Background: Preeclampsia is reported to complicate 2% - 8% of pregnancies globally and is an important cause of maternal and perinatal morbidity and mortality. The aetiology and pathogenesis are still poorly understoo...Background: Preeclampsia is reported to complicate 2% - 8% of pregnancies globally and is an important cause of maternal and perinatal morbidity and mortality. The aetiology and pathogenesis are still poorly understood and substantial improvement has not been made in the prediction, prevention and treatment of the disease. Objective: To compare the frequency of activated protein C resistance (APC-R) in patients with pre-eclampsia to that of normotensive pregnant women and to determine the correlation between activated protein ratio (APC-ratio) and the severity of pre-eclampsia. Methodology: A cross-sectional study was carried out in 100 pre-eclamptic patients and 100 normotensive pregnant controls. The APC-ratio was determined using the modified activated partial thromboplastin time. Study participants with APC-ratio of less than 2.0 were defined as having APC-R. Data was analyzed using SPSS version 22.0. Results: Mean APC-ratio was significantly lower in pre-eclamptics (2.89 ± 1.70) compared to normotensive pregnant women (3.57 ± 1.06) (p = 0.0008) and the levels were also higher in mild (2.95 ± 1.15) compared to severe pre-eclamptics (2.62 ± 1.14). The frequency of APC-R was 26% among women with pre-eclampsia compared to 4% among normotensive controls (p = 0.000). Among 100 pre-eclamptic women 7 (21.2%) out of 33 with mild pre–eclampsia had APC-R, while 19 (28.4%) out of 67 with severe pre-eclampsia had APC-R. APC-ratio had a significant negative correlation with mean arterial blood pressure (r = −0.324;p = 0.000) and proteinuria (r = −0.379;p = 0.000) among study participants. Conclusion: The frequency of activated protein c resistance is significantly higher in pre-eclamptics compared to normotensive pregnant women and this is more pronounced in those with severe pre-eclampsia compared with those with mild disease. APC-R may therefore be used as a marker of severity in the disease.展开更多
目的:探讨P选择素、抗心磷脂抗体(anticardiolipin antibody,ACA)及活化蛋白C抵抗(activated protein c resistance,APCR)对下肢创伤骨折术后深静脉血栓形成(deep venous thrombosis,DVT)的预测价值。方法:选择接受手术治疗的下肢创伤...目的:探讨P选择素、抗心磷脂抗体(anticardiolipin antibody,ACA)及活化蛋白C抵抗(activated protein c resistance,APCR)对下肢创伤骨折术后深静脉血栓形成(deep venous thrombosis,DVT)的预测价值。方法:选择接受手术治疗的下肢创伤骨折患者,术后第3天抽取患者静脉血,采用酶联免疫吸附法测定P选择素含量和ACA阳性率,采用Dahlback法测定APCR阳性率。按照《深静脉血栓形成的诊断和治疗指南(第三版)》中DVT的诊断标准判断患者术后2周内是否发生DVT,比较DVT组和无DVT组的血清P选择素含量、血清ACA阳性率及血清APCR阳性率。以是否发生DVT为因变量,以血清P选择素、ACA及APCR为自变量,进行多因素Logistic回归分析。结果:共纳入648例患者,其中31例患者术后2周内发生DVT(DVT组),其余617例均未检出DVT(无DVT组)。2组患者的血清P选择素含量、血清ACA阳性率及血清APCR阳性率比较,差异均有统计学意义(t=26.989,P=0.000;χ^2=0.010,P=0.000;χ^2=12.447,P=0.000)。多因素Logistic回归分析结果显示,血清P选择素、ACA及APCR是下肢创伤骨折术后DVT的危险因素(OR=1.219,P=0.019;OR=1.292,P=0.009;OR=2.430,P=0.012)。结论:P选择素、ACA及APCR是下肢创伤骨折术后DVT的危险因素,可作为预测下肢创伤骨折术后DVT的检测指标。展开更多
目的研究抗活化蛋白C(activated protein C resistance,APCR)现象和FV Leiden在新疆正常人群、血栓患者中的发生情况。方法对414例正常体检者(N)和79例脑梗死患者组(CT)及46例心肌梗死患者组(MI),用APCR法进行APCR敏感比(n-APC-SR)(<...目的研究抗活化蛋白C(activated protein C resistance,APCR)现象和FV Leiden在新疆正常人群、血栓患者中的发生情况。方法对414例正常体检者(N)和79例脑梗死患者组(CT)及46例心肌梗死患者组(MI),用APCR法进行APCR敏感比(n-APC-SR)(<0.68)和APCR阳性率(<2.0为阳性)测定,用多聚酶链反应-限制性内切酶长度多态性(PCR-RFLP)分析及DNA序列分析对以上标本APCR阳性者做FV Leiden突变和凝血酶原G20210A位基因突变的检测的分析。结果APCR发生率正常对照组为6.28%,其APCR发生率在哈萨克族、维吾尔族、回族、汉族中分别是12%、8.4%、8.35%及4.8%,正常人APCR发生率在哈萨克族较高,且各少数名族与汉族APCR发生率差异有统计学意义(P<0.05)。脑梗死病例组的APCR发生率为11.39%,心肌梗死病例组的APCR发生率为8.70%;两组间以及两组APCR发生率分别与对照组比较差异有统计学意义(P<0.05)。各组人群中均未检出FV Leiden 1691G→A突变杂合子以及凝血酶原G20210A位基因突变。结论APCR现象在新疆地区正常人少数民族中有较高的发生率,其分布与人种、地域有关。未检出FV Leiden,和凝血酶原G20210A位突变。因此,FV Leiden突变不是新疆地区人群动脉血栓发病的主要危险因素。展开更多
