目的研究80岁以上高龄老年骨质疏松患者骨代谢特点及初次输注唑来膦酸注射液后急性期不良反应情况。方法回顾2011年1月-2017年9月在我院就诊的骨质疏松患者216例,按年龄分为高龄老年组(年龄≥80岁)96例、非高龄老年组(65岁≤年龄<80...目的研究80岁以上高龄老年骨质疏松患者骨代谢特点及初次输注唑来膦酸注射液后急性期不良反应情况。方法回顾2011年1月-2017年9月在我院就诊的骨质疏松患者216例,按年龄分为高龄老年组(年龄≥80岁)96例、非高龄老年组(65岁≤年龄<80岁)80例和绝经后非老年组(年龄<65岁绝经后女性)40例。测定并比较各组血钙、血磷、Ⅰ型胶原C-末端肽交联(C-terminal telopeptide of type I collagen,CTX)、Ⅰ型原胶原N-端前肽(procollagen type 1 N-peptide,P1NP)、甲状旁腺素(parathyroid hormone,PTH)、25-羟基维生素D[25 hydroxy vitamin D,25(OH)D]和临床等指标的差异。其中140例患者初次静滴唑来膦酸5 mg治疗,并按年龄分为80岁以上组(80^+组)75例和80岁以下组(80^-组)65例,观察两组输注唑来磷酸后两周内出现急性期不良反应(acute-phase response,APR)的情况。结果非高龄老年组血肌酐水平高于绝经后非老年组(P=0.01);高龄老年组与绝经后非老年组比较,血肌酐偏高(P=0.000),25(OH)D水平偏低(P=0.007),PTH水平偏高(P=0.032)。随着分组平均年龄的增大,CTX与P1NP有下降趋势,但组间比较差异无统计学意义。80^+组的APR总体发生率与80^-组比较差异无统计学意义,其中发热、肌骨痛的发生率在80^+组更低,差异有统计学意义(P<0.05)。结论 80岁以上高龄老年骨质疏松患者骨代谢并非都表现为低转换。初次使用5 mg唑来膦酸静滴,常见的不良反应如发热、肌骨痛的发生率更低。展开更多
A review of the systemic acute phase reaction with major cytokines involved, and the hepatic metabolic changes, negative and positive acute phase proteins (APPs) with function and associated pathology is given. It app...A review of the systemic acute phase reaction with major cytokines involved, and the hepatic metabolic changes, negative and positive acute phase proteins (APPs) with function and associated pathology is given. It appears that APPs represent appropriate analytes for assessment of animal health. Whereas they represent non-specific markers as biological effect reactants, they can be used for assessing nutritional deficits and reactive processes, especially when positive and negative acute phase variables are combined in an index. When such acute phase index is applied to separate healthy animals from animals with some disease, much better results are obtained than with single analytes and statistically acceptable results for culling individual animals may be reached. Unfortunately at present no cheap, comprehensive and easy to use system is available for assessing various acute phase proteins in serum or blood samples at the same time. Protein microarray or fluid phase microchip technology may satisfy this need; and permit simultaneous analysis of numerous analytes in the same small volume sample and enable integration of information derived from systemic reactivity and nutrition with disease specific variables. Applying such technology may help to solve health problems in various countries not only in animal husbandry but also in human populations.展开更多
目的：比较全身麻醉联合硬膜外阻滞麻醉和单纯全身麻醉用于腹腔镜胆囊切除术对患者术中循环和应激反应的影响。方法行腹腔镜胆囊切除术择期手术患者60例，采用随机数字表法分为全身麻醉复合硬膜外阻滞麻醉组（A 组）和全身麻醉组（B 组...目的：比较全身麻醉联合硬膜外阻滞麻醉和单纯全身麻醉用于腹腔镜胆囊切除术对患者术中循环和应激反应的影响。方法行腹腔镜胆囊切除术择期手术患者60例，采用随机数字表法分为全身麻醉复合硬膜外阻滞麻醉组（A 组）和全身麻醉组（B 组），每组30例，比较两组麻醉前5 min（T0）、气腹后15 min （T1）、和停气腹后10 min（T2）的 MAP、HR、C-反应蛋白（CRP）、肾上腺素（E）和去甲肾上腺素（NE）浓度。结果 B 组 T1时的 MAP 和 HR[分别为（95．4±4．6）mmHg、（90．5±12．2）次／min]明显高于 A 组[分别为（87．1±6．0）mmHg、（83．2±13．5）次／min]（t1＝7．81，t2＝6．98，均 P ＜0．05）；B 组 T1、T2时的 E、NE 和 T2的CRP[分别为（242．1±40．5）ng／L、（271．5±31．5）ng／L、（238．0±41．8）ng／L、（265．1±40．6）ng／L、（39．3±3．9）mg／L]均明显高于 A 组[分别为（195．7±28．0）ng／L、（236．9±20．3）ng／L、（191．3±31．8）ng／L、（239．2±34．2）ng／L、（26．3±3．8）mg／L]（t1＝10．02，t2＝8．06，t3＝9．42，t4＝8．69，t5＝9．48，均 P ＜0．05）；A 组术后清醒及拔管时间[分别为（7．2±0．8）min、（11．2±7．3）min]明显短于 B 组[分别为（16．4±0．8）min、（18．8±0．4）min]（t1＝9．81，t2＝9．60，均 P ＜0．05）。结论相对于单纯全麻，全麻复合硬膜外阻滞则能较好地抑制术后应激反应。展开更多
