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Petrogenesis of Middle-Eocene granitoids and their Mafic microgranular enclaves in central Urmia-Dokhtar Magmatic Arc(Iran): Evidence for interaction between felsic and mafic magmas 被引量:5
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作者 Kazem Kazemi Ali Kananian +1 位作者 Yilin Xiao Fatemeh Sarjoughian 《Geoscience Frontiers》 SCIE CAS CSCD 2019年第2期705-723,共19页
Whole rock major and trace element geochemistry together with zircon U-Pb ages and Sr-Nd isotope compositions for the Middle Eocene intrusive rocks in the Haji Abad region are presented. The granitoid hosts, including... Whole rock major and trace element geochemistry together with zircon U-Pb ages and Sr-Nd isotope compositions for the Middle Eocene intrusive rocks in the Haji Abad region are presented. The granitoid hosts, including granodiorite and diorite, yielded zircon U-Pb ages with a weighted mean value of 40.0 ± 0.7 Ma for the granodiorite phase. Mafic microgranular enclaves(MMEs) are common in these plutons, and have relatively low SiO_2 contents(53.04-57.08 wt.%) and high Mg#(42.6-60.1), probably reflecting a mantle-derived origin. The host rocks are metaluminous(A/CNK = 0.69-1.03), arc-related calc-alkaline, and I-type in composition, possessing higher SiO_2 contents(59.7-66.77 wt.%) and lower Mg#(38.6-52.2); they are considered a product of partial melting of the mafic lower crust. Chondritenormalized REE patterns of the MMEs and granitoid hosts are characterized by LREE enrichment and show slight negative Eu anomalies(Eu/Eu* = 0.60-0.93). The host granodiorite samples yield(^(87)Sr/^(86)Sr);ratios ranging from 0.70498 to 0.70591,positive eNd(t) values varying from +0.21 to +2.3, and TDM2 ranging from 760 to 909 Ma, which is consistent with that of associated mafic microgranular enclaves(^(87)Sr/^(86)Sr)i = 0.705111-0.705113, ε_(Nd)(t)= +2.14 to +2.16, T_(DM2) = 697-785 Ma). Petrographic and geochemical characterization together with bulk rock Nd-Sr isotopic data suggest that host rocks and associated enclaves originated by interaction between basaltic lower crust-derived felsic and mantlederived mafic magmas in an active continental margin arc environment. 展开更多
关键词 Geochemistry U-Pb geochronology GRANITOID Haji abad Low angle SUBDUCTION Urumieh-Dokhtar MAGMATIC Arc
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Review and Analysis of Geological Structural Model by Using Geomagnetic, Case Study: Haji Abad Region in Iran’s Zagros Zone 被引量:1
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作者 Shima Rahmati Kamel Mahmood Almasian Mohsen Pourkermani 《Open Journal of Geology》 2015年第2期39-54,共16页
The use of geomagnetic for geophysical and geological studies is a new method for receiving different information from new and old faults and lineaments. Accordingly, the present study is applied research and it use c... The use of geomagnetic for geophysical and geological studies is a new method for receiving different information from new and old faults and lineaments. Accordingly, the present study is applied research and it use combined the two methods, analytical signal and Euler’s combined method and invented a new combined method to estimate the depth, location and shape of the magnetic and gravity sources. As a case, this research selected Iran’s Zagros zone. This region due to the geological dynamics is considered by many geologists. In this regard, the 1:250,000 framework of Haji Abad was selected as a case study because of its convenient location for structural analysis. As a result, with airborne magnetic images survey, and applying filters in the vertical derivative and analytical signal, lineaments in depth of this region discovered and investigated. 展开更多
