Radix achyranthis bidentatae(RAB), a member of the Amaranthaceae family, has been widely used in Traditional Chinese Medicine for1000 s of years. Increasing interest in RAB-derived medicinal has led to the discovery o...Radix achyranthis bidentatae(RAB), a member of the Amaranthaceae family, has been widely used in Traditional Chinese Medicine for1000 s of years. Increasing interest in RAB-derived medicinal has led to the discovery of additional triterpenoid saponins, phytoecdysones,polysaccharides, and many other compounds, as well as investigations into their pharmacology. A large number of pharmacological studies have shown RAB and its active components possess a range of pharmacological activities, including anti-tumor, anti-fertility, anti-senile, and anti?inflammatory effects. This review is an up?to?date summary and synthesis of the uses of RAB from phytochemical and pharmacological perspectives.展开更多
Kidney-tonifying recipe can reduce the accumulation of advanced glycation end products, prevent neuronal degeneration and improve cognitive functions in ovariectomized rats. Radix Achyranthis Bidentatae alcohol extrac...Kidney-tonifying recipe can reduce the accumulation of advanced glycation end products, prevent neuronal degeneration and improve cognitive functions in ovariectomized rats. Radix Achyranthis Bidentatae alcohol extracts may dose-dependently inhibit non-enzymatic saccharification in vitro. This study aimed to examine the effect of Radix Achyranthis Bidentatae on advanced glycation end products and on learning and memory capabilities in ovariectomized rats. Ovariectomized rats were treated with Radix Achyranthis Bidentatae alcohol extracts （containing 1.5 g/kg crude drug） or 0.1% aminoguanidine for 12 weeks and behavioral testing was performed with the Y-electrical maze. This test revealed that Radix Achyranthis Bidentatae and aminoguanidine could improve the learning and memory capabilities of ovariectomized rats. Results of competitive enzyme-linked immunosorbent assay showed that treatment with Radix Achyranthis Bidentatae or aminoguanidine reduced the accumulation of advanced glycation end products in the frontal cortex of ovariectomized rats, while increasing content in the blood and urine. Biochemical tests showed that treatment with Radix Achyranthis Bidentatae or aminoguanidine decreased superoxide dismutase activity in the serum and frontal cortex, and increased serum levels of glutathione peroxidase in ovariectomized rats. In addition there was no apparent effect on malondialdehyde levels. These experimental findings indicate that Radix Achyranthis Bidentatae inhibits production of advanced glycation end products and its accumulation in brain tissue, and improves learning and memory capabilities in ovariectomized rats. These effects may be associated with an anti-oxidative action of the extract.展开更多
Objective: In traditional Chinese medicine, the herbal pair, Radix Achyranthis Bidentatae(RAB) and Eucommiae Cortex(EC), is widely used to treat osteoporosis. Herein, we determined whether this herbal pair can be used...Objective: In traditional Chinese medicine, the herbal pair, Radix Achyranthis Bidentatae(RAB) and Eucommiae Cortex(EC), is widely used to treat osteoporosis. Herein, we determined whether this herbal pair can be used to ameliorate glucocorticoid(GC)-induced osteoporosis(GIOP) and find its optimal dosage in zebrafish.Methods: The characteristics of the aqueous extract of RAB and EC were separately characterized using high-performance liquid chromatography. Osteoporosis was induced in 5-day post-fertilization zebrafish larvae by exposing them to 10 lmol/L dexamethasone(Dex) for 96 h. Seven combinations of different ratios of RAB and EC were co-administered. Treatment efficacy was determined by calculating zebrafish vertebral area and sum brightness, via alizarin red staining, and by detecting alkaline phosphatase(ALP)activity. Multiple regression analysis was conducted to test the optimal dosage ratio.Results: According to the Chinese Pharmacopoeia(2015), b-ecdysone(b-Ecd) is a major bioactive marker in RAB extract, while pinoresinol diglucoside(PDG) is the major marker in EC extract. Both of b-Ecd and PDG content values aligned with the Chinese Pharmacopoeia standards. Treatment with 10 lmol/L Dex reduced zebrafish vertebral area, sum brightness, and ALP activity, but RAB and EC attenuated these effects. Combining 50 mg/m L RAB and 50 mg/m L EC was optimal for preventing GIOP in zebrafish.Reverse transcription-quantitative polymerase chain reaction was used to evaluate the m RNA expression of osteogenesis-related genes. A treatment of 10 lmol/L Dex decreased runt-related transcription factor 2(Runx2), osteogenic protein-1(OP-1), bone c-carboxyglutamic acid-containing protein(BGLAP), and bcatenin levels. This effect was counteracted by RAB and EC co-treatment(P < 0.05). Additionally, the effect of using the two herbal extracts together was better than single-herb treatments separately. These results demonstrated that RAB and EC preserve osteoblast function in the presence of GC. The best mass ratio was 1:1.Conclus展开更多
基金supported by the National Natural Science Foundation of China (grant number 81803690)the Innovative Talents Funding of Heilongjiang University of Chinese Medicine (grant number 2012RCQ20)+5 种基金the Heilongjiang Postdoctoral Innovation Foundation (grant number LBH-Z10020)the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (grant number 2013bs04)the Natural Science Foundation of Heilongjiang Province (grant number H201324)the Specialized Research Fund for the Doctoral Program of Higher Education of China (grant number 20132327120003)the Heilongjiang Postdoctoral Scientific Research Developmental Fund (grant number LBH-Q16210)the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (grant number UNPYSCT-2017219)
文摘Radix achyranthis bidentatae(RAB), a member of the Amaranthaceae family, has been widely used in Traditional Chinese Medicine for1000 s of years. Increasing interest in RAB-derived medicinal has led to the discovery of additional triterpenoid saponins, phytoecdysones,polysaccharides, and many other compounds, as well as investigations into their pharmacology. A large number of pharmacological studies have shown RAB and its active components possess a range of pharmacological activities, including anti-tumor, anti-fertility, anti-senile, and anti?inflammatory effects. This review is an up?to?date summary and synthesis of the uses of RAB from phytochemical and pharmacological perspectives.
