In this study, we evaluated the anti-cancer property of five bark extracts and the isolates from chloroform and ethyl acetate of Annickia chlorantha by the tetrazolium salt method (MTT method). The anti-cancer activit...In this study, we evaluated the anti-cancer property of five bark extracts and the isolates from chloroform and ethyl acetate of Annickia chlorantha by the tetrazolium salt method (MTT method). The anti-cancer activity was performed on human prostate cancer cell lines PC-3 and hormone-dependent breast cancer cell lines MCF-7. Results indicated that the two isolates displayed interesting cytotoxicity towards MCF-7 cell lines with CC<sub>50</sub> of 3.84 CC<sub>50</sub>/mL and 4.87 CC<sub>50</sub>/mL for chloroform and ethyl acetate respectively;while the total bark extracts showed CC<sub>50</sub> of 24.33 CC<sub>50</sub>/mL, 36.49 CC<sub>50</sub>/ mL and 73.52 CC<sub>50</sub>/mL for chloroform, ethyl acetate and methanol extracts respectively. By the other hand on PC-3, the CC<sub>50</sub> of the isolates were higher than the one on MCF-7, more than 10 CC<sub>50</sub>/mL for both chloroform and ethyl acetate isolates and 49.14 CC<sub>50</sub>/mL, 77.33 CC<sub>50</sub>/mL, 89.38 CC<sub>50</sub>/mL and 92.37 CC<sub>50</sub>/mL, respectively for chloroform, ethyl acetate and methanol soluble extracts. From this study, we identified that the two isolates had anti-cancer properties against MCF-7 cell lines.展开更多
文摘In this study, we evaluated the anti-cancer property of five bark extracts and the isolates from chloroform and ethyl acetate of Annickia chlorantha by the tetrazolium salt method (MTT method). The anti-cancer activity was performed on human prostate cancer cell lines PC-3 and hormone-dependent breast cancer cell lines MCF-7. Results indicated that the two isolates displayed interesting cytotoxicity towards MCF-7 cell lines with CC<sub>50</sub> of 3.84 CC<sub>50</sub>/mL and 4.87 CC<sub>50</sub>/mL for chloroform and ethyl acetate respectively;while the total bark extracts showed CC<sub>50</sub> of 24.33 CC<sub>50</sub>/mL, 36.49 CC<sub>50</sub>/ mL and 73.52 CC<sub>50</sub>/mL for chloroform, ethyl acetate and methanol extracts respectively. By the other hand on PC-3, the CC<sub>50</sub> of the isolates were higher than the one on MCF-7, more than 10 CC<sub>50</sub>/mL for both chloroform and ethyl acetate isolates and 49.14 CC<sub>50</sub>/mL, 77.33 CC<sub>50</sub>/mL, 89.38 CC<sub>50</sub>/mL and 92.37 CC<sub>50</sub>/mL, respectively for chloroform, ethyl acetate and methanol soluble extracts. From this study, we identified that the two isolates had anti-cancer properties against MCF-7 cell lines.