关键词 GEOMAGNETIC GEOPHYSICAL Studies AIRBORNE Magnetic Images Haji abad
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Structural and Fault Analysis of Haji Abad with Interpretation of Landsat 8 Satellite Images
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作者 Shima Rahmati Kamel Mahmood Almasian +1 位作者 Mohsen Pourkermani Soraya Dana 《Open Journal of Geology》 2015年第6期470-488,共19页
Zagros orogenic belt has developed on northern-eastern edge of Arabian plate from Northern-Western-Southern-Eastern Turkey to Strait of Hormuz with a length of over 2000 km. Thick sedimentary series of the Zagros (6 -... Zagros orogenic belt has developed on northern-eastern edge of Arabian plate from Northern-Western-Southern-Eastern Turkey to Strait of Hormuz with a length of over 2000 km. Thick sedimentary series of the Zagros (6 - 12 km) has maintained complex tectonic history of the region, which represents all stages of development of a basin from a passive continental shelf to a rift. This finally represents various stages of deformation in relation to ophiolite obduction and continental collision. The study area is located in the south and southeastern part of Iran in the range of 28 and 29 to 55 and 57. The study area includes Hormozgan and Kerman Provinces in national classification. Geographic position of this region at the intersection of three sedimentary structural zones of Zagros, Makran and Central Iran has revealed that Hormozgan Province has specific geological and structural features. Nowadays, remote sensing techniques and particularly structural analysis with satellite images are supplement to the observation and field interpretation. Landsat satellites can be noted in this regard, which has helped the scientists to interprete natural science since a long time ago. Landsat 8 is equipped with panchromatic band and thus has a high spatial resolution. Therefore, the images obtained from this satellite are used. The images are raw and after application of various filters and image processing operations by ER mapper and Arc GIS the lineaments that have remained unidentified are observed. The discoveries are then introduced to the realm of construction geology in the form of a new map of regional faults using the remote sensing technologies. 展开更多
关键词 Haji abad LANDSAT 8 REMOTE Sensing STRUCTURAL ANALYSIS ER MAPPER 8
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An Improved Method for Seismic Site Characterization with Emphasis on Liquefaction Phenomena
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作者 Abbas Abbaszadeh Shahri Roshanak Rajablou Abdolvahed Ghaderi 《Open Journal of Earthquake Research》 2012年第2期13-21,共9页
Iran is an active seismic region and frequent earthquakes and because of the active faults, often leads to severe casual-ties caused by structural destruction. Earthquake damage is commonly controlled by three interac... Iran is an active seismic region and frequent earthquakes and because of the active faults, often leads to severe casual-ties caused by structural destruction. Earthquake damage is commonly controlled by three interacting factors, source and path characteristics, local geological and geotechnical conditions and type of the structures. Obviously, all of this would require analysis and presentation of a large amount