目的探讨重组人促红细胞生成素(recombinant human erythropoietin,rHuEPO)对脑缺血再灌注小鼠水通道蛋白-1(aquaporin1,AQP1)表达的作用。方法 96只昆明小鼠随机分为假手术组(Sham)、rHuEPO治疗组、缺血再灌注组(I/R),每组32只,依据缺...目的探讨重组人促红细胞生成素(recombinant human erythropoietin,rHuEPO)对脑缺血再灌注小鼠水通道蛋白-1(aquaporin1,AQP1)表达的作用。方法 96只昆明小鼠随机分为假手术组(Sham)、rHuEPO治疗组、缺血再灌注组(I/R),每组32只,依据缺血后再灌注不同时间点再细分6h、24h、3d、7d四个小组。利用免疫组织化学方法检测各组小鼠海马组织AQP1的表达;利用Westernblot方法检测各组小鼠海马AQP1的表达。结果免疫组化和Westernblot结果发现,缺血再灌注组AQP1蛋白表达量明显高于假手术组(P<0.05),而rHuEPO治疗组AQP1蛋白表达量则明显少于缺血再灌注组(P<0.05)。结论 rHuEPO能够下调脑缺血再灌注小鼠AQP1的表达。展开更多
OBJECTIVE: To determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats. Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs. METHODS: Th...OBJECTIVE: To determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats. Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs. METHODS: The distribution of AQP1 and AQP5 in alveolar capillary membrane were investigated by immunohistochemistry and immunoelectron microscopy with affinity-purified antibodies to human AQP1 and AQP5. To study the possibility that alveolar capillary membrane AQP1 and AQP5 undergo altered regulation, we established a rat model using alveolar instillation of lipopolysaccharide (LPS). RESULTS: Immunolabelling showed AQP1 was stained primarily in the microvascular endotheli a of normal lungs, while AQP5 was expressed in type I pneumocytes. Immunohisto chemical analysis showed a significant decrease in the expression of AQP1 and AQP5 in injured lungs at 4h-48h after LPS instillation. AQP1 protein was resumed partly at 24h after LPS instillation and steroid administration, whereas AQP5 was unchanged. CONCLUSION: The decreased expressions of AQP1 and AQP5 in injured lungs suggest that both of them may play a role in abnormal fluid transportation.展开更多
文摘目的探讨重组人促红细胞生成素(recombinant human erythropoietin,rHuEPO)对脑缺血再灌注小鼠水通道蛋白-1(aquaporin1,AQP1)表达的作用。方法 96只昆明小鼠随机分为假手术组(Sham)、rHuEPO治疗组、缺血再灌注组(I/R),每组32只,依据缺血后再灌注不同时间点再细分6h、24h、3d、7d四个小组。利用免疫组织化学方法检测各组小鼠海马组织AQP1的表达;利用Westernblot方法检测各组小鼠海马AQP1的表达。结果免疫组化和Westernblot结果发现,缺血再灌注组AQP1蛋白表达量明显高于假手术组(P<0.05),而rHuEPO治疗组AQP1蛋白表达量则明显少于缺血再灌注组(P<0.05)。结论 rHuEPO能够下调脑缺血再灌注小鼠AQP1的表达。
基金ThisprojectwassupportedbytheNationalNaturalScienceFoundationofChina (No 3 9870 3 3 8)
文摘OBJECTIVE: To determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats. Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs. METHODS: The distribution of AQP1 and AQP5 in alveolar capillary membrane were investigated by immunohistochemistry and immunoelectron microscopy with affinity-purified antibodies to human AQP1 and AQP5. To study the possibility that alveolar capillary membrane AQP1 and AQP5 undergo altered regulation, we established a rat model using alveolar instillation of lipopolysaccharide (LPS). RESULTS: Immunolabelling showed AQP1 was stained primarily in the microvascular endotheli a of normal lungs, while AQP5 was expressed in type I pneumocytes. Immunohisto chemical analysis showed a significant decrease in the expression of AQP1 and AQP5 in injured lungs at 4h-48h after LPS instillation. AQP1 protein was resumed partly at 24h after LPS instillation and steroid administration, whereas AQP5 was unchanged. CONCLUSION: The decreased expressions of AQP1 and AQP5 in injured lungs suggest that both of them may play a role in abnormal fluid transportation.
