目的探讨钙调蛋白激酶Ⅱ(CaMKⅡ)和细胞外信号调节激酶(ERK)在孤独症谱系障碍发病中的作用。方法孕12.5 d Sprague-Dawley孕鼠腹腔注射丙戊酸钠600mg/kg建立子代孤独症谱系障碍模型大鼠,对照组注射同等剂量生理盐水。利用HE染色、免疫...目的探讨钙调蛋白激酶Ⅱ(CaMKⅡ)和细胞外信号调节激酶(ERK)在孤独症谱系障碍发病中的作用。方法孕12.5 d Sprague-Dawley孕鼠腹腔注射丙戊酸钠600mg/kg建立子代孤独症谱系障碍模型大鼠,对照组注射同等剂量生理盐水。利用HE染色、免疫组化和图像分析技术观察比较出生后1、7、14d和28d两组大鼠脑部CaMKⅡ、ERK表达情况。结果 HE染色:出生后1d、7d模型组神经元数量较少,出生14d后剧增,出生后28d仍高于对照组。免疫组化:出生后1~14d两组CaMKⅡ、ERK表达均显著升高(P<0.001),出生28d后表达趋于稳定(P>0.05);与对照组相比,模型组各日龄大鼠CaMKⅡ、ERK表达水平均增高(P<0.001);CaMKⅡ、ERK于出生后1d、7d表达显著升高(P<0.001),出生后14d表达量最多(P<0.001),出生28d后趋于稳定(P>0.05)。结论孤独症谱系障碍模型大鼠大脑皮层CaMKⅡ、ERK的表达增加,尤其是在出生后早期。展开更多
The present study describes the occurrence of psychiatric comorbid disorders in a cohort of 86 high functioning autism (HFA)/Asperger syndrome (AS) patients, examined at Child Neurology and Psychiatry Unit of Tor Verg...The present study describes the occurrence of psychiatric comorbid disorders in a cohort of 86 high functioning autism (HFA)/Asperger syndrome (AS) patients, examined at Child Neurology and Psychiatry Unit of Tor Vergata University. 38 patients out of 86 (44.2%) presented one or more psychiatric comorbidities, such as mood disorders, Attention Deficit/Hyperactivity Disorder (ADHD), Tourette syndrome (TS), anxiety disorders, including obsessive-compulsive disorder (OCD), and psychotic symptoms. We compared our sample with the evidences from the scientific literature on psychiatric comorbidity in ASD patient, in particular in HFA/AS. In this paper we focus on the high frequency of comorbid psychiatric disorders in HFA/AS patients, such as mood disorders, Attention Deficit/Hyperactivity Disorder (ADHD), Tourette syndrome (TS), anxiety disorders, including obsessive-compulsive disorder (OCD), and psychosis, including schizophrenia. We analyzed rates of all psichiatric comorbidities diagnosed in a sample of HFA/AS subjects and we compared findings from our study with the evidences from the scientific literature on psychiatric comorbidity in ASD patients, in particular HFA/AS. We point out that comorbid psychiatric symptoms can be hardly diagnosed, because they could present atipically in ASDs then in general population. Furthermore, they could be masked by ASD core symptoms.展开更多
【目的】比较孤独谱系障碍(autism spectrum disorders,ASD)与发育性语言延迟(developmental language disorders,DLD)发病相关因素(风险因素和临床表现)之间的差异。【方法】应用自制发病相关因素调查表对46例ASD患儿和39例DLD患儿进...【目的】比较孤独谱系障碍(autism spectrum disorders,ASD)与发育性语言延迟(developmental language disorders,DLD)发病相关因素(风险因素和临床表现)之间的差异。【方法】应用自制发病相关因素调查表对46例ASD患儿和39例DLD患儿进行回顾性研究。【结果】ASD组与DLD组儿童,开始有意识叫妈妈(或其他带养人)的年龄差异有统计学意义(P=0.01),两组在首次诊断年龄、走路稳年龄、围生期危险因素(早产,胎次,生产方式,窒息缺氧,产重,产伤,头颅外伤,抽搐,重大疾病,带养人,父母生育年龄,家族史)方面差异无统计学意义(P>0.05)。ASD以语言交流障碍为首发症状35例(78.28%),以社会交流障碍为首发症状35例(78.28%),以行为问题(兴趣狭窄和行为刻板重复)为首发症状17例(36.57%),首次诊断平均年龄为39月龄。【结论】ASD患儿语言发育较晚。其核心症状中语言交流障碍和社会交流障碍易被家长察觉,但患儿首次就诊年龄偏大。展开更多
