AIM: To explore the correlation between expression of somatostatin (SS), gastrin (GAS) and cell apoptosis regulation gene bcl-2/bax in large intestine carcinoma.METHODS: Sixty-two large intestine cancer tissue samples...AIM: To explore the correlation between expression of somatostatin (SS), gastrin (GAS) and cell apoptosis regulation gene bcl-2/bax in large intestine carcinoma.METHODS: Sixty-two large intestine cancer tissue samples were randomly and retrospectively selected from patients with large intestine carcinoma. Immunohistochemical staining for bcl-2, bax, GAS, SS was performed according to the standard streptavidin-biotin-peroxidase (S-P) method.According to the semi-quantitative integral evaluation, SS and GAS were divided into three groups as follows. Scores1-3 were defined as the low expression group, 4-8 as the intermediate expression group, 9-16 as the high expression group. Bax and bcl-2 protein expressions in different GAS and SS expression groups of large intestine carcinoma were assessed.RESULTS: The positive expression rate of bax had a prominent difference between SS and GAS high, intermediate and low expression groups (P＜0.05, x2ss = 9.246; P＜0.05,x2GAS = 6.981). The positive expression rate of bax in SS high (80.0%, 8/10) and intermediate (76.5%, 13/17)expression groups was higher than that in low expression group (40.0%, 14/35) (P＜0.05, x2high vs low = 5.242; P＜0.05,x2middle vs low = 6.097). The positive expression rate of bax in GAS high expression group (27.3%, 3/8) was lower than that in low expression group (69.4%, 25/36) (P＜0.05,x2 = 4.594). However, bax expression in GAS intermediate expression group (46.7%, 7/15) was lower than that in low expression group, but not statistically significant. The positive expression rate of bcl-2 had a prominent difference between SS and GAS high, intermediate and low expression groups (P＜0.05, x2ss = 7.178; P＜0.05, x2GAS = 13.831). The positive expression rate of bcl-2 in GAS high (90.9%, 10/11)and intermediate (86.7%, 13/15) expression groups was higher than that in low expression group (44.4%, 16/36)(P＜0.05,x2high vs low = 5.600; P＜0.05, x2 middle vs low = 7.695).However, the positive expression rate of bcl-2 in SS high (40.0%, 4/10) and interme展开更多
目的探讨黄芩茎叶总黄酮(Scutellaria baiculensis stem-leaf total flavonoid,SSTF)对大鼠心肌缺血再灌注损伤的保护作用。方法40只SD大鼠随机分为假手术组、缺血再灌注组和SSTF预处理组。SSTF组大鼠于术前1周分别灌胃不同剂量的SSTF〔...目的探讨黄芩茎叶总黄酮(Scutellaria baiculensis stem-leaf total flavonoid,SSTF)对大鼠心肌缺血再灌注损伤的保护作用。方法40只SD大鼠随机分为假手术组、缺血再灌注组和SSTF预处理组。SSTF组大鼠于术前1周分别灌胃不同剂量的SSTF〔(17.5、35、70mg/(kg.d)〕,另两组给等量生理盐水。采用结扎大鼠左冠状动脉前降支方法制备心肌缺血再灌注模型。缺血30min、再灌注2h后,观察大鼠心电图的变化;并用免疫组织化学法检测心肌组织中Bax和Bcl-2蛋白表达情况。结果SSTF抑制了缺血再灌注损伤大鼠心电图T波升高,降低了心律失常发生率。缺血再灌注组Bcl-2蛋白表达阳性细胞率明显比假手术组低(P<0.05),Bax蛋白表达阳性细胞率明显较假手术组高,而SSTF预处理组Bcl-2蛋白表达阳性细胞率明显高于缺血再灌注组(P<0.05),Bax蛋白表达阳性细胞率明显较缺血再灌注组为低(P<0.05)。结论SSTF预处理对缺血再灌注的心肌细胞具有保护作用,其机制可能与上调Bcl-2和下调Bax蛋白表达进而抑制心肌细胞凋亡有关。展开更多
Objective： To investigate the effects ofpenehyclidine hydrochloride on apoptosis of lung tissue cells and its mechanism in acute lung injury following blunt chest trauma in rats. Methods： Sprague Dawley （SD） r...Objective： To investigate the effects ofpenehyclidine hydrochloride on apoptosis of lung tissue cells and its mechanism in acute lung injury following blunt chest trauma in rats. Methods： Sprague Dawley （SD） rats （n=54） weighing （250-25） g were divided equally and randomly into three groups： normal control group （C group, n= 18）, trauma model group （T group, n= 18） and penehyclidine hydrochloride treatment group （P group, n=18）. Each group was further divided into three subgroups according to the time points of 3, 12 and 24 hours after experiment （at each time point, n=6 for each subgroup）. Rats of P group were intraperitoneally injected with penehyclidine hydrochloride for 2 mg/kg immediately after blunt chest trauma and rats in its 24 hours subgroup were once again injected with penehyclidine hy- drochloride in the same dose 12 hours after injury. Lung tissue samples were collected at every time point and cell apoptosis in lung tissues were measured by TUNEL. Apoptotic index （AI） was calculated, expressions of bax and bcl-2 were detected by immunohistochemical staining of SABC, and lung tissue sections were taken for light and electron microscopic observation. Results： As compared with C group, at every time point, AI and expressions ofbax and bcl-2 in T group were higher （P〈0.05）, and the ratio of bcl-2/bax markedly decreased （P〈0.05）, especially in the 24 hours subgroup. The ratio in T group （0.468±0.007） was lower than that in C group （1.382±0.058, t=12.5, P〈0.01）. Lung tissue injuries were significant under a light microscope, and the number of apoptotic cells increased obviously under a transmission electron microscope. As compared with T group at the same phase, AI and expression of bax decreased in P group （P〈0.05 and P〈0.01）, while the expression of bcl-2 increased significantly （P〈0.01）, and the ratio of bcl-2/bax markedly increased （P〈0.05）, especially in the 24 hours subgroup. The ratio in P group （1.012-0.07展开更多
