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短短芽胞杆菌FJAT-0809-GLX菌剂的制备及其对枇杷保鲜效果的研究 被引量:14
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作者 车建美 郑雪芳 +2 位作者 刘波 苏明星 朱育菁 《保鲜与加工》 CAS 2011年第5期6-9,共4页
将2‰的琼脂和2%~5%的NaCl添加到具有保鲜功能的微生物短短芽胞杆菌FJAT-0809-GLX发酵液中,制成微生物保鲜菌胶悬剂,其活菌含量高,质地均匀,无上下分层,稳定性能良好。将其应用于枇杷保鲜试验的结果表明,该微生物保鲜菌制剂对"早... 将2‰的琼脂和2%~5%的NaCl添加到具有保鲜功能的微生物短短芽胞杆菌FJAT-0809-GLX发酵液中,制成微生物保鲜菌胶悬剂,其活菌含量高,质地均匀,无上下分层,稳定性能良好。将其应用于枇杷保鲜试验的结果表明,该微生物保鲜菌制剂对"早钟六号"和"解放钟"枇杷果实具有良好的保鲜效果,在室温条件下(20~25℃)贮藏8 d时,与对照相比,可显著降低失重率,提高好果率。该制剂经动物急性毒性检测分析结果为无毒级。 展开更多
关键词 短短芽胞杆菌 胶悬剂 枇杷 可溶性固形物 失重率 好果率
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响应面法优化短短芽胞杆菌ch2-22的发酵工艺 被引量:6
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作者 孙文 刘倩倩 +4 位作者 胡彦婷 王雅娜 程辉彩 张根伟 张丽萍 《微生物学杂志》 CAS CSCD 2017年第6期62-69,共8页
在摇瓶发酵条件下,优化提高短短芽胞杆菌ch2-22芽胞浓度和抑菌活性的发酵培养基和培养条件。首先在单因素试验基础上进行响应面设计对ch2-22的发酵培养基进行优化,然后使用单因素试验方法确定最佳发酵条件,得到优化发酵培养基为淀粉35.0... 在摇瓶发酵条件下,优化提高短短芽胞杆菌ch2-22芽胞浓度和抑菌活性的发酵培养基和培养条件。首先在单因素试验基础上进行响应面设计对ch2-22的发酵培养基进行优化,然后使用单因素试验方法确定最佳发酵条件,得到优化发酵培养基为淀粉35.05 g/L,豆饼粉26.08 g/L,蔗糖10 g/L,鱼粉5 g/L,Na Cl 1 g/L,Mg SO40.3 g/L,(NH4)2SO43 g/L,Mn SO40.1 g/L,K2HPO43 g/L,酵母膏1 g/L,Ca CO32 g/L。培养条件为温度32℃,转速180 r/min,装液量100 m L/500 m L,接种量4%,初始pH为7.0,发酵时间45 h。优化后的芽胞数量达到8.1×109cfu/m L,与优化前的芽胞数量(1.6×109cfu/m L)相比,提高了3.7倍,优化后发酵液效价达到3 350 IU/m L,提高了109%,高于同类菌株的2 000 IU/m L。 展开更多
关键词 生物防治 短短芽胞杆菌 发酵优化 响应面法
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Identification of A Strain HN-1 against Banana Wilt Disease and Determination of Its Antagonism
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作者 Shen Linbo Xiong Guoru +3 位作者 Dong Lisha Kong Ran Guo Ting Zhang Shuzhen 《Plant Diseases and Pests》 CAS 2013年第4期12-16,共5页
[Objective] The paper was to identify strain HN-1 against banana wilt disease and to determine its antagonism. [Method] The strain HN-1 was ob- tained from the soil in fields heavily infected by Fusarium oxysporum f. ... [Objective] The paper was to identify strain HN-1 against banana wilt disease and to determine its antagonism. [Method] The strain HN-1 was ob- tained from the soil in fields heavily infected by Fusarium oxysporum f. sp. cubense (FOC). Antagonism of the strain against F. oxysporum was tested via dual-cul- ture and inhibition test on spore germination. [Result] HN-1 effectively inhibited mycelial growth and spore germination of F. oxysporum, Strain HN-1 was identi- fied as BrevibaciUus brevis according to its characteristics in morphology, physiology and biochemistry and its 16S rDNA sequence. The strain showed high inhibition effect on 15 species of fungal pathogens in the dual-culture trials with fungal pathogens. [ Conclusion] The study provides theoretical basis for application of strain HN-1 in agricultural fields. 展开更多
关键词 Banana wilt disease brevibaciuus brevis HN-1 ANTAGONISM
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