Classification of the Asian snake genera Lycodon and Oligodon has proven challenging. We conducted a molecular phylogenetic analysis to estimate the phylogenetic relationships in the genus of Lycodon and clarify the t...Classification of the Asian snake genera Lycodon and Oligodon has proven challenging. We conducted a molecular phylogenetic analysis to estimate the phylogenetic relationships in the genus of Lycodon and clarify the taxonomic status of Oligodon multizonatum using mitochondrial（cyt b, ND4） and nuclear（c-mos） genes. Phylogenetic trees estimated using Maximum Likelihood and Bayesian Inference indicated that O. multizonatum is actually a species of Lycodon. Comparing morphological data from O. multizonatum and its closest relatives also supported this conclusion. Our results imply that a thorough review of the evolutionary relationships in the genus of Lycodon is strong suggested.展开更多
Aim： To immunolocalize the c-mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development. Methods： Semi-quantitative reverse transcription-polymerase chain re...Aim： To immunolocalize the c-mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development. Methods： Semi-quantitative reverse transcription-polymerase chain reaction （RT- PCR） and in situ hybridization techniques were used to examine c-mos mRNA and indirect immunofluorescence was used to localize c-Mos protein in mouse testis on postnatal days 14, 21, 25, 28, 30, 35, 49 and 70. Results： c-mos mRNA remained low on postnatal days 14-21, increased abruptly from day 25 and peaked on day 30. Its levels decreased a little on day 35 and became almost stable thereafter until day 70. c-mos mRNA was localized in the nucleus and cytoplasm of the spermatocytes and round spermatids. The nuclear staining was much stronger than the cytoplasmic staining. Using a polyclonal anti-c-Mos antibody, Western blotting detected a single band at 43 kDa in testis lysate, c-Mos protein was exclusively localized to the elongating spermatids and was first detected on postnatal day 30. The number of c-Mos-positive spermatids increased progressively till day 49 and stabilized thereafter. Conclusion： The c-mos gene displays a spatial and temporal expression pattern in the mouse testis during postnatal development at both the mRNA and protein level. This suggests that c-mos might play important roles in spermatogenesis. （Asian J Androl 2008 Mar; 10： 277-285）展开更多
We conducted experiments of oversensing generation of pacemaker (PM) and X-irradiation direction dependency of PM, and examined the oversensing suppression method, using 8 different types of PMs. It was found out from...We conducted experiments of oversensing generation of pacemaker (PM) and X-irradiation direction dependency of PM, and examined the oversensing suppression method, using 8 different types of PMs. It was found out from this experiment that oversensing would occur when some conditions (X-irradiation direction, X-irradiation intensity) are met. Oversensing occurred with the most low irradiation conditions (kV × mA) when PM was irradiated at 90° (vertically to C-MOS;Complementary Metal Oxide Semiconductor). The acuter the angle of irradiation is (α > 90° < α), the higher the irradiation conditions (kV × mA) at which oversensing start to occur. In plain X-ray photography, oversensing was confirmed under the irradiation conditions of (cervical spine, thoracic spine, lateral thoracic spine, rib, shoulder joint, collarbone, humerus, and chest).Once the irradiation angle and irradiation conditions (kV × mA) are available, oversensing is predictable to some extent. Our findings will help to predict oversensing generation of plain X-ray photography and suppress oversensing. Oversensing can be suppressed in most of the radiography by lowering tube current to 100 mA, but a 1.0 mm High-Density Tungsten Sheet must be put on PM in high tube voltage radiography.展开更多
