The Arabidopsis vacuolar Na+/H+ antiporter gene,AtNHX1,was introduced into soybean by Agrobacterium-mediated transformation.Four independent kanamycin resistant lines were obtained.The result of PCR,Southern blotting ...The Arabidopsis vacuolar Na+/H+ antiporter gene,AtNHX1,was introduced into soybean by Agrobacterium-mediated transformation.Four independent kanamycin resistant lines were obtained.The result of PCR,Southern blotting and Northern blotting analyses demonstrated that the AtNHX1 gene was successfully inserted into the soybean genome and stably expressed in these kanamycin resistant lines.The stability of AtNHX1 expression and salt resistance were evaluated in the soybean transformants for over 6 generations.Two independently derived transgenic lines with high expression level of AtNHX1 were selected,and propagated to generation T5 in the absence of selection pressure.PCR and RT-PCR examinations revealed that AtNHX1 was highly expressed in all investigated transgenic T5 progenies.Furthermore,all transgenic T5 plants showed resistant to salt stress,same as those of homozygous T2 plants.Taken together,our results indicated that constitutive expression of AtNHX1 enhanced salt tolerance in soybean for over 6 generations,suggesting a great potential use of AtNHX1 for improving salt tolerance in plants by genetic engineering.展开更多
基金supported by National Basic Research Program of China (Grant Nos. 2006CB100106 and 2010CB126600)National Natural Science Foundation of China (Grant Nos. 30571196, 0933ZF11C1 and 0933Z411C1)+1 种基金The Ministry of Science and Technology of China (Grant No. 2007AA10Z187)the Key Laboratory of Soybeam Biology in Chinese Education Ministry and Shanghai Key Laboratory of Bio-Energy Crops (Grant No. 08DZ2270800)
文摘The Arabidopsis vacuolar Na+/H+ antiporter gene,AtNHX1,was introduced into soybean by Agrobacterium-mediated transformation.Four independent kanamycin resistant lines were obtained.The result of PCR,Southern blotting and Northern blotting analyses demonstrated that the AtNHX1 gene was successfully inserted into the soybean genome and stably expressed in these kanamycin resistant lines.The stability of AtNHX1 expression and salt resistance were evaluated in the soybean transformants for over 6 generations.Two independently derived transgenic lines with high expression level of AtNHX1 were selected,and propagated to generation T5 in the absence of selection pressure.PCR and RT-PCR examinations revealed that AtNHX1 was highly expressed in all investigated transgenic T5 progenies.Furthermore,all transgenic T5 plants showed resistant to salt stress,same as those of homozygous T2 plants.Taken together,our results indicated that constitutive expression of AtNHX1 enhanced salt tolerance in soybean for over 6 generations,suggesting a great potential use of AtNHX1 for improving salt tolerance in plants by genetic engineering.
