The paper was to explore the effect of rice blast resistance induced by Ag-antibiotic 702 on antioxidant enzyme activities and malondialdehyde（ MDA）content. At three-leaf and one-heart stage of Luliangyou 996,15 μg...The paper was to explore the effect of rice blast resistance induced by Ag-antibiotic 702 on antioxidant enzyme activities and malondialdehyde（ MDA）content. At three-leaf and one-heart stage of Luliangyou 996,15 μg/mL Ag-antibiotic 702 was sprayed,while Validamycin and distilled water were sprayed as positive control and negative control,respectively. The antioxidant enzyme（ SOD,POD,PPO,CAT and PAL） activities and malondialdehyde（ MDA） content in rice leaves were determined every 24 h within 168 h post spraying. Compared with CK,the MDA content and activities of the five antioxidant enzymes increased in Agantibiotic 702 treatment,Validamycin treatment and M. grisea inoculation treatment. The MDA content and SOD,CAT and POD activities in Ag-antibiotic 702 treatment were significantly higher than those in M. grisea inoculation treatment in early stage,and the APX and LOX activities increased more remarkably and rapidly. The induction effect of Ag-antibiotic 702 could reach or exceed that of Validamycin treatment. Ag-antibiotic 702 enhanced the resistance to rice blast because MDA content and antioxidant enzymes activities were improved in rice leaves. Therefore,the increase of rice blast resistance induced by Ag-antibiotic 702 in rice was positively correlated with the increase of MDA content and antioxidant enzymes activities in rice leaves.展开更多
[Objective] The paper was to explore the effect of rice blast resistance induced by Ag-antibiotics 702 on protein content and chitlnase and β-1,3-glucanase activities in rice. [ Method] At the fourth leaf stage of Lu...[Objective] The paper was to explore the effect of rice blast resistance induced by Ag-antibiotics 702 on protein content and chitlnase and β-1,3-glucanase activities in rice. [ Method] At the fourth leaf stage of Luliangyou 996, 15 μg/mL Ag-antibiotic 702 was sprayed, while Validamycin and distilled wa- ter were sprayed as positive control and negative control, respectively. All treatments were inoculated with spore fluid of Magnaporthe grisea at 48 h post spraying, and the rice inoculated with only distilled water was used as blank control. The enzymes activities （endochitinase, exochitinase and β-1,3-glucanase） and total pro- tein content in rice leaves were determined every 24 h within 168 h post spraying. [ Result] Compared with the blank control, the rice inoculated with spore fluid of M. grisea could significantly increase the total protein content and the activities of β-1,3-glucanase and chitinase. The induction effect of Ag-antibiotics 702 exceeded that of Validamycin treatment. And the changes in activities of β-1,3-glucanase and chitinase had obvious synchronicity. [ Conclusion] Ag-antibiotic 702 can significantly improve the total protein content and the activities of β-1,3-glucanase and chitinase, thus enhancing the resistance to rice blast.展开更多
基金Supported by National Natural Science Foundation of China(31360450)
文摘The paper was to explore the effect of rice blast resistance induced by Ag-antibiotic 702 on antioxidant enzyme activities and malondialdehyde（ MDA）content. At three-leaf and one-heart stage of Luliangyou 996,15 μg/mL Ag-antibiotic 702 was sprayed,while Validamycin and distilled water were sprayed as positive control and negative control,respectively. The antioxidant enzyme（ SOD,POD,PPO,CAT and PAL） activities and malondialdehyde（ MDA） content in rice leaves were determined every 24 h within 168 h post spraying. Compared with CK,the MDA content and activities of the five antioxidant enzymes increased in Agantibiotic 702 treatment,Validamycin treatment and M. grisea inoculation treatment. The MDA content and SOD,CAT and POD activities in Ag-antibiotic 702 treatment were significantly higher than those in M. grisea inoculation treatment in early stage,and the APX and LOX activities increased more remarkably and rapidly. The induction effect of Ag-antibiotic 702 could reach or exceed that of Validamycin treatment. Ag-antibiotic 702 enhanced the resistance to rice blast because MDA content and antioxidant enzymes activities were improved in rice leaves. Therefore,the increase of rice blast resistance induced by Ag-antibiotic 702 in rice was positively correlated with the increase of MDA content and antioxidant enzymes activities in rice leaves.
基金Supported by National Natural Science Foundation of China(31360450)
文摘[Objective] The paper was to explore the effect of rice blast resistance induced by Ag-antibiotics 702 on protein content and chitlnase and β-1,3-glucanase activities in rice. [ Method] At the fourth leaf stage of Luliangyou 996, 15 μg/mL Ag-antibiotic 702 was sprayed, while Validamycin and distilled wa- ter were sprayed as positive control and negative control, respectively. All treatments were inoculated with spore fluid of Magnaporthe grisea at 48 h post spraying, and the rice inoculated with only distilled water was used as blank control. The enzymes activities （endochitinase, exochitinase and β-1,3-glucanase） and total pro- tein content in rice leaves were determined every 24 h within 168 h post spraying. [ Result] Compared with the blank control, the rice inoculated with spore fluid of M. grisea could significantly increase the total protein content and the activities of β-1,3-glucanase and chitinase. The induction effect of Ag-antibiotics 702 exceeded that of Validamycin treatment. And the changes in activities of β-1,3-glucanase and chitinase had obvious synchronicity. [ Conclusion] Ag-antibiotic 702 can significantly improve the total protein content and the activities of β-1,3-glucanase and chitinase, thus enhancing the resistance to rice blast.
