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The Grape Component Resveratrol Interferes with the Function of Chemoattractant Receptors on Phagocytic Leukocytes 被引量:6
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作者 HengyiTao ChunfuWu +6 位作者 YeZhou WanghuaGong XiaZhang PabloIribarren YuqingZhao YingyingLe JimingWang 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2004年第1期50-56,共7页
Resveratrol (3, 5, 4'-trihydroxystilbene) (RV) is a constituent of grape seeds with anti-inflammatory andanti-oxidant activities. In this study, we examined the capacity of RV to modulate the function of Gprotein-... Resveratrol (3, 5, 4'-trihydroxystilbene) (RV) is a constituent of grape seeds with anti-inflammatory andanti-oxidant activities. In this study, we examined the capacity of RV to modulate the function of Gprotein-coupled chemoattractant receptors, which play important roles in inflammation and immune responses.RV, over a non-cytotoxic concentration range, inhibited chemotactic and calcium mobilization responses ofphagocytic cells to selected chemoattractants. At low micromolar concentrations,RV potently reducedsuperoxide anion production by phagocytic leukocytes in response to the bacterial chemotactic peptide fMLF, ahigh affinity ligand for formylpeptide receptor FPR, and Ap.4z, an Alzheimer's disease-associated peptide and aligand for the FPR variant FPRL1. In addition, RV reduced phosphorylation of extracellular signal-regulatedkinase (ERK1/2) and the activation of nuclear factor NF-KB induced by formylpeptide receptor agonists. Theseresults suggest that the inhibition of the function of chemoattractant receptors may contribute to theanti-inflammatory properties of RV Thus, RV may be therapeutically promising for diseases in which activationof formylpeptide receptors contributes to the pathogenic processes. Cellular&Molecular Immunology. 2004;1(1):50-56. 展开更多
关键词 RESVERATROL chemoattractants RECEPTORS sianalin INFLAMMATION
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Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ 被引量:7
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作者 Xuguang Li Yuankun Dai +1 位作者 Tao Shen Changyou Gao 《Regenerative Biomaterials》 SCIE 2017年第3期139-148,共10页
Cell migration in scaffolds plays a crucial role in tissue regeneration,which can better mimic cell behaviors in vivo.In this study,a novel model has been proposed on controlling 3D cell migration in porous collagen-c... Cell migration in scaffolds plays a crucial role in tissue regeneration,which can better mimic cell behaviors in vivo.In this study,a novel model has been proposed on controlling 3D cell migration in porous collagen-chitosan scaffolds with various pore structures under the stimulation of inflammatory cells to mimic the angiogenesis process.Endothelial cells(ECs)cultured atop the scaffolds in the Transwell molds which were placed into a well of a 24-well culture plate were promoted to migrate into the scaffolds by chemoattractants such as vascular endothelial growth factor(VEGF)and tumor necrosis factor-alpha(TNF-α)secreted by the pro-inflammatory macrophages incubated in the well culture plate.The phenotype of macrophages was mediated by 50 ng/ml interferongamma(IFN-c)and different concentrations of lipopolysaccharide(LPS,150–300 ng/ml).The cell migration depth had a positive correlation with LPS concentration,and thereby the TNF-a concentration.The ECs migrated easier to a deeper zone of the scaffolds prepared at10C(187 lm in pore diameter)than that at20C(108 lm in pore diameter)as well.The method provides a useful strategy to study the 3D cell migration,and is helpful to reveal the vascularization process during wound healing in the long run. 展开更多
关键词 cell migration chemoattractants endothelial cells MACROPHAGES scaffolds
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InterInterleukin (IL)-4 Induces Production of Cytokine-induced Neutrophil Chemoattractants (CINCs) and Intercellular Adhesion Molecule (ICAM)-1 in Lungs of Asthmatic Rats 被引量:1
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作者 郭亚丽 黄宏 +3 位作者 曾大雄 赵建平 方慧娟 Jean-pierre Lavoie 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2013年第4期470-478,共9页
Summary: The present study aimed to examine the effect of intedeukin (IL)-4 on neutrophil chemo- taxis in airway inflammation in asthmatic rats and the possible mechanism. Male Wistar rats were intranasally instill... Summary: The present study aimed to examine the effect of intedeukin (IL)-4 on neutrophil chemo- taxis in airway inflammation in asthmatic rats and the possible mechanism. Male Wistar rats were intranasally instilled with recombinant rat (rr) IL-4 (rrlL-4) at different doses [2, 4 or 8μ g/animal, dis- solved in 200 μL normal saline (NS)] or rrlL-4 at 4 μg/animal (dissolved in 200 μL NS). NS (200 μL) and LPS (6 mg/kg/animal, dissolved in 200 IxL NS) were intranasally given respectively in the negative and positive control groups. Moreover, the asthmatic lung inflammation was induced in rats which were then intranasally treated with rrlL-4 (4 μg/animal) or LPS (6 mg/kg/animal). The normal rats treated with different doses of rrlL-4 and those asthmatic rats were sacrificed 6 h later. And animals