OBJECTIVE: To examine the microvascular pathological characteristics and changes in related injury factors in a rat model of acute blood stasis.METHODS: A total of 75 Sprague-Dawley rats were divided randomly and equa...OBJECTIVE: To examine the microvascular pathological characteristics and changes in related injury factors in a rat model of acute blood stasis.METHODS: A total of 75 Sprague-Dawley rats were divided randomly and equally into a control group and four experimental groups assessed at different times after the induction of stasis(0, 1, 3 or 6 h after stasis)(n = 15). The acute blood stasis model was established through rat tail-vein injection of high-molecular-weight dextran. After Electrocardiograph(ECG) detection at predetermined times(0,1, 3 and 6 h after induction of stasis), the rats were sacrificed and blood and cardiac samples were harvested for analysis. Hematoxylin-eosin(HE) staining and transmission electron microscopy were used for histopathological detection; an enzyme linked immunosorbent assay(ELISA) was used to detect thromboxane B2(TXB2) and 6-keto-prostaglandin F1α(6-Keto-PGF1α) concentrations; a real-time polymerase chain reaction(PCR) reaction system was used to detect intercellular adhesion molecule1(ICAM-1) and vascular cell adhesion molecule1(VCAM-1) m RNA expression; western blotting was used to detect vascular endothelial cadherin(VE-cadherin) protein expression.RESULTS: The ST segment in the ECG showed gradual elevation after induction of stasis and continued elevation at a high level at 3 and 6 h. The HE staining showed changes in myocardial cell necrosis and tissue dissociation after the induction of stasis, along with inflammatory infiltration. Results of transmission electron microscopy showed immediate changes in blood stasis and lumen occlusion in the microvasculature, along with endothelial cell swelling. After the induction of stasis, TXB2 concentrations gradually increased while 6-Keto-PGF1αconcentrations were immediately significantly reduced. The TXB2/6-Keto-PGF1αratio was maintained at a high level. ICAM-1 m RNA expression showed an unstable elevation while VCAM-1 m RNA expression was significantly reduced after the induction of stasis. Compared with the control group, VE-cad展开更多
AIM:To investigate the mechanisms involved in a possible modulator role of interleukin(IL) -6 signalling on CYR61-CTGF-NOV(CCN) 2/connective tissue growth factor(CTGF) expression in hepatocytes(PC) and to look for a r...AIM:To investigate the mechanisms involved in a possible modulator role of interleukin(IL) -6 signalling on CYR61-CTGF-NOV(CCN) 2/connective tissue growth factor(CTGF) expression in hepatocytes(PC) and to look for a relation between serum concentrations of these two parameters in patients with acute inflammation. METHODS:Expression of CCN2/CTGF,p-STAT3,p-Smad 3/1 and p-Smad2 was examined in primary freshly isolated rat or cryo-preserved human PC exposed to various stimuli by Western blotting,electrophoretic mobility shift assay(EMSA) ,reporter-gene-assays and reversetranscriptase polymerase chain