[Objective] The mRNA expression level changes of S-adenosylmethionine synthetase （SAMS） under low temperature stress was studied. [Method] Total RNA were extracted from leaves, stem and earthnut of sweet potato 0,12...[Objective] The mRNA expression level changes of S-adenosylmethionine synthetase （SAMS） under low temperature stress was studied. [Method] Total RNA were extracted from leaves, stem and earthnut of sweet potato 0,12,24,48 and 72 h after low temperature treatement, mRNA expression level was analyzed by reverse expression and Real-time PCR technique. [Result] The expression quality of the gene extracted from leaves, stem and earthnut increased and the expression quality reached the peak point 24,72 and 72 h after low temperature treatment respectively. The expression change of earthnut was the biggest. [Conclusion] Low temperature was good for increasing mRNA expression of relevart genes.展开更多
目的:观察熊去氧胆酸(UDCA)联合S-腺苷蛋氨酸(SAMe)治疗妊娠期轻度肝内胆汁淤积症(ICP)的疗效。方法:选取妊娠期轻度ICP患者213例,按随机数字表法分为联合组(107例)和单药组(106例)。联合组患者采用UDCA联合SAMe治疗,单药组采用UDCA治疗...目的:观察熊去氧胆酸(UDCA)联合S-腺苷蛋氨酸(SAMe)治疗妊娠期轻度肝内胆汁淤积症(ICP)的疗效。方法:选取妊娠期轻度ICP患者213例,按随机数字表法分为联合组(107例)和单药组(106例)。联合组患者采用UDCA联合SAMe治疗,单药组采用UDCA治疗,比较两组患者的疗效及妊娠结局差异。结果:治疗后两组患者的总胆汁酸、肝胆酸、直接胆红素、丙氨酸转氨酶、天冬氨酸转氨酶检测值较治疗前均降低,联合组降低较单药组更加显著,差异均有统计学意义(P<0.05);联合组患者的瘙痒控制时间[(3.8±1.4)d]短于单药组[(5.3±1.5)d],差异有统计学意义(P<0.05);治疗后第3、5天联合组患者的瘙痒程度评分低于单药组患者,差异具有统计学意义(P<0.05);联合组与单药组的胎儿窘迫发生率、新生儿窒息发生率、羊水污染率、剖宫产率、新生儿1 min Apgar评分比较,差异均无统计学意义(P>0.05);联合组患者的早产率为11.22%,显著低于单药组的20.75%,差异具有统计学意义(P<0.05)。结论:UDCA联合SAMe治疗ICP患者较单用UDCA治疗能够更及时地控制患者的临床症状,尽快恢复实验室指标,取得更好的疗效。展开更多
This paper reviews our present knowledge of the role of the one-carbon cycle in mood disorder and schizophrenia with particular attention to S-adenosyl methionine (SAM). After an historical introduction the clinical d...This paper reviews our present knowledge of the role of the one-carbon cycle in mood disorder and schizophrenia with particular attention to S-adenosyl methionine (SAM). After an historical introduction the clinical data is first reviewed of the anti-depressant action of SAM, in particular a survey of double blind placebo-controlled trials. Then follows an account of the biochemical parameters of the action of SAM, in particular the role of folic acid and 5-methyltetrahydrofolate (vitamin B9), polyamines, homocysteine, together with epigenetic studies. In schizophrenia the effect of oral l-methionine on worsening the symptoms of some chronic schizophrenics has been known since 1961. Recent epigenetic research covered has addressed the mechanism of this reaction. This includes the role of SAM in modulating DNA methyltransferase-1 mRNA activity, cytosine 5-methylation, spine numbers and the expression of mRNAs encoding for reelin and GAD67 in GABAergic neurons in the frontal cortex in schizophrenia. There is also evidence that marker D8S542 located within the methionine sulfoxide reductase (MSRA) gene may be involved in schizophrenia as well as 677C > T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene. The possible roles of homocysteine and methionine S-adenosyl transferase kinetics are also discussed.展开更多
Chemically modified cellular co-factors that provide function,such as immobilization or incorporation of fluorescent dyes,are valuable probes of biological activity.A convenient route to obtain S-adenosyl methionine（...Chemically modified cellular co-factors that provide function,such as immobilization or incorporation of fluorescent dyes,are valuable probes of biological activity.A convenient route to obtain S-adenosyl methionine（AdoMet） analogues modified at N-6 adenosine to feature a linker terminating in azide functionality is described herein.Subsequent decoration of such AdoMet analogues with guanidinium terminated linkers leads to novel potential bisubstrate inhibitors for protein arginine methyltrans-ferases, PRMTs.展开更多
Nonalcoholic steatohepatitis(NASH)is an important indication for liver transplantation in many Western countries due to the epidemic of obesity and insulin resistance.Unfortunately,no medication is approved for NASH a...Nonalcoholic steatohepatitis(NASH)is an important indication for liver transplantation in many Western countries due to the epidemic of obesity and insulin resistance.Unfortunately,no medication is approved for NASH and risk factor modification is often advised.Over the last decade,several clinical trials on NASH have been conducted with several ongoing and the future looks promising.Although betaine(trimethyl glycine)was evaluated for NASH,results were mixed in the clinical trials in large part due to the quality of the studies.It seems reasonable to re-evaluate betaine in clinical trials for NASH and alcoholic liver disease due to its low cost,tolerability and mechanism of action.展开更多
文摘[Objective] The mRNA expression level changes of S-adenosylmethionine synthetase （SAMS） under low temperature stress was studied. [Method] Total RNA were extracted from leaves, stem and earthnut of sweet potato 0,12,24,48 and 72 h after low temperature treatement, mRNA expression level was analyzed by reverse expression and Real-time PCR technique. [Result] The expression quality of the gene extracted from leaves, stem and earthnut increased and the expression quality reached the peak point 24,72 and 72 h after low temperature treatment respectively. The expression change of earthnut was the biggest. [Conclusion] Low temperature was good for increasing mRNA expression of relevart genes.