instilled with rrlL-4 at 4 μg were sacrificed 6, 12 or 24 h later. The bronchoalveolar lavage fluid (BALF) and lungs were harvested for detection of leukocyte counts by Wright-Giemsa staining and lung histopa- thology by haematoxylin-eosin (HE) staining. The levels of cytokine-induced neutrophil chemoattrac- tant (CINC)-I and intercellular adhesion molecule (ICAM)-I in BALF were determined by ELISA. Real-time PCR was used to measure the mRNA expression of C1NCs (CINC-1, CINC-2u, CINC-2a, CINC-3) and ICAM-1 in lung tissues. The results showed that the intranasal instillation of IL-4 did not induce a recruitment of neutrophils in BALF in rats. However, IL-4 could increase the CINC-1 level in BALF in a dose-dependent manner at 6 h. But the mRNA expression levels of CINC-1, C1NC-2a, CINC-2, CINC-3 were not significantly increased in lungs of IL-4-treated rats relative to NS negative control group. Moreover, IL-4 was found to augment the mRNA expression oflCAM-1 in lungs and the ICAM-1 level in BALF at 6 h. However, the increase in CINC-1 and ICAM-1 levels in BALF of IL-4-treated asthmatic rats was not significantly different from that in untreated asthmatic rats. T 展开更多
关键词 INTERLEUKIN-4 cytkine-induced neutrophil chemoattractants intercellular adhesion mole-cule- 1 rat
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根结线虫趋化性研究进展 被引量:3
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作者 王帅 魏钰洋 +3 位作者 张羲 谢佳 胡展 孙然锋 《农药学学报》 CAS CSCD 北大核心 2022年第5期982-996,共15页
植物寄主根部及其根际微生物等释放的化学信号物质在根结线虫寻找寄主、配偶及逃避危险等行为中起着重要的作用。目前,明确根结线虫预侵染阶段的化学信号物质以及相关的分子靶标,以期开发得到植物源和微生物源引诱剂及驱避剂,是国际线... 植物寄主根部及其根际微生物等释放的化学信号物质在根结线虫寻找寄主、配偶及逃避危险等行为中起着重要的作用。目前,明确根结线虫预侵染阶段的化学信号物质以及相关的分子靶标,以期开发得到植物源和微生物源引诱剂及驱避剂,是国际线虫学研究领域的前沿和热点。本文重点综述了根结线虫的趋化性及化学信号物质,概括了线虫诱饵效应与诱捕现象的研究进展,概述了平面琼脂及其改进模型、Pluronic凝胶模型以及沙土/土壤模型在根结线虫趋化性测试中的应用,讨论了根结线虫趋化性研究的意义和难度,并对未来根结线虫趋化性研究的主要方向及前景进行了总结和展望。 展开更多
关键词 根结线虫 趋化性 化学信号物质 诱捕现象 趋化性模型 引诱剂 驱避剂
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Chemoattractants and cytokines in primary ciliary dyskinesia and cystic fibrosis: key players in chronic respiratory diseases 被引量:3
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作者 Maaike Cockx Mieke Gouwy +1 位作者 Jo Van Damme Sofie Struyf 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2018年第4期312-323,共12页
Patients with primary ciliary dyskinesia(PCD)and cystic fibrosis(CF),two inherited disorders,suffer from recurrent airway infections characterized by persistent bacterial colonization and uncontrollable inflammation.A... Patients with primary ciliary dyskinesia(PCD)and cystic fibrosis(CF),two inherited disorders,suffer from recurrent airway infections characterized by persistent bacterial colonization and uncontrollable inflammation.Although present in high counts,neutrophils fail to clear infection in the airways.High levels of C-X-C motif chemokine ligand 8/interleukin-8(CXCL8/IL-8),the most potent chemokine to attract neutrophils to sites of infection,are detected in the sputum of both patient groups and might cause the high neutrophil influx in the airways.Furthermore,in CF,airway neutrophils are highly activated because of the genetic defect and the high levels of proinflammatory chemoattractants and cytokines(e.g.,CXCL8/IL-8,tumor necrosis factor-αand IL-17).The overactive state of neutrophils leads to lung damage and fuels the vicious circle of infection,excessive inflammation and tissue damage.The inflammatory process in CF airways is well characterized,whereas the lung pathology in PCD is far less studied.The knowledge of CF lung pathology could be useful to guide molecular investigations of the inflammatory processes in PCD lungs.Current available therapies can not completely remedy the chronic airway infections in these diseases.This review gives an overview of the role that chemoattractants and cytokines play in these neutrophil-dominated lung pathologies.Finally,the most frequently applied treatments in CF and PCD and new experimental therapies to reduce neutrophil-dominated airway inflammation are described. 展开更多
关键词 chemoattractants cystic fibrosis CYTOKINES primary ciliary dyskinesia
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鞘内注射氯胺酮对镜像痛大鼠热痛敏的影响及可能机制 被引量:2
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作者 黄东 程绍波 +2 位作者 谷永红 阎雪彬 王明安 《中国疼痛医学杂志》 CAS CSCD 北大核心 2007年第4期220-223,共4页
目的:探讨氯胺酮鞘内注射对镜像痛大鼠热痛敏的影响及可能机制。方法:30只雄性SD大鼠建立大鼠坐骨神经镜像痛模型,随机分为3组(n=10):Ⅰ组鞘内注射生理盐水,Ⅱ组鞘内注射氯胺酮25μg,Ⅲ组鞘内注射氯胺酮50μg,分别测定鞘内给药前(T1)、... 目的:探讨氯胺酮鞘内注射对镜像痛大鼠热痛敏的影响及可能机制。方法:30只雄性SD大鼠建立大鼠坐骨神经镜像痛模型,随机分为3组(n=10):Ⅰ组鞘内注射生理盐水,Ⅱ组鞘内注射氯胺酮25μg,Ⅲ组鞘内注射氯胺酮50μg,分别测定鞘内给药前(T1)、给药后2h(T2)、4h(T3)、6h(T4)的大鼠热缩足反射潜伏期(TWL)、运动功能。每组大鼠在给药6h后处死,取出腰段脊髓背角,石蜡切片,采用免疫组化检测单核细胞趋化蛋白-1(MCP-1)的表达。结果:鞘内给药后,Ⅱ组、Ⅲ组在各时间点与Ⅰ组及给药前相比,两侧TWL明显延长,比较有显著差异(P<0.01)。Ⅰ组MCP-1平均积分光密度值(IOD)表达明显高于Ⅱ组和Ⅲ组(P<0.01),鞘内注射氯胺酮后大鼠运动功能不受明显影响。结论:鞘内注射氯胺酮可抑制镜像痛大鼠的热痛觉过敏,该作用可能与抑制脊髓背角的MCP-1表达有关。 展开更多
关键词 氯胺酮 镜像痛 鞘内注射 单核细胞趋化蛋白-1
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Role of the renin angiotensin system in diabetic nephropathy 被引量:35
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作者 Tanuj Chawla Deepika Sharma Archana Singh 《World Journal of Diabetes》 SCIE CAS 2010年第5期141-145,共5页
