Age-stage, two-sex life tables of the melon fly, Bactrocera cucurbitae （Coquil- let-t） （Diptera： Tephritidae）, reared on cucumber （Cucumis sativus L.）, sponge gourd （Luffa cylindrica Roem） and a carrot medium...Age-stage, two-sex life tables of the melon fly, Bactrocera cucurbitae （Coquil- let-t） （Diptera： Tephritidae）, reared on cucumber （Cucumis sativus L.）, sponge gourd （Luffa cylindrica Roem） and a carrot medium （mashed Daucus carota L. mixed with sucrose and yeast hydrolysate） were constructed under laboratory conditions at 25 4- loc, 65% 4- 0.5% relative humidity, and a photoperiod 12 ： 12h （L ： D）. The intrinsic rates of increase of B. cucurbitae were 0.144 6, 0.141 2 and 0.068 8 days on cucumber, sponge gourd, and carrot medium, respectively. The highest net reproduction rate was 172 offspring per fly reared on sponge gourd. The mean generation times ofB. cucurbitae ranged from 34 days reared on cucumber to 56 days reared on carrot medium. The life history raw data was analyzed using the traditional female age-specific life table and compared to results obtained using the age-stage, two-sex life table. When the age-specific female life table is applied to an age-stage-structured two-sex population, survival and fecundity curves will be improperly manipulated due to an inability to include variation in preadult development time. We discussed different interpretations of the relationship between the net reproductive rate and the intrinsic rate of increase to clarify possible misunderstanding in the literature.展开更多
记述采自中国云南瑞丽的果实蝇属(双翅目,实蝇科) 2新种,拟黑颜果实蝇Bactrocera(Zeugodacus)proprediaphora Wang,Xiao et Chen,sp.nov.和瑞丽果实蝇Bactrocera(Bactrocera)ruiliensis Wang,Xiao et Chen,sp.nov.。2新种的正模及其中的...记述采自中国云南瑞丽的果实蝇属(双翅目,实蝇科) 2新种,拟黑颜果实蝇Bactrocera(Zeugodacus)proprediaphora Wang,Xiao et Chen,sp.nov.和瑞丽果实蝇Bactrocera(Bactrocera)ruiliensis Wang,Xiao et Chen,sp.nov.。2新种的正模及其中的4个副模标本保存于中国科学院动物研究所,其余的副模标本保存于瑞丽出入境检验检疫局。展开更多
根据2000-2004年间海南省实蝇监测和野外调查的部分研究结果，确认海南寡鬃实蝇亚科Dacinae现知下列14种：桔小实蝇Bactrocera（Bactrocera）dorsalis（Hendel），辣椒果实蝇B．（B．）Latifrons（Hendel），锈红果实蝇B．（B．）rubig...根据2000-2004年间海南省实蝇监测和野外调查的部分研究结果，确认海南寡鬃实蝇亚科Dacinae现知下列14种：桔小实蝇Bactrocera（Bactrocera）dorsalis（Hendel），辣椒果实蝇B．（B．）Latifrons（Hendel），锈红果实蝇B．（B．）rubigiha（WangetZhao），五指山果实蝇B．（B．）wuzhishana Lin et Yang，sp．nov．，何氏华实蝇B．（Sinodacus）hochii（Zia），短条果实蝇B．（Zeugodacus）abbreviata（Hardy），普通果实蝇B．（z．）caudata（Fabricius），两带果实蝇B．（Z．）cilifera（Hendel），瓜实蝇B．（Z．）cucurbitae（Coquillett），黑颜果实蝇B．（Z．）diaphora（Hendel），宽带果实蝇B．（Z．）scutellata（Hendel），南亚果实蝇B．（Z．）tau（Walker），海口棍腹实蝇Dacus（Callantra）haikouensis Wang et Chen，海南寡鬃实蝇Dacus（Dacus）hinanus Wang et Zhao。五指山果实蝇B．（B．）wuzhishana Lin et Yang，sp．nov．区别于果实蝇属内其他种的鉴别特征如下：1）颜具2个黑色大斑点；2）中胸盾片的缝后黄色侧条短而窄，后端约与缝后翅上鬃处于同一水平；3）翅前缘带较窄，末端稍加宽；4）足的股节除中股节基部的1／2和后股节基部的3／5呈黄色外，余全黑色；腹部完全黑色。除附新种特征图外，还提供海南寡鬃实蝇亚科的种类修订名录和分种检索表。展开更多
Population dynamics of Bactrocera dorsalis Hendel （Diptera： Tephritidae） were studied through pheromone trapping over 4 years （1997, 1999, 2000, 2003） in the Kunming region, a high plateau area in southwestern Ch...Population dynamics of Bactrocera dorsalis Hendel （Diptera： Tephritidae） were studied through pheromone trapping over 4 years （1997, 1999, 2000, 2003） in the Kunming region, a high plateau area in southwestern China. B. dorsalis immigrates from southern Yunnan to Kunming each year, and occurs during early May through November. Annual trap captures recorded an increase in the B. dorsalis populations from May to July, when they peaked in abundance, and a decline until November. No flies were detected from November to April. The fruit flies had two generations. There was considerable overlapping due to the continuous arrival of immigrating flies during the summer months. Annual capture rates were significantly related to numbers of flies caught in July when peak captures were recorded; whereas the peak captures, in turn, positively depended on numbers of flies recorded in May, the first month of fly appearance in the current year. It suggested that the annual population abundance was mainly dependent on the size of the initial emigrating population. A daily average temperature of 18℃ was probably the threshold temperature required for the flies to undertake long-range dispersal, which partially explained the start of the fly in May each year on this high plateau. Under field conditions, the fruit flies can withstand 13℃ as a daily average temperature. No flies were recorded in any of the study years at a daily temperature colder than 10 ℃.展开更多
