为缓解氯胁迫对烟苗生长发育的不利影响,本试验以水凝胶为材料,设置5个处理即CK(不施KCl和水凝胶)、CK1(1.15 g KCl/盆+0 g水凝胶)、T1(1.15 g KCl/盆+1 g水凝胶/盆)、T2(1.15 g KCl/盆+3 g水凝胶/盆)和T3(1.15 g KCl/盆+5 g水凝胶/盆)...为缓解氯胁迫对烟苗生长发育的不利影响,本试验以水凝胶为材料,设置5个处理即CK(不施KCl和水凝胶)、CK1(1.15 g KCl/盆+0 g水凝胶)、T1(1.15 g KCl/盆+1 g水凝胶/盆)、T2(1.15 g KCl/盆+3 g水凝胶/盆)和T3(1.15 g KCl/盆+5 g水凝胶/盆),研究氯胁迫下施用水凝胶对烟苗生长发育、抗氧化系统和常规化学成分的影响。结果表明,随着水凝胶用量增加,烟苗氯含量呈下降趋势,T2、T3处理烟苗氯含量较CK1分别降低21.35%和30.62%;T2处理烟苗地上部地下部鲜重、地上部地下部干重较CK1分别提高16.90%、20.29%、20.00%和30.93%,T3处理烟苗地上部地下部鲜重、地上部地下部干重较CK1分别提高27.70%、22.46%、30.77%和37.11%;T2、T3处理烟苗光合速率较CK1分别提高29.23%、41.78%;T2处理烟苗过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量较CK1分别降低19.30%、22.11%、12.98%和13.25%,T3处理烟苗CAT、POD、SOD活性以及MDA含量较CK1分别降低19.91%、21.59%、21.13%和20.33%;T2、T3处理有效提高氯胁迫下烟苗烟碱、总糖、还原糖含量和钾氯比。综上可见,施用水凝胶可有效缓解氯胁迫对烟苗的不利影响,促进烟苗生长发育。展开更多
The main objective of this study was to investigate the effects of different doses of spermine and its extended supplementation on the morphology, digestive enzyme activities, and intestinal antioxidant capacity in we...The main objective of this study was to investigate the effects of different doses of spermine and its extended supplementation on the morphology, digestive enzyme activities, and intestinal antioxidant capacity in weaning rats. Nineteen-day-old male rats received intragastric spermine at doses of 0.2 and0.4 μmol/g BW for 3 or 7 d, whereas control rats received similar doses of saline. The results are as follows: 1) In the jejunum, the seven-day supplementation with both doses of spermine significantly increased crypt depth(P < 0.05) compared with the control group; the supplementation extension of the high spermine dose increased villus height and crypt depth(P < 0.05); in the ileum, the low spermine dose significantly increased villus height and crypt depth compared with the control group for 7 days(P < 0.05). 2) The 3-day supplementation with high spermine dose increased alkaline phosphatase activity in the jejunum(P < 0.05). 3) In the jejunum, the anti-hydroxyl radical(AHR), total superoxide dismutase(T-SOD), catalase(CAT), and total antioxidant capacity(T-AOC) activities were increased(P < 0.05); however, the malondialdehyde(MDA) content was reduced(P < 0.05) in groups supplemented with the high spermine dose relative to those in the control groups after 3 and 7 d; moreover, the anti-superoxide anion(ASA) and glutathione(GSH) contents increased with the high spermine dose that lasted for 3 days(P < 0.05). Furthermore, the T-SOD and CAT activities(after 3 and 7 d), ASA(after 3 d),and AHR(after 7 d) increased with the high spermine dose compared with those of the low spermine dose(P < 0.05). Extending the supplementation duration(7 d) of the high spermine dose decreased the MDA content and ASA and T-AOC activities(P < 0.05). These results suggested that spermine supplementation can modulate gut development and enhance the antioxidant status of the jejunum in weaning rats, and a dosage of 0.4 μmol spermine/g BW had better effects than the dosage of 0.2 μmol spermine/g BW on accelerating gut development and i展开更多
