Background Blood culture contamination is a significant adverse event. The aim of this project was to evaluate the efficacy of a strict blood collection procedure in reducing the blood culture contamination rate. Meth...Background Blood culture contamination is a significant adverse event. The aim of this project was to evaluate the efficacy of a strict blood collection procedure in reducing the blood culture contamination rate. Methods A prospectively controlled study was performed in two different medical areas in Peking Union Medical College Hospital （PUMCH） for 16 months （from May 2006 to September 2007）. In test group, a strict blood collection procedure was carried out by trained nurses with the veinpuncture sites were scrupulously disinfected with 2.5% tincture of iodine plus 70% alcohol. In control group, commonly used procedure in PUMCH was performed with 0.45% chlorhexidine acetate plus 0.2% iodine. Blood culture positive results for 4 target organisms （Coagulase-negafive staphylococci, Propionibacterium acnes, physicians from infectious department to （contamination）. Corynebacterium species and Bacillus determine whether a sample was true species） were further assessed by positive （pathogen） or false positive Results Total 9321 blood culture collections were analyzed. The blood culture contamination rate in test group was significantly lower than that in control group （5/3177 （0.16%） vs. 77/6144 （1.25%）; x2=13.382, P 〈0.001）. The most common contaminant was Coagulase-negative staphylococcus （76.83%）. The average cultural time during which contaminated samples became positive was longer than that for true pathogen samples （42.0 hours vs. 13.9 hours, P=-0.041）. Conclusion Using a strict blood collection procedure can significantly reduce blood culture contamination rate.展开更多
目的:探讨脐带血细菌污染阳性率及不同设备在脐带血培养中的异同。方法:统计2015～2017年山东省脐带血造血干细胞库的血培养情况,比较培养天数对细菌检出率的影响,同时比对两种设备的细菌阳性率和使用情况。结果:2015年、2016年、2017...目的:探讨脐带血细菌污染阳性率及不同设备在脐带血培养中的异同。方法:统计2015～2017年山东省脐带血造血干细胞库的血培养情况,比较培养天数对细菌检出率的影响,同时比对两种设备的细菌阳性率和使用情况。结果:2015年、2016年、2017年的细菌阳性率分别为2.69%、2.33%、2.05%。培养5天能检测出99%以上的细菌,采集环节是脐带血污染的主要部分,约占90%。两种血培仪设备(法国梅里埃BacT/ALERT 3D 240和美国BD BACTECTMFX)的细菌阳性率基本相同。结论:脐带血细菌污染阳性率逐年降低,全自动化血培养5天符合检测要求,加强临床采血的无菌操作,可降低脐带血污染的几率。两种全自动化血培仪的细菌阳性率无显著差异。展开更多
文摘Background Blood culture contamination is a significant adverse event. The aim of this project was to evaluate the efficacy of a strict blood collection procedure in reducing the blood culture contamination rate. Methods A prospectively controlled study was performed in two different medical areas in Peking Union Medical College Hospital （PUMCH） for 16 months （from May 2006 to September 2007）. In test group, a strict blood collection procedure was carried out by trained nurses with the veinpuncture sites were scrupulously disinfected with 2.5% tincture of iodine plus 70% alcohol. In control group, commonly used procedure in PUMCH was performed with 0.45% chlorhexidine acetate plus 0.2% iodine. Blood culture positive results for 4 target organisms （Coagulase-negafive staphylococci, Propionibacterium acnes, physicians from infectious department to （contamination）. Corynebacterium species and Bacillus determine whether a sample was true species） were further assessed by positive （pathogen） or false positive Results Total 9321 blood culture collections were analyzed. The blood culture contamination rate in test group was significantly lower than that in control group （5/3177 （0.16%） vs. 77/6144 （1.25%）; x2=13.382, P 〈0.001）. The most common contaminant was Coagulase-negative staphylococcus （76.83%）. The average cultural time during which contaminated samples became positive was longer than that for true pathogen samples （42.0 hours vs. 13.9 hours, P=-0.041）. Conclusion Using a strict blood collection procedure can significantly reduce blood culture contamination rate.
文摘目的:探讨脐带血细菌污染阳性率及不同设备在脐带血培养中的异同。方法:统计2015～2017年山东省脐带血造血干细胞库的血培养情况,比较培养天数对细菌检出率的影响,同时比对两种设备的细菌阳性率和使用情况。结果:2015年、2016年、2017年的细菌阳性率分别为2.69%、2.33%、2.05%。培养5天能检测出99%以上的细菌,采集环节是脐带血污染的主要部分,约占90%。两种血培仪设备(法国梅里埃BacT/ALERT 3D 240和美国BD BACTECTMFX)的细菌阳性率基本相同。结论:脐带血细菌污染阳性率逐年降低,全自动化血培养5天符合检测要求,加强临床采血的无菌操作,可降低脐带血污染的几率。两种全自动化血培仪的细菌阳性率无显著差异。
