Signaling within the tumor microenvironment has a critical role in cancer initiation and progression. Adipocytes, one of the major components of the breast microenvironment,have been shown to provide pro-tumorigenic s...Signaling within the tumor microenvironment has a critical role in cancer initiation and progression. Adipocytes, one of the major components of the breast microenvironment,have been shown to provide pro-tumorigenic signals that promote cancer cell proliferation and invasiveness in vitro and tumorigenicity in vivo. Adipocyte secreted factors such as leptin and interleukin-6(IL-6) have a paracrine effect on breast cancer cells. In adipocyte-adjacent breast cancer cells, the leptin and IL-6 signaling pathways activate janus kinase 2/signal transducer and activatorof transcription 5, promoting the epithelial-mesenchymal transition, and upregulating stemness regulators such as Notch, Wnt and the Sex determining region Y-box 2/octamer binding transcription factor 4/Nanog signaling axis. In this review we will summarize the major signaling pathways that regulate cancer stem cells in breast cancer and describe the effects that adipocyte secreted IL-6 and leptin have on breast cancer stem cell signaling. Finally we will introduce a new potential treatment paradigm of inhibiting the adipocyte-breast cancer cell signaling via targeting the IL-6 or leptin pathways.展开更多
目的:探究复方半边莲口服液对乳腺癌荷瘤小鼠的肿瘤抑制和改善肿瘤免疫的作用。方法:将24只BALB/C雌鼠原位接种4T1乳腺癌细胞,待瘤体体积超过40 mm 3时,随机分成对照组、复方半边莲口服液低、中、高剂量组,连续给药。每天测量荷瘤小鼠...目的:探究复方半边莲口服液对乳腺癌荷瘤小鼠的肿瘤抑制和改善肿瘤免疫的作用。方法:将24只BALB/C雌鼠原位接种4T1乳腺癌细胞,待瘤体体积超过40 mm 3时,随机分成对照组、复方半边莲口服液低、中、高剂量组,连续给药。每天测量荷瘤小鼠肿瘤大小,观察复方半边莲口服液对小鼠瘤体体积的影响;连续给药30 d后,处死荷瘤小鼠,通过测量不同组处理后荷瘤小鼠肿瘤重量,计算复方半边莲口服液各剂量组的抑瘤率;将肿瘤组织剪碎,经胶原酶处理成单细胞,并分离其中的淋巴细胞,使用流式分析仪检测肿瘤微环境中淋巴细胞的比例。结果:与对照组相比,复方半边莲口服液中、高剂量组于给药第10天、20天瘤体体积均降低(P<0.05),给药30 d,低、中、高剂量组瘤体体积均明显降低(P<0.01);低、中、高剂量组肿瘤重量明显降低(P<0.01),抑瘤率分别为17.95%、28.77%、49.43%;低、中、高剂量组CD3+T细胞、CD4+T细胞比例明显升高(P<0.01),中、高剂量组CD8+T细胞比例明显升高(P<0.01),而低、中、高剂量组调节性T细胞比例明显降低(P<0.01)。结论:复方半边莲口服液能够抑制乳腺癌荷瘤小鼠肿瘤的生长,增强荷瘤小鼠抗肿瘤免疫功能,值得临床推广应用。展开更多