Bactrocera bryoniae and Bactrocera neohumeralis are highly destructive and major biosecurity/quarantine pests of fruit and vegetable in the tropical and subtropical regions in the South Pacific and Australia.Although ...Bactrocera bryoniae and Bactrocera neohumeralis are highly destructive and major biosecurity/quarantine pests of fruit and vegetable in the tropical and subtropical regions in the South Pacific and Australia.Although these pests have not established in China,precautions must be taken due to their highly destructive nature.Thus,we predicted the potential geographic distribution of B.bryoniae and B.neohumeralis across the world and in particular China by ecological niche modeling of the Maximum Entropy(Max Ent)model with the occurrence records of these two species.Bactrocera bryoniae and B.neohumeralis exhibit similar potential geographic distribution ranges across the world and in China,and each species was predicted to be able to distribute to over 20%of the globe.Globally,the potential geographic distribution ranges for these two fruit fly species included southern Asia,the central and the southeast coast of Africa,southern North America,northern and central South America,and Australia.While within China,most of the southern Yangtze River area was found suitable for these species.Notably,southern China was considered to have the highest risk of B.bryoniae and B.neohumeralis invasions.Our study identifies the regions at high risk for potential establishment of B.bryoniae and B.neohumeralis in the world and in particular China,and informs the development of inspection and biosecurity/quarantine measures to prevent and control their invasions.展开更多
The fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) is one of the most important pests in all mango-producing areas, particularly in West Africa. In Senegal, O. americanum leaves have been used for several years...The fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) is one of the most important pests in all mango-producing areas, particularly in West Africa. In Senegal, O. americanum leaves have been used for several years to control this fly. However, to our knowledge, no chemical studies have been carried out. Thus, the aim of this study is to determine the chemical composition of the essential oil of O. americanum leaves collected in Senegal and Gambia. The essential oil obtained by hydrodistillation of these leaves is analyzed by GC/FID and GC/MS. Yields of essential oils from O. americanum leaves are 3.84% and 2.13%, respectively. Analysis of these essential oils by GC/FID and GC/MS allowed the identification of 23 compounds representing almost 100% of the total compositions. These essential oils are mainly dominated by methyleugenol (72.0% and 75.8%, respectively). Other components in significant percent are trans-β-caryophyllene (13.9% and 13.0%, respectively), germacrene D (4.1% and 3.7%, respectively), β-elemene (3.3% and 0.9%, respectively). Due to the high methyleugenol content, this study explains the attractive potential of O. americanum towards B. dorsalis. In perspective, we plan to evaluate the attractive effect of the essential oil and leaf powder of O. americanum against B. dorsalis, a real pest of mango orchards in Senegal.展开更多
The melon fruit fly, Bactrocera cucurbitae （Coquillett） （Diptera： Tephritidae）, has been the subject of worldwide quarantine and management efforts due to its widespread agricultural impact and potential for rapi...The melon fruit fly, Bactrocera cucurbitae （Coquillett） （Diptera： Tephritidae）, has been the subject of worldwide quarantine and management efforts due to its widespread agricultural impact and potential for rapid range expansion. From its presumed native distribution in India, this species has spread throughout the hot-humid regions of the world. We provide information that reveals population structure, invasion history and population connectivity from 23 locations covering nine countries based on DNA sequences of the mitochondrial cytochrome oxidase I （COI） gene. Forty-two polymorphic sites were described among 38 haplotypes. The most common haplotype, H1, was observed in 73% of the samples distributed among all populations. Highest genetic diversity was seen within populations, and no isolation-by-distance was detected. The western regions （Nepal, Bangladesh, Thailand, Burma and China-west） showed higher haplotype diversity than eastern regions （China-east）. China-Yunnan showed highest levels of genetic diversity in China. Haplotype diversity decreased with longitude from west to east. Together, these analyses suggest that B. cueurbitae has expanded from west to east within a limited geographic scale and recently invaded China through Yunnan Province.展开更多
