Purpose The purpose of this study is to review the urine products of bone breakdown as markers of bone resorption and usefulness of urinary hydroxyproline.Data Related researches published in 1985 -2000 were systemati...Purpose The purpose of this study is to review the urine products of bone breakdown as markers of bone resorption and usefulness of urinary hydroxyproline.Data Related researches published in 1985 -2000 were systematically reviewed.Results Bone markers could be used for early diagnosis of bone metabolic diseases. Biochemical markers of bone resorption that reflect osteoclast activity and/or collagen degradation provide a new and potentially important clinical tool for the assessment and monitoring of bone metabolism. Assessment of bone resorption can be achieved with measurement of urinary hydroxylysine glycosides, urinary excretion of the collagen pyridinium cross-links, urinary excretion of type I collagen telopeptide breakdown products (cross-linked telopeptides) and urinary hydroxyproline.Conclusion Urinary hydroxyproline has been in use as a marker of bone resorption, but it lacks sensitivity and specificity. It is a modified aminoacid that is a metabolic product of collagen breakdown. Hydroxyproline may be released either free or with fragments of the collagen molecule attached during bone resorption, and it is also liberated by the breakdown of complement and nonskeletal collagen.展开更多
目的:探讨I型胶原交联羧基末端肽(pyridinoline cross-linked C-telopeptides of type I collagen,I CTP)和骨唾液酸蛋白(bone s ialoprotein,BSP)在甲状腺癌骨转移早期诊断中的临床价值。方法:将确诊的甲状腺癌患者1 0 2例,分为无骨转...目的:探讨I型胶原交联羧基末端肽(pyridinoline cross-linked C-telopeptides of type I collagen,I CTP)和骨唾液酸蛋白(bone s ialoprotein,BSP)在甲状腺癌骨转移早期诊断中的临床价值。方法:将确诊的甲状腺癌患者1 0 2例,分为无骨转移组和骨转移组,采用So l owa y分级将骨转移组病例分为4级(0~I I I级),ELI SA测定血清I CTP和BSP水平。半年后再次评估骨转移情况。结果:骨转移组血清I CTP和BSP水平明显高于无骨转移组及健康对照组(P<0.05)。骨转移组中随着转移病灶数目的增加,血清I CTP和BSP水平逐步升高,与病情进展呈显著正相关(P<0.01)。受试者工作特征曲线(receiver operating curve,ROC)分析显示:血清I CTP诊断甲状腺癌骨转移截断值为18 U/L,灵敏度85.2%,特异度98.9%,ROC曲线下面积0.967;血清BSP诊断甲状腺癌骨转移截断值为2.8 g/L,灵敏度87.9%,特异度99.1%,ROC曲线下面积0.976;初诊骨转移组和新发骨转移组的I CTP和BSP水平均显著高于无骨转移组(P<0.05)。结论:血清I CTP和BSP可用作诊断甲状腺癌骨转移的参考指标,通过对二者水平的监测可预测骨转移发生风险。展开更多
目的探讨骨形成指标I型胶原氨基端肽原(procollagen type I N-terminal propeptide,PINP)与骨吸收指标β-异构C-端肽(beta-isomerized C-telopeptide,β-CTX)的表达在2型糖尿病(type 2 diabetes,T2DM)合并骨质疏松(osteoprosis,OP)中的...目的探讨骨形成指标I型胶原氨基端肽原(procollagen type I N-terminal propeptide,PINP)与骨吸收指标β-异构C-端肽(beta-isomerized C-telopeptide,β-CTX)的表达在2型糖尿病(type 2 diabetes,T2DM)合并骨质疏松(osteoprosis,OP)中的意义。方法将118例研究对象按双能X线检测骨密度(bone mineral density,BMD)的检查结果分为3组,骨量正常组41例,骨量减少组42例,骨质疏松组35例。记录其性别、年龄、身高、体重、体质指数(body mass index,BMI)、糖尿病病程等一般基线资料。