Henoch-Schonlein purpura (HSP) with intestinal perforation and cerebral hemorrhage is a very rare clinical condition. There has been no report of HSP complicated with both intestinal perforation and cerebral hemorrhag...Henoch-Schonlein purpura (HSP) with intestinal perforation and cerebral hemorrhage is a very rare clinical condition. There has been no report of HSP complicated with both intestinal perforation and cerebral hemorrhage until October 2012. Here we describe a case of HSP with intestinal perforation and cerebral hemorrhage in a 5-year-old girl. Plain abdominal radiograph in the erect position showed heavy gas in the right subphrenic space with an elevated diaphragm. Partial resection of the small intestine was performed, and pathological analysis suggested chronic suppurative inflammation in all layers of the ileal wall and mesentery. Seventeen days after surgery, cerebral hemorrhage developed and the patient died.展开更多
Objective:To explore the effect of composite salvia injection(CSI) on platelet parameters in children with anaphylactoid purpura(AP) and its clinical significance.Methods:One hundred and fifty children with AP were as...Objective:To explore the effect of composite salvia injection(CSI) on platelet parameters in children with anaphylactoid purpura(AP) and its clinical significance.Methods:One hundred and fifty children with AP were assigned to two groups,80 in Group A and 70 in Group B.They were treated,respectively,with conventional therapy only or conventional therapy combined with CSI.Their platelet parameters,including blood platelet count(BPC),mean platelet volume(MPV),platelet distribution width(PDW) and plateletcrit(...展开更多
Aims: To study RBL-2H3 cell degranulation phenomena induced by some TCMIs through cell morphological and ultra-structural observation, released enzyme activity and establish RBL-2H3 cell degranulation test indicated ...Aims: To study RBL-2H3 cell degranulation phenomena induced by some TCMIs through cell morphological and ultra-structural observation, released enzyme activity and establish RBL-2H3 cell degranulation test indicated by β- hexosaminidase activity as a method to evaluate TCMIs at nonclinical stage. Methods: RBL-2H3 cells were used to study the degranulation by co-culture with positive control C48/80 and some TCMIs through morphological and ultra-structure observation, β-hexosaminidase activity detection. RBL-2H3 cell degranulation test was established to detect β-hexosaminidase activity caused by 17 kinds of TCMIs and their ingredients. The cytotoxicity effect of some TCMIs on both RBL 2H3 and BRL cells was measured by CCK-8 assay. Results: Toluidine blue staining and ultra-structure of electronic microscope observation of treated RBL-2H3 cells showed degranulation morphologically. Detection of β-hexosaminidase activity in the supernatant of treated cells showed some TCMIs had elevated enzyme release rates. Further analysis of the ingredients and compound in Tanreqing Injection and Shengmai Injection showed Scutellaria baicalensis Georgi in Tanreqing Injection, Red ginseng and Fructus Schisandrae Chinensis in Shengmai Injection were responsible to the degranulation of RBL-2H3 cells. Osmotic pressures and pH influenced RBL-2H3 degranulation. High Osmotic pressure of Tanreqing Injection and low pH of chlorogenic acid at 2.5 and 5.0 mmol/L congcentration might be responsible to high β-hexosaminidase activity. Most of the TCMIs inducing degranulation had cytotoxicity effect for both RBL-2H3 and BRL cells, but some TCMIs inducing degranulation had no cytotoxicity effect. Conclusion: Some TCMIs can induce degranulation of RBL-2H3 cells;RBL-2H3 cell degranulation test can be used in non-clinical stage to detect the risk causing anaphylactoid reactions. Osmotic pressures and pH influenced RBL-2H3 degranulation, and they should be measured before testing. The mechanism of degranulation caused by some TCMIs is cytotoxic展开更多
Severe bronchospasm and anaphylaxis are unanticipated emergencies that may occur in the ambulatory surgery setting. I present a case in which an asthmatic male with nasal congestion has anaphylaxis after induction, wi...Severe bronchospasm and anaphylaxis are unanticipated emergencies that may occur in the ambulatory surgery setting. I present a case in which an asthmatic male with nasal congestion has anaphylaxis after induction, with severe bronchospasm as the primary manifestation. During the course of hospitalization, he was exposed to aspirin and a second episode of severe bronchospasm occurred. He was diagnosed with both anaphylaxis to an anesthetic medication and Aspirin Exacerbated Respiratory Disease, or Samter’s Triad.展开更多
目的:筛选注射用双黄连组方中致敏药材提取物,研究提取物不同萃取部位UPLC指纹图谱与其致类过敏作用相关性,揭示其致类过敏作用的物质基础。方法:以肥大细胞脱颗粒释放的组胺及类胰蛋白酶为药效学指标,筛选金银花、黄芩、连翘致类过敏...目的:筛选注射用双黄连组方中致敏药材提取物,研究提取物不同萃取部位UPLC指纹图谱与其致类过敏作用相关性,揭示其致类过敏作用的物质基础。方法:以肥大细胞脱颗粒释放的组胺及类胰蛋白酶为药效学指标,筛选金银花、黄芩、连翘致类过敏作用的提取物检测,开展对提取物不同萃取部位指纹图谱与组胺释放相关性和注射用双黄连的"指纹图谱与致敏作用相关性"的研究,用LC-MS/MS鉴定,筛选化学单体实验,验证结果合理性。最后再将金银花与注射用双黄连指纹图谱比对考察致敏成分峰归属情况。结果:发现金银花提取物可引起肥大细胞脱颗粒释放组胺、类胰蛋白酶升高引发类过敏反应,将提取物的萃取部位、乙酸乙酯萃取部位的指纹图谱及大鼠组胺浓度的药理指标,用偏最小二乘法(partial least squares method,PLS)分析,有4个峰与组胺释放成正相关,推断为阿魏酸、咖啡酸甲酯及2个未知成分,阿魏酸及咖啡酸甲酯可促大鼠肥大细胞脱颗粒释放组胺;可疑峰5、峰7、峰8、峰9在注射用双黄连指纹图谱中仅化合物I可见。结论:通过注射用双黄连中金银花提取物UPLC指纹图谱与其致类过敏作用PLS法分析,致类过敏作用与其成分有关,部分致敏成分在制剂中降低或清除,本研究为中药注射剂的过敏性物质提供方法学基础。展开更多
文摘Henoch-Schonlein purpura (HSP) with intestinal perforation and cerebral hemorrhage is a very rare clinical condition. There has been no report of HSP complicated with both intestinal perforation and cerebral hemorrhage until October 2012. Here we describe a case of HSP with intestinal perforation and cerebral hemorrhage in a 5-year-old girl. Plain abdominal radiograph in the erect position showed heavy gas in the right subphrenic space with an elevated diaphragm. Partial resection of the small intestine was performed, and pathological analysis suggested chronic suppurative inflammation in all layers of the ileal wall and mesentery. Seventeen days after surgery, cerebral hemorrhage developed and the patient died.
