目的:比较冠状动脉结扎术与腹主动脉缩窄术所建立的大鼠慢性心力衰竭(CHF)模型在病理及病理生理学等方面的差别。方法:实验大鼠随机分为4组:正常对照组(假冠状动脉结扎术组S-LCL、假腹主动脉缩窄术组S-AAC)、冠状动脉结扎术组(LCL组)和...目的:比较冠状动脉结扎术与腹主动脉缩窄术所建立的大鼠慢性心力衰竭(CHF)模型在病理及病理生理学等方面的差别。方法:实验大鼠随机分为4组:正常对照组(假冠状动脉结扎术组S-LCL、假腹主动脉缩窄术组S-AAC)、冠状动脉结扎术组(LCL组)和腹主动脉缩窄术组(AAC组),8周后测定血流动力学变化,计算左心室重量/体重比值(LVW/BW)观察心肌组织形态学改变。结果:LVW/BW在LCL组、AAC组均增加。两种CHF模型左室压力上升和下降最大速率(±dp/dt m ax)均较正常对照组降低(P<0.01)。左室收缩压(LV SP)、左室舒张末压(LVEDP)均较正常对照组升高,而且AAC组比LCL组升高更明显。光镜下LCL组梗死区纤维组织替代坏死心肌细胞,大量炎性细胞浸润,非梗死区心肌细胞肥大伴间质显著增生;AAC组心肌细胞肥大,排列紊乱,小血管壁增厚,间质增生伴少量炎细胞浸润。结论:冠脉结扎术和腹主动脉缩窄术均是建立慢性心力衰竭大鼠模型的有效方法。两者的差别是:腹主动脉缩窄术建立的是压力超负荷型CHF模型,冠脉结扎术建立的是低心输出量型CHF模型。展开更多
BACKGROUND： In the perspective of traditional Chinese medicine, few studies have focused on the compound preparations though there are many investigations. The present study was undertaken to investigate the effect o...BACKGROUND： In the perspective of traditional Chinese medicine, few studies have focused on the compound preparations though there are many investigations. The present study was undertaken to investigate the effect of Zhenwu Tang Granule on chronic pressure-overloaded left ventricular hypertrophy in rats.METHODS： The study was performed at the laboratory of Guangzhou Institute of Respiratory Disease. Male SD rats were divided randomly into 3 groups： sham operation group （n=8）, operation group （n=15） and traditional Chinese medicine （TCM） group （n=15）.The model of myocardial hypertrophy was made by gradually constricting the abdominal aorta. Sixteen weeks later, cardiac ultrasonography was performed in all groups in order to ascertain post-operational left ventricular （LV） hypertrophy. And Zhenwu Tang Granule was added at a dose of 12 g/kg in the mixed feedstuff for 8 weeks in the TCM group. In the 24th week, weight, structure as well as function of the heart in each group were measured by high-frequency ultrasonography, and Masson＇s staining was performed on the cardiac muscles. Meanwhile, total collagen volume fraction （CVF-T） and non-coronary vessel collagen volume fraction （CVF-NV） were analyzed.RESULTS： There was an increase in the weight of the heart in the operation group, with the left ventricule dominated （P〈0.05）. The heart was enlarged, with diastolic interventricular septal distance （IVSd） and left ventricular posterior wall distance （LVPWd） dominated （P〈0.01）.There was a significant decrease in the cardiac function （P〈0.05）. The weight （P〈0.01） and volume of the heart decreased in the TCM group compared with the operation group, with IVSd and systolic left ventricular posterior wall dominated （P〈0.01）. And the cardiac function was improved （P〈0.05）. Significant interstitial and collagen hyperplasia was shown in the operation group based on pathological analysis, and various improvements were proved in the TCM group, i.e.展开更多
目的观察坎离颗粒干预对自发性高血压大鼠(SHR)及腹主动脉缩窄大鼠的降压疗效。方法 (1)SPF级10周龄雄性SHR大鼠30只,按随机数字表法随机分为对照组(蒸馏水10 m L/kg)、坎离组(坎离颗粒混悬液6.75 g/kg)、缬沙坦组(缬沙坦混悬液7.2 mg/k...目的观察坎离颗粒干预对自发性高血压大鼠(SHR)及腹主动脉缩窄大鼠的降压疗效。方法 (1)SPF级10周龄雄性SHR大鼠30只,按随机数字表法随机分为对照组(蒸馏水10 m L/kg)、坎离组(坎离颗粒混悬液6.75 g/kg)、缬沙坦组(缬沙坦混悬液7.2 mg/kg),每组10只。12周龄时开始药物干预,共干预12周。(2)SPF级10周龄雄性Wistar大鼠40只,11周龄时,按随机数字表法随机选取10只为假手术组(蒸馏水10 m L/kg),余30只腹主动脉缩窄术后,再次随机分为模型组(蒸馏水10 m L/kg)、坎离组(坎离颗粒混悬液6.75 g/kg)、缬沙坦组(缬沙坦混悬液7.2 mg/kg),每组10只。12周龄时(造模后1周)开始药物干预,共干预12周。分别于治疗前、后4、8、12周测量大鼠血压,各测3次取平均值。结果 SHR血压:(1)与对照组比较,在治疗后第8周、第12周时坎离组血压均明显降低(P<0.01),缬沙坦组治疗第4周、第8周、第12周血压明显降低(P<0.01);与缬沙坦组比较,坎离组各时点血压差异无统计学意义(P>0.05)。(2)与本组治疗前比较,对照组和坎离组在治疗后第4周、第8周、第12周血压均明显增高(P<0.01);缬沙坦组在治疗后8、12周血压均明显增高(P<0.01)。腹主动脉缩窄大鼠血压:(1)造模1、4、8、12周后,模型组明显升高(P<0.01);与模型组比较,坎离组治疗后第8周、第12周血压均明显降低(P<0.01),缬沙坦组治疗后第4、8、12周血压均明显降低(P<0.01);与缬沙坦组比较,治疗后第8周、第12周时,中药组血压较高(P<0.05)。(2)与本组治疗前相比,假手术组血压于治疗后8、12周血压明显增高(P<0.05);模型组血压于治疗后8周、12周血压明显增高(P<0.05);坎离组血压未见明显变化,差异无统计学意义(P>0.05);缬沙坦组血压于治疗后4、8周血压有降低趋势,但差异无统计学意义(P>0.05),至治疗后12周时血压明显降低(P<0.05)。结论坎离颗粒可以显著降低SHR及腹主动脉缩窄大鼠的血压。展开更多
文摘目的:比较冠状动脉结扎术与腹主动脉缩窄术所建立的大鼠慢性心力衰竭(CHF)模型在病理及病理生理学等方面的差别。方法:实验大鼠随机分为4组:正常对照组(假冠状动脉结扎术组S-LCL、假腹主动脉缩窄术组S-AAC)、冠状动脉结扎术组(LCL组)和腹主动脉缩窄术组(AAC组),8周后测定血流动力学变化,计算左心室重量/体重比值(LVW/BW)观察心肌组织形态学改变。结果:LVW/BW在LCL组、AAC组均增加。两种CHF模型左室压力上升和下降最大速率(±dp/dt m ax)均较正常对照组降低(P<0.01)。左室收缩压(LV SP)、左室舒张末压(LVEDP)均较正常对照组升高,而且AAC组比LCL组升高更明显。光镜下LCL组梗死区纤维组织替代坏死心肌细胞,大量炎性细胞浸润,非梗死区心肌细胞肥大伴间质显著增生;AAC组心肌细胞肥大,排列紊乱,小血管壁增厚,间质增生伴少量炎细胞浸润。结论:冠脉结扎术和腹主动脉缩窄术均是建立慢性心力衰竭大鼠模型的有效方法。两者的差别是:腹主动脉缩窄术建立的是压力超负荷型CHF模型,冠脉结扎术建立的是低心输出量型CHF模型。
文摘BACKGROUND： In the perspective of traditional Chinese medicine, few studies have focused on the compound preparations though there are many investigations. The present study was undertaken to investigate the effect of Zhenwu Tang Granule on chronic pressure-overloaded left ventricular hypertrophy in rats.METHODS： The study was performed at the laboratory of Guangzhou Institute of Respiratory Disease. Male SD rats were divided randomly into 3 groups： sham operation group （n=8）, operation group （n=15） and traditional Chinese medicine （TCM） group （n=15）.The model of myocardial hypertrophy was made by gradually constricting the abdominal aorta. Sixteen weeks later, cardiac ultrasonography was performed in all groups in order to ascertain post-operational left ventricular （LV） hypertrophy. And Zhenwu Tang Granule was added at a dose of 12 g/kg in the mixed feedstuff for 8 weeks in the TCM group. In the 24th week, weight, structure as well as function of the heart in each group were measured by high-frequency ultrasonography, and Masson＇s staining was performed on the cardiac muscles. Meanwhile, total collagen volume fraction （CVF-T） and non-coronary vessel collagen volume fraction （CVF-NV） were analyzed.RESULTS： There was an increase in the weight of the heart in the operation group, with the left ventricule dominated （P〈0.05）. The heart was enlarged, with diastolic interventricular septal distance （IVSd） and left ventricular posterior wall distance （LVPWd） dominated （P〈0.01）.There was a significant decrease in the cardiac function （P〈0.05）. The weight （P〈0.01） and volume of the heart decreased in the TCM group compared with the operation group, with IVSd and systolic left ventricular posterior wall dominated （P〈0.01）. And the cardiac function was improved （P〈0.05）. Significant interstitial and collagen hyperplasia was shown in the operation group based on pathological analysis, and various improvements were proved in the TCM group, i.e.
文摘目的观察坎离颗粒干预对自发性高血压大鼠(SHR)及腹主动脉缩窄大鼠的降压疗效。方法 (1)SPF级10周龄雄性SHR大鼠30只,按随机数字表法随机分为对照组(蒸馏水10 m L/kg)、坎离组(坎离颗粒混悬液6.75 g/kg)、缬沙坦组(缬沙坦混悬液7.2 mg/kg),每组10只。12周龄时开始药物干预,共干预12周。(2)SPF级10周龄雄性Wistar大鼠40只,11周龄时,按随机数字表法随机选取10只为假手术组(蒸馏水10 m L/kg),余30只腹主动脉缩窄术后,再次随机分为模型组(蒸馏水10 m L/kg)、坎离组(坎离颗粒混悬液6.75 g/kg)、缬沙坦组(缬沙坦混悬液7.2 mg/kg),每组10只。12周龄时(造模后1周)开始药物干预,共干预12周。分别于治疗前、后4、8、12周测量大鼠血压,各测3次取平均值。结果 SHR血压:(1)与对照组比较,在治疗后第8周、第12周时坎离组血压均明显降低(P<0.01),缬沙坦组治疗第4周、第8周、第12周血压明显降低(P<0.01);与缬沙坦组比较,坎离组各时点血压差异无统计学意义(P>0.05)。(2)与本组治疗前比较,对照组和坎离组在治疗后第4周、第8周、第12周血压均明显增高(P<0.01);缬沙坦组在治疗后8、12周血压均明显增高(P<0.01)。腹主动脉缩窄大鼠血压:(1)造模1、4、8、12周后,模型组明显升高(P<0.01);与模型组比较,坎离组治疗后第8周、第12周血压均明显降低(P<0.01),缬沙坦组治疗后第4、8、12周血压均明显降低(P<0.01);与缬沙坦组比较,治疗后第8周、第12周时,中药组血压较高(P<0.05)。(2)与本组治疗前相比,假手术组血压于治疗后8、12周血压明显增高(P<0.05);模型组血压于治疗后8周、12周血压明显增高(P<0.05);坎离组血压未见明显变化,差异无统计学意义(P>0.05);缬沙坦组血压于治疗后4、8周血压有降低趋势,但差异无统计学意义(P>0.05),至治疗后12周时血压明显降低(P<0.05)。结论坎离颗粒可以显著降低SHR及腹主动脉缩窄大鼠的血压。
