AIM To study the anticancer effect of artesunate and its mechanism. METHODS To observe the effect of artesunate on the growth of solid tumor and the expression of PCNA, Bcl 2 and Bax genes in mice bearing H 22 solid h...AIM To study the anticancer effect of artesunate and its mechanism. METHODS To observe the effect of artesunate on the growth of solid tumor and the expression of PCNA, Bcl 2 and Bax genes in mice bearing H 22 solid hepatic carcinoma. RESULTS After administration of artesunate (ig, 300 mg·kg -1 ·d -1 ×7 d), growth of solid tumor was obviously inhibited, the tumor inhibitory rates were 49 1%, 48 7% and 46 6% in 3 experiments. The apoptosis of liver cancer cells were increased. Immunohistochemical staining showed that the number of Bcl 2 protein positive cells were decreased, but the number of Bax protein positive cells were increased. The PCNA positive cells were significantly lower than those in the control group. CONCLUSION Artesunate showed obvious anticancer activity on H 22 hepatic carcinoma bearing mice and undergo apoptosis of liver cancer cells. The mechanism of anticancer effect of artesunate may be related to down regulation of the expression of PCNA and Bcl 2 genes and up regulation of the expression of Bax gene.展开更多
目的研究羧甲基茯苓多糖对p388小鼠白血病的抗癌效果并探讨其治疗的分子机制。方法建立p388白血病动物模型、随机分组并给予治疗。采用流式细胞术、m R N A原位杂交和S蛳P法免疫细胞化学技术对小鼠外周血淋巴细胞的凋亡和bcl蛳2基因的m ...目的研究羧甲基茯苓多糖对p388小鼠白血病的抗癌效果并探讨其治疗的分子机制。方法建立p388白血病动物模型、随机分组并给予治疗。采用流式细胞术、m R N A原位杂交和S蛳P法免疫细胞化学技术对小鼠外周血淋巴细胞的凋亡和bcl蛳2基因的m R N A和bcl蛳2蛋白进行检测。结果羧甲基茯苓多糖组(C M P)能使荷瘤小鼠生命延长35.88%,与化疗药物环磷酰胺(C TX)合用后可使小鼠的生存期延长70.05%。C M P组的生存期与模型组比较有统计学意义(P<0.05)。C M P可通过下调bcl蛳2基因诱导癌细胞凋亡,C M P与C TX合用后可显著下调bcl蛳2基因m R N A和蛋白的表达来诱导癌细胞凋亡(与C TX比较,P<0.05)。结论C M P有很好的抗白血病作用;C M P与C TX合用后可产生协同抗癌作用。展开更多
文摘AIM To study the anticancer effect of artesunate and its mechanism. METHODS To observe the effect of artesunate on the growth of solid tumor and the expression of PCNA, Bcl 2 and Bax genes in mice bearing H 22 solid hepatic carcinoma. RESULTS After administration of artesunate (ig, 300 mg·kg -1 ·d -1 ×7 d), growth of solid tumor was obviously inhibited, the tumor inhibitory rates were 49 1%, 48 7% and 46 6% in 3 experiments. The apoptosis of liver cancer cells were increased. Immunohistochemical staining showed that the number of Bcl 2 protein positive cells were decreased, but the number of Bax protein positive cells were increased. The PCNA positive cells were significantly lower than those in the control group. CONCLUSION Artesunate showed obvious anticancer activity on H 22 hepatic carcinoma bearing mice and undergo apoptosis of liver cancer cells. The mechanism of anticancer effect of artesunate may be related to down regulation of the expression of PCNA and Bcl 2 genes and up regulation of the expression of Bax gene.
文摘目的研究羧甲基茯苓多糖对p388小鼠白血病的抗癌效果并探讨其治疗的分子机制。方法建立p388白血病动物模型、随机分组并给予治疗。采用流式细胞术、m R N A原位杂交和S蛳P法免疫细胞化学技术对小鼠外周血淋巴细胞的凋亡和bcl蛳2基因的m R N A和bcl蛳2蛋白进行检测。结果羧甲基茯苓多糖组(C M P)能使荷瘤小鼠生命延长35.88%,与化疗药物环磷酰胺(C TX)合用后可使小鼠的生存期延长70.05%。C M P组的生存期与模型组比较有统计学意义(P<0.05)。C M P可通过下调bcl蛳2基因诱导癌细胞凋亡,C M P与C TX合用后可显著下调bcl蛳2基因m R N A和蛋白的表达来诱导癌细胞凋亡(与C TX比较,P<0.05)。结论C M P有很好的抗白血病作用;C M P与C TX合用后可产生协同抗癌作用。