Diabetic nephropathy has been the cause of lot of morbidity and mortality in the diabetic population. The renin angiotensin system (RAS) is considered to be involved in most of the pathological processes that result i... Diabetic nephropathy has been the cause of lot of morbidity and mortality in the diabetic population. The renin angiotensin system (RAS) is considered to be involved in most of the pathological processes that result in diabetic nephropathy. This system has various subsystems which contribute to the disease pathology. One of these involves angiotensin II (Ang II) which shows increased activity during diabetic nephropathy. This causes hypertrophy of various renal cells and has a pressor effect on arteriolar smooth muscle resulting in increased vascular pressure. Ang II also induces inflammation, apoptosis, cell growth, migration and differentiation. Monocyte chemoattractant protein-1 production responsible for renal fibrosis is also regulated by RAS. Polymorphism of angiotensin converting enzyme (ACE) and Angiotensinogen has been shown to have effects on RAS. Available treatment modalities have proven effective in controlling the progression of nephropathy. Various drugs (based on antagonism of RAS) are currently in the market and others are still under trial. Amongst the approved drugs, ACE inhibitors and angiotensin receptor blockers (ARBs) are widely used in clinical practice. ARBs are shown to be superior to ACE inhibitors in terms of reducing proteinuria but the combined role of ARBs with ACE inhibitors in diabetic nephropathy is under debate. 展开更多
关键词 Diabetic nephropathy Angiotensin II Monocyte chemoattractant protein-1 Renin angiotensin system
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Spinal CCL2 Promotes Central Sensitization, Long-Term Potentiation, and Inflammatory Pain via CCR2: Further Insights into Molecular, Synaptic, and Cellular Mechanisms 被引量:20
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作者 Rou-Gang Xie Yong-Jing Gao +5 位作者 Chul-Kyu Park Ning Lu Ceng Luo Wen-Ting Wang Sheng-Xi Wu Ru-Rong Ji 《Neuroscience Bulletin》 SCIE CAS CSCD 2018年第1期13-21,共9页
Mounting evidence supports an important role of chemokines, produced by spinal cord astrocytes, in promoting central sensitization and chronic pain. In particular, CCL2 (C-C motif chemokine ligand 2) has been shown ... Mounting evidence supports an important role of chemokines, produced by spinal cord astrocytes, in promoting central sensitization and chronic pain. In particular, CCL2 (C-C motif chemokine ligand 2) has been shown to enhance N-methyl-D-aspartate (NMDA)-induced currents in spinal outer lamina II (Iio) neurons. However, the exact molecular, synaptic, and cellular mechanisms by which CCL2 modulates central sensitization are still unclear. We found that spinal injection of the CCR2 antagonist RS504393 attenuated CCL2- and inflammation-induced hyperalgesia. Single-cell RT-PCR revealed CCR2 expres- sion in excitatory vesicular glutamate transporter subtype 2-positive (VGLUT2+) neurons. CCL2 increased NMDA- induced currents in CCR2+/VGLUT2+ neurons in lamina IIo; it also enhanced the synaptic NMDA currents evoked by dorsal root stimulation; and furthermore, it increased the total and synaptic NMDA currents in somatostatin- expressing excitatory neurons. Finally, intrathecal RS504393 reversed the long-term potentiation evoked in the spinal cord by C-fiber stimulation. Our findings suggest that CCL2 directly modulates synaptic plasticity in CCR2- expressing excitatory neurons in spinal lamina Iio, and this underlies the generation of central sensitization in patho- logical pain. 展开更多
关键词 CHEMOKINES C-C motif chemokine ligand 2 (CCL2) Monocyte chemoattractant protein 1 (MCP-1) Neuron-glial interaction
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Role of monocytes and macrophages in experimental and human acute liver failure 被引量:13
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作者 Lucia A Possamai Charalambos Gustav Antoniades +4 位作者 Quentin M Anstee Alberto Quaglia Diego Vergani Mark Thursz Julia Wendon 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第15期1811-1819,共9页
Acute liver failure (ALF) is a devastating clinical syndrome characterised by progressive encephalopathy, coagulopathy, and circulatory dysfunction, which commonly leads to multiorgan failure and death. Central to the... Acute liver failure (ALF) is a devastating clinical syndrome characterised by progressive encephalopathy, coagulopathy, and circulatory dysfunction, which commonly leads to multiorgan failure and death. Central to the pathogenesis of ALF is activation of the immune system with mobilisation of cellular effectors and massive production of cytokines. As key components of the innate immune system, monocytes and macrophages are postulated to play a central role in the initiation, progression and resolution of ALF. ALF in humans follows a rapidly progressive clinical course that poses inherent difficulties in delineating the role of these pivotal immune cells. Therefore, a number of experimental models have been used to study the pathogenesis of ALF. Here we consider the evidence from experimental and human studies of ALF on the role of monocytes and macrophages in acute hepatic injury and the ensuing extrahepatic manifestations, including functional monocyte deactivation and multiple organ failure. 展开更多