of geological, seismological and geotechnical data. In this paper, nonlinear geotechnical seismic hazard analysis considering the local site effects was executed and the soil lique-faction potential analysis has been evaluated for the Nemat Abad earth dam in Hamedan province of Iran because of its important socioeconomic interest and its location. Liquefaction susceptibility mapping is carried out using a decisional flow chart for evaluation of earthquake-induced effects, based on available data such as geological, groundwater depth, seismotectonic, sedimentary features, in situ, field and laboratory geotechnical parameters. A series model tests were conducted and then on base of the achieved data the idealized soil profile constructed. A C# GUI computer code “NLGSS_Shahri” was Generate, developed and then employed to evaluate the variation of shear modulus and damping ratio with shear strain amplitude to assess their effects on site response. To verify and validate the methodology, the obtained results of the generated code were compared to several known applicable procedures. It showed that computed output of this code has good and suitable agreement with other known applicable procedures. 展开更多
关键词 Nemat abad Earth DAM “NLGSS_Shahri” GUI Computer Code Earthquake RECORD
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Monitoring Groundwater and Its Suitability for Drinking and Irrigation Purposes in the Sharif Abad Basin, Central Iran
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作者 Farzad Mohammadi Majid Ehteshami +1 位作者 Seyed Ahmad Mirbagheri Salman Tavassoli 《World Journal of Engineering and Technology》 2017年第3期487-506,共20页
The purpose of this study was to analyze the quality and quantity of groundwater in Sharif Abad Basin, located in Central Iran. The analysis of data collected from 24 observation wells indicated that the mean water ta... The purpose of this study was to analyze the quality and quantity of groundwater in Sharif Abad Basin, located in Central Iran. The analysis of data collected from 24 observation wells indicated that the mean water table has plummeted about 0.896 m/year during 19 years between 1993 and 2013 due to illegal groundwater pumping, which is the main cause of groundwater quality deterioration in this area. Seventeen samples were collected and analyzed to study physicochemical characteristics of groundwater such as pH, Hardness, Chloride (Cl), Electrical Conductivity (EC) and Total Dissolved Solids (TDS) values. The results were compared to drinking water quality standard published by World Health Organization (WHO) and it was concluded that none of the water samples?was?potable. The spatial distribution of anions and cations followed an increasing pattern eastwards towards the Salt Lake. The analysis of Hydrochemical facies of groundwater using Piper diagram indicated that the major type of groundwater in this basin is Na-Cl, which is mainly different from common type of fresh water in Iran (Ca-HCO3). Suitability of groundwater for irrigation purpose was assessed using Wilcox diagram and it was shown that all the water samples in 2013 belonged to C4S4 category, meaning they were not suitable for irrigation either. According to the ratio of chloride-bicarbonate, 100% of the samples were contaminated by saltwater intrusion. Also, the sodium-chloride ratio indicated that almost 60% of samples were contaminated by Halite solution. The presence of this solution proves the presence of salt domes in this area. 展开更多
