Objective To investigate whether acupoint penetration acupuncture(APA)could regulate chondrocyte autophagy and apoptosis via the PI3K/Akt-mTOR signaling pathway to reduce cartilage degeneration in knee osteoarthritis(...Objective To investigate whether acupoint penetration acupuncture(APA)could regulate chondrocyte autophagy and apoptosis via the PI3K/Akt-mTOR signaling pathway to reduce cartilage degeneration in knee osteoarthritis(KOA)rats.Methods KOA was induced in rats via intra-articular injection of sodium iodoacetate resolution.Forty male Sprague-Dawley rats were randomly assigned to blank control,model,APA,electro-acupuncture(EA),and sham model groups(n=8)and those in the APA and EA groups received their respective therapies.Following completion of the treatment course,histological examinations of cartilage and muscle were conducted.Levels of apoptosis-and autophagy-related factors,including Bax,Bcl-2,mTOR,ULK-1,and Beclin-1 protein,and mRNAs were assessed.Additionally,β-endorphin(β-EP)concentrations in the brain and serum were measured.Results Histological analysis revealed that APA alleviated cartilage and muscle damage compared with the model group.APA inhibited cartilage degeneration by modulating the expression of apoptosis-and autophagy-related proteins and mRNA,thus preventing chondrocyte apoptosis.In the APA group,Bax and mTOR protein levels were significantly lower than those in the model group(both P=.024).Conversely,the Bcl-2 expression level was significantly higher than that in the EA group(P=.035).Additionally,ULK-1 expression was significantly lower than that in the EA group(P=.045).The mRNA level of Bax was significantly higher than that in the blank control group(P<.001).However,Beclin-1 levels were significantly higher than those in both the model and EA groups(both P<.001).ELISA results showed a significant decrease in the concentration ofβ-EP in the brains of the rats in the APA group compared with those in the model group(P=.032).Conclusions APA reduced osteoarthritis-related pain and alleviated cartilage damage by upregulating chondrocyte autophagy and down-regulating apoptosis via signaling pathways involving PI3K/Akt-mTOR in KOA rats.展开更多
目的 探讨中医情绪疗法结合透穴刺法对脑梗死的干预价值。方法 选取2017年10月—2020年10月期间于黑龙江省中医药科学院南岗分院收治的120例脑梗死患者,按照随机数字表法分为常规组、透穴组、联合组,每组各40例。其中常规组常规对症治疗...目的 探讨中医情绪疗法结合透穴刺法对脑梗死的干预价值。方法 选取2017年10月—2020年10月期间于黑龙江省中医药科学院南岗分院收治的120例脑梗死患者,按照随机数字表法分为常规组、透穴组、联合组,每组各40例。其中常规组常规对症治疗,透穴组接受透穴刺法治疗,联合组联合透穴刺法及中医情绪疗法。采用中国脑卒中临床神经功能缺损程度评分量表(CSS)及日常生活活动能力量表(Barthel指数)分别评估患者治疗前后神经功能及日常生活活动能力;采用健康调查简表(the MOS item short from health survey, SF-36)对患者日常生活能力进行评分,并分析3组患者治疗前后免疫功能及炎症因子水平[T淋巴细胞亚群CD4^(+)、CD8^(+)分布及白细胞介素-6(Interleukin 6,IL-6)、肿瘤坏死因子-α(Tumor necorosis fator-a, TNF-α)]。结果 3组患者治疗后CSS评分均较治疗前明显下降,Barthel指数均较治疗前明显升高,差异有统计学意义(P<0.05);联合组治疗后CSS评分较透穴组、常规组明显降低,Barthel指数较透穴组、常规组明显升高,差异有统计学意义(P<0.05)。3组患者治疗后生理功能、生理职能、肌体疼痛、活力、社会功能、情感功能、心理卫生、总体健康评分均较治疗前明显升高,差异有统计学意义(P<0.05),联合组治疗后活力、社会功能、情感功能、心理卫生、总体健康评分均较透穴组及常规组明显升高,差异有统计学意义(P<0.05)。3组患者治疗后CD4^(+)、CD4/CD8水平均较治疗前明显升高,CD8^(+)、IL-6、TNF-α水平均较治疗前明显降低,差异有统计学意义(P<0.05);联合组治疗后CD4^(+)、CD4/CD8水平均较透穴组及常规组明显升高,IL-6、TNF-α水平均较透穴组及常规组明显降低,差异有统计学意义(P<0.05)。结论 中医情绪疗法结合透穴刺法对改善脑梗死患者神经功能,促进患者心理健康、提高生活质量具有较好的临床应用价值�展开更多
