Glutamate, the most abundant excitatory neurotransmitter in the nervous system, can induce biophotonic activity and transmission in mouse brain slices. As a signaling molecule, aspartate is not considered to be an ind...Glutamate, the most abundant excitatory neurotransmitter in the nervous system, can induce biophotonic activity and transmission in mouse brain slices. As a signaling molecule, aspartate is not considered to be an independent neurotransmitter during the long evolution process, which may be just a co-transmitter or neuromodulator. In the view of structure and physiological similarities of aspartate and glutamate, as well as some differences between them, we attempted to investigate whether aspartate could also induce biophotonic activity in mouse brain slices and its effect characteristics. The ultraweak biophoton imaging system (UBIS) was used to carry out a real-time observation of biophoton activity induced by aspartate in mouse brain slices. It was found that the biophotonic emissions induced by aspartate at different concentrations (12.5 mM, 25 mM and 50 mM) presented concentration-dependent effects and 50 mM aspartate could obviously induce biophoton activities with the characteristic changes of initiation, maintenance, washing and reapplication, which were also different from that induced by 50 mM glutamate as reported before. Considering the species differences in excitatory neurotransmitters, these findings indicate that aspartate-induced biophotonic activity may imply the evolutionary differences in the animal brains.展开更多
Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly causes the destruction of small joints. The development of this disease is a relatively secret and repeated process, and ther...Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly causes the destruction of small joints. The development of this disease is a relatively secret and repeated process, and therefore early diagnosis and evaluation of the disease is usually difficult. In this study, an arthritis model was successfully induced by injecting complete Freund’s adjuvant (CFA) into the toes of lower limbs of Wistar rats. Seven days after injection of CFA, obvious redness and swelling appeared at the toe joints of lower limbs accompanied by more sensitivity to thermal stimulation. Using the ultraweak bio-photon imaging system (UBIS) established by us, the toe joint area of the lower limbs of rats was imaged 7 days after injection of CFA. It was found that the volar part of lower limbs of arthritis rats showed significantly higher biophoton emissions compared with the control group. The results of this study may provide a basis for further research and devel-opment of early diagnosis and assessment of lesion progression of rheumatoid arthritis.展开更多
The discovery of dark noise in retinal photoreceptors resulted in a long-lasting controversy over its origin and the underlying mechanisms.Here,we used a novel ultra-weak biophoton imaging system（UBIS） to detect bio...The discovery of dark noise in retinal photoreceptors resulted in a long-lasting controversy over its origin and the underlying mechanisms.Here,we used a novel ultra-weak biophoton imaging system（UBIS） to detect biophotonic activity（emission） under dark conditions in rat and bullfrog（Rana catesbeiana） retinas in vitro.We found a significant temperature-dependent increase in biophotonic activity that was completely blocked either by removing intracellular and extracellular Ca^（2＋）together or inhibiting phosphodiesterase 6.These findings suggest that the photon-like component of discrete dark noise may not be caused by a direct contribution of the thermal activation of rhodopsin,but rather by an indirect thermal induction of biophotonic activity,which then activates the retinal chromophore of rhodopsin.Therefore,this study suggests a possible solution regarding the thermal activation energy barrier for discrete dark noise,which has been debated for almost half a century.展开更多
Background: Although a large number of studies have confirmed that the different levels of reactive oxygen species (ROS) in cytoplasm and nucleus have effects on cell growth, proliferation, differentiation and apoptos...Background: Although a large number of studies have confirmed that the different levels of reactive oxygen species (ROS) in cytoplasm and nucleus have effects on cell growth, proliferation, differentiation and apoptosis, the exact mechanism of ROS action is unclear. An important reason is that the production and degradation time of ROS in cells is very short, and therefore it’s difficult to understand the mechanism of action based on the traditional molecular action process through the ROS diffusion and target binding. Methods: The fresh liver tissue slices were prepared and the nuclei of hepatocytes were separated from Kunming mice according to the reported method. Liver tissue slices and hepatocyte nuclei were perfused with extracellular or intracellular fluids containing different concentrations of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and real-time imaging monitoring of biophotonic emission was carried out using an ultra-weak biophoton imaging system. Results: The results showed that the continuous perfusion with different concentrations of H<sub>2</sub>O<sub>2</sub> (300, 400 and 500 μM, respectively) resulted in significant increase of biophotonic emissions, presenting a concentration-dependent effect in liver tissue slices and achieving the maximum effect at 400 μM, while the significant enhancement was found after 500 μM treatment on the hepatocyte nuclei. Conclusion: This study suggests that ROS generated in cells may achieve its physiological and pathological effects via biophotonic emissions, which provides a new quantum biological mechanism of ROS, while the detailed clarification requires further research.展开更多
In recent years, studies have demonstrated that biophoton is a medium for the transmission and processing of neural information. However, such studies were mainly carried out by using brain slices combined with biopho...In recent years, studies have demonstrated that biophoton is a medium for the transmission and processing of neural information. However, such studies were mainly carried out by using brain slices combined with biophoton imaging technology, while there are few reports on <i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;"> <i>vivo</i></span><span style="font-family:Verdana;"> brain biophoton imaging. In this study, the ultraweak biophoton imaging system (UBIS) was employed to carry out an </span><i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;"> <i>vivo</i></span><span style="font-family:Verdana;"> biophoton imaging for the whole brain of mice. It was found that the biophoton emission of whole brain in the slight</span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ly</span></span></span></span><span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> anesthetized mice was significantly higher than that of the background, suggesting that the brain of living mouse emits a certain intensity of stable biophotons. The biophoton imaging established in this study for the </span><i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;"> <i>vivo</i></span><span style="font-family:Verdana;"> mouse whole brain may provide a new technical method for further study of the relationship between the biophoton and brain functions, and give new ideas for developing diagnostic method of neuropsychiatric diseases.</span></span></span></span></span>展开更多
文摘Glutamate, the most abundant excitatory neurotransmitter in the nervous system, can induce biophotonic activity and transmission in mouse brain slices. As a signaling molecule, aspartate is not considered to be an independent neurotransmitter during the long evolution process, which may be just a co-transmitter or neuromodulator. In the view of structure and physiological similarities of aspartate and glutamate, as well as some differences between them, we attempted to investigate whether aspartate could also induce biophotonic activity in mouse brain slices and its effect characteristics. The ultraweak biophoton imaging system (UBIS) was used to carry out a real-time observation of biophoton activity induced by aspartate in mouse brain slices. It was found that the biophotonic emissions induced by aspartate at different concentrations (12.5 mM, 25 mM and 50 mM) presented concentration-dependent effects and 50 mM aspartate could obviously induce biophoton activities with the characteristic changes of initiation, maintenance, washing and reapplication, which were also different from that induced by 50 mM glutamate as reported before. Considering the species differences in excitatory neurotransmitters, these findings indicate that aspartate-induced biophotonic activity may imply the evolutionary differences in the animal brains.