文摘目的探讨钙调蛋白激酶Ⅱ(CaMKⅡ)和细胞外信号调节激酶(ERK)在孤独症谱系障碍发病中的作用。方法孕12.5 d Sprague-Dawley孕鼠腹腔注射丙戊酸钠600mg/kg建立子代孤独症谱系障碍模型大鼠,对照组注射同等剂量生理盐水。利用HE染色、免疫组化和图像分析技术观察比较出生后1、7、14d和28d两组大鼠脑部CaMKⅡ、ERK表达情况。结果 HE染色:出生后1d、7d模型组神经元数量较少,出生14d后剧增,出生后28d仍高于对照组。免疫组化:出生后1~14d两组CaMKⅡ、ERK表达均显著升高(P<0.001),出生28d后表达趋于稳定(P>0.05);与对照组相比,模型组各日龄大鼠CaMKⅡ、ERK表达水平均增高(P<0.001);CaMKⅡ、ERK于出生后1d、7d表达显著升高(P<0.001),出生后14d表达量最多(P<0.001),出生28d后趋于稳定(P>0.05)。结论孤独症谱系障碍模型大鼠大脑皮层CaMKⅡ、ERK的表达增加,尤其是在出生后早期。
文摘The present study describes the occurrence of psychiatric comorbid disorders in a cohort of 86 high functioning autism (HFA)/Asperger syndrome (AS) patients, examined at Child Neurology and Psychiatry Unit of Tor Vergata University. 38 patients out of 86 (44.2%) presented one or more psychiatric comorbidities, such as mood disorders, Attention Deficit/Hyperactivity Disorder (ADHD), Tourette syndrome (TS), anxiety disorders, including obsessive-compulsive disorder (OCD), and psychotic symptoms. We compared our sample with the evidences from the scientific literature on psychiatric comorbidity in ASD patient, in particular in HFA/AS. In this paper we focus on the high frequency of comorbid psychiatric disorders in HFA/AS patients, such as mood disorders, Attention Deficit/Hyperactivity Disorder (ADHD), Tourette syndrome (TS), anxiety disorders, including obsessive-compulsive disorder (OCD), and psychosis, including schizophrenia. We analyzed rates of all psichiatric comorbidities diagnosed in a sample of HFA/AS subjects and we compared findings from our study with the evidences from the scientific literature on psychiatric comorbidity in ASD patients, in particular HFA/AS. We point out that comorbid psychiatric symptoms can be hardly diagnosed, because they could present atipically in ASDs then in general population. Furthermore, they could be masked by ASD core symptoms.
文摘【目的】比较孤独谱系障碍(autism spectrum disorders,ASD)与发育性语言延迟(developmental language disorders,DLD)发病相关因素(风险因素和临床表现)之间的差异。【方法】应用自制发病相关因素调查表对46例ASD患儿和39例DLD患儿进行回顾性研究。【结果】ASD组与DLD组儿童,开始有意识叫妈妈(或其他带养人)的年龄差异有统计学意义(P=0.01),两组在首次诊断年龄、走路稳年龄、围生期危险因素(早产,胎次,生产方式,窒息缺氧,产重,产伤,头颅外伤,抽搐,重大疾病,带养人,父母生育年龄,家族史)方面差异无统计学意义(P>0.05)。ASD以语言交流障碍为首发症状35例(78.28%),以社会交流障碍为首发症状35例(78.28%),以行为问题(兴趣狭窄和行为刻板重复)为首发症状17例(36.57%),首次诊断平均年龄为39月龄。【结论】ASD患儿语言发育较晚。其核心症状中语言交流障碍和社会交流障碍易被家长察觉,但患儿首次就诊年龄偏大。
