Objective: To discuss the effect of intensity modulated radiation therapy combined with paclitaxel + endostar chemotherapy on serum malignant molecule levels in patients with locally advanced non-small cell lung cance...Objective: To discuss the effect of intensity modulated radiation therapy combined with paclitaxel + endostar chemotherapy on serum malignant molecule levels in patients with locally advanced non-small cell lung cancer. Methods: Patients with locally advanced NSCLC who were treated in the hospital between February 2015 and January 2017 were collected and divided into control group (n=59) and research group (n=59) by random number table. Control group received the routine paclitaxel + endostar chemotherapy after the operation, and research group underwent intensity modulated radiation therapy combined with paclitaxel +endostar chemotherapy after the operation. The differences in serum levels of NSCLC-related tumor markers and angiogenesis indexes were compared between the two groups before and after treatment. Results: Before treatment, the differences in serum levels of NSCLC-related tumor markers and angiogenesis indexes were not statistically significant between the two groups. After treatment, serum TK1, CYFRA21-1, Pro-GRP, CEA, CA125 and SCC-Ag levels of research group were lower than those of control group;serum EGFR, COX-2, VEGF, HIF-1 and MMP-2 levels of research group were lower than those of control group. Conclusion:Postoperative intensity modulated radiation therapy combined with paclitaxel + endostar chemotherapy can effectively reduce the serum malignant molecule levels and optimize the illness in patients with local advanced NSCLC.展开更多
Angiogenesis is a crucial process in the development of inflammatory diseases,including cancer,psoriasis and rheumatoid arthritis.Recently,several alkaloids from Picrasma quassioides had been screened for angiogenic a...Angiogenesis is a crucial process in the development of inflammatory diseases,including cancer,psoriasis and rheumatoid arthritis.Recently,several alkaloids from Picrasma quassioides had been screened for angiogenic activity in the zebrafish model,and the results indicated that 1-methoxycarbony-β-carboline(MCC) could effectively inhibit blood vessel formation.In this study,we further confirmed that MCC can inhibit,in a concentration-dependent manner,the viability,migration,invasion,and tube formation of human umbilical vein endothelial cells(HUVECs) in vitro,as well as the regenerative vascular outgrowth of zebrafish caudal fin in vivo.In the zebrafish xenograft assay,MCC inhibited the growth of tumor masses and the metastatic transplanted DU145 tumor cells.The proteome profile array of the MCC-treated HUVECs showed that MCC could down-regulate several angiogenesis-related self-secreted proteins,including ANG,EGF,b FGF,GRO,IGF-1,PLG and MMP-1.In addition,the expression of two key membrane receptor proteins in angiogenesis,TIE-2 and u PAR,were also down-regulated after MCC treatment.Taken together,these results shed light on the potential therapeutic application of MCC as a potent natural angiogenesis inhibitor via multiple molecular targets.展开更多
Objective Tumors with accelerated growth or high malignancy are thought to undergo active angio- genesis. Whereas by far, there is few study concerning the combination of MIB-1 proliferation index (MIB-1 PI) and tumor...Objective Tumors with accelerated growth or high malignancy are thought to undergo active angio- genesis. Whereas by far, there is few study concerning the combination of MIB-1 proliferation index (MIB-1 PI) and tumor angiogenesis in carcinomas to show their significance in relate to clinicopathological parameters. In the present study, we evaluated the significance or MIB-1 PI and angiogenesis in early stage of gastric cancer. Our focus was es- pecially on the combination of MIB-1 PI and angiogenesis in relate to lymph node metastasis. Method Specimens from 95 patients with early gastric cancer were studied by means or immunohistochemistry using monoclonal MIB-1 and factor Ⅷ related antigen antibodies. Results The mean MIB-1 PI and microvessel count were 22.9% and 34.7, respectively. The MIB-1 PI did not correlate with microvessel count. Both correlated with depth of tumor invasion, lymphatic vessel invasion and lymph node metastasis. Multivarlate analysis showed that combined high MIB-1 PI/hy- pervascularity, as well as lymphatic vessel invasion and tumor size were independent factors that impact on lymph node metastasis. Conclusion A combination of the high MIB-1 PI/hypervascularity is a factor that related to lymph node metastasis in early gastric cancer.展开更多
