Receptor-like kinases(RLKs) play important roles in plant immunity signaling; thus, many are hijacked by pathogen effectors to promote successful pathogenesis. Xanthomonas oryzae pv. oryzae(Xoo) is the causal agent of...Receptor-like kinases(RLKs) play important roles in plant immunity signaling; thus, many are hijacked by pathogen effectors to promote successful pathogenesis. Xanthomonas oryzae pv. oryzae(Xoo) is the causal agent of rice leaf blight disease. The strain PXO99 A has 18 non-TAL(transcription activation-like) effectors; however, their mechanisms of action and host target proteins remain largely unknown. Although the effector XopR from the Xoo strain MAFF311018 was shown to suppress PAMP-triggered immune responses in Arabidopsis, its target has not yet been identified. Here, we show that PXO99 A XopR interacts with BIK1 at the plasma membrane. BIK1 is a receptor-like cytoplasmic kinase(RLCK) belonging to the RLK family of proteins and mediates PAMP-triggered stomatal immunity. In turn, BIK1 phosphorylates XopR. Furthermore, XopR suppresses PAMP-triggered stomatal closure in transgenic Arabidopsis expressing XopR. In addition, XopR is able to associate with RLCKs other than BIK1. These results suggest that XopR likely suppresses plant immunity by targeting BIK1 and other RLCKs.展开更多
Lane-changing behaviour is one of the complex|driving behaviours.The lane-changing behaviour of drivers may exacerbate congestion,however driver behavioural characteristics are difficult to accurately acquire and quan...Lane-changing behaviour is one of the complex|driving behaviours.The lane-changing behaviour of drivers may exacerbate congestion,however driver behavioural characteristics are difficult to accurately acquire and quantify,and thus tend to be simplified or ignored in existing lane-changing models.In this paper,the Bik-means clustering algorithm is used to analyse the urban road congestion state discrimination method.Then,simulated driving tests were conducted for different traffic congestion conditions.Through the force feedback system and infrared camera,the data of driver lane-changing behaviours at different traffic congestion levels are obtained separately,and the definitions of the start and end points of a vehicle changing lanes are determined.Furthermore,statistical analysis and discussion of key feature parameters including driver lane-changing behaviour data and visual data under different levels of traffic congestion were conducted.It is found that the average lane-change intention times in each congestion state are 2 s,4 s,6 s and 7 s,while the turn-signal duration and the number of rear-view mirror observations have similar patterns of change to the data on lane-changing intention duration.Moreover,drivers’pupil diameters become smaller during the lane-changing intention phase,and then relatively enlarge during lane-changing;the range of pupil variation is roughly 3.5 mm to 4 mm.The frequency of observing the vehicle in front of the target lane increased as the level of congestion increased,and the frequency of observation in the driver’s mirrors while changing lanes approximately doubled compared to driving straight ahead,and this ratio increased as the level of congestion increased.展开更多
Summary In Arabidopsis, both the membrane-anchored receptor-like kinase （RLK） BAK1 and the receptor-like cytoplasmic kinase （RLCK） BIK1 are important mediators of transmembrane signal transduction that regulate pl...Summary In Arabidopsis, both the membrane-anchored receptor-like kinase （RLK） BAK1 and the receptor-like cytoplasmic kinase （RLCK） BIK1 are important mediators of transmembrane signal transduction that regulate plant development and immunity. However, little attention has been paid to their genetic association. This study found the bak1 bik1 double mutant of Arabidopsis displayed a severe dwarfism phenotype due to constitutive immunity and cell death in developing plants. These data suggest that BIK1 cooperates with BAK1 to regulate constitutive immunity and cell death.展开更多
基金National Natural Science Foundation of China(Grant No.60775038)Ningbo Natural Science Foundation of China(Grant No.2009A610078)K.C.Wang Magna Fund in Ningbo University
基金supported by the National Natural Science Foundation of China(31322009)the National Basic Research Program of China(2015CB910200)the State Key Laboratory of Plant Genomics of China(SKLPG2011B0301,SKLPG2011A0301)
文摘Receptor-like kinases(RLKs) play important roles in plant immunity signaling; thus, many are hijacked by pathogen effectors to promote successful pathogenesis. Xanthomonas oryzae pv. oryzae(Xoo) is the causal agent of rice leaf blight disease. The strain PXO99 A has 18 non-TAL(transcription activation-like) effectors; however, their mechanisms of action and host target proteins remain largely unknown. Although the effector XopR from the Xoo strain MAFF311018 was shown to suppress PAMP-triggered immune responses in Arabidopsis, its target has not yet been identified. Here, we show that PXO99 A XopR interacts with BIK1 at the plasma membrane. BIK1 is a receptor-like cytoplasmic kinase(RLCK) belonging to the RLK family of proteins and mediates PAMP-triggered stomatal immunity. In turn, BIK1 phosphorylates XopR. Furthermore, XopR suppresses PAMP-triggered stomatal closure in transgenic Arabidopsis expressing XopR. In addition, XopR is able to associate with RLCKs other than BIK1. These results suggest that XopR likely suppresses plant immunity by targeting BIK1 and other RLCKs.
基金supported in part by funds from Heilongjiang Provincial Key R&D Programme(Grant No.JD22A014)the Fundamental Research Funds for the Central Universities(Grant No.2572021AW35).
文摘Lane-changing behaviour is one of the complex|driving behaviours.The lane-changing behaviour of drivers may exacerbate congestion,however driver behavioural characteristics are difficult to accurately acquire and quantify,and thus tend to be simplified or ignored in existing lane-changing models.In this paper,the Bik-means clustering algorithm is used to analyse the urban road congestion state discrimination method.Then,simulated driving tests were conducted for different traffic congestion conditions.Through the force feedback system and infrared camera,the data of driver lane-changing behaviours at different traffic congestion levels are obtained separately,and the definitions of the start and end points of a vehicle changing lanes are determined.Furthermore,statistical analysis and discussion of key feature parameters including driver lane-changing behaviour data and visual data under different levels of traffic congestion were conducted.It is found that the average lane-change intention times in each congestion state are 2 s,4 s,6 s and 7 s,while the turn-signal duration and the number of rear-view mirror observations have similar patterns of change to the data on lane-changing intention duration.Moreover,drivers’pupil diameters become smaller during the lane-changing intention phase,and then relatively enlarge during lane-changing;the range of pupil variation is roughly 3.5 mm to 4 mm.The frequency of observing the vehicle in front of the target lane increased as the level of congestion increased,and the frequency of observation in the driver’s mirrors while changing lanes approximately doubled compared to driving straight ahead,and this ratio increased as the level of congestion increased.
基金supported by the National Natural Science Foundation of China(31571249 and 31425003)
文摘Summary In Arabidopsis, both the membrane-anchored receptor-like kinase （RLK） BAK1 and the receptor-like cytoplasmic kinase （RLCK） BIK1 are important mediators of transmembrane signal transduction that regulate plant development and immunity. However, little attention has been paid to their genetic association. This study found the bak1 bik1 double mutant of Arabidopsis displayed a severe dwarfism phenotype due to constitutive immunity and cell death in developing plants. These data suggest that BIK1 cooperates with BAK1 to regulate constitutive immunity and cell death.
