To further investigate the osteogen ic potential of rabbit marrow stromal stem cells cultured in vitro. Methods: Rabbit marrow stromal stem cells were isolated by dens ity gradient centrifugation method and amplified ...To further investigate the osteogen ic potential of rabbit marrow stromal stem cells cultured in vitro. Methods: Rabbit marrow stromal stem cells were isolated by dens ity gradient centrifugation method and amplified in the flasks, using the osteog enic inducing conditions (OGC) as the culture media. The osteogenic potential of marrow stromal stem cells were investigated by means of bone seeking fluoresce nce (tetracycline) labelling, Alizarin red S (ARS) staining, Alcian blue Sirius red (AS) staining, and scanning electron microscope. Results: After being passaged, the marrow stromal stem cells in creased in number, became confluent and formed multi layer structure. The strom al stem cells excreted innumerable tiny granules, heaping up on the cell body an d merging gradually into foggy substances. These foggy substances kept on enlarg ing and formed round, oval, or flake like nodules. These nodules revealed brigh t golden yellow fluorescence under fluorescence microscope when labelled with te tracycline. Histochemical study with specific new bone staining with ARS reveale d positive calcium reaction, both denoting that they were newly formed bone tiss ues. After they were stained with AS, collagen and acid mucopolysaccharide were shown. Under scanning electron microscope, three types of cells with different c onfigurations were found. They were globular cells, spindle shaped cells and po lygonal or polygonal cells. Granules were excreted from the cells and heaped up on the cell body. Needle shaped and irregularly rectangular crystals also appea red and agglomerated with the granules to form nodules and trabecula like or fl ake like structures.Conclusions: Sequence of events of bone formation by rabbit mar row stromal stem cells cultured in vitro is fully depicted and confirmed, which provides the foundation for further investigating the mechanisms of osteoblast d ifferentiation from marrow stromal stem cells and the possible application in or thopaedics.展开更多
Polymers and polymeric composites have steadily reflected their importance in our daily life. Blending poly(vinyl alcohol) (PVA) with a potentially useful natural biopolymers such as hydroxypropyl cellulose (HPC) seem...Polymers and polymeric composites have steadily reflected their importance in our daily life. Blending poly(vinyl alcohol) (PVA) with a potentially useful natural biopolymers such as hydroxypropyl cellulose (HPC) seems to be an interesting way of preparing a polymeric blends. In the present work, blends of PVA/HPC of compositions (100/0, 90/10, 75/25, 50/50, 25/75, and 0/100 wt/wt%) were prepared to be used as bioequivalent materials. Thermal analyses [differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA)], and X-ray diffraction (XRD) were employed to characterize and reveal the miscibility map and the structural properties of such blend system. The obtained results of the thermal analyses showed variations in the glass transition temperature (Tg) indicating the miscibility of the blend systems. Moreover, the changes in the melting temperature (Tm), shape and area were attributed to the different degrees of crystallinity and the existence of polymer-polymer interactions between PVA and HPC molecules. The X-ray diffraction (XRD) analysis showed broadening and sharpening of peaks at different HPC concentrations with PVA. This indicated changes in the crystallinity/amorphosity ratio, and also suggested that the miscibility between the amorphous components of homo-polymers PVA and HPC is possible. The results showed that HPC doped in PVA film can improve the thermal stability of the film under investigation, leading to interesting technological applications.展开更多
Our study investigated preservation Undulate venus (</span></span><span><span><span style="font-family:""><i></span></span></span><span><sp...Our study investigated preservation Undulate venus (</span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">)</span></span></span><span><span><span style="font-family:""> by canning via three treatments </span></span></span><span><span><span style="font-family:"">that </span></span></span><span><span><span style="font-family:"">are canned raw, canned smoked and canned cooked as a healthy product with high nutritional value. Results<span> </span>reported that edible meat of </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:""> was free from Cu, Pb and Cd as a result of washing process. There was significantly (p < 0.05) higher in moisture content of canned raw compared with other tow treatments </span></span></span><span><span><span style="font-family:"">of </span></span></span><span><span><span style="font-family:"">canned smoked and canned cooked of </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">, and there was significantly reduction of protein content (p < 0.05) in tow treatments </span></span></span><span><span><span style="font-family:"">of </span></span></span><span><span><span style="font-family:"">canned smoked and canned cooked compared with the canned raw. Significant different (p < 0.05) in fat content </span></span></span><span><span><span style="font-family:"">w</span></span></span><span><span><span style="font-family:"">as canned raw <展开更多
The ＂soft canning＂ heat preservation technique is invented by Baoshan Iron ＆ Steel Co., Ltd. （Hereafter referred to Baosteel）, using the flexible insulation material which can be stuck on the surface of the heate...The ＂soft canning＂ heat preservation technique is invented by Baoshan Iron ＆ Steel Co., Ltd. （Hereafter referred to Baosteel）, using the flexible insulation material which can be stuck on the surface of the heated ingot or billet in the superalloy thermal process. This adhesive insulation material can reduce the temperature drop of the ingot or billet during the transferring or hot working process, and can deform with the billet without dropping. The surface temperature drop can be effectively reduced, so the products can be obtained a good surface quality and the structural uniformity can be improved. The technique is applied to hard-wrought superalloy forging of cogging, superalloy rods finished forging fire and superalloy hot die forging processing,and good results have been achieved.展开更多
