Photoenzymatic catalysis has become an emerging field in organic synthetic chemistry that provides eco-friendly alternatives to traditional methods. This comprehensive review examines the developing field of photoenzy...Photoenzymatic catalysis has become an emerging field in organic synthetic chemistry that provides eco-friendly alternatives to traditional methods. This comprehensive review examines the developing field of photoenzymatic catalysis, categorized by reaction types and focusing on its application in organic synthesis. This article highlights recent advances in the use of photoenzymatic reactions in carbon-carbon cross-coupling, ketone and alkene reduction, hydroamination, and hydrosulfonylation, mostly by flavin-dependent “ene”-reductases and nitroreductases. In each case, we exemplified the substrate scope that produces products with high yield and enantioselectivity. Additionally, the emerging trends in developing new enzymatic variants and novel reaction pathways that broaden the scope and enhance yield of these reactions were discussed.展开更多
The nanomaterial-biological hybrid system(NBHS)is a rapidly growing interdisciplinary field that combines photocatalytic nanomaterials with biological systems,leveraging the superior light-harvesting capabilities of n...The nanomaterial-biological hybrid system(NBHS)is a rapidly growing interdisciplinary field that combines photocatalytic nanomaterials with biological systems,leveraging the superior light-harvesting capabilities of nanomaterials and the excellent selectivity of enzymes and microbes.This integration enables the conversion of solar energy into chemical products with high efficiency,attracting significant research interest from the fields of renewable energy and environmental science.Despite notable advances,the synergy mechanisms between abiotic nanomaterials and biotic enzymes/microbes remain unclear.This review outlines the latest progress in NBHS,encompassing material-enzyme hybrids and material-microbial hybrids,and explores design principles.Specifically,it examines the crucial role of electron transfer modes in enhancing the synergistic efficiency of nanomaterials and biological systems by analyzing various electron transfer mechanisms at the nanomaterial-biological interface.Drawing from existing literature,the review highlights the use of interfacial electron transfer mechanisms between coenzymes and cytochromes to elucidate nano/bio-material synergy.This fundamental understanding unveils opportunities to enhance biocompatible interfaces and electron transfer mechanisms,enabling non-photosensitive bacteria to harness solar energy for light-driven intracellular metabolism and CO_(2)bio-reduction into value-added chemicals.By offering a comprehensive overview of NBHS,this review also lays the groundwork for the development of more powerful systems aimed at achieving carbon neutrality.展开更多
The interest of this conference is agricultural, environmental, bioenergetics, and sanitary. In that context, domestic, agricultural and industrial environments produce organic waste, which needs to be collected, sele...The interest of this conference is agricultural, environmental, bioenergetics, and sanitary. In that context, domestic, agricultural and industrial environments produce organic waste, which needs to be collected, selected, stored and recycled properly in order to avoid environmental pollution and promote agriculture. The green Industry proposed involves the conversion of natural, non-toxic organic waste in order to efficiently produce organic fertilizers for agriculture. These types of fertilizers from biological origin are suitable because they are not toxic for human and the environment. Enzymatic reactions described in this presentation concern mainly the hydrolysis of proteins, sugars and lipids, the acidification of intermediate products from hydrolysis, the formation of acetate, and the production of methane. In other words, this review is timely as it discusses for the chemical behavior or the reactivity of different functional groups to better understand the enzymatic catalysis in the transformations of residual proteins, carbohydrates, and lipids to generate biomethane and fertilizers. In the same perspective, this review is to enrich the documentation related to organic reactions catalyzed by enzymes, which occur in the anaerobic degradation of residual organic substances, with emphasis on the structures of organic compounds and reaction mechanisms. This will allow understanding the displacement of the electrons of a reactive entity rich in electrons to another reactive entity that is poor in electrons to form new bonds in products.展开更多