基金financially supported by the National Science and Technology Major Project for New Drug Creation Program by the Ministry of Science and Technology No.2009ZX09502-014
文摘Kidney-tonifying recipe can reduce the accumulation of advanced glycation end products, prevent neuronal degeneration and improve cognitive functions in ovariectomized rats. Radix Achyranthis Bidentatae alcohol extracts may dose-dependently inhibit non-enzymatic saccharification in vitro. This study aimed to examine the effect of Radix Achyranthis Bidentatae on advanced glycation end products and on learning and memory capabilities in ovariectomized rats. Ovariectomized rats were treated with Radix Achyranthis Bidentatae alcohol extracts （containing 1.5 g/kg crude drug） or 0.1% aminoguanidine for 12 weeks and behavioral testing was performed with the Y-electrical maze. This test revealed that Radix Achyranthis Bidentatae and aminoguanidine could improve the learning and memory capabilities of ovariectomized rats. Results of competitive enzyme-linked immunosorbent assay showed that treatment with Radix Achyranthis Bidentatae or aminoguanidine reduced the accumulation of advanced glycation end products in the frontal cortex of ovariectomized rats, while increasing content in the blood and urine. Biochemical tests showed that treatment with Radix Achyranthis Bidentatae or aminoguanidine decreased superoxide dismutase activity in the serum and frontal cortex, and increased serum levels of glutathione peroxidase in ovariectomized rats. In addition there was no apparent effect on malondialdehyde levels. These experimental findings indicate that Radix Achyranthis Bidentatae inhibits production of advanced glycation end products and its accumulation in brain tissue, and improves learning and memory capabilities in ovariectomized rats. These effects may be associated with an anti-oxidative action of the extract.
基金funded by the Natural Science Foundation of China(No.81873318)the Program Foundation of Shanghai Health and Family Planning Commission,China(No.2018JP013)+1 种基金the Ph.D.Program Foundation of Shanghai Municipal Education Commission,China(SMEC,No.B201713)the Postgraduate Innovation Program of Shanghai University of TCM(2020JX357)。
文摘Objective: In traditional Chinese medicine, the herbal pair, Radix Achyranthis Bidentatae(RAB) and Eucommiae Cortex(EC), is widely used to treat osteoporosis. Herein, we determined whether this herbal pair can be used to ameliorate glucocorticoid(GC)-induced osteoporosis(GIOP) and find its optimal dosage in zebrafish.Methods: The characteristics of the aqueous extract of RAB and EC were separately characterized using high-performance liquid chromatography. Osteoporosis was induced in 5-day post-fertilization zebrafish larvae by exposing them to 10 lmol/L dexamethasone(Dex) for 96 h. Seven combinations of different ratios of RAB and EC were co-administered. Treatment efficacy was determined by calculating zebrafish vertebral area and sum brightness, via alizarin red staining, and by detecting alkaline phosphatase(ALP)activity. Multiple regression analysis was conducted to test the optimal dosage ratio.Results: According to the Chinese Pharmacopoeia(2015), b-ecdysone(b-Ecd) is a major bioactive marker in RAB extract, while pinoresinol diglucoside(PDG) is the major marker in EC extract. Both of b-Ecd and PDG content values aligned with the Chinese Pharmacopoeia standards. Treatment with 10 lmol/L Dex reduced zebrafish vertebral area, sum brightness, and ALP activity, but RAB and EC attenuated these effects. Combining 50 mg/m L RAB and 50 mg/m L EC was optimal for preventing GIOP in zebrafish.Reverse transcription-quantitative polymerase chain reaction was used to evaluate the m RNA expression of osteogenesis-related genes. A treatment of 10 lmol/L Dex decreased runt-related transcription factor 2(Runx2), osteogenic protein-1(OP-1), bone c-carboxyglutamic acid-containing protein(BGLAP), and bcatenin levels. This effect was counteracted by RAB and EC co-treatment(P < 0.05). Additionally, the effect of using the two herbal extracts together was better than single-herb treatments separately. These results demonstrated that RAB and EC preserve osteoblast function in the presence of GC. The best mass ratio was 1:1.Conclus