基金Supported by National Natural Science Foundation of China, No.39270769, Natural Science Foundation of Anhui Province, No.03043704, Natural Science Foundation of Education Bureau of Anhui Province, No.2002kj307
文摘AIM: To explore the correlation between expression of somatostatin (SS), gastrin (GAS) and cell apoptosis regulation gene bcl-2/bax in large intestine carcinoma.METHODS: Sixty-two large intestine cancer tissue samples were randomly and retrospectively selected from patients with large intestine carcinoma. Immunohistochemical staining for bcl-2, bax, GAS, SS was performed according to the standard streptavidin-biotin-peroxidase (S-P) method.According to the semi-quantitative integral evaluation, SS and GAS were divided into three groups as follows. Scores1-3 were defined as the low expression group, 4-8 as the intermediate expression group, 9-16 as the high expression group. Bax and bcl-2 protein expressions in different GAS and SS expression groups of large intestine carcinoma were assessed.RESULTS: The positive expression rate of bax had a prominent difference between SS and GAS high, intermediate and low expression groups (P＜0.05, x2ss = 9.246; P＜0.05,x2GAS = 6.981). The positive expression rate of bax in SS high (80.0%, 8/10) and intermediate (76.5%, 13/17)expression groups was higher than that in low expression group (40.0%, 14/35) (P＜0.05, x2high vs low = 5.242; P＜0.05,x2middle vs low = 6.097). The positive expression rate of bax in GAS high expression group (27.3%, 3/8) was lower than that in low expression group (69.4%, 25/36) (P＜0.05,x2 = 4.594). However, bax expression in GAS intermediate expression group (46.7%, 7/15) was lower than that in low expression group, but not statistically significant. The positive expression rate of bcl-2 had a prominent difference between SS and GAS high, intermediate and low expression groups (P＜0.05, x2ss = 7.178; P＜0.05, x2GAS = 13.831). The positive expression rate of bcl-2 in GAS high (90.9%, 10/11)and intermediate (86.7%, 13/15) expression groups was higher than that in low expression group (44.4%, 16/36)(P＜0.05,x2high vs low = 5.600; P＜0.05, x2 middle vs low = 7.695).However, the positive expression rate of bcl-2 in SS high (40.0%, 4/10) and interme
文摘目的探讨黄芩茎叶总黄酮(Scutellaria baiculensis stem-leaf total flavonoid,SSTF)对大鼠心肌缺血再灌注损伤的保护作用。方法40只SD大鼠随机分为假手术组、缺血再灌注组和SSTF预处理组。SSTF组大鼠于术前1周分别灌胃不同剂量的SSTF〔(17.5、35、70mg/(kg.d)〕,另两组给等量生理盐水。采用结扎大鼠左冠状动脉前降支方法制备心肌缺血再灌注模型。缺血30min、再灌注2h后,观察大鼠心电图的变化;并用免疫组织化学法检测心肌组织中Bax和Bcl-2蛋白表达情况。结果SSTF抑制了缺血再灌注损伤大鼠心电图T波升高,降低了心律失常发生率。缺血再灌注组Bcl-2蛋白表达阳性细胞率明显比假手术组低(P<0.05),Bax蛋白表达阳性细胞率明显较假手术组高,而SSTF预处理组Bcl-2蛋白表达阳性细胞率明显高于缺血再灌注组(P<0.05),Bax蛋白表达阳性细胞率明显较缺血再灌注组为低(P<0.05)。结论SSTF预处理对缺血再灌注的心肌细胞具有保护作用,其机制可能与上调Bcl-2和下调Bax蛋白表达进而抑制心肌细胞凋亡有关。
文摘Objective： To investigate the effects ofpenehyclidine hydrochloride on apoptosis of lung tissue cells and its mechanism in acute lung injury following blunt chest trauma in rats. Methods： Sprague Dawley （SD） rats （n=54） weighing （250-25） g were divided equally and randomly into three groups： normal control group （C group, n= 18）, trauma model group （T group, n= 18） and penehyclidine hydrochloride treatment group （P group, n=18）. Each group was further divided into three subgroups according to the time points of 3, 12 and 24 hours after experiment （at each time point, n=6 for each subgroup）. Rats of P group were intraperitoneally injected with penehyclidine hydrochloride for 2 mg/kg immediately after blunt chest trauma and rats in its 24 hours subgroup were once again injected with penehyclidine hy- drochloride in the same dose 12 hours after injury. Lung tissue samples were collected at every time point and cell apoptosis in lung tissues were measured by TUNEL. Apoptotic index （AI） was calculated, expressions of bax and bcl-2 were detected by immunohistochemical staining of SABC, and lung tissue sections were taken for light and electron microscopic observation. Results： As compared with C group, at every time point, AI and expressions ofbax and bcl-2 in T group were higher （P〈0.05）, and the ratio of bcl-2/bax markedly decreased （P〈0.05）, especially in the 24 hours subgroup. The ratio in T group （0.468±0.007） was lower than that in C group （1.382±0.058, t=12.5, P〈0.01）. Lung tissue injuries were significant under a light microscope, and the number of apoptotic cells increased obviously under a transmission electron microscope. As compared with T group at the same phase, AI and expression of bax decreased in P group （P〈0.05 and P〈0.01）, while the expression of bcl-2 increased significantly （P〈0.01）, and the ratio of bcl-2/bax markedly increased （P〈0.05）, especially in the 24 hours subgroup. The ratio in P group （1.012-0.07
