The sequences which consist of any segment of a chaos sequence are called asC-sequences. These sequences could be used as a kind of input signals to replace M-sequences in theprocess identification. This substitution ...The sequences which consist of any segment of a chaos sequence are called asC-sequences. These sequences could be used as a kind of input signals to replace M-sequences in theprocess identification. This substitution is theoretically proved to be feasible. InverseC-sequences are created in a way similar to inverse M-sequences to solve the problem thatC-sequences have non-ideal balance property, that is, the numbers of '0' and '1' are unequal.Besides its good pseudo-random property, the sequences have other advantages such as easy togenerate, varieties of the segment and adjustable cycle time.展开更多
Following spinal cord ischemia/reperfusion injury,an endogenous damage system is immediately activated and participates in a cascade reaction.It is difficult to interpret dynamic changes in these pathways,but the exam...Following spinal cord ischemia/reperfusion injury,an endogenous damage system is immediately activated and participates in a cascade reaction.It is difficult to interpret dynamic changes in these pathways,but the examination of the transcriptome may provide some information.The transcriptome reflects highly dynamic genomic and genetic information and can be seen as a precursor for the proteome.We used DNA microarrays to measure the expression levels of dynamic evolution-related m RNA after spinal cord ischemia/reperfusion injury in rats.The abdominal aorta was blocked with a vascular clamp for 90 minutes and underwent reperfusion for 24 and 48 hours.The simple ischemia group and sham group served as controls.After rats had regained consciousness,hindlimbs showed varying degrees of functional impairment,and gradually improved with prolonged reperfusion in spinal cord ischemia/reperfusion injury groups.Hematoxylin-eosin staining demonstrated that neuronal injury and tissue edema were most severe in the 24-hour reperfusion group,and mitigated in the 48-hour reperfusion group.There were 8,242 differentially expressed m RNAs obtained by Multi-Class Dif in the simple ischemia group,24-hour and 48-hour reperfusion groups.Sixteen m RNA dynamic expression patterns were obtained by Serial Test Cluster.Of them,five patterns were significant.In the No.28 pattern,all differential genes were detected in the 24-hour reperfusion group,and their expressions showed a trend in up-regulation.No.11 pattern showed a decreasing trend in m RNA whereas No.40 pattern showed an increasing trend in m RNA from ischemia to 48 hours of reperfusion,and peaked at 48 hours.In the No.25 and No.27 patterns,differential expression appeared only in the 24-hour and 48-hour reperfusion groups.Among the five m RNA dynamic expression patterns,No.11 and No.40 patterns could distinguish normal spinal cord from pathological tissue.No.25 and No.27 patterns could distinguish simple ischemia from ischemia/reperfusion.No.28 pattern could analyze the need for in展开更多
通过测定重组水蛭素Ⅲ的质粒中目的基因序列、重组水蛭素Ⅲ分子质量、C末端序列以及菌体蛋白和DNA残留量,对重组水蛭素Ⅲ的质量进行研究。结果:重组质粒经NheⅠ、HindⅢ双酶切和基因序列测定,表明工程菌所携带的质粒上含有完整的信号肽...通过测定重组水蛭素Ⅲ的质粒中目的基因序列、重组水蛭素Ⅲ分子质量、C末端序列以及菌体蛋白和DNA残留量,对重组水蛭素Ⅲ的质量进行研究。结果:重组质粒经NheⅠ、HindⅢ双酶切和基因序列测定,表明工程菌所携带的质粒上含有完整的信号肽基因、NheⅠ酶切位点、水蛭素HV3基因和HindⅢ酶切位点;还原性SDS PAGE结果表明在14 000 u处有单一条带,为重组水蛭素Ⅲ二聚体;测定样品分子C端序列为E D YA D E P I P E F,与重组水蛭素Ⅲ的理论值一致;重组水蛭素Ⅲ样品中的残留菌体蛋白和DNA含量分别是0.02%和每剂量样品中残留DNA含量低于2.8 ng。结论:从基因水平、蛋白水平和杂质的角度证明重组水蛭素Ⅲ产品符合质量控制标准。展开更多
基金This work was financially supported by the National Natural Science Foundation of China (No.70071047, No.10247005)the Postdoctoral Science Foundation of China (No.2002032129)
文摘The sequences which consist of any segment of a chaos sequence are called asC-sequences. These sequences could be used as a kind of input signals to replace M-sequences in theprocess identification. This substitution is theoretically proved to be feasible. InverseC-sequences are created in a way similar to inverse M-sequences to solve the problem thatC-sequences have non-ideal balance property, that is, the numbers of '0' and '1' are unequal.Besides its good pseudo-random property, the sequences have other advantages such as easy togenerate, varieties of the segment and adjustable cycle time.
基金supported by the National Natural Science Foundation of China,No.81350013 and 31572217
文摘Following spinal cord ischemia/reperfusion injury,an endogenous damage system is immediately activated and participates in a cascade reaction.It is difficult to interpret dynamic changes in these pathways,but the examination of the transcriptome may provide some information.The transcriptome reflects highly dynamic genomic and genetic information and can be seen as a precursor for the proteome.We used DNA microarrays to measure the expression levels of dynamic evolution-related m RNA after spinal cord ischemia/reperfusion injury in rats.The abdominal aorta was blocked with a vascular clamp for 90 minutes and underwent reperfusion for 24 and 48 hours.The simple ischemia group and sham group served as controls.After rats had regained consciousness,hindlimbs showed varying degrees of functional impairment,and gradually improved with prolonged reperfusion in spinal cord ischemia/reperfusion injury groups.Hematoxylin-eosin staining demonstrated that neuronal injury and tissue edema were most severe in the 24-hour reperfusion group,and mitigated in the 48-hour reperfusion group.There were 8,242 differentially expressed m RNAs obtained by Multi-Class Dif in the simple ischemia group,24-hour and 48-hour reperfusion groups.Sixteen m RNA dynamic expression patterns were obtained by Serial Test Cluster.Of them,five patterns were significant.In the No.28 pattern,all differential genes were detected in the 24-hour reperfusion group,and their expressions showed a trend in up-regulation.No.11 pattern showed a decreasing trend in m RNA whereas No.40 pattern showed an increasing trend in m RNA from ischemia to 48 hours of reperfusion,and peaked at 48 hours.In the No.25 and No.27 patterns,differential expression appeared only in the 24-hour and 48-hour reperfusion groups.Among the five m RNA dynamic expression patterns,No.11 and No.40 patterns could distinguish normal spinal cord from pathological tissue.No.25 and No.27 patterns could distinguish simple ischemia from ischemia/reperfusion.No.28 pattern could analyze the need for in
文摘通过测定重组水蛭素Ⅲ的质粒中目的基因序列、重组水蛭素Ⅲ分子质量、C末端序列以及菌体蛋白和DNA残留量,对重组水蛭素Ⅲ的质量进行研究。结果:重组质粒经NheⅠ、HindⅢ双酶切和基因序列测定,表明工程菌所携带的质粒上含有完整的信号肽基因、NheⅠ酶切位点、水蛭素HV3基因和HindⅢ酶切位点;还原性SDS PAGE结果表明在14 000 u处有单一条带,为重组水蛭素Ⅲ二聚体;测定样品分子C端序列为E D YA D E P I P E F,与重组水蛭素Ⅲ的理论值一致;重组水蛭素Ⅲ样品中的残留菌体蛋白和DNA含量分别是0.02%和每剂量样品中残留DNA含量低于2.8 ng。结论:从基因水平、蛋白水平和杂质的角度证明重组水蛭素Ⅲ产品符合质量控制标准。