关键词 MONOCYTE Macrophage Acute liver failure Inflammation Monocyte chemoattractant protein-1/ chemokine (C-C motif) receptor-2 CYTOKINE
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丹参多酚酸盐治疗老年糖尿病肾病的临床研究 被引量:15
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作者 赵琪 冯春玲 +3 位作者 孙玉珍 刘健 张雪 李晓萌 《现代药物与临床》 CAS 2015年第4期441-444,共4页
目的通过研究丹参多酚酸盐对老年糖尿病肾病的疗效及对尿N-乙酰β-D-葡萄糖苷酶(NAG)、转化生长因子-β1(TGF-β1)、单核细胞趋化蛋白-1(MCP-1)的影响,探讨丹参多酚酸盐治疗糖尿病肾病的机制及糖尿病肾病发病过程中的关键因子。方法 90... 目的通过研究丹参多酚酸盐对老年糖尿病肾病的疗效及对尿N-乙酰β-D-葡萄糖苷酶(NAG)、转化生长因子-β1(TGF-β1)、单核细胞趋化蛋白-1(MCP-1)的影响,探讨丹参多酚酸盐治疗糖尿病肾病的机制及糖尿病肾病发病过程中的关键因子。方法 90例糖尿病肾病患者随机分为2组,每组45例,对照组给予常规治疗,治疗组在对照组的基础上增加丹参多酚酸盐治疗,将200 mg注射用丹参多酚酸盐加入200 m L生理盐水中,静脉滴注,1次/d,4周为1个疗程。比较两组患者的疗效及NAG、尿微量白蛋白(m ALB)、β2-微球蛋白(β2-MG)、TGF-β1、MCP-1和生化指标的变化。结果治疗后对照组和治疗组的有效率分别为82.22%、91.11%,两组比较差异具有统计学意义(P<0.05)。治疗后,两组的尿白蛋白排泄率(UAER)、肌酐(Cr)、血尿素氮(BUN)均较治疗前明显好转,且差异具有统计学意义(P<0.01);与对照组治疗后比较,治疗组的UAER、纤维蛋白原(FIB)明显改善,且差异具有统计学意义(P<0.01)。治疗后,两组的NAG、m ALB、β2-MG、TGF-β1和MCP-1均明显好转,与治疗前相比差异具有统计学意义(P<0.01);与对照组治疗后比较,治疗组的NAG、β2-MG、TGF-β1和MCP-1显著改善,且差异具有统计学意义(P<0.01),两组的m ALB差异不具有统计学意义。结论丹参多酚酸盐对糖尿病肾病的治疗作用主要通过调节TGF-β1和MCP-1水平、延缓近端肾小管损伤实现。 展开更多
关键词 丹参多酚酸盐 N-乙酰β-D-葡萄糖苷酶(NAG) β2-微球蛋白(β2-MG) 转化生长因子-β1(TGF-β1) 单核细胞趋化蛋白-1(MCP-1) N-acetylβ-D-glycosidase enzymes (NAG) Β2-MICROGLOBULIN (β2-MG) transforming growth factor-β1(TGF-β1) monocyte chemoattractANT protein-1 (MCP 1)
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Advanced oxidation protein products induce monocyte chemoattractant protein-1 expression via p38 mitogen-activated protein kinase activation in rat vascular smooth muscle cells 被引量:10
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作者 PENG Kan-fu WU Xiong-fei ZHAO Hong-wen SUN Yan 《Chinese Medical Journal》 SCIE CAS CSCD 2006年第13期1088-1093,共6页
Background Advanced oxidation protein products (AOPPs) are new uremic toxins reported by Witko-Sarsat in 1996, which are associated with the pathogenesis of atherosclerosis. However, the mechanisms by which AOPPs en... Background Advanced oxidation protein products (AOPPs) are new uremic toxins reported by Witko-Sarsat in 1996, which are associated with the pathogenesis of atherosclerosis. However, the mechanisms by which AOPPs enhance atherosclerosis have not been fully understood. Monocyte chemoattractant protein-1 (MCP-1) is a chemokine which stimulates migration of monocytes and plays a critical role in the development of atherosclerosis. In this study, we investigated the effect of AOPPs on MCP-1 expression in cultured vascular smooth muscle cells (VSMCs). 展开更多
关键词 ATHEROSCLEROSIS advanced oxidation protein products monocyte chemoattractant protein-1 mitogen-activated protein kinase myocytes smooth muscle
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Neutrophil chemoattractant receptors in health and disease: double-edged swords 被引量:14
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作者 Mieke Metzemaekers Mieke Gouwy Paul Proost 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2020年第5期433-450,共18页
Neutrophils are frontline cells of the innate immune system.These effector leukocytes are equipped with intriguing antimicrobial machinery and consequently display high cytotoxic potential.Accurate neutrophil recruitm... Neutrophils are frontline cells of the innate immune system.These effector leukocytes are equipped with intriguing antimicrobial machinery and consequently display high cytotoxic potential.Accurate neutrophil recruitment is essential to combat microbes and to restore homeostasis,for inflammation modulation and resolution,wound healing and tissue repair.After fulfilling the appropriate effector functions,however,dampening neutrophil activation and infiltration is crucial to prevent damage to the host.In humans,chemoattractant molecules can be categorized into four biochemical families,i.e.,chemotactic lipids,formyl peptides,complement anaphylatoxins and chemokines.They are critically involved in the tight regulation of neutrophil bone marrow storage and egress and in spatial and temporal neutrophil trafficking between organs.Chemoattractants function by activating dedicated heptahelical G protein-coupled receptors(GPCRs).In addition,emerging evidence suggests an important role for atypical chemoattractant receptors(ACKRs)that do not couple to G proteins in fine-tuning neutrophil migratory and functional responses.The expression levels of chemoattractant receptors are dependent on the level of neutrophil maturation and state of activation,with a pivotal modulatory role for the(inflammatory)environment.Here,we provide an overview of chemoattractant receptors expressed by neutrophils in health and disease.Depending on the(patho)physiological context,specific chemoattractant receptors may be up-or downregulated on distinct neutrophil subsets with beneficial or detrimental consequences,thus opening new windows for the identification of disease biomarkers and potential drug targets. 展开更多
关键词 NEUTROPHILS chemoattractANT G protein-coupled receptors CHEMOKINE leukocyte migration
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Relationship between vitamin D and IL-23,IL-17 and macrophage chemoattractant protein-1 as markers of fibrosis in hepatitis C virus Egyptians 被引量:12