使用全自动生化分析仪测定空腹血糖(fasting blood glucose,FBG)、血钙(blood calcium,Ca)、血磷(phosphate,P)、血清胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、碱性磷酸酶(alkaline phosphatase,ALP)、甲状旁腺素(parathyroid hormone,PTH)等生化指标,高压液相法测定糖化血红蛋白(Hb A1c),ELISA法检测血清骨形成指标PINP及血清骨吸收指标β-CTX并进行组间比较。结果与骨量正常组比较,骨量减少组与OP组中的PINP、β-CTX均高于正常组(P<0.05),与骨量减少组相比,OP组中PINP、β-CTX均高于减少组(P<0.05)。股骨颈BMD与年龄、病程、FPG、Hb A1c、β-CTX、PINP呈负相关(r=-0.559、-0.368、-0.255、-0.284、-0.613、-0.314,P<0.05),与BMI无明显相关性(P>0.05)。结论 T2DM合并OP患者中骨形成与骨吸收均增高,属于高转换性的骨代谢,监测骨代谢标志物PINP与β-CTX,有助于早期诊断及防治T2DM合并OP的发生与发展。展开更多
目的:观察"短刺法"对兔膝骨关节炎(osteoarthritis of the knee,KOA)软骨中Ⅱ型胶原及分子标记物Ⅱ型前胶原羧基端前肽(pro-collagen typeⅡC-terminal propeptide,PⅡCP)、Ⅱ型胶原羧基端端肽(C-telopeptide of typeⅡcollag...目的:观察"短刺法"对兔膝骨关节炎(osteoarthritis of the knee,KOA)软骨中Ⅱ型胶原及分子标记物Ⅱ型前胶原羧基端前肽(pro-collagen typeⅡC-terminal propeptide,PⅡCP)、Ⅱ型胶原羧基端端肽(C-telopeptide of typeⅡcollagen,CTX-Ⅱ)表达的影响。方法:40只新西兰兔取10只作为正常组,剩余30只复制KOA模型,X线检测评估造模结果。造模成功的新西兰兔随机分为模型组、短刺组和普通刺法组,每组10只。短刺组和普通刺法组均取"内膝眼""犊鼻""阴陵泉""足三里""梁丘"穴,短刺组采用贴骨进针,针刺深度以抵骨为准,普通刺法组常规针刺,除"梁丘"外均连接电针仪,每次20min,每日1次,5次为一疗程,疗程间休息2d,连续治疗4个疗程。正常组与模型组相同固定。治疗结束后采用磁共振成像(magnetic resonance imaging,MRI)进行膝关节影像学观察;透射电镜观察膝关节软骨细胞组织结构;HE染色观察膝关节病理学改变;TUNEL法检测膝关节软骨细胞凋亡情况;免疫印迹法(Western-blot)和逆转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)检测Ⅱ型胶原蛋白和mRNA表达;酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测PⅡCP、CTX-Ⅱ表达。结果:治疗结束后,与模型组相比,短刺组与普通刺法组关节腔积液减少,无软骨下骨水肿;膝关节软骨细胞形态规则,凋亡减少;Ⅱ型胶原蛋白和mRNA表达显著升高(均P<0.05);PⅡCP水平上升(均P<0.05),而CTX-Ⅱ水平下降(均P<0.05)。短刺组Ⅱ型胶原蛋白及mRNA表达、PⅡCP、CTX-Ⅱ水平与普通刺法组比较差异均有统计学意义(均P<0.05);短刺组与正常组比较差异无统计学意义(均P>0.05)。结论:"短刺法"能显著改善膝骨关节炎关节软骨的病理状态,减少软骨细胞凋亡,可能通过调节PⅡCP、CTX-Ⅱ表达来促进Ⅱ型胶原的合成,且效果优于普通针刺。展开更多
文摘Purpose The purpose of this study is to review the urine products of bone breakdown as markers of bone resorption and usefulness of urinary hydroxyproline.Data Related researches published in 1985 -2000 were systematically reviewed.Results Bone markers could be used for early diagnosis of bone metabolic diseases. Biochemical markers of bone resorption that reflect osteoclast activity and/or collagen degradation provide a new and potentially important clinical tool for the assessment and monitoring of bone metabolism. Assessment of bone resorption can be achieved with measurement of urinary hydroxylysine glycosides, urinary excretion of the collagen pyridinium cross-links, urinary excretion of type I collagen telopeptide breakdown products (cross-linked telopeptides) and urinary hydroxyproline.Conclusion Urinary hydroxyproline has been in use as a marker of bone resorption, but it lacks sensitivity and specificity. It is a modified aminoacid that is a metabolic product of collagen breakdown. Hydroxyproline may be released either free or with fragments of the collagen molecule attached during bone resorption, and it is also liberated by the breakdown of complement and nonskeletal collagen.