Among different endangered animal species,snakes are the most neglected creature looked at with apathy and therefore,are ruthlessly killed,illegally trafficked,and poached for their venom,lucrative skin,meat,and bones...Among different endangered animal species,snakes are the most neglected creature looked at with apathy and therefore,are ruthlessly killed,illegally trafficked,and poached for their venom,lucrative skin,meat,and bones for manufacturing of medicines,accessories,and food items.Establishing the identity of the endangered snake species is important for punishing the offenders under Wildlife Protection Act(WPA)(1972)but morphological characters fail to establish identity as they are often altered.The technique of identification of snake species at molecular level holds very effective conclusion in punishing offender.Here,we have constructed and demonstrated a novel multiplexing polymerase chain reaction technique,using 16S rRNA and C-mos gene for identification of four Indian snake species,namely Ptyas mucosa,Daboia russellii,Naja naja,and Xenochrophis piscator.They are listed in Appendix-II and III of convention on international trade in endangered species of wild fauna and flora and Schedule II;Part II of Indian WPA,1972.Therefore,it may be considered a functional tool for establishing species-specific identity of four Indian snake species and promising to be useful for their conservation.展开更多
In order to effectively utilize the magnetic-dielectric synergy and interfacial engineering,in this paper,yolk–shell structured magnetic multicomponent nanocomposites(MCNCs)including CoNi@void@C and CoNi@void@C@MoS_(...In order to effectively utilize the magnetic-dielectric synergy and interfacial engineering,in this paper,yolk–shell structured magnetic multicomponent nanocomposites(MCNCs)including CoNi@void@C and CoNi@void@C@MoS_(2) were produced in large scale by in-situ pyrolysis of cubic CoNi Prussian blue analogs(PBAs)followed by the hydrothermal process,respectively.Because of their unique structures,excellent synergistic effect between dielectric and magnetic loss,the as-prepared CoNi@void@C and CoNi@void@C@MoS_(2) MCNCs displayed very outstanding electromagnetic wave absorption performances(EMWAPs)including strong absorption capabilities,broad absorption bandwidth and thin matching thicknesses.Furthermore,the as-prepared CoNi@void@C and CoNi@void@C@MoS_(2) MCNCs well maintained the cubic configuration of CoNi PBAs even after the thermal treatment and hydrothermal processes.The unique structure and formed carbon layers effectively prevented the corrosion of internal CoNi alloy during the formation of MoS_(2),and CoNi@void@C@MoS_(2) MCNCs with different MoS_(2) contents could be synthesized by controlling the hydrothermal temperature.The obtained results revealed that the EM parameters,dielectric and magnetic loss capabilities of CoNi@void@C@MoS_(2) MCNCs could be tuned by controlling hydrothermal temperature and filler loading,which made their outstanding EMWAPs could be achieved in different frequency regions.Taking account of simple process,low density and high chemical stability,our findings provided a new and effective pathway to develop the strong wideband microwave absorbers.展开更多
基金supported by the National Natural Science Foundation of China (NSFC 31071913)
文摘Classification of the Asian snake genera Lycodon and Oligodon has proven challenging. We conducted a molecular phylogenetic analysis to estimate the phylogenetic relationships in the genus of Lycodon and clarify the taxonomic status of Oligodon multizonatum using mitochondrial（cyt b, ND4） and nuclear（c-mos） genes. Phylogenetic trees estimated using Maximum Likelihood and Bayesian Inference indicated that O. multizonatum is actually a species of Lycodon. Comparing morphological data from O. multizonatum and its closest relatives also supported this conclusion. Our results imply that a thorough review of the evolutionary relationships in the genus of Lycodon is strong suggested.