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作者 Noha M El Husseiny Hala M Fahmy +1 位作者 Waleed A Mohamed Hisham H Amin 《World Journal of Hepatology》 CAS 2012年第8期242-247,共6页
AIM: To assess vitamin D in hepatitis C patients and its relationship to interleukin (IL)-23, IL-17, and macrophage chemoattractant protein-1 (MCP-1). METHODS: The study was conducted on 50 Egyptian hepatitis C virus ... AIM: To assess vitamin D in hepatitis C patients and its relationship to interleukin (IL)-23, IL-17, and macrophage chemoattractant protein-1 (MCP-1). METHODS: The study was conducted on 50 Egyptian hepatitis C virus (HCV) genotype number IV-infected patients and 25 age- and gender-matched healthy subjects. Venous blood samples were obtained. Samples were allowed to clot and sera were separated by centrifugation and stored at -20?°C. A 25 hydroxy vitamin D assay was carried out using solid phase RIA. A 1,25 dihydroxy vitamin D assay was carried out using a commercial kit purchased from Incstar Corporation. IL-17 and -23 and MCP-1 were assayed by an enzyme immunoassay. Quantitative and qualitative polymerase chain reaction for HCV virus were done by TaqMan technology. Only HCV genotype IV-infected subjects were included in the study. The mean ± SD were determined, a t-test for comparison of means of different parameters was used. Correlation analysis was done using Pearson’s correlation. Differences among different groups were determined using the Kruskal-Wallis test. RESULTS: The mean vitamin D level in HCV patients (group?I) was 15 ± 5.2 ng/mL while in control (group II) was 39.7 ± 10.8. For active vitamin D in group?I?as 16.6 ± 4.8 ng/mL while in group II was 41.9 ± 7.9. IL-23 was 154 ± 97.8 in group?I?and 6.7 ± 2.17 in group II. IL-17 was 70.7 ± 72.5 in cases and 1.2 ± 0.4 in control. MCP-1 was 1582 ± 794.4 in group?I?and 216.1 ± 5.38 in group II. Vitamin D deficiency affected 72% of HCV-infected patients and 0% of the control group. Vitamin D insufficiency existed in 28% of HCV-infected patients and 12% of the control group. One hundred percent of the cirrhotic patients and 40% of non cirrhotic HCV-infected patients had vitamin D deficiency. IL-23, IL-17, and MCP-1 were markedly increased in HCV-infected patients in comparison to controls.A significant negative correlation between vitamin D and IL-17 and -23 and MCP-1 was detected. HCV-infected males and females showed no differences with respec 展开更多
关键词 Vitamin D Macrophage chemoattractant protin-1 Liver cirrhosis INTERLEUKIN-23 INTERLEUKIN-17 Liver cirrhosis
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Metformin inhibits nuclear factor-κB activation and inflammatory cytokines expression induced by high glucose via adenosine monophosphate-activated protein kinase activation in rat glomerular mesangial cells in vitro 被引量:9
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作者 Gu Junfei Ye Shandong Wang Shan Sun Wenjia Hu Yuanyuan 《Chinese Medical Journal》 SCIE CAS CSCD 2014年第9期1755-1760,共6页
Background The renoprotective mechanisms of adenosine monophosphate (AMP)-activated protein kinase (AMPK) agonist-metformin have not been stated clearly.We hypothesized that metformin may ameliorate inflammation v... Background The renoprotective mechanisms of adenosine monophosphate (AMP)-activated protein kinase (AMPK) agonist-metformin have not been stated clearly.We hypothesized that metformin may ameliorate inflammation via AMPK interaction with critical inflammatory cytokines The aim of this study was to observe the effects of metformin on expression of nuclear factor-κB (NF-κB),monocyte chemoattractant protein-1 (MCP-1),intercellular adhesion molecule-1 (ICAM-1) and transforming growth factor-beta 1 (TGF-β1) induced by high glucose (HG) in cultured rat glomerular mesangial cells (MCs).Methods MCs were cultured in the medium with normal concentration glucose (group NG,5.6 mmol/L),high concentration glucose (group HG,25 mmol/L) and different concentrations of metformin (group M1,M2,M3).After 48-hour exposure,the supernatants and MCs were collected.The expression of NF-κB,MCP-1,ICAM-1,and TGF-β1 mRNA was analyzed by real time polymerase chain reaction.Westem blotting was used to detect the expression of AMPK,phospho-Thr-172 AMPK (p-AMPK),NF-κB p65,MCP-1,ICAM-1,and TGF-β1 protein.Results After stimulated by HG,the expression of NF-κB,MCP-1,ICAM-1,TGF-β1 mRNA and protein of MCs in group HG increased significantly compared with group NG (P <0.05).Both genes and protein expression of NF-κB,MCP-1,ICAM-1,TGF-β1 of MCs induced by high glucose were markedly reduced after metformin treatment in a dose-dependent manner (P <0.05).The expression of p-AMPK increased with the rising of metformin concentration,presenting the opposite trend,while the level of total-AMPK protein was unchanged with exposure to HG or metformin.Conlusion Metformin can suppress the expression of NF-κB,MCP-1,ICAM-1 and TGF-β1 of glomerular MCs induced by high glucose via AMPK activation,which may partlv contribute to its reno-protection. 展开更多
关键词 METFORMIN adenosine monophosphate-activated protein kinase nuclear factor-κB monocyte chemoattractant protein-1 intercellular adhesion molecule-1 transforming growth factor-beta 1 glomerular mesangial cell
