Systemic lupus erythematosus （SLE） is a complex autoimmune disease characterized by the production of autoantibodies to a broad range of self-antigens. Profiling the autoantibody repertoire using array-based technol...Systemic lupus erythematosus （SLE） is a complex autoimmune disease characterized by the production of autoantibodies to a broad range of self-antigens. Profiling the autoantibody repertoire using array-based technology has emerged as a powerful tool for the identification of biomarkers in SLE and other autoimmune diseases. Proteomic microarray has the capacity to hold large number of self-antigens on a solid surface and serve as a high-throughput screening method for the determination of autoantibody specificities. The autoantigen arrays carrying a wide variety of self-antigens, such as cell nuclear components （nucleic acids and associated proteins）, cytoplas- mic proteins, phospholipid proteins, cell matrix proteins, mucosal/secreted proteins, glomeruli, and other tissue-specific proteins, have been used for screening of autoantibody specificities associated with different manifestations of SLE. Arrays containing synthetic peptides and molecular modified proteins are also being utilized for identification of autoantibodies targeting to special antigenic epi- topes. Different isotypes of autoantibodies, including IgG, IgM, IgA, and IgE, as well as other Ig subtypes, can be detected simultaneously with multi-color labeled secondary antibodies. Serum and plasma are the most common biologic materials for autoantibody detection, but other body fluids such as cerebrospinal fluid, synovial fluid, and saliva can also be a source of autoantibody detection.展开更多
We analyzed profiles of IgG autoantibodies to 16 cardiac specific proteins and their main immunogenic region B-epitopes, in the groups of already verified cardiac pathology: acute and chronic lymphocytic myocarditis, ...We analyzed profiles of IgG autoantibodies to 16 cardiac specific proteins and their main immunogenic region B-epitopes, in the groups of already verified cardiac pathology: acute and chronic lymphocytic myocarditis, ST elevation myocardial infarction, postinfarction remodeling of myocardium, dilated cardiomyopathy and in healthy controls along with patients, suffered from gastritis (to evaluate immune response against cross-reactive B-epitopes). AAB specific patterns allowed us to distinguish cases among themselves by means of multiparametrical canonical discriminant analysis in approximately 95% of cases. Positive predictive value in the group of MYO reached 95%, in the STEMI—89%, in the PIR—99%, in the DCM—99%, in the group of gastritis—88%. Principal component analysis of mentioned cardiac pathologies extended current clinical knowledge of their immunopathogenesis. Obtained data markedly proved a usability of serum AAB profiling for non invasive screening, differential diagnostics and working hypothesis composition.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.81270852)
文摘Systemic lupus erythematosus （SLE） is a complex autoimmune disease characterized by the production of autoantibodies to a broad range of self-antigens. Profiling the autoantibody repertoire using array-based technology has emerged as a powerful tool for the identification of biomarkers in SLE and other autoimmune diseases. Proteomic microarray has the capacity to hold large number of self-antigens on a solid surface and serve as a high-throughput screening method for the determination of autoantibody specificities. The autoantigen arrays carrying a wide variety of self-antigens, such as cell nuclear components （nucleic acids and associated proteins）, cytoplas- mic proteins, phospholipid proteins, cell matrix proteins, mucosal/secreted proteins, glomeruli, and other tissue-specific proteins, have been used for screening of autoantibody specificities associated with different manifestations of SLE. Arrays containing synthetic peptides and molecular modified proteins are also being utilized for identification of autoantibodies targeting to special antigenic epi- topes. Different isotypes of autoantibodies, including IgG, IgM, IgA, and IgE, as well as other Ig subtypes, can be detected simultaneously with multi-color labeled secondary antibodies. Serum and plasma are the most common biologic materials for autoantibody detection, but other body fluids such as cerebrospinal fluid, synovial fluid, and saliva can also be a source of autoantibody detection.
文摘We analyzed profiles of IgG autoantibodies to 16 cardiac specific proteins and their main immunogenic region B-epitopes, in the groups of already verified cardiac pathology: acute and chronic lymphocytic myocarditis, ST elevation myocardial infarction, postinfarction remodeling of myocardium, dilated cardiomyopathy and in healthy controls along with patients, suffered from gastritis (to evaluate immune response against cross-reactive B-epitopes). AAB specific patterns allowed us to distinguish cases among themselves by means of multiparametrical canonical discriminant analysis in approximately 95% of cases. Positive predictive value in the group of MYO reached 95%, in the STEMI—89%, in the PIR—99%, in the DCM—99%, in the group of gastritis—88%. Principal component analysis of mentioned cardiac pathologies extended current clinical knowledge of their immunopathogenesis. Obtained data markedly proved a usability of serum AAB profiling for non invasive screening, differential diagnostics and working hypothesis composition.