文摘目的:观察熊去氧胆酸(UDCA)联合S-腺苷蛋氨酸(SAMe)治疗妊娠期轻度肝内胆汁淤积症(ICP)的疗效。方法:选取妊娠期轻度ICP患者213例,按随机数字表法分为联合组(107例)和单药组(106例)。联合组患者采用UDCA联合SAMe治疗,单药组采用UDCA治疗,比较两组患者的疗效及妊娠结局差异。结果:治疗后两组患者的总胆汁酸、肝胆酸、直接胆红素、丙氨酸转氨酶、天冬氨酸转氨酶检测值较治疗前均降低,联合组降低较单药组更加显著,差异均有统计学意义(P<0.05);联合组患者的瘙痒控制时间[(3.8±1.4)d]短于单药组[(5.3±1.5)d],差异有统计学意义(P<0.05);治疗后第3、5天联合组患者的瘙痒程度评分低于单药组患者,差异具有统计学意义(P<0.05);联合组与单药组的胎儿窘迫发生率、新生儿窒息发生率、羊水污染率、剖宫产率、新生儿1 min Apgar评分比较,差异均无统计学意义(P>0.05);联合组患者的早产率为11.22%,显著低于单药组的20.75%,差异具有统计学意义(P<0.05)。结论:UDCA联合SAMe治疗ICP患者较单用UDCA治疗能够更及时地控制患者的临床症状,尽快恢复实验室指标,取得更好的疗效。
文摘This paper reviews our present knowledge of the role of the one-carbon cycle in mood disorder and schizophrenia with particular attention to S-adenosyl methionine (SAM). After an historical introduction the clinical data is first reviewed of the anti-depressant action of SAM, in particular a survey of double blind placebo-controlled trials. Then follows an account of the biochemical parameters of the action of SAM, in particular the role of folic acid and 5-methyltetrahydrofolate (vitamin B9), polyamines, homocysteine, together with epigenetic studies. In schizophrenia the effect of oral l-methionine on worsening the symptoms of some chronic schizophrenics has been known since 1961. Recent epigenetic research covered has addressed the mechanism of this reaction. This includes the role of SAM in modulating DNA methyltransferase-1 mRNA activity, cytosine 5-methylation, spine numbers and the expression of mRNAs encoding for reelin and GAD67 in GABAergic neurons in the frontal cortex in schizophrenia. There is also evidence that marker D8S542 located within the methionine sulfoxide reductase (MSRA) gene may be involved in schizophrenia as well as 677C > T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene. The possible roles of homocysteine and methionine S-adenosyl transferase kinetics are also discussed.
基金financially supported by the Medical Research Council Grant(No.G0700840)
文摘Chemically modified cellular co-factors that provide function,such as immobilization or incorporation of fluorescent dyes,are valuable probes of biological activity.A convenient route to obtain S-adenosyl methionine（AdoMet） analogues modified at N-6 adenosine to feature a linker terminating in azide functionality is described herein.Subsequent decoration of such AdoMet analogues with guanidinium terminated linkers leads to novel potential bisubstrate inhibitors for protein arginine methyltrans-ferases, PRMTs.
文摘Nonalcoholic steatohepatitis(NASH)is an important indication for liver transplantation in many Western countries due to the epidemic of obesity and insulin resistance.Unfortunately,no medication is approved for NASH and risk factor modification is often advised.Over the last decade,several clinical trials on NASH have been conducted with several ongoing and the future looks promising.Although betaine(trimethyl glycine)was evaluated for NASH,results were mixed in the clinical trials in large part due to the quality of the studies.It seems reasonable to re-evaluate betaine in clinical trials for NASH and alcoholic liver disease due to its low cost,tolerability and mechanism of action.
