The aim of this study is to compare the effects of single and combined oral administration of fresh aqueous extracts of Acalypha wilkesiana supplementation at 300 mg/ml and exercise training on some immuno indicator p...The aim of this study is to compare the effects of single and combined oral administration of fresh aqueous extracts of Acalypha wilkesiana supplementation at 300 mg/ml and exercise training on some immuno indicator parameters in Rats. The study was carried out in the College of Health Sciences, Department of Hematology, Igbinedion University, Okada between the Month of June and July, 2012. Following 30 days post-oral administration of extracts on (Group 2 and Group 3), exercise training on (Group 2 and Group 4) and No treated on Group 1, hematological parameters were determined using the sysmex®?Automated Hematological Analyzer, while CD4 and CD8 cells were estimated using Partec cyflow counter and serum IgG is determined using the ELISA Method. CD4 cells, CD8, and Total WBC count show a statistically significant increase while Hb concentration and IgG level show a statistically significant decrease in Group 2 and Group 4 (P < 0.05). Total WBC count shows a statistically significant increase against the control Group, while CD4 cell, CD8 cell count, Hb-concentration and IgG level show a statistically insignificant increase (P > 0.05), Table 2. Acalypha wilkesiana at a concentration of 300 mg/g/day seems to be immuno-protective in Rats. Single or combine effects of prolonged exercise and A. wilkesiana produce significant change in some immuno indicators parameters. However, the molecular mechanism behind their combined effect would require further investigation.展开更多
背景与目的CIK细胞是过继免疫治疗的重要手段之一,简化体外培养过程从而提高其增殖率和杀瘤活性仍是目前研究的一个热点课题。本研究观察重组人纤维连接蛋白(recombinant human fibronectin,RN)诱导CIK细胞的生物学特性,建立一种高效、...背景与目的CIK细胞是过继免疫治疗的重要手段之一,简化体外培养过程从而提高其增殖率和杀瘤活性仍是目前研究的一个热点课题。本研究观察重组人纤维连接蛋白(recombinant human fibronectin,RN)诱导CIK细胞的生物学特性,建立一种高效、简便的体外CIK细胞扩增方法。方法抽取10名健康人外周静脉血各50mL,用淋巴细胞分离液分离单个核细胞,分别采用RN诱导法和传统方法培养CIK细胞,记录细胞增殖数;用流式细胞术测定免疫细胞表型和分泌IFN-γ、IL-4、穿孔素和颗粒酶B细胞的百分比;用MTT法测定CIK细胞对4种人肺癌细胞株的体外杀伤率。结果RN诱导的CIK细胞扩增倍数为传统方法的2.0倍-3.5倍,具有统计学差异(P<0.05);RN诱导组和传统方法组CD3+CD16+CD56+细胞绝对数分别增加了3778倍和2069倍;RN诱导组细胞中CD3+CD8+细胞比例明显高于传统方法组(P<0.05);但CD3+CD4+细胞比例无统计学差异(P>0.05);对4种肺癌细胞株的体外杀伤活性无统计学差异(P>0.05)。RN诱导的CIK较诱导前:分泌IFN-γ的细胞比例明显增加;分泌IL-4的细胞比例略有降低;释放穿孔素、颗粒酶B的阳性细胞比例较诱导前增加。结论RN诱导法是一种高效、简便的体外扩增CIK方法,可以替代传统方法。展开更多
Liver sinusoidal endothelial cells （LSECs） line the liver sinusoids and separate passenger leukocytes in the sinusoidal lumen from hepatocytes. LSECs further act as a platform for adhesion of various liver-resident ...Liver sinusoidal endothelial cells （LSECs） line the liver sinusoids and separate passenger leukocytes in the sinusoidal lumen from hepatocytes. LSECs further act as a platform for adhesion of various liver-resident immune cell populations such as Kupffer cells, innate lymphoid cells or liver dendritic cells. In addition to having an extraordinary scavenger function, LSECs possess potent immune functions, serving as sentinel cells to detect microbial infection through pattern recognition receptor activation and as antigen （cross）-presenting cells. LSECs cross-prime naive CD8 T cells, causing their rapid differentiation into memory T cells that relocate to secondary lymphoid tissues and provide protection when they re-encounter the antigen during microbial infection. Cross-presentation of viral antigens by LSECs derived from infected hepatocytes triggers local activation of effector CD8 T cells and thereby assures hepatic immune surveillance. The immune function of LSECs complements conventional immune-activating mechanisms to accommodate optimal immune surveillance against infectious microorganisms while preserving the integrity of the liver as a metabolic organ.展开更多
Hepatitis B virus(HBV)infection is a major cause of chronic liver diseases that may progress to liver cirrhosis and hepatocellular carcinoma.Host immune responses are important factors that determine whether HBV infec...Hepatitis B virus(HBV)infection is a major cause of chronic liver diseases that may progress to liver cirrhosis and hepatocellular carcinoma.Host immune responses are important factors that determine whether HBV infection is cleared or persists.After infection,viral replication occurs inside hepatocytes,and the secretion of infectious virions can take place at high rates for decades.Consequently,HBV DNA and viral proteins,like HBV early antigen(HBeAg)and HBV surface antigen(HBsAg),can be easily detected in serum.Chronic infection with HBV is the result of an ineffective antiviral immune response towards the virus.In this review,we discuss the role of immune cells in chronic HBV infection.展开更多
