AIM To study the expression of Fas and Bcl 2 proteins in BEL 7404 human hepatoma cells in order to analyze the possible relationship between cell growth regulation by alpha fetoprotein(AFP) and Fas/Bcl 2 prot...AIM To study the expression of Fas and Bcl 2 proteins in BEL 7404 human hepatoma cells in order to analyze the possible relationship between cell growth regulation by alpha fetoprotein(AFP) and Fas/Bcl 2 proteins. METHODS BEL 7404 human hepatoma cells were maintained in RPMI 1640 medium supplemented with 10% new born calf serum. Cells adhered to coverslips were used to detect Fas and Bcl 2 protein expression by the avidin biotin complex (ABC) immunocytochemical assay. RESULTS Immunocytochemical study showed that essentially all the BEL 7404 human hepatoma cells could express Fas and Bcl 2 proteins, although in various amounts. No positive staining for Fas and Bcl 2 proteins was observed when cells were incubated with non relevant sera to establish the specificity. CONCLUSION Fas apoptosis signals and Bcl 2 rescue/survival signals from apoptosis are expressed in BEL 7404 human hepatoma cells. The finding strongly implys that AFP mediated cell apoptosis and growth enhancement are potentially associated with Fas and Bcl 2 proteins present in those cells.展开更多
BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified...BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified in phloem cells. One of these, StBEL5, and its Knox protein partner regulate tuber formation by targeting genes that control growth. RNA detection methods and grafting experiments demonstrated that StBEL5 transcripts move across a graft union to localize in stolon tips, the site of tuber induction. This movement of RNA originates in source leaf veins and petioles and is induced by a short-day photoperiod, regulated by the untranslated regions, and correlated with enhanced tuber production. Addition of the StBEL5 untranslated regions to another BELl.like mRNA resulted in its preferential transport to stolon tips leading to increased tuber production. Upon fusion of the untranslated regions of StBEL5 to a β-glucuronidase marker, translation in tobacco protoplasts was repressed by those constructs containing the 3＇ untranslated sequence. The untranslated regions of the StBEL5 mRNA are involved in mediating its long-distance transport and in controlling translation. The 3＇ untranslated sequence contains an abundance of conserved motifs that may serve as binding motifs for RNA-binding proteins. Because of their presence in the phloem sieve tube system, their unique untranslated region sequences and their diverse RNA accumulation patterns, the family of BEL1-like RNAs from potato represents a valuable model for studying the long-distance transport of full-length mRNAs and their role in development.展开更多
In order to characterize the forest fires in the province of Sidi Bel Abbes （North West of Algeria） and depending on a statistical data analysis of a 17-year period （1996-2012）, our works are oriented toward a stu...In order to characterize the forest fires in the province of Sidi Bel Abbes （North West of Algeria） and depending on a statistical data analysis of a 17-year period （1996-2012）, our works are oriented toward a study of the area covered by forest fires in the 53 constituent municipalities of the region. To do this, we depended on the archives of the forest fires balance sheets in forest conservation in the wilaya of Sidi Bel Abbes. The results show that the region lost an annual average 29,929 ha of its total forest area for a maximum of 11,817.5 ha recorded in 2012. On the other hand, the use of the fire severity index ＂FSI＂ by town and its cartography in which we used the map of administrative division of the study area as support, has divided the region into six classes according to the risk of fire, where the municipalities belonging to the forest of Telagh whose land is rugged represent the risk of fire is greater. Indeed, this situation category the study area among the most exposed wilaya and the most affected by this problem at the national level and require new approaches to forest management for its conservation. These are paramount in reducing the risk of fires based on appropriate management plans to be established for each forest conservation area.展开更多
Both Chinese national vocal music and bel canto have a very long history of development, but have their own different characteristics. However, the two different ways are mutually influenced and connected along with t...Both Chinese national vocal music and bel canto have a very long history of development, but have their own different characteristics. However, the two different ways are mutually influenced and connected along with the social economic development and globalization. This requires the absorption of the excellent western singing styles on the basis of developing Chinese national vocal music, so as to promote a common development. In this paper, Chinese national vocal music and bel canto are studied and analyzed according to the historical origins, different characteristics, effects, etc.展开更多