This study evaluated the effectiveness of four Integrated Pest Management (IPM) approaches in the control of Bactrocera dorsalis (Hendel) [Diptera: Tephritidae] and Ceratitis cosyra (Walker) [Diptera: Tephritidae] dur...This study evaluated the effectiveness of four Integrated Pest Management (IPM) approaches in the control of Bactrocera dorsalis (Hendel) [Diptera: Tephritidae] and Ceratitis cosyra (Walker) [Diptera: Tephritidae] during two consecutive mango fruiting seasons (2018 and 2019) in the south-Sudanian zone of Burkina Faso. These approaches, including sanitation + M3 bait station (SM), sanitation + protein GF-120 bait (SG), sanitation + Timaye + M3 bait station (STM) and sanitation + Timaye + GF-120 bait (STG), were implemented in 12 mango orchards in three provinces of the country. In each province, one mango orchard was used as control. Flies per trap per week (FTW) and damage indices were assessed in treated orchards compared to the control orchards. The efficacy rate of each IPM approach in protecting mango against fruit fly attacks was also determined. The STG approach was the most effective in reducing both B. dorsalis and C. cosyra FTW with the best efficacy rate. Further research should emphasize indigenous and affordable attract-and-kill tools for resource poor farmers.展开更多
The detection in August 2015 of the Oriental Fruit Fly (Bactrocera dorsalis Hendel, Diptera: Tephritidae) in the Redland area in Miami-Dade County, Florida triggered a quarantine that restricted the movement of fruit ...The detection in August 2015 of the Oriental Fruit Fly (Bactrocera dorsalis Hendel, Diptera: Tephritidae) in the Redland area in Miami-Dade County, Florida triggered a quarantine that restricted the movement of fruit fly host material in an approximately 99-square mile (256-square kilometer) area. The quarantine affected 4000 acres of fruit bearing commercial avocado groves. Approved post-harvest treatments for B. dorsalis and avocado included in the USDA Treatment Manual were acceptable for immediate certification and movement of fresh avocados from the quarantine area. However, it was unknown if Florida avocados would meet quality standards (US combination grade) after the treatments. Three post-harvest treatments that combine methyl bromide fumigation and cold storage periods were tested on six avocado varieties from Florida. The treatments differed in the durations of the fumigation and cold treatment periods. A seven day transit period at 8.3°C (47°F) was added to account for the time when the fruit leaves the packing house until it is sold by retailers. None of the six varieties had met the US combination grade after the treatments and transit period. Treated fruit exhibited both internal (pulp) and external (skin) damage. Damage was attributed to the fumigation component of the treatment, but the six varieties tolerated the cold portion of the treatment. Damage by fumigation ranged from 26% - 100%. In general, the longer the fumigation period the worse the effect. The need for alternative post-harvest treatments for Florida avocados is discussed.展开更多
The Janus kinase/signal transducers and activators of transcription(JAK/STAT)signaling pathway play a pivotal role in innate immunity.Among invertebrates,Domeless receptors serve as the key upstream regulators of this...The Janus kinase/signal transducers and activators of transcription(JAK/STAT)signaling pathway play a pivotal role in innate immunity.Among invertebrates,Domeless receptors serve as the key upstream regulators of this pathway.In our study on Bactrocera dorsalis,we identified three cytokine receptors:BdDomeless1,BdDomeless2,and BdDomeless3.Each receptor encompasses five fibronectin-type-III-like(FN III)extracellular domains and a transmembrane domain.Furthermore,these receptors exhibit the increased responsiveness to diverse pathogenic challenges.Notably,only BdDomeless3 is upregulated during symbiont-like viral infections.Moreover,silencing BdDomeless3 enhanced the infectivity of Bactrocera dorsalis cripavirus(BdCV)and B.dorsalis picorna-like virus(BdPLV),underscoring BdDomeless3’s crucial role in antiviral defense of B.dorsalis.Following the suppression of Domeless3 expression,six antimicrobial peptide genes displayed decreased expression,potentially correlating with the rise in viral infectivity.To our knowledge,this is the first study identifying cytokine receptors associated with the JAK/STAT pathway in tephritid flies,shedding light on the immune mechanisms of B.dorsalis.展开更多