文摘Objective: To discuss the effect of intensity modulated radiation therapy combined with paclitaxel + endostar chemotherapy on serum malignant molecule levels in patients with locally advanced non-small cell lung cancer. Methods: Patients with locally advanced NSCLC who were treated in the hospital between February 2015 and January 2017 were collected and divided into control group (n=59) and research group (n=59) by random number table. Control group received the routine paclitaxel + endostar chemotherapy after the operation, and research group underwent intensity modulated radiation therapy combined with paclitaxel +endostar chemotherapy after the operation. The differences in serum levels of NSCLC-related tumor markers and angiogenesis indexes were compared between the two groups before and after treatment. Results: Before treatment, the differences in serum levels of NSCLC-related tumor markers and angiogenesis indexes were not statistically significant between the two groups. After treatment, serum TK1, CYFRA21-1, Pro-GRP, CEA, CA125 and SCC-Ag levels of research group were lower than those of control group;serum EGFR, COX-2, VEGF, HIF-1 and MMP-2 levels of research group were lower than those of control group. Conclusion:Postoperative intensity modulated radiation therapy combined with paclitaxel + endostar chemotherapy can effectively reduce the serum malignant molecule levels and optimize the illness in patients with local advanced NSCLC.
基金supported by the National Natural Science Foundation of China(No.81502950)the Fundamental Research Funds for the Central Universities(2015ZD010)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Angiogenesis is a crucial process in the development of inflammatory diseases,including cancer,psoriasis and rheumatoid arthritis.Recently,several alkaloids from Picrasma quassioides had been screened for angiogenic activity in the zebrafish model,and the results indicated that 1-methoxycarbony-β-carboline(MCC) could effectively inhibit blood vessel formation.In this study,we further confirmed that MCC can inhibit,in a concentration-dependent manner,the viability,migration,invasion,and tube formation of human umbilical vein endothelial cells(HUVECs) in vitro,as well as the regenerative vascular outgrowth of zebrafish caudal fin in vivo.In the zebrafish xenograft assay,MCC inhibited the growth of tumor masses and the metastatic transplanted DU145 tumor cells.The proteome profile array of the MCC-treated HUVECs showed that MCC could down-regulate several angiogenesis-related self-secreted proteins,including ANG,EGF,b FGF,GRO,IGF-1,PLG and MMP-1.In addition,the expression of two key membrane receptor proteins in angiogenesis,TIE-2 and u PAR,were also down-regulated after MCC treatment.Taken together,these results shed light on the potential therapeutic application of MCC as a potent natural angiogenesis inhibitor via multiple molecular targets.
文摘Objective Tumors with accelerated growth or high malignancy are thought to undergo active angio- genesis. Whereas by far, there is few study concerning the combination of MIB-1 proliferation index (MIB-1 PI) and tumor angiogenesis in carcinomas to show their significance in relate to clinicopathological parameters. In the present study, we evaluated the significance or MIB-1 PI and angiogenesis in early stage of gastric cancer. Our focus was es- pecially on the combination of MIB-1 PI and angiogenesis in relate to lymph node metastasis. Method Specimens from 95 patients with early gastric cancer were studied by means or immunohistochemistry using monoclonal MIB-1 and factor Ⅷ related antigen antibodies. Results The mean MIB-1 PI and microvessel count were 22.9% and 34.7, respectively. The MIB-1 PI did not correlate with microvessel count. Both correlated with depth of tumor invasion, lymphatic vessel invasion and lymph node metastasis. Multivarlate analysis showed that combined high MIB-1 PI/hy- pervascularity, as well as lymphatic vessel invasion and tumor size were independent factors that impact on lymph node metastasis. Conclusion A combination of the high MIB-1 PI/hypervascularity is a factor that related to lymph node metastasis in early gastric cancer.