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Calcitonin gene-related peptide induces proliferation and monocyte chemoattractant protein-1 expression via extracellular signal-regulated kinase activation in rat osteoblasts 被引量:9
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作者 HAN Na ZHANG Dian-ying WANG Tian-bing ZHANG Pei-xun JIANG Bao-guo 《Chinese Medical Journal》 SCIE CAS CSCD 2010年第13期1748-1753,共6页
Background Calcitonin gene-related peptide (CGRP), a sensory neuropeptide, affects osteoblast proliferation and bone formation. However, the mechanisms are not fully understood. Monocyte chemoattractant protein-1 (... Background Calcitonin gene-related peptide (CGRP), a sensory neuropeptide, affects osteoblast proliferation and bone formation. However, the mechanisms are not fully understood. Monocyte chemoattractant protein-1 (MCP-1) is a chemokine that stimulates the migration of monocytes and plays important roles in regulating bone remolding during fracture repair. In this study, we investigated the effects of CGRP on proliferation and MCP-1 expression in cultured rat osteoblasts. Methods Primary rat osteoblasts were isolated from fetal rats calvariae. Cells were exposed to gradient concentrations (10^-9 to 10^-7 mol/L) of CGRP. Protein and mRNA levels of MCP-1 were quantified by Western blotting and semiquantitative reverse transcdption-polymerase chain reaction, respectively. The protein level of MCP-1 was investigated and compared in cell culture media by enzyme linked immunosorbent assay (ELISA). Phospho-extracellular signal-regulated kinase (ERK) expression was detected by Western blotting. Cell proliferative activity was measured by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and BrdU assay. The effects of MAPK/ERK kinase (MEK)-inhibitor U0126 on CGRP-induced MCP-1 expression in primary rat osteoblasts were examined. Results CGRP effectively enhanced primary rat osteoblast proliferation and led to significant increases in the expression of MCP-1 mRNA and protein in time- and dose-dependent manners. CGRP activated the ERK pathway. Pretreatment of cultured rat osteoblasts with MEK inhibitor U0126 resulted in dose-dependent inhibitions of CGRP-induced MCP-1 mRNA and protein levels. Thus, CGRP promoted cell proliferation and stimulated MCP-1 expression in cultured rat osteoblasts. Conclusion These studies document novel links between CGRP and MCP-1 and illuminate the effects of CGRP in regulating bone remodeling. 展开更多
关键词 calcitonin gene-related peptide monocyte chemoattractant protein-I rat primary osteoblasts extracellular signal-regulated kinase
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Effect of Danzhijiangtang capsule on monocyte chemoattractant protein-1 mRNA expression in newly diagnosed diabetes subclinical vascular lesions 被引量:9
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作者 Zhao-Hui Fang Yan Liu +6 位作者 Tao-Tao Bao Ying-Qun Ni Jian Liu Guo-Bin Shi Ji-Ping Wu Jun-Ping Yang Hong Zhang 《World Journal of Gastroenterology》 SCIE CAS 2013年第19期2963-2968,共6页
AIM:To investigate the effect of Danzhijiangtang capsule(DJC) on monocyte chemoattractant protein-1(MCP-1) mRNA expression in newly diagnosed type 2 diabetes mellitus(T2DM) subclinical vascular lesions.METHODS:Sixty-t... AIM:To investigate the effect of Danzhijiangtang capsule(DJC) on monocyte chemoattractant protein-1(MCP-1) mRNA expression in newly diagnosed type 2 diabetes mellitus(T2DM) subclinical vascular lesions.METHODS:Sixty-two patients with newly diagnosed T2DM subclinical vascular lesions were randomly divided into a control group and treatment group of 31 cases each.Oral antidiabetic therapy with routine western medicine was conducted in both groups,and the treatment group was additionally treated with DJCs.The treatment course for both groups was 12 wk.Before and after treatment,the total efficiency and traditional Chinese medicine(TCM) syndrome score were calculated.The fasting plasma glucose(FPG),2-h plasma glucose(2hPG),fasting insulin(FINS),insulin resistance index(IRI),hemoglobin(Hb)A1c,blood lipids,and hemorheology indices were determined.In addition,the levels of vascular endothelial growth factors including thrombomodulin(TM),von Willebrand factor(vWF),P-selectin and MCP-1 mRNA were determined.RESULTS:After 12 wk of treatment,the TCM syndrome score was significantly decreased compared to before treatment in both groups.After treatment,FPG,2hPG,HbA1c,FINS,IRI,total cholesterol,triglycerides,low-density lipoprotein,high-density lipoprotein,whole blood low shear specific viscosity,plasma specific viscosity,TM,vWF,P-selectin and MCP-1 mRNA were significantly improved compared to before treatment in both groups.After treatment,the total efficiency and TCM syndrome score in the treatment group were better than in the control group.FINS,IRI,whole blood high shear specific viscosity,plasma specific viscosity,TM,vWF,P-selectin and MCP-1 mRNA level in the treatment group were significantly reduced after treatment compared with control group.CONCLUSION:DJCs are efficacious in supplementing qi,nourishing yin and invigorating blood circulation,and upregulate MCP-1 mRNA expression in patients with T2DM subclinical vascular lesions. 展开更多