文摘Age-stage, two-sex life tables of the melon fly, Bactrocera cucurbitae （Coquil- let-t） （Diptera： Tephritidae）, reared on cucumber （Cucumis sativus L.）, sponge gourd （Luffa cylindrica Roem） and a carrot medium （mashed Daucus carota L. mixed with sucrose and yeast hydrolysate） were constructed under laboratory conditions at 25 4- loc, 65% 4- 0.5% relative humidity, and a photoperiod 12 ： 12h （L ： D）. The intrinsic rates of increase of B. cucurbitae were 0.144 6, 0.141 2 and 0.068 8 days on cucumber, sponge gourd, and carrot medium, respectively. The highest net reproduction rate was 172 offspring per fly reared on sponge gourd. The mean generation times ofB. cucurbitae ranged from 34 days reared on cucumber to 56 days reared on carrot medium. The life history raw data was analyzed using the traditional female age-specific life table and compared to results obtained using the age-stage, two-sex life table. When the age-specific female life table is applied to an age-stage-structured two-sex population, survival and fecundity curves will be improperly manipulated due to an inability to include variation in preadult development time. We discussed different interpretations of the relationship between the net reproductive rate and the intrinsic rate of increase to clarify possible misunderstanding in the literature.
基金the National Science Fundfor Fostering Talents in Basic Research(Special subjects in animal taxonomy,NSFC-J0630964/J0109)the National Natural Science Foundation of China(30770267)
文摘记述采自中国云南瑞丽的果实蝇属(双翅目,实蝇科) 2新种,拟黑颜果实蝇Bactrocera(Zeugodacus)proprediaphora Wang,Xiao et Chen,sp.nov.和瑞丽果实蝇Bactrocera(Bactrocera)ruiliensis Wang,Xiao et Chen,sp.nov.。2新种的正模及其中的4个副模标本保存于中国科学院动物研究所,其余的副模标本保存于瑞丽出入境检验检疫局。
文摘根据2000-2004年间海南省实蝇监测和野外调查的部分研究结果，确认海南寡鬃实蝇亚科Dacinae现知下列14种：桔小实蝇Bactrocera（Bactrocera）dorsalis（Hendel），辣椒果实蝇B．（B．）Latifrons（Hendel），锈红果实蝇B．（B．）rubigiha（WangetZhao），五指山果实蝇B．（B．）wuzhishana Lin et Yang，sp．nov．，何氏华实蝇B．（Sinodacus）hochii（Zia），短条果实蝇B．（Zeugodacus）abbreviata（Hardy），普通果实蝇B．（z．）caudata（Fabricius），两带果实蝇B．（Z．）cilifera（Hendel），瓜实蝇B．（Z．）cucurbitae（Coquillett），黑颜果实蝇B．（Z．）diaphora（Hendel），宽带果实蝇B．（Z．）scutellata（Hendel），南亚果实蝇B．（Z．）tau（Walker），海口棍腹实蝇Dacus（Callantra）haikouensis Wang et Chen，海南寡鬃实蝇Dacus（Dacus）hinanus Wang et Zhao。五指山果实蝇B．（B．）wuzhishana Lin et Yang，sp．nov．区别于果实蝇属内其他种的鉴别特征如下：1）颜具2个黑色大斑点；2）中胸盾片的缝后黄色侧条短而窄，后端约与缝后翅上鬃处于同一水平；3）翅前缘带较窄，末端稍加宽；4）足的股节除中股节基部的1／2和后股节基部的3／5呈黄色外，余全黑色；腹部完全黑色。除附新种特征图外，还提供海南寡鬃实蝇亚科的种类修订名录和分种检索表。
文摘Population dynamics of Bactrocera dorsalis Hendel （Diptera： Tephritidae） were studied through pheromone trapping over 4 years （1997, 1999, 2000, 2003） in the Kunming region, a high plateau area in southwestern China. B. dorsalis immigrates from southern Yunnan to Kunming each year, and occurs during early May through November. Annual trap captures recorded an increase in the B. dorsalis populations from May to July, when they peaked in abundance, and a decline until November. No flies were detected from November to April. The fruit flies had two generations. There was considerable overlapping due to the continuous arrival of immigrating flies during the summer months. Annual capture rates were significantly related to numbers of flies caught in July when peak captures were recorded; whereas the peak captures, in turn, positively depended on numbers of flies recorded in May, the first month of fly appearance in the current year. It suggested that the annual population abundance was mainly dependent on the size of the initial emigrating population. A daily average temperature of 18℃ was probably the threshold temperature required for the flies to undertake long-range dispersal, which partially explained the start of the fly in May each year on this high plateau. Under field conditions, the fruit flies can withstand 13℃ as a daily average temperature. No flies were recorded in any of the study years at a daily temperature colder than 10 ℃.
