Newly emerged larvae of the fleshfly, Boettcherisca peregrina were exposed to two different CdCl2 concentrations of 100μg/g and 400 mg/g diet fresh weight （DFW）. They were administered in the diets until the end of...Newly emerged larvae of the fleshfly, Boettcherisca peregrina were exposed to two different CdCl2 concentrations of 100μg/g and 400 mg/g diet fresh weight （DFW）. They were administered in the diets until the end of larval stage. Cd-exposed larvae accumulated significant amounts of Cd and this accumulation increased with the exposure dose and time. The body weights were lightened and lengths of larvae were shortened considerably after Cd exposure, especially at the higher Cd concentration. The total larval duration was also extremely affected due to Cd exposure. The average duration was prolonged significantly by 14 h at the lower Cd concentration, while it was increased by 33.7 h over controls at the higher Cd concentration. A significant decrease in contents of either soluble proteins, total lipids or caloric values in the hemolymph occurred due to Cd exposure throughout the entire tested period but after 120 h of Cd exposure. In contrast, when exposed to Cd with its higher concentration, total sugar contents in the hemolymph were increased strikingly over the whole tested time, except after 96 h of Cd exposure, while they were not apparently altered except after 24 h of Cd exposure at the lower concentration. Thus, it is suggested that Cd exposure shows significant adverse impact on the growth and development, as well as metabolism, in larvae of this fleshfly, depending on its exposure time and dose.展开更多
Effects of copper （Cu） accumulation by the flesh fly Boettcherisca peregrina （R.- D.） （Diptera： Sarcophagidae） on the ectoparasitic wasp Nasonia vitripennis （Walker） （Hymenoptera： Pteromalidae） were invest...Effects of copper （Cu） accumulation by the flesh fly Boettcherisca peregrina （R.- D.） （Diptera： Sarcophagidae） on the ectoparasitic wasp Nasonia vitripennis （Walker） （Hymenoptera： Pteromalidae） were investigated experimentally by exposing host larvae to contaminated diets with final Cu concentrations of 400μg/g and 800μg/g diet fresh weight （DFW）, respectively. Results showed that Cu can be transferred along food chains to secondary consumers （parasitoids） in small amounts, resulting in negative effects on parasitoid growth and development （body weight and developmental duration） as well as fecundity （number of offspring per female）. Copper exposure also inhibited vitellogenesis ofparasitoids from Cu-contaminated host pupae. It is suggested that the decreased fecundity and inhibition of vitellogenesis ofN. vitripennis resulted from poor host nutritional state rather than from direct effects of Cu stress.展开更多
[Objective] In order to find the pathogenic microorganisms suitable for biological control of filth flies, the pathogenic microorganism was isolated from the dead fly, Boettcherisca peregrine. [Method] The conidia and...[Objective] In order to find the pathogenic microorganisms suitable for biological control of filth flies, the pathogenic microorganism was isolated from the dead fly, Boettcherisca peregrine. [Method] The conidia and mycelia were observed by optical microscope. The pathogenic microorganism was identified on the basis of its culture characters and the optical morphologies of the conidia and mycelia, and its biological characteristics and pathogenicity were preliminarily studied. [Result] The pathogenic microorganism isolated from the dead fly was a new strain of Verticillium lecanii. The new strain of V. lecanii was numbered as KMZW-1. The colonies of V. lecanii KMZW-1 grew fastest on potato dextrose agar medium （PDA） at 29 ℃ and pH 6.0. The LC50 of its spore suspension to the adults of B. peregrine, Lucilia sericata, Musca domestic, Piophila casei and Drosophila melanogaster were 9.50×10^5, 4.58×10^7, 4.06×10^7, 4.10×10^3 and 1.05×10^7 conidia/ml, respectively. The LT50 were 6.86, 8.17, 8.16, 8.12 and 3.22 d, respectively. [Conclusion] V. lecanii KMZW-1 is an active pathogenic microorganism to control the adults of five fly species.展开更多
Three fibrinolytic proteases, which were designated as BPGFP1, BPGFP2 and BPGFP3 individually, were purified from the gut extract of larvae of Boettcherisca peregrina fed on artificial diet containing fibrin rich...Three fibrinolytic proteases, which were designated as BPGFP1, BPGFP2 and BPGFP3 individually, were purified from the gut extract of larvae of Boettcherisca peregrina fed on artificial diet containing fibrin rich pig blood coagulated block. BPGFP1 consists of two subunits with MW 32 000 and 30 000. Both BPGFP2 and BPGFP3 are monomer with MW 40 000 and 28 000, respectively. These three proteases are similar in substrate and inhibitor specificity. All of them possess high activities against fibrinolytic protease specific substrates such as fibrin, Chromzym P, Chromzym UK and S 2288. They also strongly hydrolyze trypsin specific substrates Bz Phe Val Arg NA, cBz Gly Pro Arg NA, Bz Pro Phe Arg NA and Bz Val Gly Arg NA. PMSF, STI, LBTI and SBBI can inhibit activity of these proteases. Activities of these three fibrinolytic proteases were found to be maximal at alkaline range of pH 9.0～10.0.展开更多
文摘Newly emerged larvae of the fleshfly, Boettcherisca peregrina were exposed to two different CdCl2 concentrations of 100μg/g and 400 mg/g diet fresh weight （DFW）. They were administered in the diets until the end of larval stage. Cd-exposed larvae accumulated significant amounts of Cd and this accumulation increased with the exposure dose and time. The body weights were lightened and lengths of larvae were shortened considerably after Cd exposure, especially at the higher Cd concentration. The total larval duration was also extremely affected due to Cd exposure. The average duration was prolonged significantly by 14 h at the lower Cd concentration, while it was increased by 33.7 h over controls at the higher Cd concentration. A significant decrease in contents of either soluble proteins, total lipids or caloric values in the hemolymph occurred due to Cd exposure throughout the entire tested period but after 120 h of Cd exposure. In contrast, when exposed to Cd with its higher concentration, total sugar contents in the hemolymph were increased strikingly over the whole tested time, except after 96 h of Cd exposure, while they were not apparently altered except after 24 h of Cd exposure at the lower concentration. Thus, it is suggested that Cd exposure shows significant adverse impact on the growth and development, as well as metabolism, in larvae of this fleshfly, depending on its exposure time and dose.