文摘Objective:To explore the effect of composite salvia injection(CSI) on platelet parameters in children with anaphylactoid purpura(AP) and its clinical significance.Methods:One hundred and fifty children with AP were assigned to two groups,80 in Group A and 70 in Group B.They were treated,respectively,with conventional therapy only or conventional therapy combined with CSI.Their platelet parameters,including blood platelet count(BPC),mean platelet volume(MPV),platelet distribution width(PDW) and plateletcrit(...
文摘Aims: To study RBL-2H3 cell degranulation phenomena induced by some TCMIs through cell morphological and ultra-structural observation, released enzyme activity and establish RBL-2H3 cell degranulation test indicated by β- hexosaminidase activity as a method to evaluate TCMIs at nonclinical stage. Methods: RBL-2H3 cells were used to study the degranulation by co-culture with positive control C48/80 and some TCMIs through morphological and ultra-structure observation, β-hexosaminidase activity detection. RBL-2H3 cell degranulation test was established to detect β-hexosaminidase activity caused by 17 kinds of TCMIs and their ingredients. The cytotoxicity effect of some TCMIs on both RBL 2H3 and BRL cells was measured by CCK-8 assay. Results: Toluidine blue staining and ultra-structure of electronic microscope observation of treated RBL-2H3 cells showed degranulation morphologically. Detection of β-hexosaminidase activity in the supernatant of treated cells showed some TCMIs had elevated enzyme release rates. Further analysis of the ingredients and compound in Tanreqing Injection and Shengmai Injection showed Scutellaria baicalensis Georgi in Tanreqing Injection, Red ginseng and Fructus Schisandrae Chinensis in Shengmai Injection were responsible to the degranulation of RBL-2H3 cells. Osmotic pressures and pH influenced RBL-2H3 degranulation. High Osmotic pressure of Tanreqing Injection and low pH of chlorogenic acid at 2.5 and 5.0 mmol/L congcentration might be responsible to high β-hexosaminidase activity. Most of the TCMIs inducing degranulation had cytotoxicity effect for both RBL-2H3 and BRL cells, but some TCMIs inducing degranulation had no cytotoxicity effect. Conclusion: Some TCMIs can induce degranulation of RBL-2H3 cells;RBL-2H3 cell degranulation test can be used in non-clinical stage to detect the risk causing anaphylactoid reactions. Osmotic pressures and pH influenced RBL-2H3 degranulation, and they should be measured before testing. The mechanism of degranulation caused by some TCMIs is cytotoxic
文摘Severe bronchospasm and anaphylaxis are unanticipated emergencies that may occur in the ambulatory surgery setting. I present a case in which an asthmatic male with nasal congestion has anaphylaxis after induction, with severe bronchospasm as the primary manifestation. During the course of hospitalization, he was exposed to aspirin and a second episode of severe bronchospasm occurred. He was diagnosed with both anaphylaxis to an anesthetic medication and Aspirin Exacerbated Respiratory Disease, or Samter’s Triad.
文摘目的:筛选注射用双黄连组方中致敏药材提取物,研究提取物不同萃取部位UPLC指纹图谱与其致类过敏作用相关性,揭示其致类过敏作用的物质基础。方法:以肥大细胞脱颗粒释放的组胺及类胰蛋白酶为药效学指标,筛选金银花、黄芩、连翘致类过敏作用的提取物检测,开展对提取物不同萃取部位指纹图谱与组胺释放相关性和注射用双黄连的"指纹图谱与致敏作用相关性"的研究,用LC-MS/MS鉴定,筛选化学单体实验,验证结果合理性。最后再将金银花与注射用双黄连指纹图谱比对考察致敏成分峰归属情况。结果:发现金银花提取物可引起肥大细胞脱颗粒释放组胺、类胰蛋白酶升高引发类过敏反应,将提取物的萃取部位、乙酸乙酯萃取部位的指纹图谱及大鼠组胺浓度的药理指标,用偏最小二乘法(partial least squares method,PLS)分析,有4个峰与组胺释放成正相关,推断为阿魏酸、咖啡酸甲酯及2个未知成分,阿魏酸及咖啡酸甲酯可促大鼠肥大细胞脱颗粒释放组胺;可疑峰5、峰7、峰8、峰9在注射用双黄连指纹图谱中仅化合物I可见。结论:通过注射用双黄连中金银花提取物UPLC指纹图谱与其致类过敏作用PLS法分析,致类过敏作用与其成分有关,部分致敏成分在制剂中降低或清除,本研究为中药注射剂的过敏性物质提供方法学基础。