reaction. RESULTS:IL-6 strongly down-regulated CCN2/CTGF protein and mRNA expression in PC,enhanceable by extracellular presence of the soluble IL-6 receptor gp80,and supported by an inverse relation between IL-6 and CCN2/CTGF concentrations in patients'sera.The inhi-bition of TGFβ1 driven CCN2/CTGF expression by IL-6 did not involve a modulation of Smad2(and Smad1/3) signalling.However,the STAT3 SH2 domain binding peptide,a selective inhibitor of STAT3 DNA binding activity,counteracted the inhibitory effect of IL-6 on CCN2/CTGF expression much more pronounced than pyrrolidine-dithiocarbamate,an inhibitor primarily of STAT3 phosphorylation.An EMSA confirmed STAT3 binding to the proposed proximal STAT binding site in the CCN2/CTGF promoter. CONCLUSION:CCN2/CTGF is identified as a hepatocellular negative acute phase protein which is downregulated by IL-6 via the STAT3 pathway through interaction on the DNA binding level.展开更多
文摘目的研究80岁以上高龄老年骨质疏松患者骨代谢特点及初次输注唑来膦酸注射液后急性期不良反应情况。方法回顾2011年1月-2017年9月在我院就诊的骨质疏松患者216例,按年龄分为高龄老年组(年龄≥80岁)96例、非高龄老年组(65岁≤年龄<80岁)80例和绝经后非老年组(年龄<65岁绝经后女性)40例。测定并比较各组血钙、血磷、Ⅰ型胶原C-末端肽交联(C-terminal telopeptide of type I collagen,CTX)、Ⅰ型原胶原N-端前肽(procollagen type 1 N-peptide,P1NP)、甲状旁腺素(parathyroid hormone,PTH)、25-羟基维生素D[25 hydroxy vitamin D,25(OH)D]和临床等指标的差异。其中140例患者初次静滴唑来膦酸5 mg治疗,并按年龄分为80岁以上组(80^+组)75例和80岁以下组(80^-组)65例,观察两组输注唑来磷酸后两周内出现急性期不良反应(acute-phase response,APR)的情况。结果非高龄老年组血肌酐水平高于绝经后非老年组(P=0.01);高龄老年组与绝经后非老年组比较,血肌酐偏高(P=0.000),25(OH)D水平偏低(P=0.007),PTH水平偏高(P=0.032)。随着分组平均年龄的增大,CTX与P1NP有下降趋势,但组间比较差异无统计学意义。80^+组的APR总体发生率与80^-组比较差异无统计学意义,其中发热、肌骨痛的发生率在80^+组更低,差异有统计学意义(P<0.05)。结论 80岁以上高龄老年骨质疏松患者骨代谢并非都表现为低转换。初次使用5 mg唑来膦酸静滴,常见的不良反应如发热、肌骨痛的发生率更低。
基金The paper presented at the 28th Seminar on Recent Advances inAnimal Health and Production, University Putra Malaysia, KualaLumpur, Malaysia, March 28th, 2005
文摘A review of the systemic acute phase reaction with major cytokines involved, and the hepatic metabolic changes, negative and positive acute phase proteins (APPs) with function and associated pathology is given. It appears that APPs represent appropriate analytes for assessment of animal health. Whereas they represent non-specific markers as biological effect reactants, they can be used for assessing nutritional deficits and reactive processes, especially when positive and negative acute phase variables are combined in an index. When such acute phase index is applied to separate healthy animals from animals with some disease, much better results are obtained than with single analytes and statistically acceptable results for culling individual animals may be reached. Unfortunately at present no cheap, comprehensive and easy to use system is available for assessing various acute phase proteins in serum or blood samples at the same time. Protein microarray or fluid phase microchip technology may satisfy this need; and permit simultaneous analysis of numerous analytes in the same small volume sample and enable integration of information derived from systemic reactivity and nutrition with disease specific variables. Applying such technology may help to solve health problems in various countries not only in animal husbandry but also in human populations.