文摘AIM To study the expression of Fas and Bcl 2 proteins in BEL 7404 human hepatoma cells in order to analyze the possible relationship between cell growth regulation by alpha fetoprotein(AFP) and Fas/Bcl 2 proteins. METHODS BEL 7404 human hepatoma cells were maintained in RPMI 1640 medium supplemented with 10% new born calf serum. Cells adhered to coverslips were used to detect Fas and Bcl 2 protein expression by the avidin biotin complex (ABC) immunocytochemical assay. RESULTS Immunocytochemical study showed that essentially all the BEL 7404 human hepatoma cells could express Fas and Bcl 2 proteins, although in various amounts. No positive staining for Fas and Bcl 2 proteins was observed when cells were incubated with non relevant sera to establish the specificity. CONCLUSION Fas apoptosis signals and Bcl 2 rescue/survival signals from apoptosis are expressed in BEL 7404 human hepatoma cells. The finding strongly implys that AFP mediated cell apoptosis and growth enhancement are potentially associated with Fas and Bcl 2 proteins present in those cells.
基金supported by the United States Departmentof Agriculture NRI-CGP award no.2008-02806National Science Foundation-Plant Genome Research Program award no.0820659
文摘BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified in phloem cells. One of these, StBEL5, and its Knox protein partner regulate tuber formation by targeting genes that control growth. RNA detection methods and grafting experiments demonstrated that StBEL5 transcripts move across a graft union to localize in stolon tips, the site of tuber induction. This movement of RNA originates in source leaf veins and petioles and is induced by a short-day photoperiod, regulated by the untranslated regions, and correlated with enhanced tuber production. Addition of the StBEL5 untranslated regions to another BELl.like mRNA resulted in its preferential transport to stolon tips leading to increased tuber production. Upon fusion of the untranslated regions of StBEL5 to a β-glucuronidase marker, translation in tobacco protoplasts was repressed by those constructs containing the 3＇ untranslated sequence. The untranslated regions of the StBEL5 mRNA are involved in mediating its long-distance transport and in controlling translation. The 3＇ untranslated sequence contains an abundance of conserved motifs that may serve as binding motifs for RNA-binding proteins. Because of their presence in the phloem sieve tube system, their unique untranslated region sequences and their diverse RNA accumulation patterns, the family of BEL1-like RNAs from potato represents a valuable model for studying the long-distance transport of full-length mRNAs and their role in development.
文摘In order to characterize the forest fires in the province of Sidi Bel Abbes （North West of Algeria） and depending on a statistical data analysis of a 17-year period （1996-2012）, our works are oriented toward a study of the area covered by forest fires in the 53 constituent municipalities of the region. To do this, we depended on the archives of the forest fires balance sheets in forest conservation in the wilaya of Sidi Bel Abbes. The results show that the region lost an annual average 29,929 ha of its total forest area for a maximum of 11,817.5 ha recorded in 2012. On the other hand, the use of the fire severity index ＂FSI＂ by town and its cartography in which we used the map of administrative division of the study area as support, has divided the region into six classes according to the risk of fire, where the municipalities belonging to the forest of Telagh whose land is rugged represent the risk of fire is greater. Indeed, this situation category the study area among the most exposed wilaya and the most affected by this problem at the national level and require new approaches to forest management for its conservation. These are paramount in reducing the risk of fires based on appropriate management plans to be established for each forest conservation area.
文摘Both Chinese national vocal music and bel canto have a very long history of development, but have their own different characteristics. However, the two different ways are mutually influenced and connected along with the social economic development and globalization. This requires the absorption of the excellent western singing styles on the basis of developing Chinese national vocal music, so as to promote a common development. In this paper, Chinese national vocal music and bel canto are studied and analyzed according to the historical origins, different characteristics, effects, etc.
