对亲本披碱草(E lym us d ahuricus)和野大麦(H ord eum brev isubu la tum)及其杂种F1和BC1代体细胞染色体数和花粉母细胞(PM C)减数分裂染色体行为进行观察和分析。结果表明:亲本披碱草的染色体数为2n=42,野大麦为2n=28;正、反交杂种F...对亲本披碱草(E lym us d ahuricus)和野大麦(H ord eum brev isubu la tum)及其杂种F1和BC1代体细胞染色体数和花粉母细胞(PM C)减数分裂染色体行为进行观察和分析。结果表明:亲本披碱草的染色体数为2n=42,野大麦为2n=28;正、反交杂种F1染色体数均为35;BC1代染色体发生了数目上的差异,变异范围为28~35,并出现很多非整倍性植株;披碱草的PM CM I二价体配对频率很高,野大麦PM CM I出版少量的四价体、三价体和单价体,但以二价体占优势;正反交杂种F1减数分裂不规则,单价体的普遍存在是造成杂交不育的重要原因;BC1代的PM CM I单价体频率较F1代有所下降,二价体频率有所增高,有些株系的环状二价体占绝对优势,这为杂种育性恢复提供了细胞学依据。展开更多
In four rice genomes,85 ABC1-family genes were identified by comparative genomics,evolution,genetics,and physiology.One,OsABC1-13,was shown by knockdown and knockout experiments to affect plant height,grain size,and p...In four rice genomes,85 ABC1-family genes were identified by comparative genomics,evolution,genetics,and physiology.One,OsABC1-13,was shown by knockdown and knockout experiments to affect plant height,grain size,and photosynthetic capability.展开更多
Members of the activity of bc1 complex (ABC1) family are protein kinases that are widely found in prokaryotes and eukaryotes. Previous studies showed that several plant ABC1 genes participated in the abiotic stress re...Members of the activity of bc1 complex (ABC1) family are protein kinases that are widely found in prokaryotes and eukaryotes. Previous studies showed that several plant ABC1 genes participated in the abiotic stress response. Here, we present the systematic identification of rice and Arabidopsis ABC1 genes and the expression analysis of rice ABC1 genes. A total of 15 and 17 ABC1 genes from the rice and Arabidopsis genomes, respectively, were identified using a bioinformatics approach. Phylogenetic analyses of these proteins suggested that the divergence of this family had occurred and their main characteristics were established before the monocot-dicot split. Indeed, species-specific expansion contributed to the evolution of this family in rice and Arabidopsis after the monocot-dicot split. Intron/exon structure analysis indicated that most of the orthologous genes had similar exon sizes, but diverse intron sizes, and the rice genes contained larger introns, moreover, intron gain was an important event accompanying the recent evolution of the rice ABC1 family. Multiple sequence alignment revealed one conserved amino acid segment and four conserved amino acids in the ABC1 domain. Online subcellular localization predicted that nine rice ABC1 proteins were localized in chloroplasts. Real-time RT-PCR established that the rice ABC1 genes were primarily expressed in leaves and the expression could be modulated by a broad range of abiotic factors such as H2O2, abscisic acid, low temperature, drought, darkness and high salinity. These results reveal that the rice ABC1 gene family plays roles in the environmental stress response and specific biological processes of rice.展开更多
