目的提取莼菜多糖并同时测定多糖及其胶囊制剂中的分子量和含量。方法以水提法提取莼菜多糖,经蛋白酶法和超滤等分离纯化技术,得到纯化多糖,采用HPLC-SEC测定分子量和含量。色谱柱为TSK gel G5000PWXL、G4000PWXL和G3000PWXL串联(30℃),...目的提取莼菜多糖并同时测定多糖及其胶囊制剂中的分子量和含量。方法以水提法提取莼菜多糖,经蛋白酶法和超滤等分离纯化技术,得到纯化多糖,采用HPLC-SEC测定分子量和含量。色谱柱为TSK gel G5000PWXL、G4000PWXL和G3000PWXL串联(30℃),RID(35℃),流动相为纯水,流速0.4 m L·min^(-1)。结果 45.8~1740.0 k Da多糖分子量与洗脱体积具良好的线性关系(r=0.9962),测得莼菜多糖Mw=1669.2 k Da,分散度D=1.0026;0.3751~3.751 mg·m L^(-1)多糖与峰面积呈良好的线性关系(r=0.9995),平均回收率为103.09%,95%置信度的置信区间[103.32,106.08]。结论所用方法简便、准确,且能排除辅料干扰,适用于快速同时测定多糖及其制剂的含量和分子量。展开更多
Brasenia schreberi (water shield), a perennial freshwater aquatic plant of the family Nymphaeaceae, is rich in polysaccharides with positive biological activity and potential application value. As an indicator plant o...Brasenia schreberi (water shield), a perennial freshwater aquatic plant of the family Nymphaeaceae, is rich in polysaccharides with positive biological activity and potential application value. As an indicator plant of the environment in wetlands, B. schreberi original and wild water environment faced increasing deterioration and has been listed as a critically endangered species in several countries of East Asia. Seeking an alternative method to produce B. schreberi mucilage polysaccharide is of top priority. The morphological structure of the stem and leaf of B. schreberi was studied. Well-developed glandular cells and aerenchyma tissue were observed in the stem and leaves of water shield, which were related to its aquatic habitat and polysaccharide secretion and then three endophytic bacteria were successfully isolated and purified from its stem and leaf and identified as Micromonospora sp. YG-1, Xylariaceae sp. strain UT-X and Psychrobacter pulmonis strain T-15. Symbiotic bacteria from B. schreberi produced polysaccharides with similar spectral peaks and chemical functional groups to those of B. chinensis. Further, analysis of hydroxyl free radicals showed that symbiotic bacteria in water shield had significant antioxidant activity against hydroxyl free radicals. This study provides a theoretical and practical basis for further study using symbiotic bacteria to produce polysaccharides to overcome the environmental limitations of the growth and development of B. schreberi.展开更多
文摘目的提取莼菜多糖并同时测定多糖及其胶囊制剂中的分子量和含量。方法以水提法提取莼菜多糖,经蛋白酶法和超滤等分离纯化技术,得到纯化多糖,采用HPLC-SEC测定分子量和含量。色谱柱为TSK gel G5000PWXL、G4000PWXL和G3000PWXL串联(30℃),RID(35℃),流动相为纯水,流速0.4 m L·min^(-1)。结果 45.8~1740.0 k Da多糖分子量与洗脱体积具良好的线性关系(r=0.9962),测得莼菜多糖Mw=1669.2 k Da,分散度D=1.0026;0.3751~3.751 mg·m L^(-1)多糖与峰面积呈良好的线性关系(r=0.9995),平均回收率为103.09%,95%置信度的置信区间[103.32,106.08]。结论所用方法简便、准确,且能排除辅料干扰,适用于快速同时测定多糖及其制剂的含量和分子量。
文摘Brasenia schreberi (water shield), a perennial freshwater aquatic plant of the family Nymphaeaceae, is rich in polysaccharides with positive biological activity and potential application value. As an indicator plant of the environment in wetlands, B. schreberi original and wild water environment faced increasing deterioration and has been listed as a critically endangered species in several countries of East Asia. Seeking an alternative method to produce B. schreberi mucilage polysaccharide is of top priority. The morphological structure of the stem and leaf of B. schreberi was studied. Well-developed glandular cells and aerenchyma tissue were observed in the stem and leaves of water shield, which were related to its aquatic habitat and polysaccharide secretion and then three endophytic bacteria were successfully isolated and purified from its stem and leaf and identified as Micromonospora sp. YG-1, Xylariaceae sp. strain UT-X and Psychrobacter pulmonis strain T-15. Symbiotic bacteria from B. schreberi produced polysaccharides with similar spectral peaks and chemical functional groups to those of B. chinensis. Further, analysis of hydroxyl free radicals showed that symbiotic bacteria in water shield had significant antioxidant activity against hydroxyl free radicals. This study provides a theoretical and practical basis for further study using symbiotic bacteria to produce polysaccharides to overcome the environmental limitations of the growth and development of B. schreberi.