关键词 GROUNDWATER Quality Wilcox DIAGRAM PIPER DIAGRAM Salinity Sharif abad BASIN
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Investigation of Glacial Effects and the Permanent Snow Lines on the Southern Slopes of the Hamadan Alvand Kuh
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作者 Abdullah Seif Ali Bazvand 《Journal of Geoscience and Environment Protection》 2018年第6期120-142,共23页
One of the issues that geomorphology researchers have focused on is to trace the geomorphic effects and the snow lines in the Quaternary glaciers in Iran. This research has been carried out in the valleys of the south... One of the issues that geomorphology researchers have focused on is to trace the geomorphic effects and the snow lines in the Quaternary glaciers in Iran. This research has been carried out in the valleys of the southern slopes of Alvand Kuh Mountains, namely the Serkan and Mobarakabad valleys. The equilibrium line altitude between current and ancient glaciers is one of the parameters that is used as a variation indicator of climate. There are several ways to rebuild this parameter (Ancient ELA). Equilibrium Line altitude requires the accurate knowledge of glacial geomorphic landforms and evidence. According to Kuhle, Hofer and Luis methods, providing and estimating the ancient and permanent snow lines were carried out in this research during field operations within several intervals and the glacial geomorphic evidences were identified such as circuses, sidebar moraines and terminal moraines. The six glacier subwatersheds were developed in a study area based on Kuhle, Hofer and Luis methods in which only two valleys of Serkan 3 and Mubarak Abad 3 had a high degree of accuracy in their ELA drop rate and old snow lines. It should be noted that the current snowlines of the current study area are 4400 m and 1400 m (based on the Schweitzer map). The results of the estimated snow lines for each of the six valleys are shown in Table 4. At the present study, the main of the Mobarak Abad valley area was divided to six glacial valleys which are called Serkan 1, 2, 3 and Mobarak Abad 1, 2, 3 and then several layers were provided, including cirque layers, moraine layers, primary and secondary crest layers, high crest layers, 10-meter DEM layer, highlight, different contour line layers and valleys profiles and finally, the ultimate map of glacial geomorphology of Serkan and Mobarak Abad valleys was prepared. 展开更多
关键词 Serkan and Mobarak abad VALLEYS of Alvand Kuh Kuhle Method GLACIAL Cirque QUATERNARY SNOW Line GLACIAL GEOMORPHOLOGY Map
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Studying Subsidence Coefficient of Mirages and Dynamic Storage Volume of Karstic Springs of Khorram Abad, West of Iran
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作者 Vidafar Milad Mohamad Reza Ahmadipour Reza Zarei Sahamiyeh 《Open Journal of Geology》 2016年第6期372-375,共4页
The studied subject is about subsidence coefficient of mirages and dynamic storage volume of Karstic springs in Khorram Abad in West of Iran. Subsidence coefficient indicates ability of groundwater discharge and hydro... The studied subject is about subsidence coefficient of mirages and dynamic storage volume of Karstic springs in Khorram Abad in West of Iran. Subsidence coefficient indicates ability of groundwater discharge and hydrologic properties of the environment;meaning effective porosity and transfer coefficient of springs. In general, in developed Karstic zones, each direct line of subsidence curve indicates a discharge