In the present study, the therapeutic effect of penetration needling was observed in 120 cases facial paralysis patients and compared with that of the routine needling in another 105 cases. Results showed that though ...In the present study, the therapeutic effect of penetration needling was observed in 120 cases facial paralysis patients and compared with that of the routine needling in another 105 cases. Results showed that though no significant difference was found between these two groups in the therapeutic effect, the acupoints used were fewer in penetration needling group and its effect on tear and posterior auricular pain was superior to that of routine needling.展开更多
目的探讨滋水涵木针法联合头穴透刺治疗卒中后上肢运动功能障碍的疗效。方法选取2020年9月—2022年2月医院治疗的卒中后上肢运动功能障碍患者100例,随机分为对照组(53例,头穴透刺疗法)和观察组(47例,滋水涵木针法联合头穴透刺疗法),对...目的探讨滋水涵木针法联合头穴透刺治疗卒中后上肢运动功能障碍的疗效。方法选取2020年9月—2022年2月医院治疗的卒中后上肢运动功能障碍患者100例,随机分为对照组(53例,头穴透刺疗法)和观察组(47例,滋水涵木针法联合头穴透刺疗法),对比两组治疗后临床疗效、Fugl-Meyer运动功能评分上肢部分(Fugl-Meyer assessment-upper extremity,FMA-UE)评分、改良Barthel指数(modified Barthel index,MBI)评分和沃尔夫运动功能测试(Wolf motor function test,WMFT)评分、Brunnstrom偏瘫上肢肌力分级及运动诱发电位皮质潜伏期(cortical latency,CL)和中枢运动传导时间(central motor conduction time,CMCT)值变化。结果观察组治疗有效率较对照组明显较高(P<0.05);两组治疗后FMA-UE评分、MBI评分和WMFT评分较治疗前均明显升高(P<0.05),且观察组较对照组升高更显著(P<0.05);治疗前两组Brunnstrom偏瘫上肢肌力分级间差异无统计学意义(P>0.05);治疗后两组Brunnstrom偏瘫上肢肌力分级较治疗前显著改善(P<0.05);且观察组与对照组相比较改善更明显(P<0.05);两组治疗后CL和CMCT值较治疗前显著降低(P<0.05);且观察组降低更明显(P<0.05)。结论采用滋水涵木针法联合头穴透刺治疗卒中后上肢运动功能障碍具有较好的疗效,能够改善上肢运动功能,提高上肢肌力。展开更多
目的观察电针透刺和Mulligan动态关节松动术治疗轻中度膝骨关节炎(knee osteoarthfitis,KOA)的临床疗效,探寻安全有效的中西医结合治疗方案。方法将90例来自长沙市中心医院的轻中度KOA患者随机分为电针透刺组、关节松动组和联合组,每组3...目的观察电针透刺和Mulligan动态关节松动术治疗轻中度膝骨关节炎(knee osteoarthfitis,KOA)的临床疗效,探寻安全有效的中西医结合治疗方案。方法将90例来自长沙市中心医院的轻中度KOA患者随机分为电针透刺组、关节松动组和联合组,每组30例。电针透刺组即在透刺基础上连接电针仪,关节松动组采用Mulligan动态关节松动术治疗,联合组即电针透刺结合Mulligan动态关节松动术。各组均每天治疗1次,每周治疗5次,共治疗6周。在治疗前及治疗6周后,通过疼痛视觉模拟评分(visual analogue scale,VAS)、西安大略和麦克马斯特大学骨关节炎指数(Western Ontario and McMaster Universities arthritis index,WOMAC)、Lysholm膝关节功能评估量表、膝关节最大主动关节活动度(active range of motion,AROM)、髌上囊积液及关节间隙角等指标综合评定疗效。结果治疗后,3组患者的VAS、WOMAC评分较治疗前均有下降,Lysholm评分、AROM较治疗前均有提高,关节间隙角较治疗前减小,髌上囊积液量较治疗前减少(P<0.05);联合组的上述指标较电针透刺组、关节松动组改善更明显(P<0.05);电针透刺组在减少髌上囊积液方面优于关节松动组(P<0.05);关节松动组在增加膝关节AROM、缩小关节间隙角方面优于电针透刺组(P<0.05)。结论电针透刺及Mulligan动态关节松动术均能不同程度缓解轻中度KOA患者疼痛症状,降低髌上囊积液量,缩小关节间隙角,改善膝关节整体功能,且二者联合疗效更佳。展开更多
基金supported by the Startup Fund Project for Doctor Research,the First Affiliated Hospital of Henan University of Chinese Medicine in 2020(KY-B0354-14).