文摘Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly causes the destruction of small joints. The development of this disease is a relatively secret and repeated process, and therefore early diagnosis and evaluation of the disease is usually difficult. In this study, an arthritis model was successfully induced by injecting complete Freund’s adjuvant (CFA) into the toes of lower limbs of Wistar rats. Seven days after injection of CFA, obvious redness and swelling appeared at the toe joints of lower limbs accompanied by more sensitivity to thermal stimulation. Using the ultraweak bio-photon imaging system (UBIS) established by us, the toe joint area of the lower limbs of rats was imaged 7 days after injection of CFA. It was found that the volar part of lower limbs of arthritis rats showed significantly higher biophoton emissions compared with the control group. The results of this study may provide a basis for further research and devel-opment of early diagnosis and assessment of lesion progression of rheumatoid arthritis.
基金supported by the National Natural Science Foundation of China (31070961)the Sci-Tech Support Plan of Hubei Province,China (2014BEC086)the Research Team Fund of South Central University for Nationalities,China (XTZ15014)
文摘The discovery of dark noise in retinal photoreceptors resulted in a long-lasting controversy over its origin and the underlying mechanisms.Here,we used a novel ultra-weak biophoton imaging system（UBIS） to detect biophotonic activity（emission） under dark conditions in rat and bullfrog（Rana catesbeiana） retinas in vitro.We found a significant temperature-dependent increase in biophotonic activity that was completely blocked either by removing intracellular and extracellular Ca^（2＋）together or inhibiting phosphodiesterase 6.These findings suggest that the photon-like component of discrete dark noise may not be caused by a direct contribution of the thermal activation of rhodopsin,but rather by an indirect thermal induction of biophotonic activity,which then activates the retinal chromophore of rhodopsin.Therefore,this study suggests a possible solution regarding the thermal activation energy barrier for discrete dark noise,which has been debated for almost half a century.
文摘Background: Although a large number of studies have confirmed that the different levels of reactive oxygen species (ROS) in cytoplasm and nucleus have effects on cell growth, proliferation, differentiation and apoptosis, the exact mechanism of ROS action is unclear. An important reason is that the production and degradation time of ROS in cells is very short, and therefore it’s difficult to understand the mechanism of action based on the traditional molecular action process through the ROS diffusion and target binding. Methods: The fresh liver tissue slices were prepared and the nuclei of hepatocytes were separated from Kunming mice according to the reported method. Liver tissue slices and hepatocyte nuclei were perfused with extracellular or intracellular fluids containing different concentrations of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and real-time imaging monitoring of biophotonic emission was carried out using an ultra-weak biophoton imaging system. Results: The results showed that the continuous perfusion with different concentrations of H<sub>2</sub>O<sub>2</sub> (300, 400 and 500 μM, respectively) resulted in significant increase of biophotonic emissions, presenting a concentration-dependent effect in liver tissue slices and achieving the maximum effect at 400 μM, while the significant enhancement was found after 500 μM treatment on the hepatocyte nuclei. Conclusion: This study suggests that ROS generated in cells may achieve its physiological and pathological effects via biophotonic emissions, which provides a new quantum biological mechanism of ROS, while the detailed clarification requires further research.
文摘In recent years, studies have demonstrated that biophoton is a medium for the transmission and processing of neural information. However, such studies were mainly carried out by using brain slices combined with biophoton imaging technology, while there are few reports on <i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;"> <i>vivo</i></span><span style="font-family:Verdana;"> brain biophoton imaging. In this study, the ultraweak biophoton imaging system (UBIS) was employed to carry out an </span><i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;"> <i>vivo</i></span><span style="font-family:Verdana;"> biophoton imaging for the whole brain of mice. It was found that the biophoton emission of whole brain in the slight</span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ly</span></span></span></span><span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> anesthetized mice was significantly higher than that of the background, suggesting that the brain of living mouse emits a certain intensity of stable biophotons. The biophoton imaging established in this study for the </span><i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;"> <i>vivo</i></span><span style="font-family:Verdana;"> mouse whole brain may provide a new technical method for further study of the relationship between the biophoton and brain functions, and give new ideas for developing diagnostic method of neuropsychiatric diseases.</span></span></span></span></span>