文摘Background: Preeclampsia is reported to complicate 2% - 8% of pregnancies globally and is an important cause of maternal and perinatal morbidity and mortality. The aetiology and pathogenesis are still poorly understood and substantial improvement has not been made in the prediction, prevention and treatment of the disease. Objective: To compare the frequency of activated protein C resistance (APC-R) in patients with pre-eclampsia to that of normotensive pregnant women and to determine the correlation between activated protein ratio (APC-ratio) and the severity of pre-eclampsia. Methodology: A cross-sectional study was carried out in 100 pre-eclamptic patients and 100 normotensive pregnant controls. The APC-ratio was determined using the modified activated partial thromboplastin time. Study participants with APC-ratio of less than 2.0 were defined as having APC-R. Data was analyzed using SPSS version 22.0. Results: Mean APC-ratio was significantly lower in pre-eclamptics (2.89 ± 1.70) compared to normotensive pregnant women (3.57 ± 1.06) (p = 0.0008) and the levels were also higher in mild (2.95 ± 1.15) compared to severe pre-eclamptics (2.62 ± 1.14). The frequency of APC-R was 26% among women with pre-eclampsia compared to 4% among normotensive controls (p = 0.000). Among 100 pre-eclamptic women 7 (21.2%) out of 33 with mild pre–eclampsia had APC-R, while 19 (28.4%) out of 67 with severe pre-eclampsia had APC-R. APC-ratio had a significant negative correlation with mean arterial blood pressure (r = −0.324;p = 0.000) and proteinuria (r = −0.379;p = 0.000) among study participants. Conclusion: The frequency of activated protein c resistance is significantly higher in pre-eclamptics compared to normotensive pregnant women and this is more pronounced in those with severe pre-eclampsia compared with those with mild disease. APC-R may therefore be used as a marker of severity in the disease.
文摘目的:探讨P选择素、抗心磷脂抗体(anticardiolipin antibody,ACA)及活化蛋白C抵抗(activated protein c resistance,APCR)对下肢创伤骨折术后深静脉血栓形成(deep venous thrombosis,DVT)的预测价值。方法:选择接受手术治疗的下肢创伤骨折患者,术后第3天抽取患者静脉血,采用酶联免疫吸附法测定P选择素含量和ACA阳性率,采用Dahlback法测定APCR阳性率。按照《深静脉血栓形成的诊断和治疗指南(第三版)》中DVT的诊断标准判断患者术后2周内是否发生DVT,比较DVT组和无DVT组的血清P选择素含量、血清ACA阳性率及血清APCR阳性率。以是否发生DVT为因变量,以血清P选择素、ACA及APCR为自变量,进行多因素Logistic回归分析。结果:共纳入648例患者,其中31例患者术后2周内发生DVT(DVT组),其余617例均未检出DVT(无DVT组)。2组患者的血清P选择素含量、血清ACA阳性率及血清APCR阳性率比较,差异均有统计学意义(t=26.989,P=0.000;χ^2=0.010,P=0.000;χ^2=12.447,P=0.000)。多因素Logistic回归分析结果显示,血清P选择素、ACA及APCR是下肢创伤骨折术后DVT的危险因素(OR=1.219,P=0.019;OR=1.292,P=0.009;OR=2.430,P=0.012)。结论:P选择素、ACA及APCR是下肢创伤骨折术后DVT的危险因素,可作为预测下肢创伤骨折术后DVT的检测指标。
文摘目的研究抗活化蛋白C(activated protein C resistance,APCR)现象和FV Leiden在新疆正常人群、血栓患者中的发生情况。方法对414例正常体检者(N)和79例脑梗死患者组(CT)及46例心肌梗死患者组(MI),用APCR法进行APCR敏感比(n-APC-SR)(<0.68)和APCR阳性率(<2.0为阳性)测定,用多聚酶链反应-限制性内切酶长度多态性(PCR-RFLP)分析及DNA序列分析对以上标本APCR阳性者做FV Leiden突变和凝血酶原G20210A位基因突变的检测的分析。结果APCR发生率正常对照组为6.28%,其APCR发生率在哈萨克族、维吾尔族、回族、汉族中分别是12%、8.4%、8.35%及4.8%,正常人APCR发生率在哈萨克族较高,且各少数名族与汉族APCR发生率差异有统计学意义(P<0.05)。脑梗死病例组的APCR发生率为11.39%,心肌梗死病例组的APCR发生率为8.70%;两组间以及两组APCR发生率分别与对照组比较差异有统计学意义(P<0.05)。各组人群中均未检出FV Leiden 1691G→A突变杂合子以及凝血酶原G20210A位基因突变。结论APCR现象在新疆地区正常人少数民族中有较高的发生率,其分布与人种、地域有关。未检出FV Leiden,和凝血酶原G20210A位突变。因此,FV Leiden突变不是新疆地区人群动脉血栓发病的主要危险因素。