关键词 Danzhijiangtang CAPSULE Type 2 DIABETES MELLITUS SUBCLINICAL vascular lesions MONOCYTE chemoattractANT protein-1
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Salidroside Inhibits Lipopolysaccharide-ethanol-induced Activation of Proinflammatory Macrophages via Notch Signaling Pathway 被引量:11
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作者 Jian-sha LI Lu-yao FAN +1 位作者 Meng-dan YUAN Ming-you XING 《Current Medical Science》 SCIE CAS 2019年第4期526-533,共8页
Activation of macrophages is a key event for the pathogenesis of various inflammatory diseases.Notch signaling pathway recently has been found to be a critical pathway in the activation of proinflammatory macrophages.... Activation of macrophages is a key event for the pathogenesis of various inflammatory diseases.Notch signaling pathway recently has been found to be a critical pathway in the activation of proinflammatory macrophages.Salidroside (Sal),one of main bioactive components in Rhodiola crenulata (Hook.F.et Thoms) H.ohba,reportedly possesses anti-inflammatory activity and ameliorates inflammation in alcohol-induced hepatic injury.However,whether Sal regulates the activation of proinflammatory macrophages through Notch signaling pathway remains unknown.The present study investigated the effects of Sal on macrophage activation and its possible mechanisms by using both alcohol and lipopolysaccharide (LPS) to mimic the microenvironment of alcoholic liver.Detection of THP-1-derived macrophages exhibited that Sal could significantly decrease the expression of tumor necrosis factor-α(TNF-α),interleukinbeta (IL-1β)and IL-6 in the macrophages at both mRNA and protein levels.Furthermore,Sal significantly suppressed NF-kB activation via Notch-Hes signaling pathway in a dose-dependent manner.Moreover,in the microenvironment of alcoholic liver,the expression of Notch-dependent pyruvate dehydrogenase phosphatase 1 (PDP1) was elevated,and that of Ml gene expression [inducible NO synthase (NOS2)] was up-regulated.These changes could all be effectively ameliorated by Sal.The aforementioned findings demonstrated that Sal could inhibit LPS-ethanol-induced activation of proinflammatory macrophages via Notch signaling pathway. 展开更多
关键词 THP-1 MACROPHAGES SALIDROSIDE Notch tumor necrosis factor-α MONOCYTE chemoattractANT protei-1
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Chemokine signaling involving chemokine (C-C motif) ligand 2 plays a role in descending pain facilitation 被引量:8
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作者 Ronald Dubner 《Neuroscience Bulletin》 SCIE CAS CSCD 2012年第2期193-207,共15页
Objective Despite accumulating evidence on a role of immune cells and their associated chemicals in mecha- nisms of pain, few studies have addressed the potential role of chemokines in the descending facilitation of p... Objective Despite accumulating evidence on a role of immune cells and their associated chemicals in mecha- nisms of pain, few studies have addressed the potential role of chemokines in the descending facilitation of persistent pain. The present study was undertaken to test the hypothesis that the chemokine (C-C motif) ligand 2 (CCL2) (commonly known as monocyte chemoattractant protein-1) signaling in the rostral ventromedial medulla (RVM), a pivotal structure in brainstem pain modulatory circuitry, is involved in descending pain facilitation in rats. Methods An L5 spinal nerve ligation (SNL) was produced in rats under pentobarbital anesthesia. Western blot and immunohistochemistry were used to detect the expression levels of CCL2 and CCL2 receptor (CCR2), and examine their distributions compared with the neuronal marker NeuN as well as glial markers glial fibrillary acidic protein (GFAP, astroglial) and CD 11 b (microglial), respectively. Results SNL induced an increase in CCL2 expression in the RVM, and this returned to the control level at 4 weeks after injury. The induced CCL2 colocalized with NeuN, but not with GFAP and CD1 lb. CCR2 was also upregu- lated by SNL in the RVM, and this increase lasted for at least 4 weeks. CCR2 was colocalized with CD1 lb but not GFAP. Few RVM neurons also exhibited CCR2 staining. Neutralizing CCL2 with an anti-CCL2 antibody (0.2-20 ng) or injecting RS-102895 (0.1-10 pmol), a CCR2b chemokine receptor antagonist, into the RVM on day 1 after SNL, significantly at- tenuated the established thermal and mechanical hypersensitivity. In addition, injection of recombinant rat CCL2 (0.03-3 pmol) into the RVM induced dose-dependent hyperalgesia, which was prevented by pretreatment with RS-102895 (10 pmol). Interleukin-β (IL-1]3), a potent inducer of neuronal CCL2, was also selectively upregulated in RVM reactive as- trocytes. Injection of IL-1 ]3 (120 fmol) into the RVM induced behavioral hyperalgesia, which was blocked by RS-102895 展开更多
关键词 monocyte chemoattractant protein-l chemokine (C-C motif) receptor 2 rostral ventromedial medulla neu-ron-glial interaction neuropathic pain rat
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Effects of Bifidobacterium infantis on cytokine-induced neutrophil chemoattractant and insulin-like growth factor-1 in the ileum of rats with endotoxin injury 被引量:7
