Objective： Myanmar has a long history of using medicinal plants for treatment of various diseases. To the best of our knowledge there are no previous reports on antiglycation activities of medicinal plants from Myanm...Objective： Myanmar has a long history of using medicinal plants for treatment of various diseases. To the best of our knowledge there are no previous reports on antiglycation activities of medicinal plants from Myanmar. Therefore, this study was aimed to evaluate the antioxidant, antiglycation and antimicrobial properties of 20 ethanolic extracts from 17 medicinal plants indigenous to Myanmar. Methods： In vitro scavenging assays of 2,2-diphenyl-l-picrylhydrazyl （DPPH）, nitric oxide （NO）, superoxide （SO） radicals were used to determine the antioxidant activities. Folin-Ciocalteu＇s method was performed to determine the total phenolic content. Antiglycation and antimicrobial activities were detected by bovine serum albumin-fluorescent assay and agar well diffusion method. Results： Terminalia cbebula Retz. （Fruit）, containing the highest total phenolic content, showed high antioxidant activities with inhibition of 77.98% ± 0.92% 88.95% ± 2.42%, 88.56% ±1.87% and 70.74% ±2.57% for DPPH, NO, SO assays and antiglycation activity respectively. It also showed the antimicrobial activities against Staphylococcus aureus, Bacillus cereus, Escherichia coil, Pseudomonas aeruginosa and Candida albicans with inhibition zone of 19, 18, 17, 25 and 15 ram, respectively. Garcinia mangostana Linn. showed the strongest activities for SO and antiglycation assays with inhibition of 93.68% ± 2.63% and 82.37%± 1.78%. Bark of Melia sp. was the best NO radical scavenger with inhibition rate of 89.39%± 0.60%. Conclusion： The results suggest that these plants are potential sources of antioxidants with free radical-scavenging and antiglycation activities and could be useful for decreasing the oxidative stress and glycation end-product formation in glycation-related diseases.展开更多
研究了海红果渣总黄酮(T FMM)的体外抗氧化作用,测定了T FMM对油脂过氧化、脱氧核糖氧化损伤的抑制作用以及对超氧阴离子(O-2)的清除作用,并与BHT比较。结果显示,T F M M对脂质过氧化以及脱氧核糖氧化损伤有明显的抑制作用,对超氧阴离子...研究了海红果渣总黄酮(T FMM)的体外抗氧化作用,测定了T FMM对油脂过氧化、脱氧核糖氧化损伤的抑制作用以及对超氧阴离子(O-2)的清除作用,并与BHT比较。结果显示,T F M M对脂质过氧化以及脱氧核糖氧化损伤有明显的抑制作用,对超氧阴离子(O-2)有较强的清除作用,效果明显优于同浓度的BHT。且随着海红果总黄酮添加量的增加,对脂质过氧化、脱氧核糖氧化损伤的抑制作用以及超氧阴离子(O-2)的清除抑制作用也随之增强。展开更多
基金financially supported by Biotechnology Research Department, Ministry of Education, Myanmar (Project Grant No. Bio/NPT/20/2013/034)
文摘Objective： Myanmar has a long history of using medicinal plants for treatment of various diseases. To the best of our knowledge there are no previous reports on antiglycation activities of medicinal plants from Myanmar. Therefore, this study was aimed to evaluate the antioxidant, antiglycation and antimicrobial properties of 20 ethanolic extracts from 17 medicinal plants indigenous to Myanmar. Methods： In vitro scavenging assays of 2,2-diphenyl-l-picrylhydrazyl （DPPH）, nitric oxide （NO）, superoxide （SO） radicals were used to determine the antioxidant activities. Folin-Ciocalteu＇s method was performed to determine the total phenolic content. Antiglycation and antimicrobial activities were detected by bovine serum albumin-fluorescent assay and agar well diffusion method. Results： Terminalia cbebula Retz. （Fruit）, containing the highest total phenolic content, showed high antioxidant activities with inhibition of 77.98% ± 0.92% 88.95% ± 2.42%, 88.56% ±1.87% and 70.74% ±2.57% for DPPH, NO, SO assays and antiglycation activity respectively. It also showed the antimicrobial activities against Staphylococcus aureus, Bacillus cereus, Escherichia coil, Pseudomonas aeruginosa and Candida albicans with inhibition zone of 19, 18, 17, 25 and 15 ram, respectively. Garcinia mangostana Linn. showed the strongest activities for SO and antiglycation assays with inhibition of 93.68% ± 2.63% and 82.37%± 1.78%. Bark of Melia sp. was the best NO radical scavenger with inhibition rate of 89.39%± 0.60%. Conclusion： The results suggest that these plants are potential sources of antioxidants with free radical-scavenging and antiglycation activities and could be useful for decreasing the oxidative stress and glycation end-product formation in glycation-related diseases.
文摘研究了海红果渣总黄酮(T FMM)的体外抗氧化作用,测定了T FMM对油脂过氧化、脱氧核糖氧化损伤的抑制作用以及对超氧阴离子(O-2)的清除作用,并与BHT比较。结果显示,T F M M对脂质过氧化以及脱氧核糖氧化损伤有明显的抑制作用,对超氧阴离子(O-2)有较强的清除作用,效果明显优于同浓度的BHT。且随着海红果总黄酮添加量的增加,对脂质过氧化、脱氧核糖氧化损伤的抑制作用以及超氧阴离子(O-2)的清除抑制作用也随之增强。