文摘To further investigate the osteogen ic potential of rabbit marrow stromal stem cells cultured in vitro. Methods: Rabbit marrow stromal stem cells were isolated by dens ity gradient centrifugation method and amplified in the flasks, using the osteog enic inducing conditions (OGC) as the culture media. The osteogenic potential of marrow stromal stem cells were investigated by means of bone seeking fluoresce nce (tetracycline) labelling, Alizarin red S (ARS) staining, Alcian blue Sirius red (AS) staining, and scanning electron microscope. Results: After being passaged, the marrow stromal stem cells in creased in number, became confluent and formed multi layer structure. The strom al stem cells excreted innumerable tiny granules, heaping up on the cell body an d merging gradually into foggy substances. These foggy substances kept on enlarg ing and formed round, oval, or flake like nodules. These nodules revealed brigh t golden yellow fluorescence under fluorescence microscope when labelled with te tracycline. Histochemical study with specific new bone staining with ARS reveale d positive calcium reaction, both denoting that they were newly formed bone tiss ues. After they were stained with AS, collagen and acid mucopolysaccharide were shown. Under scanning electron microscope, three types of cells with different c onfigurations were found. They were globular cells, spindle shaped cells and po lygonal or polygonal cells. Granules were excreted from the cells and heaped up on the cell body. Needle shaped and irregularly rectangular crystals also appea red and agglomerated with the granules to form nodules and trabecula like or fl ake like structures.Conclusions: Sequence of events of bone formation by rabbit mar row stromal stem cells cultured in vitro is fully depicted and confirmed, which provides the foundation for further investigating the mechanisms of osteoblast d ifferentiation from marrow stromal stem cells and the possible application in or thopaedics.
文摘Polymers and polymeric composites have steadily reflected their importance in our daily life. Blending poly(vinyl alcohol) (PVA) with a potentially useful natural biopolymers such as hydroxypropyl cellulose (HPC) seems to be an interesting way of preparing a polymeric blends. In the present work, blends of PVA/HPC of compositions (100/0, 90/10, 75/25, 50/50, 25/75, and 0/100 wt/wt%) were prepared to be used as bioequivalent materials. Thermal analyses [differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA)], and X-ray diffraction (XRD) were employed to characterize and reveal the miscibility map and the structural properties of such blend system. The obtained results of the thermal analyses showed variations in the glass transition temperature (Tg) indicating the miscibility of the blend systems. Moreover, the changes in the melting temperature (Tm), shape and area were attributed to the different degrees of crystallinity and the existence of polymer-polymer interactions between PVA and HPC molecules. The X-ray diffraction (XRD) analysis showed broadening and sharpening of peaks at different HPC concentrations with PVA. This indicated changes in the crystallinity/amorphosity ratio, and also suggested that the miscibility between the amorphous components of homo-polymers PVA and HPC is possible. The results showed that HPC doped in PVA film can improve the thermal stability of the film under investigation, leading to interesting technological applications.
文摘Our study investigated preservation Undulate venus (</span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">)</span></span></span><span><span><span style="font-family:""> by canning via three treatments </span></span></span><span><span><span style="font-family:"">that </span></span></span><span><span><span style="font-family:"">are canned raw, canned smoked and canned cooked as a healthy product with high nutritional value. Results<span> </span>reported that edible meat of </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:""> was free from Cu, Pb and Cd as a result of washing process. There was significantly (p < 0.05) higher in moisture content of canned raw compared with other tow treatments </span></span></span><span><span><span style="font-family:"">of </span></span></span><span><span><span style="font-family:"">canned smoked and canned cooked of </span></span></span><span><span><span style="font-family:""><i></span></span></span><span><span><i><span style="font-family:"">Paphia undulate</span></i></span></span><span><span><i><span style="font-family:""></i></span></i></span></span><span><span><span style="font-family:"">, and there was significantly reduction of protein content (p < 0.05) in tow treatments </span></span></span><span><span><span style="font-family:"">of </span></span></span><span><span><span style="font-family:"">canned smoked and canned cooked compared with the canned raw. Significant different (p < 0.05) in fat content </span></span></span><span><span><span style="font-family:"">w</span></span></span><span><span><span style="font-family:"">as canned raw <
文摘The ＂soft canning＂ heat preservation technique is invented by Baoshan Iron ＆ Steel Co., Ltd. （Hereafter referred to Baosteel）, using the flexible insulation material which can be stuck on the surface of the heated ingot or billet in the superalloy thermal process. This adhesive insulation material can reduce the temperature drop of the ingot or billet during the transferring or hot working process, and can deform with the billet without dropping. The surface temperature drop can be effectively reduced, so the products can be obtained a good surface quality and the structural uniformity can be improved. The technique is applied to hard-wrought superalloy forging of cogging, superalloy rods finished forging fire and superalloy hot die forging processing,and good results have been achieved.