A simple method to obtain large red crystals of cytochrome bc1 complex from beef heart mitochondria has been developed. These crystals are very stable. Their shapes are retained for a long time in tip-sealed Pasteur p...A simple method to obtain large red crystals of cytochrome bc1 complex from beef heart mitochondria has been developed. These crystals are very stable. Their shapes are retained for a long time in tip-sealed Pasteur pipets placed in a refrigerator. The structure of crystalline cytochrome bc1 complex by micro FT-IR spectroscopy has been investigated. Based on the IR spectra of cytochrome c, the empirical assignments of the major infrared frequencies of cytochrome bc1 complex are given. Infrared frequencies and relative intensities of variable orientation and section of crystal are significantly different. These imply that infrared spectral characterization of the membrane protein crystallization is associated with the variable symmetries and orientations of the structure. Experimental results show that phospholipid exists in the crystal of cytochrome bc1 complex. The membrane protein is probably spanned on the mitochondrial membrane and buried in phospholipid bilayer in an asymmetric manner.展开更多
德国Burmester柏林之声在1977年创立于柏林,并以极快的速度崛起于德国High-End音响工业。产品包括前级、功率放大器、CD播放机、CD转盘、D/A解码器、音箱及其它附件等,其产品涵盖不同的价位。现已成为世界上最受推崇的顶级立体音响系统...德国Burmester柏林之声在1977年创立于柏林,并以极快的速度崛起于德国High-End音响工业。产品包括前级、功率放大器、CD播放机、CD转盘、D/A解码器、音箱及其它附件等,其产品涵盖不同的价位。现已成为世界上最受推崇的顶级立体音响系统制造厂家之一。也是顶级音响界极少数可从讯源、扩大机到喇叭全系列都能一手掌握,做到全产业链并坚持原产地手工设计制造的品牌。Burmester坚持“Art for the ear”(为聆听而创造的艺术品)的产品理念,让消费者享受到最纯粹的“ 德国制造”是Burmester不变的信念。也正因这份坚持,让Burmester成为世界影音发烧友心目中的逸品。展开更多
Making sense of Mark Antony＇s political and military dealings in the Eastern Mediterranean in the period from the Battle of Philippi （in 42 BC）2 until the Battle of Actium （in 31） is not a straightforward matter....Making sense of Mark Antony＇s political and military dealings in the Eastern Mediterranean in the period from the Battle of Philippi （in 42 BC）2 until the Battle of Actium （in 31） is not a straightforward matter. With Antony having been brought into disrepute under the hand of OctaviardAugustus, modern observers are constantly faced with the difficulty of establishing to what extent the literary sources on which our knowledge of these eastern affairs largely depends.展开更多
文摘对亲本披碱草(E lym us d ahuricus)和野大麦(H ord eum brev isubu la tum)及其杂种F1和BC1代体细胞染色体数和花粉母细胞(PM C)减数分裂染色体行为进行观察和分析。结果表明:亲本披碱草的染色体数为2n=42,野大麦为2n=28;正、反交杂种F1染色体数均为35;BC1代染色体发生了数目上的差异,变异范围为28~35,并出现很多非整倍性植株;披碱草的PM CM I二价体配对频率很高,野大麦PM CM I出版少量的四价体、三价体和单价体,但以二价体占优势;正反交杂种F1减数分裂不规则,单价体的普遍存在是造成杂交不育的重要原因;BC1代的PM CM I单价体频率较F1代有所下降,二价体频率有所增高,有些株系的环状二价体占绝对优势,这为杂种育性恢复提供了细胞学依据。
基金supported by the Innovation Program of the Shanghai Municipal Education Commission(2023ZKZD05)the Shanghai Oriental Talent(Rural Revitalization)Top Talent Project(T2023102).
文摘In four rice genomes,85 ABC1-family genes were identified by comparative genomics,evolution,genetics,and physiology.One,OsABC1-13,was shown by knockdown and knockout experiments to affect plant height,grain size,and photosynthetic capability.
