Objective： To observe the changes in numbers and degranulation status of local mast cells （MCs） of the complementary acupionts, as well as the concentrations of serum histamine and bradykinin after acupuncture at ...Objective： To observe the changes in numbers and degranulation status of local mast cells （MCs） of the complementary acupionts, as well as the concentrations of serum histamine and bradykinin after acupuncture at rat＇s complementary acupionts of Yanglingquan （GB 34） and Yinlingquan （SP 101, for exploring the mechanisms of the synergistic effect of complementary acupionts. Methods： Using random number table method, 40 Wistar rats were randomly divided into a blank control group （group K）, an acupuncture at Yanglingquan （GB 34） group （group A）, an acupuncture at Yinlingquan （SP 9） group （group B）, an acupuncture at Yanglingquan （GB 34） and Yinlingquan （SP 9） group （group AB）, and an acupuncture at points near Yanglingquan （GB 34） and Yinlingquan （SP 9） group （group CD）, 8 rats in each group. Group A received acupuncture at Yanglingquan （GB 34）; group B received acupuncture at Yinlingquan （SP 9）; group AB received acupuncture at Yanglingquan （GB 34） and Yinlingquan （SP 9）; group CD received acupuncture at the control points [points 3 mm away from Yanglingquan （GB 34） and Yinlingquan （SP 9） respectively]. Acupuncture was performed at bilateral points in each group. Rats in group K were fixed using the same method as rats in the other 4 groups without acupuncture stimulation. Needles of 0.35 mm in diameter and 40 mm in length were used for acupuncture. Needle handle was connected to the G6805-]~[ mode electroacupuncture （EA） device after needling qi was obtained, with sparse-dense wave, frequency of 2 Hz/100 Hz and current of i mA to keep the needle handles slightly tremulous while the rats kept quiet. Rats were continuously stimulated by EA for 20 rain each time. Experimental interventions were conducted on the 1st, 3rd, 5th and 7th days when the experiment started, for 4 times in total. Specimens from rats in each group were collected 2 h after the 7th day. The levels of histamine and bradykinin in serum were detected by enzyme-l展开更多
基金supported by Project of Natural Science Foundation of Hebei Province,No.H2014406047Key Program of Science and Technology of Hebei Higher Education,No.ZD20131001~~
文摘Objective： To observe the changes in numbers and degranulation status of local mast cells （MCs） of the complementary acupionts, as well as the concentrations of serum histamine and bradykinin after acupuncture at rat＇s complementary acupionts of Yanglingquan （GB 34） and Yinlingquan （SP 101, for exploring the mechanisms of the synergistic effect of complementary acupionts. Methods： Using random number table method, 40 Wistar rats were randomly divided into a blank control group （group K）, an acupuncture at Yanglingquan （GB 34） group （group A）, an acupuncture at Yinlingquan （SP 9） group （group B）, an acupuncture at Yanglingquan （GB 34） and Yinlingquan （SP 9） group （group AB）, and an acupuncture at points near Yanglingquan （GB 34） and Yinlingquan （SP 9） group （group CD）, 8 rats in each group. Group A received acupuncture at Yanglingquan （GB 34）; group B received acupuncture at Yinlingquan （SP 9）; group AB received acupuncture at Yanglingquan （GB 34） and Yinlingquan （SP 9）; group CD received acupuncture at the control points [points 3 mm away from Yanglingquan （GB 34） and Yinlingquan （SP 9） respectively]. Acupuncture was performed at bilateral points in each group. Rats in group K were fixed using the same method as rats in the other 4 groups without acupuncture stimulation. Needles of 0.35 mm in diameter and 40 mm in length were used for acupuncture. Needle handle was connected to the G6805-]~[ mode electroacupuncture （EA） device after needling qi was obtained, with sparse-dense wave, frequency of 2 Hz/100 Hz and current of i mA to keep the needle handles slightly tremulous while the rats kept quiet. Rats were continuously stimulated by EA for 20 rain each time. Experimental interventions were conducted on the 1st, 3rd, 5th and 7th days when the experiment started, for 4 times in total. Specimens from rats in each group were collected 2 h after the 7th day. The levels of histamine and bradykinin in serum were detected by enzyme-l