文摘Objective To investigate whether acupoint penetration acupuncture(APA)could regulate chondrocyte autophagy and apoptosis via the PI3K/Akt-mTOR signaling pathway to reduce cartilage degeneration in knee osteoarthritis(KOA)rats.Methods KOA was induced in rats via intra-articular injection of sodium iodoacetate resolution.Forty male Sprague-Dawley rats were randomly assigned to blank control,model,APA,electro-acupuncture(EA),and sham model groups(n=8)and those in the APA and EA groups received their respective therapies.Following completion of the treatment course,histological examinations of cartilage and muscle were conducted.Levels of apoptosis-and autophagy-related factors,including Bax,Bcl-2,mTOR,ULK-1,and Beclin-1 protein,and mRNAs were assessed.Additionally,β-endorphin(β-EP)concentrations in the brain and serum were measured.Results Histological analysis revealed that APA alleviated cartilage and muscle damage compared with the model group.APA inhibited cartilage degeneration by modulating the expression of apoptosis-and autophagy-related proteins and mRNA,thus preventing chondrocyte apoptosis.In the APA group,Bax and mTOR protein levels were significantly lower than those in the model group(both P=.024).Conversely,the Bcl-2 expression level was significantly higher than that in the EA group(P=.035).Additionally,ULK-1 expression was significantly lower than that in the EA group(P=.045).The mRNA level of Bax was significantly higher than that in the blank control group(P<.001).However,Beclin-1 levels were significantly higher than those in both the model and EA groups(both P<.001).ELISA results showed a significant decrease in the concentration ofβ-EP in the brains of the rats in the APA group compared with those in the model group(P=.032).Conclusions APA reduced osteoarthritis-related pain and alleviated cartilage damage by upregulating chondrocyte autophagy and down-regulating apoptosis via signaling pathways involving PI3K/Akt-mTOR in KOA rats.
文摘目的 探讨中医情绪疗法结合透穴刺法对脑梗死的干预价值。方法 选取2017年10月—2020年10月期间于黑龙江省中医药科学院南岗分院收治的120例脑梗死患者,按照随机数字表法分为常规组、透穴组、联合组,每组各40例。其中常规组常规对症治疗,透穴组接受透穴刺法治疗,联合组联合透穴刺法及中医情绪疗法。采用中国脑卒中临床神经功能缺损程度评分量表(CSS)及日常生活活动能力量表(Barthel指数)分别评估患者治疗前后神经功能及日常生活活动能力;采用健康调查简表(the MOS item short from health survey, SF-36)对患者日常生活能力进行评分,并分析3组患者治疗前后免疫功能及炎症因子水平[T淋巴细胞亚群CD4^(+)、CD8^(+)分布及白细胞介素-6(Interleukin 6,IL-6)、肿瘤坏死因子-α(Tumor necorosis fator-a, TNF-α)]。结果 3组患者治疗后CSS评分均较治疗前明显下降,Barthel指数均较治疗前明显升高,差异有统计学意义(P<0.05);联合组治疗后CSS评分较透穴组、常规组明显降低,Barthel指数较透穴组、常规组明显升高,差异有统计学意义(P<0.05)。3组患者治疗后生理功能、生理职能、肌体疼痛、活力、社会功能、情感功能、心理卫生、总体健康评分均较治疗前明显升高,差异有统计学意义(P<0.05),联合组治疗后活力、社会功能、情感功能、心理卫生、总体健康评分均较透穴组及常规组明显升高,差异有统计学意义(P<0.05)。3组患者治疗后CD4^(+)、CD4/CD8水平均较治疗前明显升高,CD8^(+)、IL-6、TNF-α水平均较治疗前明显降低,差异有统计学意义(P<0.05);联合组治疗后CD4^(+)、CD4/CD8水平均较透穴组及常规组明显升高,IL-6、TNF-α水平均较透穴组及常规组明显降低,差异有统计学意义(P<0.05)。结论 中医情绪疗法结合透穴刺法对改善脑梗死患者神经功能,促进患者心理健康、提高生活质量具有较好的临床应用价值�
文摘In the present study, the therapeutic effect of penetration needling was observed in 120 cases facial paralysis patients and compared with that of the routine needling in another 105 cases. Results showed that though no significant difference was found between these two groups in the therapeutic effect, the acupoints used were fewer in penetration needling group and its effect on tear and posterior auricular pain was superior to that of routine needling.