基金supported by the National Key R&D Program of China(2017YFC1200600 and 2016YFC1202104)the Innovation Team of Modern Agricultural Industry Generic Key Technology R&D of Guangdong Province,China(2019KJ134)+1 种基金the Open Fund of the Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,China(2016-KF-3)A student scholarship was provided by the Harry Butler Institute,Murdoch University,Australia。
文摘Bactrocera bryoniae and Bactrocera neohumeralis are highly destructive and major biosecurity/quarantine pests of fruit and vegetable in the tropical and subtropical regions in the South Pacific and Australia.Although these pests have not established in China,precautions must be taken due to their highly destructive nature.Thus,we predicted the potential geographic distribution of B.bryoniae and B.neohumeralis across the world and in particular China by ecological niche modeling of the Maximum Entropy(Max Ent)model with the occurrence records of these two species.Bactrocera bryoniae and B.neohumeralis exhibit similar potential geographic distribution ranges across the world and in China,and each species was predicted to be able to distribute to over 20%of the globe.Globally,the potential geographic distribution ranges for these two fruit fly species included southern Asia,the central and the southeast coast of Africa,southern North America,northern and central South America,and Australia.While within China,most of the southern Yangtze River area was found suitable for these species.Notably,southern China was considered to have the highest risk of B.bryoniae and B.neohumeralis invasions.Our study identifies the regions at high risk for potential establishment of B.bryoniae and B.neohumeralis in the world and in particular China,and informs the development of inspection and biosecurity/quarantine measures to prevent and control their invasions.
文摘The fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) is one of the most important pests in all mango-producing areas, particularly in West Africa. In Senegal, O. americanum leaves have been used for several years to control this fly. However, to our knowledge, no chemical studies have been carried out. Thus, the aim of this study is to determine the chemical composition of the essential oil of O. americanum leaves collected in Senegal and Gambia. The essential oil obtained by hydrodistillation of these leaves is analyzed by GC/FID and GC/MS. Yields of essential oils from O. americanum leaves are 3.84% and 2.13%, respectively. Analysis of these essential oils by GC/FID and GC/MS allowed the identification of 23 compounds representing almost 100% of the total compositions. These essential oils are mainly dominated by methyleugenol (72.0% and 75.8%, respectively). Other components in significant percent are trans-β-caryophyllene (13.9% and 13.0%, respectively), germacrene D (4.1% and 3.7%, respectively), β-elemene (3.3% and 0.9%, respectively). Due to the high methyleugenol content, this study explains the attractive potential of O. americanum towards B. dorsalis. In perspective, we plan to evaluate the attractive effect of the essential oil and leaf powder of O. americanum against B. dorsalis, a real pest of mango orchards in Senegal.