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作者 Wei Wang Mei Sun +2 位作者 Yu-Ling Zheng Liu-Yu Sun Shu-Qiang Qu 《World Journal of Gastroenterology》 SCIE CAS 2019年第23期2924-2934,共11页
BACKGROUND The digestive tract is the maximal immunizing tissue in the body, and mucosal integrity and functional status of the gut is very important to maintain a healthy organism. Severe infection is one of the most... BACKGROUND The digestive tract is the maximal immunizing tissue in the body, and mucosal integrity and functional status of the gut is very important to maintain a healthy organism. Severe infection is one of the most common causes of gastrointestinal dysfunction, and the pathogenesis is closely related to endotoxemia and intestinal barrier injury. Bifidobacterium is one of the main probiotics in the human body that is involved in digestion, absorption, metabolism, nutrition, and immunity.Bifidobacterium plays an important role in maintaining the intestinal mucosal barrier integrity. This study investigated the protective mechanism of Bifidobacterium during ileal injury in rats.AIM To investigate the effects of Bifidobacterium on cytokine-induced neutrophil chemoattractant(CINC) and insulin-like growth factor 1(IGF-1) in the ileum of rats with endotoxin injury.METHODS Preweaning rats were randomly divided into three groups: Control(group C),model(group E) and treatment(group T). Group E was intraperitoneally injected with lipopolysaccharide(LPS) to create an animal model of intestinal injury.Group T was intragastrically administered Bifidobacterium suspension 7 d before LPS. Group C was intraperitoneally injected with normal saline. The rats were killed at 2, 6 or 12 h after LPS or physiological saline injection to collect ilealtissue samples. The expression of ileal CINC mRNA was evaluated by reverse transcription-polymerase chain reaction(RT-PCR), and expression of ileal IGF-1 protein and mRNA was detected by immunohistochemistry and RT-PCR,respectively.RESULTS The ileum of rats in Group C did not express CINC mRNA, ileums from Group E expressed high levels, which was then significantly decreased in Group T(F =23.947, P < 0.05). There was no significant difference in CINC mRNA expression at different times(F = 0.665, P > 0.05). There was a high level of IGF-1 brown granules in ileal crypts and epithelial cells in Group C, sparse staining in Group E, and dark, dense brown staining in Group T. There was a signifi 展开更多
关键词 BIFIDOBACTERIUM ILEUM Cytokine-induced neutrophil chemoattractANT INSULIN-LIKE growth factor-1 RATS
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Passive cigarette smoking induces inflammatory injury in human arterial walls 被引量:7
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作者 ZOU Ni HONG Jiang DAI Qiu-yan 《Chinese Medical Journal》 SCIE CAS CSCD 2009年第4期444-448,共5页
Background Epidemiological studies have shown that both active and passive cigarette smoking increase the risk of atherosclerosis. But very little is known about the biological processes induced by passive cigarette s... Background Epidemiological studies have shown that both active and passive cigarette smoking increase the risk of atherosclerosis. But very little is known about the biological processes induced by passive cigarette smoking that contribute to atherosclerosis. We observe the expression of a few of biological and inflammatory markers in human arterial walls in vitro which were treated with the second-hand smoke solution (sidestream whole, SSW), and discuss the possible mechanism of inflammatory injury induced by second-hand smoke. Methods The biological markers (platelet endothelial cell adhesion molecule-I, PECAM-1; a-smooth muscle actin, a-SMA; collagen IV, Col IV) and inflammatory markers (vascular cell adhesion molecule-1, VCAM-1; monocyte chemoattractant protein-1, MCP-1 ; interleukin-8, IL-8) of human aortat wall were tested by immunofluorescence staining. The levels of MCP-1 and IL-8 mRNA expression were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results No distinct difference was observed between SSW and the control group on the expression of biological markers as assessed by the light microscope. But the inflammatory markers VCAM-1, MCP-1 and IL-8 on the subendothelial layer and smooth muscle cell layers, which are near the endothelium of arterial wall, were strongly stained in the SSW group compared with the control group. Their fluorescence intensities in the 1:40 SSW group (VCAM-1: 0.35±0.04, MCP-1: 0.34±0.05, IL-8: 0.37±0.05) and the 1:20 SSW group (VCAM-I: 0.40±0.04, MCP-1: 0.52±0.09, IL-8: 0.51±0.07) were significantly stronger than the control group (VCAM-1: 0.12±0.04, MCP-1: 0.06±0.02, IL-8: 0.24±0.03) by semi-quantitative analysis of immunofluorescence (P 〈0.001 vs control). MCP-1 mRNA expression in the 1:40 SSW (0.15±0.04) and the 1:20 SSW (0.19±0.06) group was significantly higher than in the control group (0.09±0.03) (P 〈0.05, P 〈0.01 vs control); IL-8 mRNA expression in the 1� 展开更多
关键词 passive cigarette smoking ATHEROSCLEROSIS inflammatory injury vascular cell adhesion molecule-I monocyte chemoattractant protein-l INTERLEUKIN-8
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