regime. Obtained results from the study show that subsidence branch of Golestan, Motahari, Niloofar (changaei), Navekech, Dore Robat mirages have subsidence coefficient with mild slope and low value, which demonstrate passage of water through a seams system at the karst springs. Q and whirlpool stone mirages have two subsidence coefficients, which indicate passage of water through two seam systems in Karstic environment of springs. According to obtained results, process of changes in subsidence branch in these mirages has had at the first a mild slope and low discharge coefficient and in continue, its discharge would be declined with sharper slope and high discharge coefficient. In order to estimate dynamic storage volume of springs, MAILET general equation is applied, which is suitable for subsidence branch of hydrographs of centralized springs discharge. Following, dynamic storage volume of studied springs is analyzed and obtained results are presented in this study respectively. 展开更多
关键词 Subsidence Coefficient MIRAGE Dynamic Storage Karstic Springs Khorram abad
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Petrography and Mineralography of Emarat Lead and Zinc Deposit (South Arak)
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作者 Safoura Khani Ahamd Khakzad +1 位作者 Mehdi Safari Ali Solgi 《Open Journal of Geology》 2016年第6期387-398,共12页
Iran is host to more than 285 zinc and lead carbonated host deposits including world-class deposits such as Mehdi Abad and Irankouh. Emarat deposit is located in Sanandaj-Sirjan zone and Malayer-Isfahan metallogenic p... Iran is host to more than 285 zinc and lead carbonated host deposits including world-class deposits such as Mehdi Abad and Irankouh. Emarat deposit is located in Sanandaj-Sirjan zone and Malayer-Isfahan metallogenic province (northwestern part of Sanandaj-Sirjan tectonic zone). In the area, the mineralization has stratabound shape and restricted to Early Cretaceous limestones and dolomites. With investigation of the optical properties in microscopic survey and evaluation results of analysis, sphalerite, galena, pyrite and chalcopyrite were recognized as the main minerals, covellite, tennantite-tetrahydrite and serosities, quartz and barite as the secondary minerals and gyps, smithsonite were recognized as the minorminerals in the samples. Silicification alteration acted as major process in studied deposit and dolomitization, pyritization and hematitization also were observed in the deposit. Evidences show that mineralization has occurred in post diagenetic stage (epigenetic type) and after tectonic influence. 展开更多
关键词 Carbonated Host Deposits Mehdi abad Irankouh SILICIFICATION ALTERATION Diagenetic Stage
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Evaluation of Hydraulic Aperture of the Joints of Behesht Abad Dam Foundation, Iran
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作者 Ameneh F. Dardashti Rasoul Ajalloeian 《Open Journal of Geology》 2015年第6期375-382,共8页
The fluid flow in rock fractures during shear processes has been an important issue in rock mechanics. During shearing, because of the stiffer rock matrix, most deformation occurs in the joints, in the form of normal ... The fluid flow in rock fractures during shear processes has been an important issue in rock mechanics. During shearing, because of the stiffer rock matrix, most deformation occurs in the joints, in the form of normal and shear displacement. Since the joints are rough, deformations will also change the joint aperture and fluid flow. In this article, the hydraulic apertures of the joints of dolomitic limestone foundation of Behesht Abad dam are investigated. Firstly, geological data during a site investigation phase in dam site is gathered, and then related calculations are done and the hydraulic conductivity of the joints is evaluated. The results indicate that the hydraulic conductivity of the joints is reasonable. 展开更多
