Avian influenza A（H7N9） virus is one subgroup among the larger group of H7 viruses,which normally circulate among birds.The H7N9 subtype of avian influenza viruses has not been known to infect humans until only rece...Avian influenza A（H7N9） virus is one subgroup among the larger group of H7 viruses,which normally circulate among birds.The H7N9 subtype of avian influenza viruses has not been known to infect humans until only recently.On March 31,2013,China confirmed the first three human cases of novel avian influenza A（H7N9）infection in Shanghai and Anhui,two of these patients died.1 As of February 27,2014,367 laboratory-confirmed human cases have been reported from 15 provinces/municipalities in China's Mainland,展开更多
文摘目的 探讨H7N9禽流感患者外周血T淋巴细胞亚群及相关细胞因子的变化及临床意义。方法 2013年4月至2015年4月在苏州大学附属第一医院救治的H7N9禽流感患者23例作为H7N9感染组，选取同期在该院门诊体检健康者20名作为健康对照组。采用流式细胞术检测外周血T淋巴细胞亚群CD3＋CD4＋T淋巴细胞、CD3＋CD8＋T淋巴细胞、Treg、Th17、CD3＋CD8－γ干扰素＋（Th1）、CD3＋CD8-IL-4＋（Th2）百分率的变化，采用Bio-Plex Pro human cytokine Group Ⅰ 27-plex panel和Group Ⅱ 21-plex panel测定血清中相关细胞因子[IL-8、IL-18、干扰素诱导蛋白（IP）-10、巨噬细胞炎性蛋白（MIP）-1b]水平的变化。采用Kolmogorov-Smirnov检验行正态性检验，两组间比较应用两独立样本t检验。结果 治疗前，H7N9感染组外周血CD3＋CD4＋T淋巴细胞百分率[（27.90±10.19）%比（38.75±6.78）%，t＝－2.726，P＝0.012]、CD3＋CD8＋T淋巴细胞百分率[（14.82±7.72）%比（22.79±6.12）%，t＝－2.556，P＝0.018]明显低于健康对照组，Th17百分率[（2.64±1.40）%比（0.29±0.21）%，t＝4.668，P〈0.001]、Th2百分率[（2.24±2.00）%比（0.35±0.25）%，t＝2.626，P＝0.014]较健康对照组明显升高。治疗后好转15例（好转组），治疗后外周血CD3＋CD4＋T淋巴细胞[（36.54±9.20）%比（25.86±7.22）%，t＝－3.339，P＝0.007]、CD3＋CD8＋T淋巴细胞百分率[（22.70±5.68）%比（15.08±8.80）%，t＝－2.811，P＝0.017]、Treg百分率[（6.08±1.70）%比（3.26±1.64）%，t＝—6.670，P〈0.001]较治疗前明显升高，Th17百分率[（2.12±1.28）%比（2.76±1.33）%，t＝2.220，P＝0.048]、Th1细胞百分率[（4.00±2.13）%比（6.97±1.81）%，t＝4.407，P＝0.001]较治疗前明显下降；死亡8例（死亡组），治疗前后上述免疫学指标的差异均无统计学意义（均P〉0.05）。治疗前，H7N9感染组IL-8[（23.19±14.35）比（12.78±6.76） ng/L，t＝2.277，P＝0
文摘Avian influenza A（H7N9） virus is one subgroup among the larger group of H7 viruses,which normally circulate among birds.The H7N9 subtype of avian influenza viruses has not been known to infect humans until only recently.On March 31,2013,China confirmed the first three human cases of novel avian influenza A（H7N9）infection in Shanghai and Anhui,two of these patients died.1 As of February 27,2014,367 laboratory-confirmed human cases have been reported from 15 provinces/municipalities in China's Mainland,
