Ancient whole-genome duplications (WGDs or polyploidy) are prevalent in plants, and some WGDs occurred during the timing of severe global environmental changes. It has been suggested that WGDs may have contributed to ...Ancient whole-genome duplications (WGDs or polyploidy) are prevalent in plants, and some WGDs occurred during the timing of severe global environmental changes. It has been suggested that WGDs may have contributed to plant adaptation. However, this still lacks empirical evidence at the genetic level to support the hypothesis. Here, we investigated the survivors of gene duplicates from multiple ancient WGD events on the major branches of angiosperm phylogeny, and aimed to explore genetic evidence supporting the significance of polyploidy. Duplicated genes co-retained from three waves of independent WGDs (～120 million years ago [Ma], ～66, and <20 Ma) were investigated in 25 selected species. Gene families functioning in low temperature and darkness were commonly retained gene duplicates after the eight independently occurring WGDs in many lineages around the Cretaceous-Paleocene boundary, when the global cooling and darkness were the two main stresses. Moreover, the commonly retained duplicates could be key factors which may have contributed to the robustness of the critical stress-related pathways. In addition, genome-wide transcription factors (TFs) functioning in stresses tend to retain duplicates after waves of WGDs, and the coselected gene duplicates in many lineages may play critical roles during severe environmental stresses. Collectively, these results shed new light on the significant contribution of paleopolyploidy to plant adaptation during global environmental changes in the evolutionary history of angiosperms.展开更多
Dietary anthocyanins are important health-promoting antioxidants that make a major contribution to the quality of fruits. It is intriguing that most tomato cultivars do not produce anthocyanins in fruit. However, the ...Dietary anthocyanins are important health-promoting antioxidants that make a major contribution to the quality of fruits. It is intriguing that most tomato cultivars do not produce anthocyanins in fruit. However, the purple tomato variety Indigo Rose, which has the dominant Aft locus combined with the recessive atv locus from wild tomato species, exhibits light-dependent anthocyanin accumulation in the fruit skin. Here, we report that Aft encodes a functional anthocyanin activator named SlAN2-like, while atv encodes a nonfunctional version of the anthocyanin repressor SlMYBATV. The expression of SlAN2-like is responsive to light, and the functional SlAN2-like can activate the expression of both anthocyanin biosynthetic genes and their regulatory genes, suggesting that SlAN2-like acts as a master regulator in the activation of anthocyanin biosynthesis. We further showed that cultivated tomatoes contain nonfunctional alleles of SlAN2-like and therefore fail to produce anthocyanins. Consistently, expression of a functional SlAN2-like gene driven by the fruit-specific promoter in a tomato cultivar led to the activation of the entire anthocyanin biosynthesis pathway and high-level accumulation of anthocyanins in both the peel and flesh. Taken together, our study exemplifies that efficient engineering of complex metabolic pathways could be achieved through tissue-specific expression of master transcriptional regulators.展开更多
In this paper,the weak pre-orthogonal adaptive Fourier decomposition(W-POAFD)method is applied to solve fractional boundary value problems(FBVPs)in the reproducing kernel Hilbert spaces(RKHSs)W_(0)^(4)[0,1] and W^(1)[...In this paper,the weak pre-orthogonal adaptive Fourier decomposition(W-POAFD)method is applied to solve fractional boundary value problems(FBVPs)in the reproducing kernel Hilbert spaces(RKHSs)W_(0)^(4)[0,1] and W^(1)[0,1].The process of the W-POAFD is as follows:(i)choose a dictionary and implement the pre-orthogonalization to all the dictionary elements;(ii)select points in[0,1]by the weak maximal selection principle to determine the corresponding orthonormalized dictionary elements iteratively;(iii)express the analytical solution as a linear combination of these determined dictionary elements.Convergence properties of numerical solutions are also discussed.The numerical experiments are carried out to illustrate the accuracy and efficiency of W-POAFD for solving FBVPs.展开更多
基金This research was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB31000000).We also thank the start-up funding from State Key Laboratory of System atic and Evolutionary Botany,Institute of Botany,the Chinese Academ y of Sciences.