文摘目的探讨滋水涵木针法联合头穴透刺治疗卒中后上肢运动功能障碍的疗效。方法选取2020年9月—2022年2月医院治疗的卒中后上肢运动功能障碍患者100例,随机分为对照组(53例,头穴透刺疗法)和观察组(47例,滋水涵木针法联合头穴透刺疗法),对比两组治疗后临床疗效、Fugl-Meyer运动功能评分上肢部分(Fugl-Meyer assessment-upper extremity,FMA-UE)评分、改良Barthel指数(modified Barthel index,MBI)评分和沃尔夫运动功能测试(Wolf motor function test,WMFT)评分、Brunnstrom偏瘫上肢肌力分级及运动诱发电位皮质潜伏期(cortical latency,CL)和中枢运动传导时间(central motor conduction time,CMCT)值变化。结果观察组治疗有效率较对照组明显较高(P<0.05);两组治疗后FMA-UE评分、MBI评分和WMFT评分较治疗前均明显升高(P<0.05),且观察组较对照组升高更显著(P<0.05);治疗前两组Brunnstrom偏瘫上肢肌力分级间差异无统计学意义(P>0.05);治疗后两组Brunnstrom偏瘫上肢肌力分级较治疗前显著改善(P<0.05);且观察组与对照组相比较改善更明显(P<0.05);两组治疗后CL和CMCT值较治疗前显著降低(P<0.05);且观察组降低更明显(P<0.05)。结论采用滋水涵木针法联合头穴透刺治疗卒中后上肢运动功能障碍具有较好的疗效,能够改善上肢运动功能,提高上肢肌力。
文摘目的观察电针透刺和Mulligan动态关节松动术治疗轻中度膝骨关节炎(knee osteoarthfitis,KOA)的临床疗效,探寻安全有效的中西医结合治疗方案。方法将90例来自长沙市中心医院的轻中度KOA患者随机分为电针透刺组、关节松动组和联合组,每组30例。电针透刺组即在透刺基础上连接电针仪,关节松动组采用Mulligan动态关节松动术治疗,联合组即电针透刺结合Mulligan动态关节松动术。各组均每天治疗1次,每周治疗5次,共治疗6周。在治疗前及治疗6周后,通过疼痛视觉模拟评分(visual analogue scale,VAS)、西安大略和麦克马斯特大学骨关节炎指数(Western Ontario and McMaster Universities arthritis index,WOMAC)、Lysholm膝关节功能评估量表、膝关节最大主动关节活动度(active range of motion,AROM)、髌上囊积液及关节间隙角等指标综合评定疗效。结果治疗后,3组患者的VAS、WOMAC评分较治疗前均有下降,Lysholm评分、AROM较治疗前均有提高,关节间隙角较治疗前减小,髌上囊积液量较治疗前减少(P<0.05);联合组的上述指标较电针透刺组、关节松动组改善更明显(P<0.05);电针透刺组在减少髌上囊积液方面优于关节松动组(P<0.05);关节松动组在增加膝关节AROM、缩小关节间隙角方面优于电针透刺组(P<0.05)。结论电针透刺及Mulligan动态关节松动术均能不同程度缓解轻中度KOA患者疼痛症状,降低髌上囊积液量,缩小关节间隙角,改善膝关节整体功能,且二者联合疗效更佳。