关键词 Behesht abad DAM HYDRAULIC APERTURE HYDRO-MECHANICAL BEHAVIOR SEEPAGE
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Aβ结合乙醇脱氢酶与阿尔茨海默病 被引量:1
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作者 王明宇 杨宇 吴江 《中风与神经疾病杂志》 CAS CSCD 北大核心 2011年第7期663-665,共3页
近年来,线粒体功能障碍(Mitochondrial dysfunction)对包括阿尔茨海默病(Alzheimer disease,AD)在内的中枢神经系统退行性疾病的影响日益引起科研人员的关注。Aβ结合乙醇脱氢酶(Aβ binding alcohol dehydrogenase,ABAD)是一种... 近年来,线粒体功能障碍(Mitochondrial dysfunction)对包括阿尔茨海默病(Alzheimer disease,AD)在内的中枢神经系统退行性疾病的影响日益引起科研人员的关注。Aβ结合乙醇脱氢酶(Aβ binding alcohol dehydrogenase,ABAD)是一种能与β-淀粉样肽(β-amyloid peptide,Aβ)特异性结合的线粒体酶,诸多的研究结果显示ABAD与AD的发生、发展有着密切关系,本文就其研究进展综述如下。 展开更多
关键词 阿尔茨海默病 乙醇脱氢酶 中枢神经系统退行性疾病 线粒体功能障碍 peptide Β-淀粉样肽 abad
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不同龄小鼠内耳中ABAD的表达及临床意义
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作者 汪晓锋 林昶 程金妹 《山东大学耳鼻喉眼学报》 CAS 2008年第3期209-211,共3页
目的探讨ABAD(AmyloidβBinding Alcohol Dehydrogenase)在不同龄小鼠内耳中表达量上的差异。方法将昆明小鼠分为2周龄、5个月龄、1年3个不同的年龄组,采用逆转录聚合酶链反应(RT-PCR)技术检测ABAD在不同龄小鼠内耳中表达的动态变化。结... 目的探讨ABAD(AmyloidβBinding Alcohol Dehydrogenase)在不同龄小鼠内耳中表达量上的差异。方法将昆明小鼠分为2周龄、5个月龄、1年3个不同的年龄组,采用逆转录聚合酶链反应(RT-PCR)技术检测ABAD在不同龄小鼠内耳中表达的动态变化。结果ABAD在不同年龄组小鼠内耳中均有表达,每组之间差异有统计学意义(P<0.05),且随着年龄的增大ABAD在内耳的表达逐渐增多。结论ABAD可能参与内耳细胞的老化机制;与老年性耳聋的发生有关。 展开更多
关键词 abad 小鼠 内耳
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针灸对AD模型大鼠海马神经元线粒体能量代谢相关蛋白的影响 被引量:20
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作者 罗磊 孙国杰 杜艳军 《中国针灸》 CAS CSCD 北大核心 2013年第10期913-918,共6页
目的:探讨针灸治疗阿尔茨海默病(AD)的作用机制,为临床针灸防治AD提供实验依据。方法:采用Wistar大鼠,SPF级、雄性80只,体质量(200±20)g,随机分为正常组、假手术组、模型组和针灸治疗组,每组20只。运用大鼠海马注射Aβ1-42的模型... 目的:探讨针灸治疗阿尔茨海默病(AD)的作用机制,为临床针灸防治AD提供实验依据。方法:采用Wistar大鼠,SPF级、雄性80只,体质量(200±20)g,随机分为正常组、假手术组、模型组和针灸治疗组,每组20只。运用大鼠海马注射Aβ1-42的模型复制方法复制模型。除针灸治疗组外其余各组相应处理后均常规饲养,针灸治疗组针灸"百会"和"肾俞"穴,每日1次,7天为一疗程,治疗2个疗程,疗程间休息1日。运用免疫组化和定量RT-PCR技术分别检测AD模型大鼠海马神经元线粒体中Aβ-结合性乙醇脱氢酶蛋白(ABAD)和细胞色素氧化酶Ⅳ(COXⅣ)的水平。结果:针灸能有效下调大鼠海马神经元线粒体中ABAD的水平,提高COXⅣ的活性,与模型组比较差异具有统计学意义(P<0.01),与正常组和假手术组比较差异无统计学意义(P>0.05)。提示针灸能有效抑制ABAD的过度表达、提高COXⅣ的活性,减少活性氧自由基(ROS)的漏出,改善线粒体能量代谢障碍,从而达到防治AD的目的。结论:针灸防治AD可能与其抑制海马神经元线粒体中ABAD的过度表达、提高COXⅣ的活性从而改善线粒体能量代谢有关。 展开更多
关键词 阿尔茨海默病 针灸 Aβ-结合性乙醇脱氢酶蛋白 细胞色素氧化酶Ⅳ
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ABAD、雌激素受体、bcl-2、Bax在小鼠内耳中的表达差异 被引量:3
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作者 汪晓锋 林昶 程金妹 《中华耳科学杂志》 CSCD 2008年第4期454-458,共5页
目的探讨β淀粉样蛋白醇脱氢酶(amyloidβbinding alcohol dehydrogenase,ABAD)、雌激素受体(estrogen receptors,ERs)、bcl-2、Bax(Bcl-associated X protein,Bcl相关X蛋白)在不同龄小鼠内耳中表达量上是否存在线性变化趋势。方法将昆... 目的探讨β淀粉样蛋白醇脱氢酶(amyloidβbinding alcohol dehydrogenase,ABAD)、雌激素受体(estrogen receptors,ERs)、bcl-2、Bax(Bcl-associated X protein,Bcl相关X蛋白)在不同龄小鼠内耳中表达量上是否存在线性变化趋势。方法将昆明小鼠分为2周龄、5月龄、1年三组,每组10只,采用免疫组织化学方法检测ABAD、ERs、bcl-2、Bax在不同年龄组小鼠内耳中的表达情况。结果ABAD、ERs、bcl-2、Bax在不同龄小鼠内耳螺旋神经节细胞中均有表达,且随着年龄增大,ABAD、Bax表达逐渐增多,ERs、bcl-2表达逐渐减少,均具有统计学意义(P<0.05)。结论ABAD随年龄增大表达有增多的趋势,可能参与了内耳细胞的老化机制,可能与老年性耳聋的发生有关。 展开更多
关键词 β淀粉样蛋白醇脱氢酶(abad) 小鼠 内耳
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论固定语中ABAD结构 被引量:1
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作者 钟武媚 《玉林师范学院学报》 2004年第2期95-97,共3页
固定语中ABAD结构在现代汉语词汇中占有一定比重,其结构特点、语法功能和语义都有独特一面,值得研究。本文试图在这几个方面作一些粗略探讨。