文摘目的测定藤茶Ampelopsis grossedentata(Hand.-Mazz.)W.T.Wang不同繁殖方式有效成分,分析其抗氧化酶活性。方法采用单因素及正交试验筛选抗氧化酶的最佳提取工艺,测定普通扦插、两段式扦插和组织培养藤茶叶片的抗氧化酶活性;采用溶剂萃取法提取3种繁殖方式中藤茶茎叶的有效成分并测定其含有量。结果藤茶3种繁殖方式中,组培苗多糖含有量最高,两段式扦插苗黄酮和多酚含有量最高;抗氧化酶提取最优工艺为提取液p H 6.0,处理温度20℃,处理时间20 min;抗氧化酶活性依次为两段式扦插苗>普通扦插苗>组培苗。结论考虑到藤茶有效成分含有量和抗氧化酶活性,3种繁殖方法中两段式扦插法最佳。
文摘为缓解氯胁迫对烟苗生长发育的不利影响,本试验以水凝胶为材料,设置5个处理即CK(不施KCl和水凝胶)、CK1(1.15 g KCl/盆+0 g水凝胶)、T1(1.15 g KCl/盆+1 g水凝胶/盆)、T2(1.15 g KCl/盆+3 g水凝胶/盆)和T3(1.15 g KCl/盆+5 g水凝胶/盆),研究氯胁迫下施用水凝胶对烟苗生长发育、抗氧化系统和常规化学成分的影响。结果表明,随着水凝胶用量增加,烟苗氯含量呈下降趋势,T2、T3处理烟苗氯含量较CK1分别降低21.35%和30.62%;T2处理烟苗地上部地下部鲜重、地上部地下部干重较CK1分别提高16.90%、20.29%、20.00%和30.93%,T3处理烟苗地上部地下部鲜重、地上部地下部干重较CK1分别提高27.70%、22.46%、30.77%和37.11%;T2、T3处理烟苗光合速率较CK1分别提高29.23%、41.78%;T2处理烟苗过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量较CK1分别降低19.30%、22.11%、12.98%和13.25%,T3处理烟苗CAT、POD、SOD活性以及MDA含量较CK1分别降低19.91%、21.59%、21.13%和20.33%;T2、T3处理有效提高氯胁迫下烟苗烟碱、总糖、还原糖含量和钾氯比。综上可见,施用水凝胶可有效缓解氯胁迫对烟苗的不利影响,促进烟苗生长发育。
基金financially supported by the National Natural Science Foundation of China (No. 31301986)the Academy of Kechuang Feed Industry in Sichuan and Specific Research Supporting Program for Discipline Construction in Sichuan Agricultural University (to G. Liu)
文摘The main objective of this study was to investigate the effects of different doses of spermine and its extended supplementation on the morphology, digestive enzyme activities, and intestinal antioxidant capacity in weaning rats. Nineteen-day-old male rats received intragastric spermine at doses of 0.2 and0.4 μmol/g BW for 3 or 7 d, whereas control rats received similar doses of saline. The results are as follows: 1) In the jejunum, the seven-day supplementation with both doses of spermine significantly increased crypt depth(P < 0.05) compared with the control group; the supplementation extension of the high spermine dose increased villus height and crypt depth(P < 0.05); in the ileum, the low spermine dose significantly increased villus height and crypt depth compared with the control group for 7 days(P < 0.05). 2) The 3-day supplementation with high spermine dose increased alkaline phosphatase activity in the jejunum(P < 0.05). 3) In the jejunum, the anti-hydroxyl radical(AHR), total superoxide dismutase(T-SOD), catalase(CAT), and total antioxidant capacity(T-AOC) activities were increased(P < 0.05); however, the malondialdehyde(MDA) content was reduced(P < 0.05) in groups supplemented with the high spermine dose relative to those in the control groups after 3 and 7 d; moreover, the anti-superoxide anion(ASA) and glutathione(GSH) contents increased with the high spermine dose that lasted for 3 days(P < 0.05). Furthermore, the T-SOD and CAT activities(after 3 and 7 d), ASA(after 3 d),and AHR(after 7 d) increased with the high spermine dose compared with those of the low spermine dose(P < 0.05). Extending the supplementation duration(7 d) of the high spermine dose decreased the MDA content and ASA and T-AOC activities(P < 0.05). These results suggested that spermine supplementation can modulate gut development and enhance the antioxidant status of the jejunum in weaning rats, and a dosage of 0.4 μmol spermine/g BW had better effects than the dosage of 0.2 μmol spermine/g BW on accelerating gut development and i