目的基于知识图谱探析乳腺癌微环境的研究现状,并预测未来研究热点。方法检索中国知网及Web of Science Core Collection数据库近20年乳腺癌微环境相关文献,并运用CiteSpace、VOSviewer等软件进行文献计量学分析。结果共检索到中文文献...目的基于知识图谱探析乳腺癌微环境的研究现状,并预测未来研究热点。方法检索中国知网及Web of Science Core Collection数据库近20年乳腺癌微环境相关文献,并运用CiteSpace、VOSviewer等软件进行文献计量学分析。结果共检索到中文文献825篇,英文文献16221篇。可视化分析显示科研人员的研究重点逐渐从细胞研究转向分子方向及药物创新层面,肿瘤干细胞、PD-1、PD-L1、免疫检查点抑制剂、纳米颗粒等是目前乳腺癌微环境研究的热点。美国乳腺癌微环境的相关研究最多,其次为中国、意大利。结论目前的研究热点主要集中在肿瘤细胞干性、免疫治疗、纳米递送等领域。随着对该领域研究的不断深入,通过靶向乳腺癌微环境预防肿瘤发展及转移,改善肿瘤预后成为新的研究趋势。展开更多
文摘Signaling within the tumor microenvironment has a critical role in cancer initiation and progression. Adipocytes, one of the major components of the breast microenvironment,have been shown to provide pro-tumorigenic signals that promote cancer cell proliferation and invasiveness in vitro and tumorigenicity in vivo. Adipocyte secreted factors such as leptin and interleukin-6(IL-6) have a paracrine effect on breast cancer cells. In adipocyte-adjacent breast cancer cells, the leptin and IL-6 signaling pathways activate janus kinase 2/signal transducer and activatorof transcription 5, promoting the epithelial-mesenchymal transition, and upregulating stemness regulators such as Notch, Wnt and the Sex determining region Y-box 2/octamer binding transcription factor 4/Nanog signaling axis. In this review we will summarize the major signaling pathways that regulate cancer stem cells in breast cancer and describe the effects that adipocyte secreted IL-6 and leptin have on breast cancer stem cell signaling. Finally we will introduce a new potential treatment paradigm of inhibiting the adipocyte-breast cancer cell signaling via targeting the IL-6 or leptin pathways.
文摘目的:探究复方半边莲口服液对乳腺癌荷瘤小鼠的肿瘤抑制和改善肿瘤免疫的作用。方法:将24只BALB/C雌鼠原位接种4T1乳腺癌细胞,待瘤体体积超过40 mm 3时,随机分成对照组、复方半边莲口服液低、中、高剂量组,连续给药。每天测量荷瘤小鼠肿瘤大小,观察复方半边莲口服液对小鼠瘤体体积的影响;连续给药30 d后,处死荷瘤小鼠,通过测量不同组处理后荷瘤小鼠肿瘤重量,计算复方半边莲口服液各剂量组的抑瘤率;将肿瘤组织剪碎,经胶原酶处理成单细胞,并分离其中的淋巴细胞,使用流式分析仪检测肿瘤微环境中淋巴细胞的比例。结果:与对照组相比,复方半边莲口服液中、高剂量组于给药第10天、20天瘤体体积均降低(P<0.05),给药30 d,低、中、高剂量组瘤体体积均明显降低(P<0.01);低、中、高剂量组肿瘤重量明显降低(P<0.01),抑瘤率分别为17.95%、28.77%、49.43%;低、中、高剂量组CD3+T细胞、CD4+T细胞比例明显升高(P<0.01),中、高剂量组CD8+T细胞比例明显升高(P<0.01),而低、中、高剂量组调节性T细胞比例明显降低(P<0.01)。结论:复方半边莲口服液能够抑制乳腺癌荷瘤小鼠肿瘤的生长,增强荷瘤小鼠抗肿瘤免疫功能,值得临床推广应用。
文摘目的基于知识图谱探析乳腺癌微环境的研究现状,并预测未来研究热点。方法检索中国知网及Web of Science Core Collection数据库近20年乳腺癌微环境相关文献,并运用CiteSpace、VOSviewer等软件进行文献计量学分析。结果共检索到中文文献825篇,英文文献16221篇。可视化分析显示科研人员的研究重点逐渐从细胞研究转向分子方向及药物创新层面,肿瘤干细胞、PD-1、PD-L1、免疫检查点抑制剂、纳米颗粒等是目前乳腺癌微环境研究的热点。美国乳腺癌微环境的相关研究最多,其次为中国、意大利。结论目前的研究热点主要集中在肿瘤细胞干性、免疫治疗、纳米递送等领域。随着对该领域研究的不断深入,通过靶向乳腺癌微环境预防肿瘤发展及转移,改善肿瘤预后成为新的研究趋势。
