Alternariol caused DNA single-strand breakage. Conversion of the closed circular double-stranded supercoiled DNA (pBR 322) to the nicked circular form and linear form was used to investigate the effect of extracts of ...Alternariol caused DNA single-strand breakage. Conversion of the closed circular double-stranded supercoiled DNA (pBR 322) to the nicked circular form and linear form was used to investigate the effect of extracts of some Chinese medical herbs on DNA nicking induced by alternariol. Some substances in the extracts of Rhizoma polygonati (RP) and Fructus lycii (FL) were shown to protect DNA from the attack by alternariol.Some substance in the RP may bind to plasmid DNA, and this binding reduces the electrophoretic mobility of DNA. These results indicate that substances from FL and RP may be used as DNA protectors. It is possible that they play an important role in preventing cancer.展开更多
The effects of deoxynlvalenol (DON), T--2 toxin, nivalenol (NIv), hutenolide (BuT).alternariol methyl ether(AME) and monlliformin (cON ) on rabbit articular chondrocytes were observed by using the method of chondrocyt...The effects of deoxynlvalenol (DON), T--2 toxin, nivalenol (NIv), hutenolide (BuT).alternariol methyl ether(AME) and monlliformin (cON ) on rabbit articular chondrocytes were observed by using the method of chondrocyte monolayer culture. The amounts or DNA in chondrocytesand glucuronate in matrix were measured. And the chondrocytes were observed by inversion microscope and transmission electron microscope (TEM). The results showed that the cultured chondrocytes were damaged by all the six mycotoxinsl and the synthesis of DNA and the divided reproductionof chondrocytes were restrained; the damage errect was more evident, esl,ecially in the early stage ofculturel the higher concentration or toxin in the media was used, the lower density of the culturalckondrocytes was observed; the cells were even round damaged and dead, so long as the media contolued toxin. When the six mycotoxins arrected the cultural chondrocytes r.spectively, three dirfereut kinds or ultrastructural changes in ckondrocytes were seen by TEa. The relationship betweenmycotoxiu and KBD was preliminarily discussed, and some problems still need further investigation..展开更多
A method for simultaneously analyzing altemariol(AOH), altemariol monomethyl ether (AME) and zearalenone(ZEA) by particle beam LC/MS was established, LC separation was accompiished with a solvent system of methanol a... A method for simultaneously analyzing altemariol(AOH), altemariol monomethyl ether (AME) and zearalenone(ZEA) by particle beam LC/MS was established, LC separation was accompiished with a solvent system of methanol and water (80:20 v/v). The followed particle beam LC/MS analysis gave searchable spectra of AOH. AME and ZEA. Application of this technique to analysis of an alternaria culture confirmed the presence of AOH and AME.展开更多
文摘Alternariol caused DNA single-strand breakage. Conversion of the closed circular double-stranded supercoiled DNA (pBR 322) to the nicked circular form and linear form was used to investigate the effect of extracts of some Chinese medical herbs on DNA nicking induced by alternariol. Some substances in the extracts of Rhizoma polygonati (RP) and Fructus lycii (FL) were shown to protect DNA from the attack by alternariol.Some substance in the RP may bind to plasmid DNA, and this binding reduces the electrophoretic mobility of DNA. These results indicate that substances from FL and RP may be used as DNA protectors. It is possible that they play an important role in preventing cancer.
文摘The effects of deoxynlvalenol (DON), T--2 toxin, nivalenol (NIv), hutenolide (BuT).alternariol methyl ether(AME) and monlliformin (cON ) on rabbit articular chondrocytes were observed by using the method of chondrocyte monolayer culture. The amounts or DNA in chondrocytesand glucuronate in matrix were measured. And the chondrocytes were observed by inversion microscope and transmission electron microscope (TEM). The results showed that the cultured chondrocytes were damaged by all the six mycotoxinsl and the synthesis of DNA and the divided reproductionof chondrocytes were restrained; the damage errect was more evident, esl,ecially in the early stage ofculturel the higher concentration or toxin in the media was used, the lower density of the culturalckondrocytes was observed; the cells were even round damaged and dead, so long as the media contolued toxin. When the six mycotoxins arrected the cultural chondrocytes r.spectively, three dirfereut kinds or ultrastructural changes in ckondrocytes were seen by TEa. The relationship betweenmycotoxiu and KBD was preliminarily discussed, and some problems still need further investigation..
文摘 A method for simultaneously analyzing altemariol(AOH), altemariol monomethyl ether (AME) and zearalenone(ZEA) by particle beam LC/MS was established, LC separation was accompiished with a solvent system of methanol and water (80:20 v/v). The followed particle beam LC/MS analysis gave searchable spectra of AOH. AME and ZEA. Application of this technique to analysis of an alternaria culture confirmed the presence of AOH and AME.