文摘Ancient whole-genome duplications (WGDs or polyploidy) are prevalent in plants, and some WGDs occurred during the timing of severe global environmental changes. It has been suggested that WGDs may have contributed to plant adaptation. However, this still lacks empirical evidence at the genetic level to support the hypothesis. Here, we investigated the survivors of gene duplicates from multiple ancient WGD events on the major branches of angiosperm phylogeny, and aimed to explore genetic evidence supporting the significance of polyploidy. Duplicated genes co-retained from three waves of independent WGDs (～120 million years ago [Ma], ～66, and <20 Ma) were investigated in 25 selected species. Gene families functioning in low temperature and darkness were commonly retained gene duplicates after the eight independently occurring WGDs in many lineages around the Cretaceous-Paleocene boundary, when the global cooling and darkness were the two main stresses. Moreover, the commonly retained duplicates could be key factors which may have contributed to the robustness of the critical stress-related pathways. In addition, genome-wide transcription factors (TFs) functioning in stresses tend to retain duplicates after waves of WGDs, and the coselected gene duplicates in many lineages may play critical roles during severe environmental stresses. Collectively, these results shed new light on the significant contribution of paleopolyploidy to plant adaptation during global environmental changes in the evolutionary history of angiosperms.
基金This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences,China(XDA24020308)the National Key Research and Development Program of China,China(2016YFD0100500)+1 种基金the Ministry of Agriculture of China,China(2016ZX08009-003-001)he Tai-Shan Scholar Program from Shandong Province(No.tsxk20150901).
文摘Dietary anthocyanins are important health-promoting antioxidants that make a major contribution to the quality of fruits. It is intriguing that most tomato cultivars do not produce anthocyanins in fruit. However, the purple tomato variety Indigo Rose, which has the dominant Aft locus combined with the recessive atv locus from wild tomato species, exhibits light-dependent anthocyanin accumulation in the fruit skin. Here, we report that Aft encodes a functional anthocyanin activator named SlAN2-like, while atv encodes a nonfunctional version of the anthocyanin repressor SlMYBATV. The expression of SlAN2-like is responsive to light, and the functional SlAN2-like can activate the expression of both anthocyanin biosynthetic genes and their regulatory genes, suggesting that SlAN2-like acts as a master regulator in the activation of anthocyanin biosynthesis. We further showed that cultivated tomatoes contain nonfunctional alleles of SlAN2-like and therefore fail to produce anthocyanins. Consistently, expression of a functional SlAN2-like gene driven by the fruit-specific promoter in a tomato cultivar led to the activation of the entire anthocyanin biosynthesis pathway and high-level accumulation of anthocyanins in both the peel and flesh. Taken together, our study exemplifies that efficient engineering of complex metabolic pathways could be achieved through tissue-specific expression of master transcriptional regulators.
基金NITech Grant-in-Aid for Encouragement of Excellent Women Researchers(2023)“気候風土に適応する柔軟な建築内外境域の設計手法に関する研究”(适应气候风土的灵活的建筑内外边界设计手法研究)教育部产学合作协同育人项目“人居环境设计专创融合数字化创新平台建设”(编号:220504555304424)。
基金University of Macao Multi-Year Research Grant Ref.No MYRG2016-00053-FST and MYRG2018-00168-FSTthe Science and Technology Development Fund,Macao SAR FDCT/0123/2018/A3.
文摘In this paper,the weak pre-orthogonal adaptive Fourier decomposition(W-POAFD)method is applied to solve fractional boundary value problems(FBVPs)in the reproducing kernel Hilbert spaces(RKHSs)W_(0)^(4)[0,1] and W^(1)[0,1].The process of the W-POAFD is as follows:(i)choose a dictionary and implement the pre-orthogonalization to all the dictionary elements;(ii)select points in[0,1]by the weak maximal selection principle to determine the corresponding orthonormalized dictionary elements iteratively;(iii)express the analytical solution as a linear combination of these determined dictionary elements.Convergence properties of numerical solutions are also discussed.The numerical experiments are carried out to illustrate the accuracy and efficiency of W-POAFD for solving FBVPs.