关键词 固定语 abad结构 现代汉语 语法功能 语义 成语 惯用语 词汇
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Investigation on the Geochemical Distribution of REE and Heavy Metals in Western Part of Jalal-Abad Iron Ore Deposit, Zarand, SE of Iran
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作者 Abdollah Yazdi Sajad Ziaaldini Rahim Dabiri 《Open Journal of Ecology》 2015年第9期460-476,共17页
The Jalal-Abad iron ore deposit, with a reserve of more than 200 Mt ore, is located in NW of Zarand region, southeastern Iran. The ore deposit occurs in the form of an elongated lens-shaped body incorporated in a fold... The Jalal-Abad iron ore deposit, with a reserve of more than 200 Mt ore, is located in NW of Zarand region, southeastern Iran. The ore deposit occurs in the form of an elongated lens-shaped body incorporated in a folded structure of Rizu volcano-sedimentary unit. Mineralization occurred mainly in siltstones, acidic volcanic rocks and dolomitized limestones. The ore minerals include magnetite, hematite, pyrite, chalcopyrite, goethite, malachite and azurite. Chloritization and silicification are the two most widespread alteration types in the Jalal-Abad area. Cu and Ti are among the associated elements with iron in the ore samples. In comparison, the concentrations of Cu, Ti and REE are relatively low in the samples analyzed. The combined concentrations of Ce, La and Y show that geochemical background values for most areas have been measured. The Pearson correlation coefficient values and the results of cluster and principal component analyses indicate a strong correlation between REE, La, Ce, and Y with Sr in the same geochemical group suggesting a common source for these elements. A close association between Cu and Cl with metasomatic host rock and among Pb, Zn and Ba with carbonate host rocks is observed. 展开更多
关键词 REE HEAVY Metals Iron ORE DEPOSIT Jalal-abad Zarand
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Economic Geology Study with Focus on Cu Mineralization (Fath Abad Area, Khorasan Razavi)
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作者 Reza Ehsani Naghmeh Mohamadi Mohamd Hossain Ehsani 《Journal of Geoscience and Environment Protection》 2016年第10期1-27,共27页
The study area is located in south of Khorasan-Razavi province. This region is part of central Iran, which is of high significance due to existence of metal and non-metal deposits. Considering the location of the stud... The study area is located in south of Khorasan-Razavi province. This region is part of central Iran, which is of high significance due to existence of metal and non-metal deposits. Considering the location of the study area, the lithology and metal-mineral indices of Dolat-Abad sheet, it can be said that their metallic deposits are economically feasible. Hence, this region is considered promising for exploration of metallic deposits. In order to achieve this goal, at first, the region’s alterations were detected by using OLI-ASTER images in ENVI software. After processing the lithogeochemical data in SPSS and GIS software, anomaly map of studied elements was obtained. Using geological map, different layers such as lithology, faults and indices were obtained. Eventually, by preparing the layers and incorporating those using the index overlay GIS modeling, promising mineralization areas in the region were identified. Results show that a significant Cu-Au mineralization has occurred in the south western region of the study area. 展开更多
关键词 Fath-abad Khorasan-Razavi Copper GEOCHEMICAL Remote Sensing