文摘Aim： To immunolocalize the c-mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development. Methods： Semi-quantitative reverse transcription-polymerase chain reaction （RT- PCR） and in situ hybridization techniques were used to examine c-mos mRNA and indirect immunofluorescence was used to localize c-Mos protein in mouse testis on postnatal days 14, 21, 25, 28, 30, 35, 49 and 70. Results： c-mos mRNA remained low on postnatal days 14-21, increased abruptly from day 25 and peaked on day 30. Its levels decreased a little on day 35 and became almost stable thereafter until day 70. c-mos mRNA was localized in the nucleus and cytoplasm of the spermatocytes and round spermatids. The nuclear staining was much stronger than the cytoplasmic staining. Using a polyclonal anti-c-Mos antibody, Western blotting detected a single band at 43 kDa in testis lysate, c-Mos protein was exclusively localized to the elongating spermatids and was first detected on postnatal day 30. The number of c-Mos-positive spermatids increased progressively till day 49 and stabilized thereafter. Conclusion： The c-mos gene displays a spatial and temporal expression pattern in the mouse testis during postnatal development at both the mRNA and protein level. This suggests that c-mos might play important roles in spermatogenesis. （Asian J Androl 2008 Mar; 10： 277-285）
文摘We conducted experiments of oversensing generation of pacemaker (PM) and X-irradiation direction dependency of PM, and examined the oversensing suppression method, using 8 different types of PMs. It was found out from this experiment that oversensing would occur when some conditions (X-irradiation direction, X-irradiation intensity) are met. Oversensing occurred with the most low irradiation conditions (kV × mA) when PM was irradiated at 90° (vertically to C-MOS;Complementary Metal Oxide Semiconductor). The acuter the angle of irradiation is (α > 90° < α), the higher the irradiation conditions (kV × mA) at which oversensing start to occur. In plain X-ray photography, oversensing was confirmed under the irradiation conditions of (cervical spine, thoracic spine, lateral thoracic spine, rib, shoulder joint, collarbone, humerus, and chest).Once the irradiation angle and irradiation conditions (kV × mA) are available, oversensing is predictable to some extent. Our findings will help to predict oversensing generation of plain X-ray photography and suppress oversensing. Oversensing can be suppressed in most of the radiography by lowering tube current to 100 mA, but a 1.0 mm High-Density Tungsten Sheet must be put on PM in high tube voltage radiography.
文摘Among different endangered animal species,snakes are the most neglected creature looked at with apathy and therefore,are ruthlessly killed,illegally trafficked,and poached for their venom,lucrative skin,meat,and bones for manufacturing of medicines,accessories,and food items.Establishing the identity of the endangered snake species is important for punishing the offenders under Wildlife Protection Act(WPA)(1972)but morphological characters fail to establish identity as they are often altered.The technique of identification of snake species at molecular level holds very effective conclusion in punishing offender.Here,we have constructed and demonstrated a novel multiplexing polymerase chain reaction technique,using 16S rRNA and C-mos gene for identification of four Indian snake species,namely Ptyas mucosa,Daboia russellii,Naja naja,and Xenochrophis piscator.They are listed in Appendix-II and III of convention on international trade in endangered species of wild fauna and flora and Schedule II;Part II of Indian WPA,1972.Therefore,it may be considered a functional tool for establishing species-specific identity of four Indian snake species and promising to be useful for their conservation.
基金supported by the Fund of Fok Ying Tung Education Foundation,the Natural Science Foundation of Guizhou province(No.2017-1034)the Major Research Project of innovative Group of Guizhou province(No.2018-013)+1 种基金the National Natural Science Foundation of China(Nos.11604060,52101010 and 11964006)the Foundation of the National Key Project for Basic Research(No.2012CB932304)for financial support.
文摘In order to effectively utilize the magnetic-dielectric synergy and interfacial engineering,in this paper,yolk–shell structured magnetic multicomponent nanocomposites(MCNCs)including CoNi@void@C and CoNi@void@C@MoS_(2) were produced in large scale by in-situ pyrolysis of cubic CoNi Prussian blue analogs(PBAs)followed by the hydrothermal process,respectively.Because of their unique structures,excellent synergistic effect between dielectric and magnetic loss,the as-prepared CoNi@void@C and CoNi@void@C@MoS_(2) MCNCs displayed very outstanding electromagnetic wave absorption performances(EMWAPs)including strong absorption capabilities,broad absorption bandwidth and thin matching thicknesses.Furthermore,the as-prepared CoNi@void@C and CoNi@void@C@MoS_(2) MCNCs well maintained the cubic configuration of CoNi PBAs even after the thermal treatment and hydrothermal processes.The unique structure and formed carbon layers effectively prevented the corrosion of internal CoNi alloy during the formation of MoS_(2),and CoNi@void@C@MoS_(2) MCNCs with different MoS_(2) contents could be synthesized by controlling the hydrothermal temperature.The obtained results revealed that the EM parameters,dielectric and magnetic loss capabilities of CoNi@void@C@MoS_(2) MCNCs could be tuned by controlling hydrothermal temperature and filler loading,which made their outstanding EMWAPs could be achieved in different frequency regions.Taking account of simple process,low density and high chemical stability,our findings provided a new and effective pathway to develop the strong wideband microwave absorbers.