文摘The aim of this study is to compare the effects of single and combined oral administration of fresh aqueous extracts of Acalypha wilkesiana supplementation at 300 mg/ml and exercise training on some immuno indicator parameters in Rats. The study was carried out in the College of Health Sciences, Department of Hematology, Igbinedion University, Okada between the Month of June and July, 2012. Following 30 days post-oral administration of extracts on (Group 2 and Group 3), exercise training on (Group 2 and Group 4) and No treated on Group 1, hematological parameters were determined using the sysmex®?Automated Hematological Analyzer, while CD4 and CD8 cells were estimated using Partec cyflow counter and serum IgG is determined using the ELISA Method. CD4 cells, CD8, and Total WBC count show a statistically significant increase while Hb concentration and IgG level show a statistically significant decrease in Group 2 and Group 4 (P < 0.05). Total WBC count shows a statistically significant increase against the control Group, while CD4 cell, CD8 cell count, Hb-concentration and IgG level show a statistically insignificant increase (P > 0.05), Table 2. Acalypha wilkesiana at a concentration of 300 mg/g/day seems to be immuno-protective in Rats. Single or combine effects of prolonged exercise and A. wilkesiana produce significant change in some immuno indicators parameters. However, the molecular mechanism behind their combined effect would require further investigation.
文摘背景与目的CIK细胞是过继免疫治疗的重要手段之一,简化体外培养过程从而提高其增殖率和杀瘤活性仍是目前研究的一个热点课题。本研究观察重组人纤维连接蛋白(recombinant human fibronectin,RN)诱导CIK细胞的生物学特性,建立一种高效、简便的体外CIK细胞扩增方法。方法抽取10名健康人外周静脉血各50mL,用淋巴细胞分离液分离单个核细胞,分别采用RN诱导法和传统方法培养CIK细胞,记录细胞增殖数;用流式细胞术测定免疫细胞表型和分泌IFN-γ、IL-4、穿孔素和颗粒酶B细胞的百分比;用MTT法测定CIK细胞对4种人肺癌细胞株的体外杀伤率。结果RN诱导的CIK细胞扩增倍数为传统方法的2.0倍-3.5倍,具有统计学差异(P<0.05);RN诱导组和传统方法组CD3+CD16+CD56+细胞绝对数分别增加了3778倍和2069倍;RN诱导组细胞中CD3+CD8+细胞比例明显高于传统方法组(P<0.05);但CD3+CD4+细胞比例无统计学差异(P>0.05);对4种肺癌细胞株的体外杀伤活性无统计学差异(P>0.05)。RN诱导的CIK较诱导前:分泌IFN-γ的细胞比例明显增加;分泌IL-4的细胞比例略有降低;释放穿孔素、颗粒酶B的阳性细胞比例较诱导前增加。结论RN诱导法是一种高效、简便的体外扩增CIK方法,可以替代传统方法。
文摘Liver sinusoidal endothelial cells （LSECs） line the liver sinusoids and separate passenger leukocytes in the sinusoidal lumen from hepatocytes. LSECs further act as a platform for adhesion of various liver-resident immune cell populations such as Kupffer cells, innate lymphoid cells or liver dendritic cells. In addition to having an extraordinary scavenger function, LSECs possess potent immune functions, serving as sentinel cells to detect microbial infection through pattern recognition receptor activation and as antigen （cross）-presenting cells. LSECs cross-prime naive CD8 T cells, causing their rapid differentiation into memory T cells that relocate to secondary lymphoid tissues and provide protection when they re-encounter the antigen during microbial infection. Cross-presentation of viral antigens by LSECs derived from infected hepatocytes triggers local activation of effector CD8 T cells and thereby assures hepatic immune surveillance. The immune function of LSECs complements conventional immune-activating mechanisms to accommodate optimal immune surveillance against infectious microorganisms while preserving the integrity of the liver as a metabolic organ.
基金This paper was supported in part by a grant from Jilin Provincial Natural Science Foundation of China(20140520014JH)The 4th young scientist fund of Jilin University(2013068)
文摘Hepatitis B virus(HBV)infection is a major cause of chronic liver diseases that may progress to liver cirrhosis and hepatocellular carcinoma.Host immune responses are important factors that determine whether HBV infection is cleared or persists.After infection,viral replication occurs inside hepatocytes,and the secretion of infectious virions can take place at high rates for decades.Consequently,HBV DNA and viral proteins,like HBV early antigen(HBeAg)and HBV surface antigen(HBsAg),can be easily detected in serum.Chronic infection with HBV is the result of an ineffective antiviral immune response towards the virus.In this review,we discuss the role of immune cells in chronic HBV infection.
