx mori. The expression of fusion protein was confirmed by ELISA, SDS-PAGE and Western blotting assay, respectively. This efficient system allows us to meet the need for inexpensive vaccines required by the poultry industry. Cellular ＆ Molecular Immunology. 2005;2（3）：231-235.
文摘目的评价黄芪颗粒治疗小儿病毒性心肌炎的临床效果与安全性。方法检索EMBASE、PUBMED、MEDLINE、AMED、CINAHL、Cochrane图书馆、中国知网数据库、万方数据库、重庆维普中文科技期刊数据库、中国生物医学文献数据库和中国中医药期刊文献数据库从建库至2014年8月15日黄芪颗粒治疗小儿病毒性心肌炎随机对照试验研究,筛选合格研究。应用Cochrane评价员手册进行偏倚风险评价,Jadad评分法进行质量评价。应用Rev Man 5.3软件进行Meta分析。结果符合纳入标准的文献共5篇。Meta分析结果显示,黄芪颗粒治疗小儿病毒性心肌炎治疗组与对照组比较,总有效率比值比(OR)为3.85,95%可信区间(CI)(2.24,6.63),差异有统计学意义(P<0.000 01)。治愈率OR为2.35,95%CI(1.09,5.10),差异有统计学意义(P=0.03)。治疗后心肌酶谱指标(肌酸激酶同工酶、乳酸脱氢酶、谷草转氨酶)治疗组低于对照组,差异有统计学意义。纳入研究未报道有不良反应发生。结论黄芪颗粒治疗小儿病毒性心肌炎临床效果显著,临床安全性较好。但由于本系统评价纳入研究的总体质量较低,研究的数量和样本量均较小,尚需要更多高质量、多中心、大样本、严格设计的随机对试验来进一步验证其疗效与安全性。