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可分泌表达Aβ阻断肽融合基因原核表达载体的构建
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作者 杨宇 杨欣 +2 位作者 孙欣 吴昊 吴江 《中国老年学杂志》 CAS CSCD 北大核心 2010年第18期2609-2612,共4页
目的构建神经营养素3(NT3)信号肽、Aβ1-42阻断肽ABAD-DP、溶源肽HA2及穿膜肽TAT融合基因的原核表达载体pBV220/NAHT,为将Aβ1-42阻断肽ABAD-DP用于AD基因治疗提供实验基础。方法采用非对称互补引物/模板法,分别制备两端含有酶切位点的A... 目的构建神经营养素3(NT3)信号肽、Aβ1-42阻断肽ABAD-DP、溶源肽HA2及穿膜肽TAT融合基因的原核表达载体pBV220/NAHT,为将Aβ1-42阻断肽ABAD-DP用于AD基因治疗提供实验基础。方法采用非对称互补引物/模板法,分别制备两端含有酶切位点的ABAD-DP和HA2-TAT的cDNA,将其连接到NT3的信号肽3′端,组成融合基因NT3-ABAD-DP-HA2-TAT(NAHT),再将该融合基因亚克隆于原核表达载体pBV220,构建原核表达载体pBV220/NAHT。结果经DNA测序证实成功克隆ABAD-DP和HA2-TATcDNA;经限制性内切酶酶切证实成功将ABAD-DP和HA2-TAT基因重组到NT3信号肽的3′端,并将融合基因亚克隆于pBV220载体内。结论成功构建了表达NT3信号肽、阻断肽ABAD-DP、融源肽HA2及穿膜肽TAT融合基因的原核表达载体pBV220/NAHT。 展开更多
关键词 神经营养素3 abad-DP 基因治疗 原核表达载体
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分泌表达Aβ阻断肽的重组腺相关病毒的构建
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作者 王明宇 杨宇 +4 位作者 吴江 王旭 车丽赫 孙欣 杨弋 《中风与神经疾病杂志》 CAS CSCD 北大核心 2012年第4期292-296,共5页
目的构建可稳定分泌表达ABAD-DP的重组腺相关病毒系统,并在细胞水平研究它与Aβ42的结合能力。方法应用分子克隆技术构建含有融合基因NT4-TAT-6His-ABAD-DP(NTA)的腺相关病毒载体。应用磷酸钙共沉淀法进行病毒包装。应用免疫细胞化学方... 目的构建可稳定分泌表达ABAD-DP的重组腺相关病毒系统,并在细胞水平研究它与Aβ42的结合能力。方法应用分子克隆技术构建含有融合基因NT4-TAT-6His-ABAD-DP(NTA)的腺相关病毒载体。应用磷酸钙共沉淀法进行病毒包装。应用免疫细胞化学方法检测重组病毒在Hela细胞中的表达。应用免疫荧光技术检测重组病毒与Aβ42在Hela细胞中的结合作用。结果 pGEM-T Easy/ABAD-DP经EcoR I酶切后,可以得到96bp的目的片段,ABAD-DP基因经DNA测序证实与GenBank序列一致;重组质粒pSSGH/NTA经EcoRⅠ与BamHⅠ联合酶切,可以得到384bp的目的片段;成功获得滴度为3.01×109PFU/ml的重组病毒;含有6×His标签的重组病毒在Hela细胞中实现表达;通过免疫荧光技术,可以分别观察到重组病毒组、Aβ42组和重组病毒+Aβ42组的绿色、红色和黄色荧光。结论成功获得能够稳定表达ABAD-DP并能够在细胞内与Aβ42结合的重组腺相关病毒,为阿尔茨海默病的治疗提供新的思路。 展开更多
关键词 abad阻断肽 基因克隆 病毒包装 表达研究
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过表达ABAD对Aβ_(1-42)刺激的293T细胞线粒体功能和自噬水平的影响 被引量:1
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作者 洪婷婷 张宇 崔理立 《海南医学》 CAS 2021年第11期1365-1369,共5页
目的探讨β淀粉样蛋白1-42(Aβ_(1-42))刺激下,在293T细胞中过表达淀粉样蛋白结合醇脱氢酶(ABAD)对线粒体功能及自噬相关标记蛋白水平的影响。方法将正常培养的293T细胞随机分为:(1)Control组、过表达ABAD组、Control给药组(Aβ_(1-42)5... 目的探讨β淀粉样蛋白1-42(Aβ_(1-42))刺激下,在293T细胞中过表达淀粉样蛋白结合醇脱氢酶(ABAD)对线粒体功能及自噬相关标记蛋白水平的影响。方法将正常培养的293T细胞随机分为:(1)Control组、过表达ABAD组、Control给药组(Aβ_(1-42)5μmol/L、10μmol/L、20μmol/L)、过表达ABAD给药组(Aβ_(1-42)5μmol/L、10μmol/L、20μmol/L);(2)Control组、sh-ABAD组;(3)Control+DMSO组、Control+CCCP组、过表达ABAD+DMSO组、过表达ABAD+CCCP组(WT/Aβ10μmol/L)。三磷酸腺苷(ATP)试剂盒测定Control组、过表达ABAD组、Control给药组、过表达ABAD给药组细胞的ATP含量;活性氧(ROS)试剂盒测定活性氧生成量。Westernblot法检测Control+DMSO组、Control+CCCP组、过表达ABAD+DMSO组、过表达ABAD+CCCP组线粒体自噬相关蛋白表达水平。通过标准曲线换算ATP细胞含量和活性氧生成量,采用ImageJ对Westernblot条带进行量化。结果Control组与过表达ABAD组ATP含量和活性氧生成量变化不明显,差异无统计学意义(P>0.05);与Control给药组相比,在Aβ_(1-42)(10μmol/L)刺激后,过表达ABAD给药组的ATP含量减少,差异具有统计学意义(P<0.05),但两组的活性氧生成量变化不明显,差异无统计学意义(P>0.05);与Control+CCCP组相比,过表达ABAD+CCCP组在Aβ_(1-42)10μmol/L诱导后线粒体自噬相关蛋白P62降低、LC3B升高,差异均有统计学意义(P<0.05)。结论过表达ABAD不影响293T细胞的线粒体功能,但在特定浓度Aβ_(1-42)刺激下可影响线粒体ATP产生及促进线粒体自噬发生。 展开更多
关键词 淀粉样蛋白结合醇脱氢酶 Β-淀粉样蛋白 三磷酸腺苷 活性氧 线粒体自噬
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ABAD/HADH Ⅱ在喉鳞状细胞癌与声带息肉组织中的表达
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作者 张晖萍 张鹏飞 +1 位作者 上官翰京 兰书湛 《山东大学耳鼻喉眼学报》 CAS 2006年第5期444-446,共3页
目的:检测喉癌组织中ABAD/HADHⅡ的表达,探讨ABAD/HADHⅡ在喉鳞状细胞癌增殖中的作用。方法:选用福建医科大学附属第一医院2002—2005年的27例喉癌手术标本及12例声带息肉手术标本,采用免疫组化检测ABAD/HADHⅡ的表达及分布情况... 目的:检测喉癌组织中ABAD/HADHⅡ的表达,探讨ABAD/HADHⅡ在喉鳞状细胞癌增殖中的作用。方法:选用福建医科大学附属第一医院2002—2005年的27例喉癌手术标本及12例声带息肉手术标本,采用免疫组化检测ABAD/HADHⅡ的表达及分布情况。结果:ABAD/HADHⅡ主要表达在鳞状细胞癌的细胞浆,在生长活跃的细胞表达较高;ABAD/HADHⅡ在喉鳞状细胞癌组织中的表达比在息肉组织中的表达高(P〈0.05)。结论:ABAD/HADHⅡ在喉癌组织中表达较正常组织高,与喉鳞状细胞癌的增殖有一定的关系。 展开更多
关键词 喉肿瘤 增殖 β淀粉样蛋白乙醇脱氢酶/L-3-羟酰基辅酶A脱氢酶
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