文摘目的：比较全身麻醉联合硬膜外阻滞麻醉和单纯全身麻醉用于腹腔镜胆囊切除术对患者术中循环和应激反应的影响。方法行腹腔镜胆囊切除术择期手术患者60例，采用随机数字表法分为全身麻醉复合硬膜外阻滞麻醉组（A 组）和全身麻醉组（B 组），每组30例，比较两组麻醉前5 min（T0）、气腹后15 min （T1）、和停气腹后10 min（T2）的 MAP、HR、C-反应蛋白（CRP）、肾上腺素（E）和去甲肾上腺素（NE）浓度。结果 B 组 T1时的 MAP 和 HR[分别为（95．4±4．6）mmHg、（90．5±12．2）次／min]明显高于 A 组[分别为（87．1±6．0）mmHg、（83．2±13．5）次／min]（t1＝7．81，t2＝6．98，均 P ＜0．05）；B 组 T1、T2时的 E、NE 和 T2的CRP[分别为（242．1±40．5）ng／L、（271．5±31．5）ng／L、（238．0±41．8）ng／L、（265．1±40．6）ng／L、（39．3±3．9）mg／L]均明显高于 A 组[分别为（195．7±28．0）ng／L、（236．9±20．3）ng／L、（191．3±31．8）ng／L、（239．2±34．2）ng／L、（26．3±3．8）mg／L]（t1＝10．02，t2＝8．06，t3＝9．42，t4＝8．69，t5＝9．48，均 P ＜0．05）；A 组术后清醒及拔管时间[分别为（7．2±0．8）min、（11．2±7．3）min]明显短于 B 组[分别为（16．4±0．8）min、（18．8±0．4）min]（t1＝9．81，t2＝9．60，均 P ＜0．05）。结论相对于单纯全麻，全麻复合硬膜外阻滞则能较好地抑制术后应激反应。
基金the National Program on Key Basic Research Project(973 Program,No.2012CB518601)
文摘OBJECTIVE: To examine the microvascular pathological characteristics and changes in related injury factors in a rat model of acute blood stasis.METHODS: A total of 75 Sprague-Dawley rats were divided randomly and equally into a control group and four experimental groups assessed at different times after the induction of stasis(0, 1, 3 or 6 h after stasis)(n = 15). The acute blood stasis model was established through rat tail-vein injection of high-molecular-weight dextran. After Electrocardiograph(ECG) detection at predetermined times(0,1, 3 and 6 h after induction of stasis), the rats were sacrificed and blood and cardiac samples were harvested for analysis. Hematoxylin-eosin(HE) staining and transmission electron microscopy were used for histopathological detection; an enzyme linked immunosorbent assay(ELISA) was used to detect thromboxane B2(TXB2) and 6-keto-prostaglandin F1α(6-Keto-PGF1α) concentrations; a real-time polymerase chain reaction(PCR) reaction system was used to detect intercellular adhesion molecule1(ICAM-1) and vascular cell adhesion molecule1(VCAM-1) m RNA expression; western blotting was used to detect vascular endothelial cadherin(VE-cadherin) protein expression.RESULTS: The ST segment in the ECG showed gradual elevation after induction of stasis and continued elevation at a high level at 3 and 6 h. The HE staining showed changes in myocardial cell necrosis and tissue dissociation after the induction of stasis, along with inflammatory infiltration. Results of transmission electron microscopy showed immediate changes in blood stasis and lumen occlusion in the microvasculature, along with endothelial cell swelling. After the induction of stasis, TXB2 concentrations gradually increased while 6-Keto-PGF1αconcentrations were immediately significantly reduced. The TXB2/6-Keto-PGF1αratio was maintained at a high level. ICAM-1 m RNA expression showed an unstable elevation while VCAM-1 m RNA expression was significantly reduced after the induction of stasis. Compared with the control group, VE-cad
文摘AIM:To investigate the mechanisms involved in a possible modulator role of interleukin(IL) -6 signalling on CYR61-CTGF-NOV(CCN) 2/connective tissue growth factor(CTGF) expression in hepatocytes(PC) and to look for a relation between serum concentrations of these two parameters in patients with acute inflammation. METHODS:Expression of CCN2/CTGF,p-STAT3,p-Smad 3/1 and p-Smad2 was examined in primary freshly isolated rat or cryo-preserved human PC exposed to various stimuli by Western blotting,electrophoretic mobility shift assay(EMSA) ,reporter-gene-assays and reversetranscriptase polymerase chain reaction. RESULTS:IL-6 strongly down-regulated CCN2/CTGF protein and mRNA expression in PC,enhanceable by extracellular presence of the soluble IL-6 receptor gp80,and supported by an inverse relation between IL-6 and CCN2/CTGF concentrations in patients'sera.The inhi-bition of TGFβ1 driven CCN2/CTGF expression by IL-6 did not involve a modulation of Smad2(and Smad1/3) signalling.However,the STAT3 SH2 domain binding peptide,a selective inhibitor of STAT3 DNA binding activity,counteracted the inhibitory effect of IL-6 on CCN2/CTGF expression much more pronounced than pyrrolidine-dithiocarbamate,an inhibitor primarily of STAT3 phosphorylation.An EMSA confirmed STAT3 binding to the proposed proximal STAT binding site in the CCN2/CTGF promoter. CONCLUSION:CCN2/CTGF is identified as a hepatocellular negative acute phase protein which is downregulated by IL-6 via the STAT3 pathway through interaction on the DNA binding level.
