[Objectives] To establish the quality standard of barberry branches. [Methods] Microscopic identification and thin layer identification were used to qualitatively identify barberry branches. Berberine content was dete...[Objectives] To establish the quality standard of barberry branches. [Methods] Microscopic identification and thin layer identification were used to qualitatively identify barberry branches. Berberine content was determined by HPLC method, and the content of water, total ash, acid insoluble ash and extract was detected according to the method of the Chinese Pharmacopoeia (2020 edition). [Results] The microscopic identification showed that the features were obvious, and stone cells, cork cells, epidermal cells, stomata, fibers and catheter with reticulated pores could be found. Berberine was detected in barberry branches by thin layer chromatography, and the characteristic spots were separated clearly. Moisture, total ash, and acid insoluble ash content shall not exceed 13%, 6%, and 3%, respectively, and extract content shall not be less than 10%. Berberine hydrochloride (C_(20)H_(18)ClNO_(4)) should be calculated in the branches of Berberis wilsonae Hemsley and Berberis aggregata C. K. Schneid., and berberine (C_(20)H_(17)NO_(4)) content should not be less than 0.05%. The linear relationship was good in the range of 0.002-0.240 mg/mL ( R^(2)=0.999 5). The average recovery was 89.63%, and RSD was 5.28%. [Conclusions] The method was simple, accurate and reproducible, and can be used for the quality control of barberry branches.展开更多
基金Supported by the National Key Research and Development Plan(2018YFC-06101)Sichuan Provincial Natural Science Foundation(2022NSFSC1605)+2 种基金Traditional Chinese Medicine(Ethnic Medicine)Standard Improvement of Sichuan Medical Products Administration(510201202102305)Leading Talents Support Plan of National Ethnic Affairs Commission in 2022Special Project for the University-Level Innovation Team in the Fundamental Research Funds for the Central Universities(ZYN2022067).
文摘[Objectives] To establish the quality standard of barberry branches. [Methods] Microscopic identification and thin layer identification were used to qualitatively identify barberry branches. Berberine content was determined by HPLC method, and the content of water, total ash, acid insoluble ash and extract was detected according to the method of the Chinese Pharmacopoeia (2020 edition). [Results] The microscopic identification showed that the features were obvious, and stone cells, cork cells, epidermal cells, stomata, fibers and catheter with reticulated pores could be found. Berberine was detected in barberry branches by thin layer chromatography, and the characteristic spots were separated clearly. Moisture, total ash, and acid insoluble ash content shall not exceed 13%, 6%, and 3%, respectively, and extract content shall not be less than 10%. Berberine hydrochloride (C_(20)H_(18)ClNO_(4)) should be calculated in the branches of Berberis wilsonae Hemsley and Berberis aggregata C. K. Schneid., and berberine (C_(20)H_(17)NO_(4)) content should not be less than 0.05%. The linear relationship was good in the range of 0.002-0.240 mg/mL ( R^(2)=0.999 5). The average recovery was 89.63%, and RSD was 5.28%. [Conclusions] The method was simple, accurate and reproducible, and can be used for the quality control of barberry branches.