文摘The melon fruit fly, Bactrocera cucurbitae （Coquillett） （Diptera： Tephritidae）, has been the subject of worldwide quarantine and management efforts due to its widespread agricultural impact and potential for rapid range expansion. From its presumed native distribution in India, this species has spread throughout the hot-humid regions of the world. We provide information that reveals population structure, invasion history and population connectivity from 23 locations covering nine countries based on DNA sequences of the mitochondrial cytochrome oxidase I （COI） gene. Forty-two polymorphic sites were described among 38 haplotypes. The most common haplotype, H1, was observed in 73% of the samples distributed among all populations. Highest genetic diversity was seen within populations, and no isolation-by-distance was detected. The western regions （Nepal, Bangladesh, Thailand, Burma and China-west） showed higher haplotype diversity than eastern regions （China-east）. China-Yunnan showed highest levels of genetic diversity in China. Haplotype diversity decreased with longitude from west to east. Together, these analyses suggest that B. cueurbitae has expanded from west to east within a limited geographic scale and recently invaded China through Yunnan Province.
文摘This study evaluated the effectiveness of four Integrated Pest Management (IPM) approaches in the control of Bactrocera dorsalis (Hendel) [Diptera: Tephritidae] and Ceratitis cosyra (Walker) [Diptera: Tephritidae] during two consecutive mango fruiting seasons (2018 and 2019) in the south-Sudanian zone of Burkina Faso. These approaches, including sanitation + M3 bait station (SM), sanitation + protein GF-120 bait (SG), sanitation + Timaye + M3 bait station (STM) and sanitation + Timaye + GF-120 bait (STG), were implemented in 12 mango orchards in three provinces of the country. In each province, one mango orchard was used as control. Flies per trap per week (FTW) and damage indices were assessed in treated orchards compared to the control orchards. The efficacy rate of each IPM approach in protecting mango against fruit fly attacks was also determined. The STG approach was the most effective in reducing both B. dorsalis and C. cosyra FTW with the best efficacy rate. Further research should emphasize indigenous and affordable attract-and-kill tools for resource poor farmers.
文摘The detection in August 2015 of the Oriental Fruit Fly (Bactrocera dorsalis Hendel, Diptera: Tephritidae) in the Redland area in Miami-Dade County, Florida triggered a quarantine that restricted the movement of fruit fly host material in an approximately 99-square mile (256-square kilometer) area. The quarantine affected 4000 acres of fruit bearing commercial avocado groves. Approved post-harvest treatments for B. dorsalis and avocado included in the USDA Treatment Manual were acceptable for immediate certification and movement of fresh avocados from the quarantine area. However, it was unknown if Florida avocados would meet quality standards (US combination grade) after the treatments. Three post-harvest treatments that combine methyl bromide fumigation and cold storage periods were tested on six avocado varieties from Florida. The treatments differed in the durations of the fumigation and cold treatment periods. A seven day transit period at 8.3°C (47°F) was added to account for the time when the fruit leaves the packing house until it is sold by retailers. None of the six varieties had met the US combination grade after the treatments and transit period. Treated fruit exhibited both internal (pulp) and external (skin) damage. Damage was attributed to the fumigation component of the treatment, but the six varieties tolerated the cold portion of the treatment. Damage by fumigation ranged from 26% - 100%. In general, the longer the fumigation period the worse the effect. The need for alternative post-harvest treatments for Florida avocados is discussed.
基金This work was supported by the National Natural Science Foundation of China(32202278)the Chongqing Special Postdoctoral Science Foundation of Chinathe earmarked fund for China Agricultural Research System(CARS-26)。
文摘The Janus kinase/signal transducers and activators of transcription(JAK/STAT)signaling pathway play a pivotal role in innate immunity.Among invertebrates,Domeless receptors serve as the key upstream regulators of this pathway.In our study on Bactrocera dorsalis,we identified three cytokine receptors:BdDomeless1,BdDomeless2,and BdDomeless3.Each receptor encompasses five fibronectin-type-III-like(FN III)extracellular domains and a transmembrane domain.Furthermore,these receptors exhibit the increased responsiveness to diverse pathogenic challenges.Notably,only BdDomeless3 is upregulated during symbiont-like viral infections.Moreover,silencing BdDomeless3 enhanced the infectivity of Bactrocera dorsalis cripavirus(BdCV)and B.dorsalis picorna-like virus(BdPLV),underscoring BdDomeless3’s crucial role in antiviral defense of B.dorsalis.Following the suppression of Domeless3 expression,six antimicrobial peptide genes displayed decreased expression,potentially correlating with the rise in viral infectivity.To our knowledge,this is the first study identifying cytokine receptors associated with the JAK/STAT pathway in tephritid flies,shedding light on the immune mechanisms of B.dorsalis.