The present study involves the enantioselective resolution of racemic Felodipine by using free and immobilized forms of microbial cultures as well as an enzyme (Lipase AP6). Among the microbial cultures employed in th...The present study involves the enantioselective resolution of racemic Felodipine by using free and immobilized forms of microbial cultures as well as an enzyme (Lipase AP6). Among the microbial cultures employed in the present study, Aspergillus niger, Sphingomonas paucimobilis, Cunninghamella elegans, Escherichia coli, Pseudomonas putida and Cunninghamella blakesleeana were found to possess capability of enantioselective resolution of racemic Felodipine. The enantiomeric excess (ee%) of Felodipine after reaction catalyzed by whole-cell A. niger and S. paucimobilis was found as 81.59 and 71.67%, respectively. Immobilization enhanced the enantioselectivity (enantiomeric ratio (E)) of the biocatalysts and hence this led to enhanced enantiomeric purity of the drug. The ee% values were found to be enhanced in reactions catalyzed by A. niger and S. paucimobilis cultures after immobilization as 98.27 and 93.56%, respectively. Enantiomeric ratio (E) of the reactions catalyzed by all the biocatalysts has been improved after immobilization. E value of the reaction catalyzed by immobilized A. niger was found to be excellent (E > 100) and hence the drug showed high enantiomeric purity. In lipase AP6 catalyzed study, the enantioselectivity was enhanced after immobilization with excellent E value, which led to enhanced enantiomeric purity of the drug (99.21% ee%).展开更多
The change in rheological and mechanical properties for some ionotropic cross-linked metal-alginate hydrogel complexes in particularly copper-alginate membranes in the presence of some organic solvents (benzene, tolue...The change in rheological and mechanical properties for some ionotropic cross-linked metal-alginate hydrogel complexes in particularly copper-alginate membranes in the presence of some organic solvents (benzene, toluene, xylene, carbon tetrachloride, ace-tone, chloroform, dichloroethane, isobutyl alcohol and ethyl alcohol) or buffer solutions (acetates, borates and universal buffers) have been investigated. The experimental results showed a remarkable tendency of the studied hydrogels for shrinking in polar solvents, whereas no influence was observed for the hydrogels in non-polar solvents. On the other hand, the gels were found to swell or shrink in the buffer solutions depending on the pH of the buffer used. The swelling extent for hydrogel spheres was found to decrease in the order Cu > Ba ≈ Ca > Zn > Pb-alginates in universal buffers of pH = 5.33. The factors affected this behavior have been examined and discussed.展开更多
文摘Photoenzymatic catalysis has become an emerging field in organic synthetic chemistry that provides eco-friendly alternatives to traditional methods. This comprehensive review examines the developing field of photoenzymatic catalysis, categorized by reaction types and focusing on its application in organic synthesis. This article highlights recent advances in the use of photoenzymatic reactions in carbon-carbon cross-coupling, ketone and alkene reduction, hydroamination, and hydrosulfonylation, mostly by flavin-dependent “ene”-reductases and nitroreductases. In each case, we exemplified the substrate scope that produces products with high yield and enantioselectivity. Additionally, the emerging trends in developing new enzymatic variants and novel reaction pathways that broaden the scope and enhance yield of these reactions were discussed.
基金supported by the National Key Research and Development Program of China(2021YFA0910400)National Natural Science Foundation of China(21908083)+1 种基金Natural Science Foundation of Jiangsu Province(Carbon neutralization,BK20220003)Young Talents Cultivation Program of Jiangsu University.
文摘The nanomaterial-biological hybrid system(NBHS)is a rapidly growing interdisciplinary field that combines photocatalytic nanomaterials with biological systems,leveraging the superior light-harvesting capabilities of nanomaterials and the excellent selectivity of enzymes and microbes.This integration enables the conversion of solar energy into chemical products with high efficiency,attracting significant research interest from the fields of renewable energy and environmental science.Despite notable advances,the synergy mechanisms between abiotic nanomaterials and biotic enzymes/microbes remain unclear.This review outlines the latest progress in NBHS,encompassing material-enzyme hybrids and material-microbial hybrids,and explores design principles.Specifically,it examines the crucial role of electron transfer modes in enhancing the synergistic efficiency of nanomaterials and biological systems by analyzing various electron transfer mechanisms at the nanomaterial-biological interface.Drawing from existing literature,the review highlights the use of interfacial electron transfer mechanisms between coenzymes and cytochromes to elucidate nano/bio-material synergy.This fundamental understanding unveils opportunities to enhance biocompatible interfaces and electron transfer mechanisms,enabling non-photosensitive bacteria to harness solar energy for light-driven intracellular metabolism and CO_(2)bio-reduction into value-added chemicals.By offering a comprehensive overview of NBHS,this review also lays the groundwork for the development of more powerful systems aimed at achieving carbon neutrality.