基金Acknowledgments This work was supported by the National Basic Research and Development Program of China （2006CB 102005）, the National Natural Science Foundation of China （Grants No. 30230070, 30270899）, the Program for New Century Excellent Talents in University of the Ministry of Education of China （NCET-05-0513）, and the Innovation Research Team Program of the Ministry of Education of China （Grant No. IRT0535）.
文摘Effects of copper （Cu） accumulation by the flesh fly Boettcherisca peregrina （R.- D.） （Diptera： Sarcophagidae） on the ectoparasitic wasp Nasonia vitripennis （Walker） （Hymenoptera： Pteromalidae） were investigated experimentally by exposing host larvae to contaminated diets with final Cu concentrations of 400μg/g and 800μg/g diet fresh weight （DFW）, respectively. Results showed that Cu can be transferred along food chains to secondary consumers （parasitoids） in small amounts, resulting in negative effects on parasitoid growth and development （body weight and developmental duration） as well as fecundity （number of offspring per female）. Copper exposure also inhibited vitellogenesis ofparasitoids from Cu-contaminated host pupae. It is suggested that the decreased fecundity and inhibition of vitellogenesis ofN. vitripennis resulted from poor host nutritional state rather than from direct effects of Cu stress.
基金Supported by Yunnan Provincial Department of Education,Fund Projects of Young Teachers(06y15SB)Yunnan Science and Technology Project(2006NG16)~~
文摘[Objective] In order to find the pathogenic microorganisms suitable for biological control of filth flies, the pathogenic microorganism was isolated from the dead fly, Boettcherisca peregrine. [Method] The conidia and mycelia were observed by optical microscope. The pathogenic microorganism was identified on the basis of its culture characters and the optical morphologies of the conidia and mycelia, and its biological characteristics and pathogenicity were preliminarily studied. [Result] The pathogenic microorganism isolated from the dead fly was a new strain of Verticillium lecanii. The new strain of V. lecanii was numbered as KMZW-1. The colonies of V. lecanii KMZW-1 grew fastest on potato dextrose agar medium （PDA） at 29 ℃ and pH 6.0. The LC50 of its spore suspension to the adults of B. peregrine, Lucilia sericata, Musca domestic, Piophila casei and Drosophila melanogaster were 9.50×10^5, 4.58×10^7, 4.06×10^7, 4.10×10^3 and 1.05×10^7 conidia/ml, respectively. The LT50 were 6.86, 8.17, 8.16, 8.12 and 3.22 d, respectively. [Conclusion] V. lecanii KMZW-1 is an active pathogenic microorganism to control the adults of five fly species.
文摘Three fibrinolytic proteases, which were designated as BPGFP1, BPGFP2 and BPGFP3 individually, were purified from the gut extract of larvae of Boettcherisca peregrina fed on artificial diet containing fibrin rich pig blood coagulated block. BPGFP1 consists of two subunits with MW 32 000 and 30 000. Both BPGFP2 and BPGFP3 are monomer with MW 40 000 and 28 000, respectively. These three proteases are similar in substrate and inhibitor specificity. All of them possess high activities against fibrinolytic protease specific substrates such as fibrin, Chromzym P, Chromzym UK and S 2288. They also strongly hydrolyze trypsin specific substrates Bz Phe Val Arg NA, cBz Gly Pro Arg NA, Bz Pro Phe Arg NA and Bz Val Gly Arg NA. PMSF, STI, LBTI and SBBI can inhibit activity of these proteases. Activities of these three fibrinolytic proteases were found to be maximal at alkaline range of pH 9.0～10.0.
