Tissue engineering is an interdisciplinary field that applies the principles of the engineering and the life sciences to the development of biological substitutes which restore, maintain, or improve tissue function. T...Tissue engineering is an interdisciplinary field that applies the principles of the engineering and the life sciences to the development of biological substitutes which restore, maintain, or improve tissue function. Three general strategies have been adopted for the creation of new tissue: the tissue inducing substances, cell biology, biomedical nature and the evaluation of safety. This article discussed the new development and tendency of the research in bone, cartilage, skin tissue engineering.展开更多
The extracellular matrix-associated bone morphogenetic proteins(BMPs) govern a plethora of biological processes. The BMPs are members of the transforming growth factor-β protein superfamily, and they actively partici...The extracellular matrix-associated bone morphogenetic proteins(BMPs) govern a plethora of biological processes. The BMPs are members of the transforming growth factor-β protein superfamily, and they actively participate to kidney development, digit and limb formation, angiogenesis, tissue fibrosis and tumor development. Since their discovery, they have attracted attention for their fascinating perspectives in the regenerative medicine and tissue engineering fields. BMPs have been employed in many preclinical and clinical studies exploring their chondrogenic or osteoinductive potential in several animal model defects and in human diseases. During years of research in particular two BMPs, BMP2 and BMP7 have gained the podium for their use in the treatment of various cartilage and bone defects. In particular they have been recently approved for employment in non-union fractures as adjunct therapies. On the other hand, thanks to their potentialities in biomedical applications, there is a growing interest in studying the biology of mesenchymal stem cell(MSC), the rules underneath their differentiation abilities, and to test their true abilities in tissue engineering. In fact, the specific differentiation of MSCs into targeted celltype lineages for transplantation is a primary goal of the regenerative medicine. This review provides an overview on the current knowledge of BMP roles and signaling in MSC biology and differentiation capacities. In particular the article focuses on the potential clinical use of BMPs and MSCs concomitantly, in cartilage and bone tissue repair.展开更多
背景:近20年,中医药治疗软骨疾病领域的研究逐年增多,而针对这一领域的研究文献缺乏计量分析研究来了解此领域的发展趋势。目的:基于知识图谱的方法,了解中国近20年发表的中医药治疗软骨疾病研究的知识结构和研究热点,并探索该领域的研...背景:近20年,中医药治疗软骨疾病领域的研究逐年增多,而针对这一领域的研究文献缺乏计量分析研究来了解此领域的发展趋势。目的:基于知识图谱的方法,了解中国近20年发表的中医药治疗软骨疾病研究的知识结构和研究热点,并探索该领域的研究前沿。方法:以2000至2019年中国知网和Web of Science数据库收录的关于中医药治疗软骨疾病的相关文献为研究对象,对年度发文量、期刊分布、基金分布和被引频次进行描述性统计分析,应用CiteSpace软件对机构和作者进行共现分析,对关键词进行共现及聚类分析,并绘制可视化知识图谱。结果与结论:①近20年,中国中医药治疗软骨疾病的研究热度总体呈上升趋势,国家级基金资助的文献逐渐增加,外文发文量也增长迅速,高质量的研究不断出现;②该领域以基础研究为主,探讨中医药治疗软骨疾病的作用机制,主要代表药物为补肾、活血和柔肝中药等,其中补肾法是治疗软骨疾病的主要疗法;③作者和机构虽然有开展一定的合作,但仍存在研究力量较分散、跨区域合作相对不足、缺乏影响力大的团队或领军人物等问题,如戴七一团队主要研究揉髌手法和逍遥散对软骨的影响,李荣亨团队主要研究复元胶囊对骨关节炎软骨影响等;③根据研究热点的变化可分为2000至2005年的早期中西医结合防治软骨疾病的探索阶段,2006至2013年的中药治疗骨关节炎的动物和细胞实验阶段,2014至2019年的中医针灸特色技术作用机制研究和中药与组织工程结合运用阶段;④而关于该领域的研究前沿,文章预测未来将在细胞凋亡、信号通路等微观领域和组织工程软骨、间充质干细胞分化等再生医学领域开展更多研究,而中医药与现代医学的合作成果将不断涌现。展开更多
文摘Tissue engineering is an interdisciplinary field that applies the principles of the engineering and the life sciences to the development of biological substitutes which restore, maintain, or improve tissue function. Three general strategies have been adopted for the creation of new tissue: the tissue inducing substances, cell biology, biomedical nature and the evaluation of safety. This article discussed the new development and tendency of the research in bone, cartilage, skin tissue engineering.
文摘The extracellular matrix-associated bone morphogenetic proteins(BMPs) govern a plethora of biological processes. The BMPs are members of the transforming growth factor-β protein superfamily, and they actively participate to kidney development, digit and limb formation, angiogenesis, tissue fibrosis and tumor development. Since their discovery, they have attracted attention for their fascinating perspectives in the regenerative medicine and tissue engineering fields. BMPs have been employed in many preclinical and clinical studies exploring their chondrogenic or osteoinductive potential in several animal model defects and in human diseases. During years of research in particular two BMPs, BMP2 and BMP7 have gained the podium for their use in the treatment of various cartilage and bone defects. In particular they have been recently approved for employment in non-union fractures as adjunct therapies. On the other hand, thanks to their potentialities in biomedical applications, there is a growing interest in studying the biology of mesenchymal stem cell(MSC), the rules underneath their differentiation abilities, and to test their true abilities in tissue engineering. In fact, the specific differentiation of MSCs into targeted celltype lineages for transplantation is a primary goal of the regenerative medicine. This review provides an overview on the current knowledge of BMP roles and signaling in MSC biology and differentiation capacities. In particular the article focuses on the potential clinical use of BMPs and MSCs concomitantly, in cartilage and bone tissue repair.
文摘背景:近20年,中医药治疗软骨疾病领域的研究逐年增多,而针对这一领域的研究文献缺乏计量分析研究来了解此领域的发展趋势。目的:基于知识图谱的方法,了解中国近20年发表的中医药治疗软骨疾病研究的知识结构和研究热点,并探索该领域的研究前沿。方法:以2000至2019年中国知网和Web of Science数据库收录的关于中医药治疗软骨疾病的相关文献为研究对象,对年度发文量、期刊分布、基金分布和被引频次进行描述性统计分析,应用CiteSpace软件对机构和作者进行共现分析,对关键词进行共现及聚类分析,并绘制可视化知识图谱。结果与结论:①近20年,中国中医药治疗软骨疾病的研究热度总体呈上升趋势,国家级基金资助的文献逐渐增加,外文发文量也增长迅速,高质量的研究不断出现;②该领域以基础研究为主,探讨中医药治疗软骨疾病的作用机制,主要代表药物为补肾、活血和柔肝中药等,其中补肾法是治疗软骨疾病的主要疗法;③作者和机构虽然有开展一定的合作,但仍存在研究力量较分散、跨区域合作相对不足、缺乏影响力大的团队或领军人物等问题,如戴七一团队主要研究揉髌手法和逍遥散对软骨的影响,李荣亨团队主要研究复元胶囊对骨关节炎软骨影响等;③根据研究热点的变化可分为2000至2005年的早期中西医结合防治软骨疾病的探索阶段,2006至2013年的中药治疗骨关节炎的动物和细胞实验阶段,2014至2019年的中医针灸特色技术作用机制研究和中药与组织工程结合运用阶段;④而关于该领域的研究前沿,文章预测未来将在细胞凋亡、信号通路等微观领域和组织工程软骨、间充质干细胞分化等再生医学领域开展更多研究,而中医药与现代医学的合作成果将不断涌现。