文摘目的:探讨I型胶原交联羧基末端肽(pyridinoline cross-linked C-telopeptides of type I collagen,I CTP)和骨唾液酸蛋白(bone s ialoprotein,BSP)在甲状腺癌骨转移早期诊断中的临床价值。方法:将确诊的甲状腺癌患者1 0 2例,分为无骨转移组和骨转移组,采用So l owa y分级将骨转移组病例分为4级(0~I I I级),ELI SA测定血清I CTP和BSP水平。半年后再次评估骨转移情况。结果:骨转移组血清I CTP和BSP水平明显高于无骨转移组及健康对照组(P<0.05)。骨转移组中随着转移病灶数目的增加,血清I CTP和BSP水平逐步升高,与病情进展呈显著正相关(P<0.01)。受试者工作特征曲线(receiver operating curve,ROC)分析显示:血清I CTP诊断甲状腺癌骨转移截断值为18 U/L,灵敏度85.2%,特异度98.9%,ROC曲线下面积0.967;血清BSP诊断甲状腺癌骨转移截断值为2.8 g/L,灵敏度87.9%,特异度99.1%,ROC曲线下面积0.976;初诊骨转移组和新发骨转移组的I CTP和BSP水平均显著高于无骨转移组(P<0.05)。结论:血清I CTP和BSP可用作诊断甲状腺癌骨转移的参考指标,通过对二者水平的监测可预测骨转移发生风险。
文摘目的:观察"短刺法"对兔膝骨关节炎(osteoarthritis of the knee,KOA)软骨中Ⅱ型胶原及分子标记物Ⅱ型前胶原羧基端前肽(pro-collagen typeⅡC-terminal propeptide,PⅡCP)、Ⅱ型胶原羧基端端肽(C-telopeptide of typeⅡcollagen,CTX-Ⅱ)表达的影响。方法:40只新西兰兔取10只作为正常组,剩余30只复制KOA模型,X线检测评估造模结果。造模成功的新西兰兔随机分为模型组、短刺组和普通刺法组,每组10只。短刺组和普通刺法组均取"内膝眼""犊鼻""阴陵泉""足三里""梁丘"穴,短刺组采用贴骨进针,针刺深度以抵骨为准,普通刺法组常规针刺,除"梁丘"外均连接电针仪,每次20min,每日1次,5次为一疗程,疗程间休息2d,连续治疗4个疗程。正常组与模型组相同固定。治疗结束后采用磁共振成像(magnetic resonance imaging,MRI)进行膝关节影像学观察;透射电镜观察膝关节软骨细胞组织结构;HE染色观察膝关节病理学改变;TUNEL法检测膝关节软骨细胞凋亡情况;免疫印迹法(Western-blot)和逆转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)检测Ⅱ型胶原蛋白和mRNA表达;酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测PⅡCP、CTX-Ⅱ表达。结果:治疗结束后,与模型组相比,短刺组与普通刺法组关节腔积液减少,无软骨下骨水肿;膝关节软骨细胞形态规则,凋亡减少;Ⅱ型胶原蛋白和mRNA表达显著升高(均P<0.05);PⅡCP水平上升(均P<0.05),而CTX-Ⅱ水平下降(均P<0.05)。短刺组Ⅱ型胶原蛋白及mRNA表达、PⅡCP、CTX-Ⅱ水平与普通刺法组比较差异均有统计学意义(均P<0.05);短刺组与正常组比较差异无统计学意义(均P>0.05)。结论:"短刺法"能显著改善膝骨关节炎关节软骨的病理状态,减少软骨细胞凋亡,可能通过调节PⅡCP、CTX-Ⅱ表达来促进Ⅱ型胶原的合成,且效果优于普通针刺。