文摘采用发酵-渗透汽化耦合(fermentation coupling with pervaporation,FCP)系统和分批补料式发酵系统培养Pseudomonas aeruginosa BC1制备生物表面活性剂鼠李糖脂,两轮发酵过程分别持续124 h和107 h。在FCP系统中,最大细胞吸光度OD600为1.06;生物表面活性剂OD600为0.61;鼠李糖脂最终产量为1.3 g/L,相比于分批补料式发酵系统提高38%;对比纯水的表面张力67.52 m N/m,发酵第29 h的发酵上清液表面张力为22.56 m N/m。同时,该菌的发酵上清液对液体石蜡、机油、柴油、正己烷、十六烷均有较好的乳化能力。经GC-MS结合SPME分析发现,FCP系统分离出的渗透蒸汽冷凝液中含有乙醇、戊醇等有机物。实验结果表明,相比分批补料式发酵系统,FCP系统能够分离发酵过程中产生的一部分挥发性代谢产物,减轻这些物质对细胞生长的抑制,使细胞浓度和鼠李糖脂产量都有明显提高。
基金supported by grants from the National Program on the Development of Basic Research of China (Grant No. 2006CB101700)the National Natural Science Foundation of China (Grant No. 30971846)the Vital Project of Natural Science in Universities of Jiangsu Province, China (Grant No. 09KJA210002)
文摘Members of the activity of bc1 complex (ABC1) family are protein kinases that are widely found in prokaryotes and eukaryotes. Previous studies showed that several plant ABC1 genes participated in the abiotic stress response. Here, we present the systematic identification of rice and Arabidopsis ABC1 genes and the expression analysis of rice ABC1 genes. A total of 15 and 17 ABC1 genes from the rice and Arabidopsis genomes, respectively, were identified using a bioinformatics approach. Phylogenetic analyses of these proteins suggested that the divergence of this family had occurred and their main characteristics were established before the monocot-dicot split. Indeed, species-specific expansion contributed to the evolution of this family in rice and Arabidopsis after the monocot-dicot split. Intron/exon structure analysis indicated that most of the orthologous genes had similar exon sizes, but diverse intron sizes, and the rice genes contained larger introns, moreover, intron gain was an important event accompanying the recent evolution of the rice ABC1 family. Multiple sequence alignment revealed one conserved amino acid segment and four conserved amino acids in the ABC1 domain. Online subcellular localization predicted that nine rice ABC1 proteins were localized in chloroplasts. Real-time RT-PCR established that the rice ABC1 genes were primarily expressed in leaves and the expression could be modulated by a broad range of abiotic factors such as H2O2, abscisic acid, low temperature, drought, darkness and high salinity. These results reveal that the rice ABC1 gene family plays roles in the environmental stress response and specific biological processes of rice.
基金Project supported by the National Natural Science Foundation of China.
文摘A simple method to obtain large red crystals of cytochrome bc1 complex from beef heart mitochondria has been developed. These crystals are very stable. Their shapes are retained for a long time in tip-sealed Pasteur pipets placed in a refrigerator. The structure of crystalline cytochrome bc1 complex by micro FT-IR spectroscopy has been investigated. Based on the IR spectra of cytochrome c, the empirical assignments of the major infrared frequencies of cytochrome bc1 complex are given. Infrared frequencies and relative intensities of variable orientation and section of crystal are significantly different. These imply that infrared spectral characterization of the membrane protein crystallization is associated with the variable symmetries and orientations of the structure. Experimental results show that phospholipid exists in the crystal of cytochrome bc1 complex. The membrane protein is probably spanned on the mitochondrial membrane and buried in phospholipid bilayer in an asymmetric manner.
文摘德国Burmester柏林之声在1977年创立于柏林,并以极快的速度崛起于德国High-End音响工业。产品包括前级、功率放大器、CD播放机、CD转盘、D/A解码器、音箱及其它附件等,其产品涵盖不同的价位。现已成为世界上最受推崇的顶级立体音响系统制造厂家之一。也是顶级音响界极少数可从讯源、扩大机到喇叭全系列都能一手掌握,做到全产业链并坚持原产地手工设计制造的品牌。Burmester坚持“Art for the ear”(为聆听而创造的艺术品)的产品理念,让消费者享受到最纯粹的“ 德国制造”是Burmester不变的信念。也正因这份坚持,让Burmester成为世界影音发烧友心目中的逸品。
文摘Making sense of Mark Antony＇s political and military dealings in the Eastern Mediterranean in the period from the Battle of Philippi （in 42 BC）2 until the Battle of Actium （in 31） is not a straightforward matter. With Antony having been brought into disrepute under the hand of OctaviardAugustus, modern observers are constantly faced with the difficulty of establishing to what extent the literary sources on which our knowledge of these eastern affairs largely depends.