文摘The interest of this conference is agricultural, environmental, bioenergetics, and sanitary. In that context, domestic, agricultural and industrial environments produce organic waste, which needs to be collected, selected, stored and recycled properly in order to avoid environmental pollution and promote agriculture. The green Industry proposed involves the conversion of natural, non-toxic organic waste in order to efficiently produce organic fertilizers for agriculture. These types of fertilizers from biological origin are suitable because they are not toxic for human and the environment. Enzymatic reactions described in this presentation concern mainly the hydrolysis of proteins, sugars and lipids, the acidification of intermediate products from hydrolysis, the formation of acetate, and the production of methane. In other words, this review is timely as it discusses for the chemical behavior or the reactivity of different functional groups to better understand the enzymatic catalysis in the transformations of residual proteins, carbohydrates, and lipids to generate biomethane and fertilizers. In the same perspective, this review is to enrich the documentation related to organic reactions catalyzed by enzymes, which occur in the anaerobic degradation of residual organic substances, with emphasis on the structures of organic compounds and reaction mechanisms. This will allow understanding the displacement of the electrons of a reactive entity rich in electrons to another reactive entity that is poor in electrons to form new bonds in products.
文摘The present study involves the enantioselective resolution of racemic Felodipine by using free and immobilized forms of microbial cultures as well as an enzyme (Lipase AP6). Among the microbial cultures employed in the present study, Aspergillus niger, Sphingomonas paucimobilis, Cunninghamella elegans, Escherichia coli, Pseudomonas putida and Cunninghamella blakesleeana were found to possess capability of enantioselective resolution of racemic Felodipine. The enantiomeric excess (ee%) of Felodipine after reaction catalyzed by whole-cell A. niger and S. paucimobilis was found as 81.59 and 71.67%, respectively. Immobilization enhanced the enantioselectivity (enantiomeric ratio (E)) of the biocatalysts and hence this led to enhanced enantiomeric purity of the drug. The ee% values were found to be enhanced in reactions catalyzed by A. niger and S. paucimobilis cultures after immobilization as 98.27 and 93.56%, respectively. Enantiomeric ratio (E) of the reactions catalyzed by all the biocatalysts has been improved after immobilization. E value of the reaction catalyzed by immobilized A. niger was found to be excellent (E > 100) and hence the drug showed high enantiomeric purity. In lipase AP6 catalyzed study, the enantioselectivity was enhanced after immobilization with excellent E value, which led to enhanced enantiomeric purity of the drug (99.21% ee%).
文摘The change in rheological and mechanical properties for some ionotropic cross-linked metal-alginate hydrogel complexes in particularly copper-alginate membranes in the presence of some organic solvents (benzene, toluene, xylene, carbon tetrachloride, ace-tone, chloroform, dichloroethane, isobutyl alcohol and ethyl alcohol) or buffer solutions (acetates, borates and universal buffers) have been investigated. The experimental results showed a remarkable tendency of the studied hydrogels for shrinking in polar solvents, whereas no influence was observed for the hydrogels in non-polar solvents. On the other hand, the gels were found to swell or shrink in the buffer solutions depending on the pH of the buffer used. The swelling extent for hydrogel spheres was found to decrease in the order Cu > Ba ≈ Ca > Zn > Pb-alginates in universal buffers of pH = 5.33. The factors affected this behavior have been examined and discussed.
