AIM: To determine available information on an independent peptide transporter 1(Pep T1) and its potential relevance to treatment, this evaluation was completed.METHODS: Fully published English language literature arti...AIM: To determine available information on an independent peptide transporter 1(Pep T1) and its potential relevance to treatment, this evaluation was completed.METHODS: Fully published English language literature articles sourced through Pub Med related to protein digestion and absorption, specifically human peptide and amino acid transport, were accessed and reviewed.Papers from 1970 to the present, with particular emphasis on the past decade, were examined. In addition,abstracted information translated to English in Pub Med was also included. Finally, studies and reviews relevant to nutrient or drug uptake, particularly in human intestine were included for evaluation. This work represents a summary of all of these studies with particular reference to peptide transporter mediated assimilation of nutrients and pharmacologically active medications.RESULTS: Assimilation of dietary protein in humans involves gastric and pancreatic enzyme hydrolysis to luminal oligopeptides and free amino acids. During the ensuing intestinal phase, these hydrolytic products are transported into the epithelial cell and, eventually, the portal vein. A critical component of this process is the uptake of intact di-peptides and tri-peptides by an independent Pep T1. A number of "peptide-mimetic" pharmaceutical agents may also be transported through this carrier, important for uptake of different antibiotics, antiviral agents and angiotensin-converting enzyme inhibitors. In addition, specific peptide products of intestinal bacteria may also be transported by Pep T1, with initiation and persistence of an immune response including increased cytokine production and associated intestinal inflammatory changes. Interestingly, these inflammatory changes may also be attenuated with orallyadministered anti-inflammatory tripeptides administered as site-specific nanoparticles and taken up by this Pep T1 transport protein. CONCLUSION: Further evaluation of the role of this transporter in treatment of intestinal disorders, including inflammatory bowel 展开更多
Since 1988 the substantial development of rice transformation techniques has made it possible to introduce cloned gene into rice protoplast (by electroporation and/or PEG method) or into intact cell (by particle bomba...Since 1988 the substantial development of rice transformation techniques has made it possible to introduce cloned gene into rice protoplast (by electroporation and/or PEG method) or into intact cell (by particle bombardment). Although it was initially thought that monocot plants were not susceptible to Agrobacterium, the most widely used bacterium that mediates transformation in dicot plants,展开更多
Potent inhibitors of human peptide deformylase(HsPDF)were screened using known PMT analog inhibitors of bacterial peptide deformylase.Forty-three species of PMT analogs that are non-peptidyl bacterial PDF inhibitors l...Potent inhibitors of human peptide deformylase(HsPDF)were screened using known PMT analog inhibitors of bacterial peptide deformylase.Forty-three species of PMT analogs that are non-peptidyl bacterial PDF inhibitors like actinonin were selected using virtual screening GOLD.Ten species out of 43 that could bind to HsPDF were selected and their antitumor activities were tested.Among them,four species(PMT-172,PMT-173,PMT-199,and PMT-201)showed excellent growth inhibition of cancer cell in the MTT assay.HsPDF-PMT binding was confirmed through a1H-CPMG-T2filter NMR experiment leading to a significant change in peak intensity for PMT-172 and PMT-199.These results suggest that PMT analogs could possibly interact with HsPDF and be a novel anticancer drug candidate.展开更多
文摘AIM: To determine available information on an independent peptide transporter 1(Pep T1) and its potential relevance to treatment, this evaluation was completed.METHODS: Fully published English language literature articles sourced through Pub Med related to protein digestion and absorption, specifically human peptide and amino acid transport, were accessed and reviewed.Papers from 1970 to the present, with particular emphasis on the past decade, were examined. In addition,abstracted information translated to English in Pub Med was also included. Finally, studies and reviews relevant to nutrient or drug uptake, particularly in human intestine were included for evaluation. This work represents a summary of all of these studies with particular reference to peptide transporter mediated assimilation of nutrients and pharmacologically active medications.RESULTS: Assimilation of dietary protein in humans involves gastric and pancreatic enzyme hydrolysis to luminal oligopeptides and free amino acids. During the ensuing intestinal phase, these hydrolytic products are transported into the epithelial cell and, eventually, the portal vein. A critical component of this process is the uptake of intact di-peptides and tri-peptides by an independent Pep T1. A number of "peptide-mimetic" pharmaceutical agents may also be transported through this carrier, important for uptake of different antibiotics, antiviral agents and angiotensin-converting enzyme inhibitors. In addition, specific peptide products of intestinal bacteria may also be transported by Pep T1, with initiation and persistence of an immune response including increased cytokine production and associated intestinal inflammatory changes. Interestingly, these inflammatory changes may also be attenuated with orallyadministered anti-inflammatory tripeptides administered as site-specific nanoparticles and taken up by this Pep T1 transport protein. CONCLUSION: Further evaluation of the role of this transporter in treatment of intestinal disorders, including inflammatory bowel
基金Project supported by the Rockefeller Foundation,Academic Funds for Young Researchers of Higher Education Institutions in shanghaithe Science Committee of Shanghai.
文摘Since 1988 the substantial development of rice transformation techniques has made it possible to introduce cloned gene into rice protoplast (by electroporation and/or PEG method) or into intact cell (by particle bombardment). Although it was initially thought that monocot plants were not susceptible to Agrobacterium, the most widely used bacterium that mediates transformation in dicot plants,
基金supported by the National Research Foundation of Korea(NRF)funded by the Korean government(MEST)(2012R1A2A1A01003569&20110001207)the Korea Healthcare technology R&D Project,Ministry for Health&Welfare Affairs,Korea(A092006)supported by the Seoul Regional Small and Medium Business Administration(S1025301),in part by the 2011 BK21 project for Medicine,Dentistry,and Pharmacy
文摘Potent inhibitors of human peptide deformylase(HsPDF)were screened using known PMT analog inhibitors of bacterial peptide deformylase.Forty-three species of PMT analogs that are non-peptidyl bacterial PDF inhibitors like actinonin were selected using virtual screening GOLD.Ten species out of 43 that could bind to HsPDF were selected and their antitumor activities were tested.Among them,four species(PMT-172,PMT-173,PMT-199,and PMT-201)showed excellent growth inhibition of cancer cell in the MTT assay.HsPDF-PMT binding was confirmed through a1H-CPMG-T2filter NMR experiment leading to a significant change in peak intensity for PMT-172 and PMT-199.These results suggest that PMT analogs could possibly interact with HsPDF and be a novel anticancer drug candidate.
